FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53000
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG1557429.38490566037736No Hit
CATAGAGAGATTTTGGGCTGAGACGATAATGCCGCATATCTACAACTATC37827.135849056603774No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTTTCTAGATATACAACAA24714.662264150943396No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGATAGGGTT17543.309433962264151No Hit
CATAGAACTTCACAAGGGCTTTAGGGGCTACACATGGCTATGCAATCTAT15202.8679245283018866No Hit
CATAGACCTGGGCTTAGGGGCTACACATGGCTATGCAATCTATGAGACCA14292.6962264150943396No Hit
CATAGACCTGGGCTTAGGGGCTATACATGGCTATGCAATCTATGAGACCA13432.5339622641509436No Hit
CATAGACACCATGCCTGGCTAATTTTTTGCGTTTTTAGTAGAGATGGGGT11002.0754716981132075No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTTTCTAGATATACAATCA8121.532075471698113No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGGGCAAGCAATGACAG7621.4377358490566037No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTTTCTAGATATACACTCA6431.2132075471698114No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTGGTGCACATGCC4970.9377358490566038No Hit
CATAGATGCCCTTTTTGTGTACTTCCACACTGACTGATACAGGAAGGCCT4900.9245283018867925No Hit
CATAGAGCCAAGGAAAGTTGAATGGAGACAGCCGCTCACCTCTATGAGAC4660.879245283018868No Hit
CATAGACCTGGGCTTAGGGGCTATACGTGAATGTGGATCAGAACTTCTAT4200.7924528301886792No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGGGGAAAGGATTCTAT4010.7566037735849056No Hit
CATAGAAACCCCAAAGGCTATGTTCAAAATGGTCAAACAGGAGGTCTATG3910.7377358490566038No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTTAGCATGTCTATGAGAC3780.7132075471698114No Hit
CATAGAGCTGGCTTCCTAACTTCCAATAAATGACCTTCTCTTCTGTCTCT3270.6169811320754717No Hit
CATAGATGAAAGCCTGTTGAATTTATGACTGATACAGGAAGGCCTTTCTA3170.5981132075471698No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATATCCCCAGAAATGTTAGCA2630.49622641509433957No Hit
CATAGATGCCCTTTTTGTGTACTTCCACTGACGACATGTTCTACACATAG2620.49433962264150944No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAACCTGTCTCTACT2570.4849056603773585No Hit
CATAGATGCCCTTTTTGTGTACTTCCATTCTACACATAGATGCCCTTTTT2360.44528301886792454No Hit
CATAGACACCATGCCTGGCTAATTTTAAAATTAGTACCTATAATCTCAGC2210.4169811320754717No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTATCCCCAGAAATGTTAGCATGT2190.41320754716981134No Hit
CATAGAACCTCTTTTGGGGCAAAGTTCCAATAATCTGTGAGTTGCTGATG2090.3943396226415094No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTTATCCCCAGAAATGTTA1900.3584905660377358No Hit
CATAGATCCTCTCATTGTCCAGTTTGGAGACAGCCGCTCACCTCTATGAG1890.3566037735849057No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGTGGTGTGGTG1880.35471698113207545No Hit
CATAGACAGGGAGGCGTATAAGTGATGCGTATACGTGAATGTGGATCAGA1830.34528301886792456No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTACACTGACTGATACAGGAAGGC1800.33962264150943394No Hit
CATAGACCTTTGGGTTCATATCATCTGGAGACAGCCGCTCACCTCTATGA1780.33584905660377357No Hit
CATAGACCTTTGGGTTCATATCATCTGGCTACACATGGCTATGCAATCTA1760.3320754716981132No Hit
CATAGAGCTGGCTTCCTAACTTCCAATGGGCAAGCAATGACAGTCTATGA1680.3169811320754717No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTCGGACCTGCG1680.3169811320754717No Hit
CATAGACACCATGCCTGGCTAATTTTTTGTATTTTTAGTAGAGATGGGGT1670.3150943396226415No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTTCCTTCTGCTGGCTATTCTATG1640.30943396226415093No Hit
CATAGATCCCATCCAATAATAATGAACAAGCAATGGGGAAAGGATTCTAT1560.2943396226415094No Hit
CATAGATGAAAGCCTGTTGAATTTATGAGACCAAGTCTCTGCTACCGTAA1540.29056603773584905No Hit
CATAGACACCATGCCTGGCTAATTTTTAAAAAGTAGCTGGGCATGGTGGC1510.2849056603773585No Hit
CATAGATTGGAACTTTGCCAGCCTTAACACTGACTGATACAGGAAGGCCT1510.2849056603773585No Hit
CATAGACACCATGCCTGGCTAATTTTTTGTATTAATCCCAGCTACTCAGG1500.2830188679245283No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTACCATGTTTTCATCCTG1460.27547169811320754No Hit
CATAGATGCCCTTTTTGTGTACTTCCACGACATGGTTCTACACATAGATG1450.27358490566037735No Hit
CATAGACCAATAAAAGTTGATGGGATGAACCATGTTGGAGACAGCCGCTC1440.27169811320754716No Hit
CATAGACAATTAAATTTTCTTAAGGGGTTGACTGATACAGGAAGGCCTTT1410.2660377358490566No Hit
CATAGAACTTCACAAGGGCTTTAGGGCTACACATGGCTATGCAATCTATG1360.25660377358490566No Hit
CATAGATCATGGGACTTCAGCATGGAGACAGCCGCTCACCTCTATGAGAC1290.24339622641509434No Hit
CATAGACAGGGAGGCGTATAAGTGATGATACTGACTGATACAGGAAGGCC1210.2283018867924528No Hit
CATAGATGTATACAAGCTGTCACAGACAACAAGCAATGGGGAAAGGATTC1150.21698113207547168No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGACAGTCTATGAGACC1100.20754716981132076No Hit
CATAGACAGGGAGGCGTATAAGTGATGACACTGACTGATACAGGAAGGCC1090.20566037735849055No Hit
CATAGACACCATGCCTGGCTAATTTTTAAATATTTTGTAGAGACCGGGTT1090.20566037735849055No Hit
CATAGATCCCATCCAATAATAATGAGCAGAATTACCTCTGGATGGTCTAT1020.19245283018867926No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCCCGTCTCTA1010.19056603773584907No Hit
CATAGATTGGAACTTTGCCAGCCTTATAAATGACCTTCTCTTCTGTCTCT960.1811320754716981No Hit
CATAGATCCCATCCAATAATAATGAGCAATGGGCAAGCAATGACAGTCTA940.17735849056603772No Hit
CATAGACAGGGAGGCGTATAAGTGATGTACACATACTCAGGAGGCTGAGG900.16981132075471697No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTTGTAGAGACAGAGTT880.1660377358490566No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCGGATTTGGACCTGCG870.1641509433962264No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCCCATCTCTA850.16037735849056603No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTCGCAGATTTGGACCTGCG840.15849056603773584No Hit
CATAGATCCCATCCAATAATAATGAGCATCCTTTCCCTCACTCTCTCCAT830.15660377358490565No Hit
CATAGATCCTCTCATTGTCCAGTTTGGACACTGACTGATACAGGAAGGCC820.15471698113207547No Hit
CATAGATGAAAGCCTGTTGAATTTATGAGATGTGCTTCTTTCAAAAGTCT790.1490566037735849No Hit
CATAGACACCATGCCTGGCTAATTTTGTATTTTTAGTAGAGACAGGGTTT790.1490566037735849No Hit
CATAGACAGAAACCACCCAGACAACCTGATTGGCCATTTGAACTCTATGA770.14528301886792452No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCCGCG770.14528301886792452No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTTAGCATGTATCCCCAGA760.14339622641509434No Hit
CATAGATCATGGGACTTCAGCATGGCTATACAAGCAATGGGGAAAGGATT760.14339622641509434No Hit
CATAGATGCCCTTTTTGTGTACTTCCACACTGACGACATGGTTCTACACA730.13773584905660377No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTCTCTAAATATACACTCA730.13773584905660377No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTACACTGACGACATGGTTCCTCA680.12830188679245283No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGGCCTGCG630.11886792452830189No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTCTGCAGATTTGGACCTGCG600.11320754716981132No Hit
CATAGACACCATGCCTGGCTAATTTTTTTGTGTTTTTGGTAGAGATGGGG580.10943396226415095No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGACAGGGTT580.10943396226415095No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGACTTGGACCTGCG540.1018867924528302No Hit
CATAGATTGGAACTTTGCCAGCCTTATACGGTAGCAGAATTACCTCTGGA540.1018867924528302No Hit
CATAGACCTTTGGGTTCATATCATCTGGACACTGATCTATCCAGGAATTT540.1018867924528302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)