FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54416
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG2064037.93002058218171No Hit
CATAGAGAATTTGCACACCACGGGACATGGATGAAGCTGGATCTATGAGA22394.1145986474566305No Hit
CATAGATGTCTCCCAGGCTAGAGTGAGCCCTTGTCCCTTTCTATGAGACC12092.2217730079388414No Hit
CATAGACCACACCCAAACACCACGTACACTGACGACATGGTTCTACACAT10671.9608203469567775No Hit
CATAGACTTGATGCCCAGGAAACCTCCTGCCCCTGCAGGCTGAAAGTGCC9981.834019406057042No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAATCAGGGTTGGTTCTATGAGACC8341.5326374595707146No Hit
CATAGATCATGGGACTTCAGCATGGTGGGTGCCTGATTGTTCTATGAGAC7261.3341664216406939No Hit
CATAGATTCTCCCTTGTGCTTTTGGTACACTGACGACATGGTTCTACACA6771.2441193766539254No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGAGAGGCTGTTTGAGATCT6141.1283446045280798No Hit
CATAGAGGGTGTTTTTCGGTGTTACAACCAATGCTCTTTCATCTATGAGA5110.939062040576301No Hit
CATAGAACAAGGCCAGAATTACCCTGGGAGAGGCTGTTTGAGATCTATGA4850.8912819758894442No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGCAACAGAACAACAACATCTATG4390.8067480152896206No Hit
CATAGAACAAGGCCAGAATTACCCTGGGTGAGAAATGATGATGGCTCTAT4320.7938841517200824No Hit
CATAGAGAATTTGCACACCACGGGACCATGCTCACACCTTGATCTATGAG4180.7681564245810055No Hit
CATAGAGCAAAAGAGAAACATTAAGCCATAGTACACTGACGACTCTTCAC3950.7258894442810938No Hit
CATAGAGGGTGTTTTTCGGTGTCACAACCAATGCTCTTTCATCTATGAGA3680.6762716847985887No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGGAACAGAACAACAACATCTATG3380.6211408409291385No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGTGAGAAATGATGATGGCT3370.6193031461334901No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCACTGACGACATGGTTCTACACAT3190.58622463981182No Hit
CATAGACCTTCCTGTTGGCCACACAGAATTAGCGGAGAGTGAGAACTCTA3190.58622463981182No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACCACGGGACATGGATGAAGC2980.547633049103205No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTCTATGAGACCAAGTCTCTGC2910.5347691855336666No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACATGGGCAAC2720.4998529844163481No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCCAGGCCTGAGATTGCTGCTAT2560.4704498676859747No Hit
CATAGACAGGAGGAGGAAGAAGAGGAATTCTTGCCAGGGATCTATGAGAC2350.4318582769773596No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGCAACAGAACAACAACATC2220.4079682446339312No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTGGGAGAGGCTGTTTGAGATC2180.40061746545133786No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGAGGAATTCTTGCCA2050.37672743310790946No Hit
CATAGAGAAGAGAAGGCGACAATGGCTAACCAGGGGGAAGAGAAGGGAGA1910.35099970596883273No Hit
CATAGACAGCTCACAGACTGCATACTGGGTGAGAAATGATGATGGCTCTA1740.3197588944428109No Hit
CATAGAGGGTGTTTTTCGGTGTCACACACTGACGACATGGTTCTACACAT1660.3050573360776242No Hit
CATAGAGAAGAGAAGGCGACAATGGCTGCTTTTCTCTTCTTGGCATCTAT1520.27932960893854747No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACCACGGGACCATGCTCACAC1510.27749191414289914No Hit
CATAGAACAATGTAATAAGCATCGGAGGACACTGACGACATGGTTCTACA1480.2719788297559541No Hit
CATAGACAGGAGGAGGAAGAAGAGGAAGAAGAAGAGAGCCTCTGTCCCTG1470.2701411349603058No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAGTGAGCCCTTGTCCCTTTCTATG1460.26830344016465746No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTCTATGAGGACTCTTCACTAT1450.26646574536900913No Hit
CATAGAACAATGTAATAAGCATCGGAGGAACACTGATCTGAGCAAGGATA1300.23890032343428405No Hit
CATAGAATCAAAATAGTACCCTTGCCTCTATGAGACCACTGGGAGAGGCT1290.23706262863863573No Hit
CATAGAGCAAAAGAGAAACATTAAGCCATAGTACACTGACGACATGGTTC1290.23706262863863573No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTCGCAGATTTGGACCTGCG1180.21684798588650395No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTTATAGTCAAGGACTTTTAAG1170.21501029109085562No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCCGCG1100.20214642752131728No Hit
CATAGAACAAGGCCAGAATTACCCTGCACTACAAGAGTTACCCAAAGCAT1060.19479564833872393No Hit
CATAGATTCTCCCTTGTGCTTTTGGTACACTGACGACATGGTGGGTGCCT1060.19479564833872393No Hit
CATAGACAGCTCACAGACTGCATACTACACTGACGCATGGTGGGTGCCTG1010.1856071743604822No Hit
CATAGATTCTCCCTTGTGCTTTTGGTACACTGATCTGAGCAAGGATAGCC1000.18376947956483386No Hit
CATAGATCATGGGACTTCAGCATGGCAGTGTTTGCAGATTTGGACCTGCG980.18009408997353718No Hit
CATAGACCACACCCAAACACCACGTAGTACTACGCAGCCATAAAAAATGA960.17641870038224053No Hit
CATAGAGAAGAGAAGGCGACAATGGCCGCTAAGCCGAGAAGATCTCTATG960.17641870038224053No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCGGATTTGGACCTGCG940.17274331079094382No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTCTGCAGATTTGGACCTGCG940.17274331079094382No Hit
CATAGATTAGTTCTTATTCTTGGTATGGCACACACTGGGCAACAGAACAA930.1709056159952955No Hit
CATAGATCATGGGACTTCAGCATGGTGGTGTTTGCAGATTTGGACCTGCG920.16906792119964717No Hit
CATAGACCCTTAGAGTTTCAGCCTCCCAAACTGCTGAGATTCCAGGCATG910.16723022640399882No Hit
CATAGAGCTATCAGTATACTCCCACCATGAGGAATTCTTGCCAGGGATCT890.16355483681270214No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGCTTGCAGATTTGGACCTGCG860.15804175242575713No Hit
CATAGAGGGTGTTTTTCGGTGTCACACTGACGACATGGTTCTACACATAG820.15069097324316377No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGGCCTGCG760.13966480446927373No Hit
CATAGACAGCTCACAGACTGCATACTACACTGACGACATGGTGGGTGCCT750.1378271096736254No Hit
CATAGATTAGTTCTTATTCTTGGTATGGCACACTGACGACATGGTTCTAC740.13598941487797706No Hit
CATAGATCATGGGACTTCAGCATGGCGGCGTTTGCAGATTTGGACCTGCG740.13598941487797706No Hit
CATAGATCTGTGGCTCCCGATAAATGTGCTCATACAGAATTAGATCTAGC730.13415172008232873No Hit
CATAGAGCTATCAGTATACTCCCACCATGATCTGAGCAAGGATAGCCCTC730.13415172008232873No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGACTTGGACCTGCG710.13047633049103205No Hit
CATAGATGTCTCCCAGGCTAGAGTGCGCCCTTGTCCCTTTCTATGAGACC690.12680094089973537No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGAGACAGCCGCTCACCTCTA680.12496324610408703No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAGAGTGAGCCCTTGTCCCTTTCTA660.12128785651279037No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGCTTTTCTCTTCTTGGCATCT630.11577477212584533No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGGTTTGGACCTGCG630.11577477212584533No Hit
CATAGAGAATTTGCACACCACGGGACATGGATGAAGCTGGACCTATGAGA620.113937077330197No Hit
CATAGAACCCAAAGAGTGAGCCCTTGTCCCTTTCTATGAGACCAAGTCTC610.11209938253454867No Hit
CATAGAACCCAAAGAGTGGGCAACAGAACAACAACATCTATGAGACCAAG590.10842399294325197No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTCGGACCTGCG590.10842399294325197No Hit
CATAGAGGGTGTTTTTCGGTGTCACAACCCCCAGTATTTCATTATTAAAA580.10658629814760363No Hit
CATAGAACAAGGCCAGAATTACCCTGCACTGACGACATGGTTCTACACAT570.10474860335195531No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATCTGGACCTGCG560.10291090855630697No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAGAGAGAGCTGTTCACTTTTCTCG560.10291090855630697No Hit
CATAGATCTGTGGCTCCCGATAAATGTCACATAGATCTGTGCTCATACAG550.10107321376065864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)