FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219384
Sequences flagged as poor quality0
Sequence length150
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGCAGTATCCCAGTTAGCTCCTGACAATACCGCGTCTATGTGTAG131445.991321153776028No Hit
CATAGAAGAGAACCAAAAATGGACCCAGAAGAGAAGTACCAGGTCTATGT102084.6530284797432815No Hit
CATAGATTCCCCTCATACCACGCCTCCCTCTACCAGCCTCAGTTCTATGT97154.428308354301134No Hit
CATAGAGGTGAGCGCCAGTCTCCCAGTAATCTATGTGTAGAACCATGTCG89064.059548554133392No Hit
CATAGAAAAGGGAGGGATTACGGGTGACACCAAAAGCACAAGTTCTATGT80823.683951427633738No Hit
CATAGACGTGTGGTTGGGATGTCAGCTCCTCTTCTCTTCCCTCTATGTGT68153.1064252634649745No Hit
CATAGAAAAGTGTATTATGGGCCCAGGAGTTGACAAACGACAGCTTCTAT62492.8484301498741935No Hit
CATAGATGAGGGATGTAGGCTGTACATGTACACGGCAGGACTGTCTATGT56262.564453196222149No Hit
CATAGATGGGTCCCATTCTGTTTGCAGGATGAATGGGGACATCTATGTGT53452.43636728293768No Hit
CATAGAACATCTCCCTGTTCCTCCCACCTAAACTCCTGAGATCCTCTATG42261.9263027385771065No Hit
CATAGAGGTGAGCGGCTGTCTCCAGTAATCTATGTGTAGAACCATGTCGT40931.865678445100828No Hit
CATAGAAGGATAAAGGCGGAAGAGAGCCGTTTCCCCCATACTGTCTATGT37951.7298435619735257No Hit
CATAGAACTCTTACCCTTTTGCCAGGGCTGCATGGTGACTTTTCTATGTG31681.444043321299639No Hit
CATAGAGCAGTATCCCAGTCAGCTCCTGACAATACCGCGTCTATGTGTAG25681.1705502680231923No Hit
CATAGAGGCCATTCTTCTCATGCTGAAGGATAGTTGGGTGCTCTATGTGT21140.9636071910440142No Hit
CATAGACACTTGACCATGCCCTTGTTCGACACTTGAAAGCTCCCTGGATC21030.958593151733946No Hit
CATAGAAGGATAAAGGCGGAAGAGAGCATAATCTATAGGAGTTGACAAAC18990.8656055136199541No Hit
CATAGATCCATGGTGTGCAAGGACAATCACAGGGCTGCATGGTGACTTTT18640.8496517521788279No Hit
CATAGATGGGTCCCAAAGTAGGCAATTCTATGTGTAGAACCATGTCGTCA18440.8405353170696132No Hit
CATAGAAAAGTGTATTATGGGCCCAGGGCTGCATGGTGACTTTTCTATGT18320.8350654560040841No Hit
CATAGAAGGGGTGATGCTCTGATGGATCTCAGAGTTAGCTTGCCTCTATG17550.7999671808336069No Hit
CATAGAAAAGTGTATTATGGGCCCAGGAGGAGTTGACAAACGACAGCTTC17450.7954089632789993No Hit
CATAGAATAGCTGTGTTGAAGAACCTCATGCCAAGTAAGTCTACAAAGAA16320.7439011049119353No Hit
CATAGAACTGACCATACACTTGCCCTTCTGTCTAGGATGAGATAATTGCC16170.7370637785800241No Hit
CATAGATGAGGGATGTAGGCTGTACACGGCAGGGCTGCATGGTGACTTTT15980.7284031652262699No Hit
CATAGATCCATGGTGTGCAAGGACAAGGGCAGGGCTGCATGGTGACTTTT15080.6873792072348028No Hit
CATAGAGGTGAGCGGCTGTCTCCCAGTAATCTATGTGTAGAACCATGTCG15050.6860117419684206No Hit
CATAGAATAGCTGTGTTGAAGAACCTCAAGAAAGAAAACCCACAAAAGAC13740.6262990920030631No Hit
CATAGACTGGCTATTGTTTGGTTTGTACACTGGCTATCTTCTGCTGCACT13700.6244758049812201No Hit
CATAGATGGGTCCCATTCTGTTTGCACAGGACATTGGGGAATTCTATGTG12720.5798052729460671No Hit
CATAGAGAAGGAAAACTGGGCTGTGGGTAGAATCTTGAACTCAGGAGTCT11180.5096087226051125No Hit
CATAGATCCATGGTGTGCAAGGACAAGGGCAGGGATTCTATGTGTAGAAC11100.5059621485614265No Hit
CATAGAAGGATAAAGGCGGAAGAGAGCCATGCTGAAGTCCCATGATCTAT11040.5032272180286621No Hit
CATAGAACTGACCATACACTTGCCCTTTTGCCATGCTGAAGTCCCATGAT10860.4950224264303687No Hit
CATAGAACTCTTACCCTTTTGCCAAGTAAGTCTACAAAGAACCTCTATGT10790.49183167414214346No Hit
CATAGACACTTGACCATGCCCTTGTTCTAGACACTTGAAAGCTCCCTGGA10730.489096743609379No Hit
CATAGAGTTCCAGCAAAATGAAGGGGACACTGACGCCATGCTGAAGTCCC10260.46767312110272397No Hit
CATAGAGGGATGGTATCAGAGAAGGCTGTCTCTCCCTCTCTCACACATCT10040.4576450424825876No Hit
CATAGAACTAGTTCCAGCACCACCAGGGCTGCATGGTGACTTTTCTATGT10030.45718922072712687No Hit
CATAGATCCATGGTGTGCAAGGACAAATCCCAGTTAGGCTACTTGGGGGT10020.4567333989716661No Hit
CATAGATCCATGGTGTGCAAGGACAAGGACAGGGGTGTGGTGTTCTGTCT10020.4567333989716661No Hit
CATAGAACTGACCATACACTTGCCCTTCGTCTAGGATGAGATAATTGCCC9920.4521751814170587No Hit
CATAGACTGGTGTAATGTGTCATATACTTGGTAGGTGGCTTCTGGTGCAT9810.4471611421069905No Hit
CATAGAACCTCAAGCCGGAAAACCTTGTCGTCAGTGTTGCCAAGTAAGTC9490.4325748459322466No Hit
CATAGAAAAGGGAGGGATTACGGGTGTCCAAGGTCAAGGGCAGGGATTCT9390.4280166283776392No Hit
CATAGATGGGTCCCATTCTGTTTGCATAGATGGGTCCCAAAGTAGGCAAT9300.4239142325784925No Hit
CATAGAGCAGACAATGGATGATCAAGTGACACCAAAAGCACAAGTTCTAT9290.42345841082303176No Hit
CATAGACACTTGACCATGCCCTTGTTCTCATAGACACTTGAAAGCTCCCT9010.4106954016701309No Hit
CATAGAACTCTTACCCTTTTGCCAGGAGTTGACAAACGACAGCTTCTATG8680.3956532837399263No Hit
CATAGAAGGATAAAGGCGGAAGAGAGCATAATCTACAGAGTGGGAGTCAT8650.3942858184735441No Hit
CATAGATGGGTCCCATTCTGTTTGCAGAGACTTGGTCTCATAGATGGGTC8420.383801918097947No Hit
CATAGATGAAAACATTCCGAAGCTAGATCATACGGTAGGTGGCTTCTGGT8250.3760529482551143No Hit
CATAGACGTGTGGTTGGGATGTCAGCTCCTGACAATACCGCGTCTATGTG8180.3728621959668891No Hit
CATAGAACTAGTTCCAGCACCACCAGCTGCCAGGCTAGGCCTCCAAGGCT7930.3614666520803705No Hit
CATAGATTCTGTTTCTCTTCCCCCAAACCTGTGTGTGTAAGTGGGTCTAT7860.35827589979214525No Hit
CATAGAAGAGAGCATAATCTATAGAGTTGGAACCACCAAGGTCAAGGGCA7800.35554096925938083No Hit
CATAGAATAGCTGTGTTGAAGAACCTCACAGGGCTGCATGGTGACTTTTC7700.3509827517047734No Hit
CATAGAACTGACCATACACTTGCCCTTTTGCCAAGTAAGTCTACAAAGAA7690.3505269299493126No Hit
CATAGAACATCTCCCTGTTCCTCCCAGATGTCCTCTTCACCTCCCAGATC7630.34779199941654815No Hit
CATAGATGGGTCCCATTCTGTTTGCAGACTTGGTCTCATAGATGGGTCCC7610.34688035590562666No Hit
CATAGAACTGTCCCCTACTTTTCACCATCTCATAGAACTTTCCCACCGCC7460.3400430295737155No Hit
CATAGAGTTCCAGCAAAATGAAGGGGTGATGCTCTGAGGAAGTGACATGC7400.337308099040951No Hit
CATAGATGGGTCCCATTCTGTTTGCACTTGGTCTCATAGATGGGTCCCAA7190.3277358421762754No Hit
CATAGACTTGTTGCCGAGACTGGAGTATAGACACTTGAAAGCTCCCTGGA7170.3268241986653539No Hit
CATAGAGAAGGAAAACTGGGCTGTGGATCTCAGAGTTAGCTTGCCTCTAT6870.31314954600153155No Hit
CATAGACACTTGACCATGCCCTTGTTCTTGTCCCCTATTACTGAAAGTTC6710.30585639791415964No Hit
CATAGATTCTGTTTCTCTTCCCCCAAAGCAGCAAACAATTTCAGAAAGTC6580.29993071509316993No Hit
CATAGAACTGACCATACACTTGCCCTTCTATGGACAGGGGTGTGGTGTTC6480.29537249753856254No Hit
CATAGAAGGATAAAGGCGGAAGAGAGCGCCAGTCTCCCAGTAATCTATGT6460.29446085402764105No Hit
CATAGAGGCCATTCTTCTCATGCTGGGTCCCAAAGTAGGCAATTCTATGT6430.29309338876125884No Hit
CATAGACTCAGAACTGGCTCTAATTCCTGTCTCTCCCTCTCTCACACATC6280.28625606242934765No Hit
CATAGAAGGATAAAGGCGGAAGAGAGCGTATCCCAGTTAGGCTACTTGGG6170.2812420231192794No Hit
CATAGAAAAGTGTATTATGGGCCCAGGCAGCTCCTCTTCTCTTCCCTCTA6090.27759544907559347No Hit
CATAGATGGGTCCCATTCTGTTTGCATGGGTCCCAAAGTAGGCAATTCTA5830.2657440834336141No Hit
CATAGAACTGACCATACACTTGCCCTAGATGTGGTTGCATGGGGGTCTAT5820.2652882616781534No Hit
CATAGAGGGATGGTATCAGAGAAGGCTTACGGTTGCAGGATGAATGGGGA5810.2648324399226926No Hit
CATAGAACTGACCATACACTTGCCCTTCTATGGTCTAGGATGAGATAATT5680.25890675710170297No Hit
CATAGATGGGTCCCATTCTGTTTGCAGATGGGTCCCAAAGTAGGCAATTC5510.2511577872588703No Hit
CATAGATTCTGTTTCTCTTCCCCCAAGGTCAAGGGCAGGGATTCTATGTG5370.24477628268241985No Hit
CATAGACTGGTGTAATGTGTCATATACTTGGTACGGTAGGTGGCTTCTGG5280.24067388688327318No Hit
CATAGAACCAACCAATAATAATGTGCTTGTGACACCAAAAGCACAAGTTC5260.23976224337235166No Hit
CATAGATCCATGGTGTGCAAGGTCAAGGGCAGGGATTCTATGTGTAGAAC5240.2388505998614302No Hit
CATAGATCCATGGTGTGCAAGGACATTGGGGAATTCTATGTGTAGAACCA5220.23793895635050868No Hit
CATAGATCCATGGTGTGCAAGGACAGGGGTGTGGTGTTCTGTCTATGTGT5170.235659847573205No Hit
CATAGATCCATGGTGTGCACAGGACATTGGGGAATTCTATGTGTAGAACC5130.23383656055136198No Hit
CATAGAAGGAGCTGGGAGTTGAGATAATTGCCCCTGCTCACGATGGTATT5110.23292491704044052No Hit
CATAGAGCTGAGAAGGTAGGTAAAGGGGCTCATAGATGGGTCCCAAAGTA4880.22244101666484337No Hit
CATAGACCAGGACAATCACAGGGCACAGTTAGGCTACTTGGGGGTCTCTA4540.20694307697917808No Hit
CATAGATCCATGGTGTGCAAGGACAAGTGCACAGTTTGGAATGACTCTAT4390.2001057506472669No Hit
CATAGAACTCTTACCCTTTTGCCAGGTAAGTCTACAAAGAACCTCTATGT4210.1919009590489735No Hit
CATAGATCTACTCCTACAATCTTGGCAAAAGGGGCTAACTTCGCCTGCCT4180.19053349378259124No Hit
CATAGATTCTGTTTCTCTTCCCCCAAACTGTCAGGAGTTGACAAACGACA4060.18506363271706233No Hit
CATAGAGCAGACAATGGATGATCAAGTGATTCTATGTGTAGGTAGGTGGC3930.17913794989607262No Hit
CATAGATGAAAACATTCCGAAGCTAGATCAGGAGTTGACAAACGACAGCT3900.17777048462969042No Hit
CATAGATGGGTCCCATTCTGTTTGCACACTGCTCACGATGGTATTACATG3780.17230062356416148No Hit
CATAGACTGGTGTAATGTGTCATATACTTGGTCTAGGATGAGATAATTGC3670.1672865842540933No Hit
CATAGATGGGTCCCATTCTGTTTGCAGTCTCATAGATGGGTCCCAAAGTA3600.16409583196586808No Hit
CATAGATGAAAACATTCCGAAGCTAGATCAGTGTACCCAGAAGAGAAGTA3580.1631841884549466No Hit
CATAGAACTGTCCCCTACTTTTCACCATAGAACTTTCCCACCGCCCTTTT3520.1604492579221821No Hit
CATAGATGAGGGATGTAGGCTGTACACATGTACACGGCAGGACTGTCTAT3510.15999343616672138No Hit
CATAGAACTAGTTCCAGCACCACCAGTGTAGGAGTTGACAAACGACAGCT3490.1590817926557999No Hit
CATAGACACTTGACCATGCCCTTGTTCTATGTGTAGAACCAGGGCTGCAT3420.15589104036757467No Hit
CATAGACAGGGGCCAGAACTCCTAAGGTCTCATAGACAGGGGTGTGGTGT3380.15406775334573167No Hit
CATAGAAGAGAACCAAAAATGGACCCACCTAAACTCCTGAGATCCTCTAT3350.15270028807934946No Hit
CATAGACAATTTCAGTGCGGCCGATGCATGTCTAGATGTGGTTGCATGGG3300.15042117930204574No Hit
CATAGATGGGTCCCATTCTGTTTGCAGCAGAGACTTGGTCTCATAGATGG3280.14950953579112425No Hit
CATAGAGTTCCAGCAAAATGAAGGGGTGCTGCTCACGATGGTATTACATG3200.14586296174743826No Hit
CATAGAACTAGTTCCAGCACCACCAGATCTATGCCAAGGTCAAGGGCAGG3140.14312803121467382No Hit
CATAGAACATCTCCCTGTTCCTCCCAGTTAGGCTACTTGGGGGTCTCTAT3130.14267220945921305No Hit
CATAGAACTGTCCCCTACTTTTCACCAGAGACTTGGTCTCATAGAACTTT3020.13765817014914486No Hit
CATAGATCCATGGTGTGCAAGGACAAAGCAGCAAACAATTTCAGAAAGTC3020.13765817014914486No Hit
CATAGAATAGCTGTGTTGAAGAACCTCAAGCCGGAAAACCTGTGTGTGTA2980.1358348831273019No Hit
CATAGAGAAGCCAGGGAACAAAGTGGATCTCAGAGTTAGCTTGCCTCTAT2940.13401159610545893No Hit
CATAGAAGGAGCTGGGAGTTGAGATAGGAGTTGACAAACGACAGCTTCTA2920.13309995259453744No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA2890.1317324873281552No Hit
CATAGAACTCTTACCCTTTTGCCAGGGAACAGAGTGGGAGTCATTCATGA2690.1226160522189403No Hit
CATAGAGCTTCAGATTCCATAAGTCATCCTGACTGGAGTTCAGACGTGTC2580.11760201290887211TruSeq Adapter, Index 9 (95% over 24bp)
CATAGATTCCCCTCATACCACGCCTCAGTTCTATGTGTAGAACCATGTCG2560.11669036939795062No Hit
CATAGACAGGGGTGTGGTGTTCTGTCTATGTGTAGAACCATGTCGTCAGT2530.1153229041315684No Hit
CATAGACGTGTGGTTGGGATGTCAGCCTGCCCCTACTGGGGGGTGCCTCC2440.11122050833242168No Hit
CATAGAGAAGGAAAACTGGGCTGTGACACCAAAAGCACAAGTTCTATGTG2330.10620646902235349No Hit
CATAGAGGGATGGTATCAGAGAAGGCTAACTTCGCCTGCCTTTCTCTATG2300.10483900375597127No Hit
CATAGAGTTCCAGCAAAATGAAGGGGTACGGTAGCAGCTCCTCTTCTCTT2260.10301571673412828No Hit
CATAGACCAGGACAATCACAGGGCAGGGCTGCATGGTGACTTTTCTATGT2220.1011924297122853No Hit
CATAGAGCAGACAATGGATGATCAAGTGATAGGAGTTGACAAACGACAGC2220.1011924297122853No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)