FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219384
Sequences flagged as poor quality0
Sequence length150
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGACGCGGTATTGTCAGGAGCTAACTGGGATACTGCTCTATGAGACC129045.881923932465448No Hit
CATAGACCTGGTACTTCTCTTCTGGGTCCATTTTTGGTTCTCTTCTATGA101494.626134996171097No Hit
CATAGAACTGAGGCTGGTAGAGGGAGGCGTGGTATGAGGGGAATCTATGA96964.41964774094738No Hit
CATAGATTACTGGGAGACTGGCGCTCACCTCTATGAGACCAAGTCTCTGC91034.149345439959158No Hit
CATAGAACTTGTGCTTTTGGTGTCACCCGTAATCCCTCCCTTTTCTATGA79763.6356343215548996No Hit
CATAGAGGGAAGAGAAGAGGAGCTGACATCCCAACCACACGTCTATGAGA69733.1784451008277723No Hit
CATAGAAGCTGTCGTTTGTCAACTCCTGGGCCCATAATACACTTTTCTAT63182.879881851000985No Hit
CATAGACAGTCCTGCCGTGTACATGTACAGCCTACATCCCTCATCTATGA56862.591802501549794No Hit
CATAGATGTCCCCATTCATCCTGCAAACAGAATGGGACCCATCTATGAGA54372.4783028844400685No Hit
CATAGAGGATCTCAGGAGTTTAGGTGGGAGGAACAGGGAGATGTTCTATG42051.9167304817124313No Hit
CATAGACAGTATGGGGGAAACGGCTCTCTTCCGCCTTTATCCTTCTATGA37701.718448018087007No Hit
CATAGATTACTGGAGACAGCCGCTCACCTCTATGAGACCAAGTCTCTGCT36091.6450607154578272No Hit
CATAGAAAAGTCACCATGCAGCCCTGGCAAAAGGGTAAGAGTTCTATGAG32061.4613645480071473No Hit
CATAGACGCGGTATTGTCAGGAGCTGACTGGGATACTGCTCTATGAGACC25811.176475950844182No Hit
CATAGAATTGCCTACTTTGGGACCCATCTATGAGACCAAGTCTCTGCTAC24351.109925974546913No Hit
CATAGAGCACCCAACTATCCTTCAGCATGAGAAGAATGGCCTCTATGAGA21550.9822958830179047No Hit
CATAGATCCAGGGAGCTTTCAAGTGTCGAACAAGGGCATGGTCAAGTGTC20930.9540349341793385No Hit
CATAGAAGCTGTCGTTTGTCAACTCCTATAGATTATGCTCTCTTCCGCCT19340.8815592750610801No Hit
CATAGAAAAGTCACCATGCAGCCCTGGGCCCATAATACACTTTTCTATGA18700.8523866827115925No Hit
CATAGAAAAGTCACCATGCAGCCCTGTGATTGTCCTTGCACACCATGGAT18570.8464609998906027No Hit
CATAGAGGCAAGCTAACTCTGAGATCCATCAGAGCATCACCCCTTCTATG17720.8077161506764395No Hit
CATAGAAGCTGTCGTTTGTCAACTCCTCCTGGGCCCATAATACACTTTTC17560.8004230025890676No Hit
CATAGAGGTTCTTTGTAGACTTACTTGGCATGAGGTTCTTCAACACAGCT16290.742533639645553No Hit
CATAGAAGGGCAATTATCTCATCCTAGACAGAAGGGCAAGTGTATGGTCA16110.7343288480472596No Hit
CATAGAAAAGTCACCATGCAGCCCTGCCGTGTACAGCCTACATCCCTCAT16050.7315939175144951No Hit
CATAGAAAAGTCACCATGCAGCCCTGCCCTTGTCCTTGCACACCATGGAT15170.6914816030339497No Hit
CATAGAGCAGTGCAGCAGAAGATAGCCAGTGTACAAACCAAACAATAGCC13970.6367829923786602No Hit
CATAGATTACTGGGAGACAGCCGCTCACCTCTATGAGACCAAGTCTCTGC13930.6349597053568172No Hit
CATAGACGTCTTTTGTGGGTTTTCTTTCTTGAGGTTCTTCAACACAGCTA13730.6258432702476024No Hit
CATAGAATTCCCCAATGTCCTGTGCAAACAGAATGGGACCCATCTATGAG12890.5875542427888999No Hit
CATAGACTCCTGAGTTCAAGATTCTACCCACAGCCCAGTTTTCCTTCTCT11250.5127994748933378No Hit
CATAGATCATGGGACTTCAGCATGGCTCTCTTCCGCCTTTATCCTTCTAT11210.5109761878714947No Hit
CATAGAATCCCTGCCCTTGTCCTTGCACACCATGGATCTATGAGACCAAG11030.5027713962732013No Hit
CATAGATCATGGGACTTCAGCATGGCAAAAGGGCAAGTGTATGGTCAGTT11010.5018597527622798No Hit
CATAGATCCAGGGAGCTTTCAAGTGTCTAGAACAAGGGCATGGTCAAGTG10890.4963898916967509No Hit
CATAGAGGTTCTTTGTAGACTTACTTGGCAAAAGGGTAAGAGTTCTATGA10580.48225941727746785No Hit
CATAGATCATGGGACTTCAGCATGGCGTCAGTGTCCCCTTCATTTTGCTG10480.4777011997228604No Hit
CATAGACAGAACACCACACCCCTGTCCTTGTCCTTGCACACCATGGATCT10130.46174743828173426No Hit
CATAGAAAAGTCACCATGCAGCCCTGGTGGTGCTGGAACTAGTTCTATGA10120.4612916165262736No Hit
CATAGAAGGGCAATTATCTCATCCTAGACGAAGGGCAAGTGTATGGTCAG10070.45901250774896984No Hit
CATAGAGACCCCCAAGTAGCCTAACTGGGATTTGTCCTTGCACACCATGG9980.45491011194982317No Hit
CATAGATGTGTGAGAGAGGGAGAGACAGCCTTCTCTGATACCATCCCTCT9950.4535426466834409No Hit
CATAGATGCACCAGAAGCCACCTACCAAGTATATGACACATTACACCAGT9850.44898442912883346No Hit
CATAGAGGTTCTTTGTAGACTTACTTGGCAACACTGACGACAAGGTTTTC9420.42938409364402147No Hit
CATAGAATTGCCTACTTTGGGACCCATCTATGCAAACAGAATGGGACCCA9390.4280166283776392No Hit
CATAGAACTTGTGCTTTTGGTGTCACTTGATCATCCATTGTCTGCTCTAT9370.4271049848667177No Hit
CATAGAATCCCTGCCCTTGACCTTGGACACCCGTAATCCCTCCCTTTTCT9280.42300258906757104No Hit
CATAGATCCAGGGAGCTTTCAAGTGTCTATGAGAACAAGGGCATGGTCAA8960.40841629289282716No Hit
CATAGATCATGAATGACTCCCACTCTGTAGATTATGCTCTCTTCCGCCTT8840.4029464318272983No Hit
CATAGAAGCTGTCGTTTGTCAACTCCTGGCAAAAGGGTAAGAGTTCTATG8790.40066732304999453No Hit
CATAGAATTGCCTACTTTGGGACCCATCTATGAGACCAAGTCTCTGCAAA8580.3910950661853189No Hit
CATAGATGCACCAGAAGCCACCTACCGTATGATCTAGCTTCGGAATGTTT8260.37650877001057503No Hit
CATAGACGCGGTATTGTCAGGAGCTGACATCCCAACCACACGTCTATGAG8220.37468548298873205No Hit
CATAGAACTGAGGCTGGTAGAGGGAGCCTTGGAGGCCTAGCCTGGCAGCT7900.3600991868139882No Hit
CATAGACCCACTTACACACACAGGTTTGGGGGAAGAGAAACAGAATCTAT7880.3591875433030668No Hit
CATAGAATCCCTGCCCTTGACCTTGGTGGTTCCAACTCTATAGATTATGC7860.35827589979214525No Hit
CATAGAATTGCCTACTTTGGGACCCATCTATGAGACCAAGTCTGCAAACA7680.35007110819385184No Hit
CATAGAAAAGTCACCATGCAGCCCTGTGAGGTTCTTCAACACAGCTATTC7670.3496152864383911No Hit
CATAGAGGTTCTTTGTAGACTTACTTGGCAAAAGGGCAAGTGTATGGTCA7630.34779199941654815No Hit
CATAGATGGCATGTCACTTCCTCAGAGCATCACCCCTTCATTTTGCTGGA7570.3450570688837837No Hit
CATAGAAAAGGGCGGTGGGAAAGTTCTATGAGATGGTGAAAAGTAGGGGA7430.33867556430733325No Hit
CATAGATCTGGGAGGTGAAGAGGACATCTGGGAGGAACAGGGAGATGTTC7400.337308099040951No Hit
CATAGAATTGCCTACTTTGGGACCCATCTATGAGACCAAGTGCAAACAGA7310.3332057032418043No Hit
CATAGATCCAGGGAGCTTTCAAGTGTCTATACTCCAGTCTCGGCAACAAG7290.33229405973088283No Hit
CATAGAGGCAAGCTAACTCTGAGATCCACAGCCCAGTTTTCCTTCTCTAT6820.31087043722422786No Hit
CATAGAATTCCCCAATGTCCTGTGCACACCATGGATCTATGAGACCAAGT6740.3072238631805419No Hit
CATAGAACTTTCAGTAATAGGGGACAAGAACAAGGGCATGGTCAAGTGTC6680.30448893264777743No Hit
CATAGACAGAACACCACACCCCTGTCCATAGAAGGGCAAGTGTATGGTCA6610.3012981803595522No Hit
CATAGACTTTCTGAAATTGTTTGCTGCTTTGGGGGAAGAGAAACAGAATC6590.3003865368486307No Hit
CATAGAATTGCCTACTTTGGGACCCAGCATGAGAAGAATGGCCTCTATGA6510.29673996280494475No Hit
CATAGAGACCCCCAAGTAGCCTAACTGGGATACGCTCTCTTCCGCCTTTA6350.28944681471757283No Hit
CATAGATTACTGGGAGACTGGCGCTCTCTTCCGCCTTTATCCTTCTATGA6340.2889909929621121No Hit
CATAGATGTGTGAGAGAGGGAGAGACAGGAATTAGAGCCAGTTCTGAGTC6310.28762352769572985No Hit
CATAGAGGGAAGAGAAGAGGAGCTGCCTGGGCCCATAATACACTTTTCTA6130.27941873609743645No Hit
CATAGATGTCCCCATTCATCCTGCAACCGTAAGCCTTCTCTGATACCATC6070.276683805564672No Hit
CATAGAAGGGCAATTATCTCATCCTAGACCATAGAAGGGCAAGTGTATGG5860.26711154869999637No Hit
CATAGAATTGCCTACTTTGGGACCCATGCAAACAGAATGGGACCCATCTA5820.2652882616781534No Hit
CATAGACCCCCATGCAACCACATCTAGGGCAAGTGTATGGTCAGTTCTAT5780.2634649746563104No Hit
CATAGAATTGCCTACTTTGGGACCCATCTGCAAACAGAATGGGACCCATC5570.25389271779163475No Hit
CATAGAATCCCTGCCCTTGACCTTGGGGGAAGAGAAACAGAATCTATGAG5510.2511577872588703No Hit
CATAGATGCACCAGAAGCCACCTACCGTACCAAGTATATGACACATTACA5410.24659956970426283No Hit
CATAGACAGAACACCACACCCCTGTCCTTGCACACCATGGATCTATGAGA5370.24477628268241985No Hit
CATAGACCATGTAATACCATCGTGAGCAGGGGCAATTATCTCAACTCCCA5260.23976224337235166No Hit
CATAGAATCCCTGCCCTTGACCTTGCACACCATGGATCTATGAGACCAAG5200.2370273128395872No Hit
CATAGAATTCCCCAATGTCCTTGCACACCATGGATCTATGAGACCAAGTC5150.23474820406228347No Hit
CATAGAACTTGTGCTTTTGGTGTCACAAGCACATTATTATTGGTTGGTTC5130.23383656055136198No Hit
CATAGAATTGCCTACTTTGGGACCCATCTATGAGCCCCTTTACCTACCTT4930.22472012544214712No Hit
CATAGAGACCCCCAAGTAGCCTAACTGTGCCCTGTGATTGTCCTGGTCTA4540.20694307697917808No Hit
CATAGACCCCCATGCAACCACATCTACACTGACGACATGGTTCTACACAT4530.20648725522371733No Hit
CATAGAGTCATTCCAAACTGTGCACTTGTCCTTGCACACCATGGATCTAT4390.2001057506472669No Hit
CATAGAGGTTCTTTGTAGACTTACCTGGCAAAAGGGTAAGAGTTCTATGA4180.19053349378259124No Hit
CATAGAGAAAGGCAGGCGAAGTTAGCCCCTTTTGCCAAGATTGTAGGAGT4140.1887102067607483No Hit
CATAGATGCACCAGAAGCCACCTACCTACACATAGAATCACTTGATCATC4000.18232870218429784No Hit
CATAGAAGCTGTCGTTTGTCAACTCCTGACAGTTTGGGGGAAGAGAAACA3980.18141705867337637No Hit
CATAGAAGCTGTCGTTTGTCAACTCCTGATCTAGCTTCGGAATGTTTTCA3910.17822630638515116No Hit
CATAGACCATGTAATACCATCGTGAGCAGTGTGCAAACAGAATGGGACCC3900.17777048462969042No Hit
CATAGAATTGCCTACTTTGGGACCCATCTATGAGACTGCAAACAGAATGG3740.1704773365423185No Hit
CATAGAAGGGCAATTATCTCATCCTAGACCAAGTATATGACACATTACAC3730.17002151478685776No Hit
CATAGACCTGGTACTTCTCTTCTGGGTACACTGATCTAGCTTCGGAATGT3550.16181672318856435No Hit
CATAGAAGCTGTCGTTTGTCAACTCCTACACTGGTGGTGCTGGAACTAGT3520.1604492579221821No Hit
CATAGACAGTCCTGCCGTGTACATGTGTACAGCCTACATCCCTCATCTAT3520.1604492579221821No Hit
CATAGAATTGCCTACTTTGGGACCCATCTATGAGACCAAGTCTCTGCTGC3490.1590817926557999No Hit
CATAGAAAAGGGCGGTGGGAAAGTTCTATGGTGAAAAGTAGGGGACAGTT3490.1590817926557999No Hit
CATAGAAAAGTCACCATGCAGCCCTGGTTCTACACATAGAACAAGGGCAT3440.15680268387849616No Hit
CATAGACAGAACACCACACCCCTGTCTATGAGACCTTAGGAGTTCTGGCC3390.15452357510119244No Hit
CATAGACCATGTAATACCATCGTGAGCAGCACCCCTTCATTTTGCTGGAA3320.15133282281296723No Hit
CATAGACCCCCATGCAACCACATCTAGACATGCATCGGCCGCACTGAAAT3310.15087700105750645No Hit
CATAGAGGATCTCAGGAGTTTAGGTGGGTCCATTTTTGGTTCTCTTCTAT3280.14950953579112425No Hit
CATAGAGACCCCCAAGTAGCCTAACTGGGAGGAACAGGGAGATGTTCTAT3110.14176056594829156No Hit
CATAGAATCCCTGCCCTTGACCTTGGCATAGATCTGGTGGTGCTGGAACT3060.13948145717098787No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG3020.13765817014914486No Hit
CATAGACTTTCTGAAATTGTTTGCTGCTTTGTCCTTGCACACCATGGATC3020.13765817014914486No Hit
CATAGACCCACTTACACACACAGGTTTTCCGGCTTGAGGTTCTTCAACAC3000.1367465266382234No Hit
CATAGAAAAGGGCGGTGGGAAAGTTCTATGAGACCAAGTCTCTGGTGAAA2950.13446741786091965No Hit
CATAGAAGCTGTCGTTTGTCAACTCCTATCTCAACTCCCAGCTCCTTCTA2920.13309995259453744No Hit
CATAGAGGCAAGCTAACTCTGAGATCCACTTTGTTCCCTGGCTTCTCTAT2910.1326441308390767No Hit
CATAGACAGAACACCACACCCCTGTCTATGAGACCAAGTCTCTGCTACCG2790.12717426977354773No Hit
CATAGATCATGAATGACTCCCACTCTGTTCCCTGGCAAAAGGGTAAGAGT2720.12398351748532253No Hit
CATAGATCTGGGAGGTGAAGAGGACACGTCTGAACTCCAGTCAGGATGAC2460.11213215184334319Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CATAGAGACCCCCAAGTAGCCTAACTGGGAGGCACCCCCCAGTAGGGGCA2450.11167633008788243No Hit
CATAGAACTTGTGCTTTTGGTGTCACAGCCCAGTTTTCCTTCTCTATGAG2350.10711811253327498No Hit
CATAGATCCAGGGAGCTTTCAAGTGTCTATGAGACCAAGTCTCTGCTACC2350.10711811253327498No Hit
CATAGAGGGAAGAGAAGAGGAGCTGCTACCGTACCCCTTCATTTTGCTGG2330.10620646902235349No Hit
CATAGAGAAAGGCAGGCGAAGTTAGCCTTCTCTGATACCATCCCTCTATG2280.10392736024504978No Hit
CATAGAAGCTGTCGTTTGTCAACTCCTATCACTTGATCATCCATTGTCTG2270.10347153848958904No Hit
CATAGAATTCCCCAATGTCCTGTGCACCCCGGGCCAAGCCTCTCCTCCCC2230.10164825146774606No Hit
CATAGAAAAGTCACCATGCAGCCCTGCCCTGTGATTGTCCTGGTCTATGA2220.1011924297122853No Hit
CATAGAAAAGGGCGGTGGGAAAGTTCTATGAGACCAAGTCTCTGCTAAGG2210.10073660795682457No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)