FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59263
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG2627444.33457637986602No Hit
CATAGACTCAAGCAATCCTCCCACCTCAGCCTCCCAAGTAGCCAGGACTA16842.841570625854243No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCGTGGAGGAAAGGAGGTCTATGAG15782.662706916625888No Hit
CATAGACTCAAGCAATCCTCCCACCTCAGCCTCCCAAATAGCCAGGACTA14762.4905927813306783No Hit
CATAGATCATGCAAAGAACACAGGCCACACTGACGACATGGTTCTACACA11121.8763815534144406No Hit
CATAGAGCTTCTGATCACTTTCGCCCCACCCTTATGATCGTATCTATGAG11091.8713193729645816No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCAGATTTGGACCTGCGAGCGGGTTC8231.3887248367446805No Hit
CATAGAACCAGAGTAATGAGGGATGCTCAGGTCTCCAATGGTGTCTATGA8081.363413934495385No Hit
CATAGAACTTGTAATCATTCCCTACTTCCCACACTCATGCAAAGCTGGTC6531.101867944585998No Hit
CATAGATGTGGCTATGCAGACTGAGCAGAGTTGGGTGTGTCTATGAGACC5800.9786882203060931No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATGAGACCAAGTCTCTG5280.890943759175202No Hit
CATAGAACCAGAGTAATGAGGGATGCTCTCTCCAGAACCCACCATCTATG5140.8673202504091929No Hit
CATAGATCATGCAAAGAACACAGGCCACACTCATGCAAAGCTGGTCTATG4760.803199298044311No Hit
CATAGAACTTTGTGCCCCCAGTGTCTTAGGTAGAGGAGTTTCAAACATCT4530.7643892479287244No Hit
CATAGAAGATCCCATAGCAGCTTTGCTACCGTAAGTTTTTCCAAATATAA3930.6631456389315424No Hit
CATAGATGTGGCTATGCAGACTGAGTAGAGTTGGGTGTGTCTATGAGACC2540.4285979447547374No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATGAGACCAAGTCTTCT2400.40497443598872823No Hit
CATAGAAGATCCCATAGCAGCTTTGCTACCGTAGTTTTTCCAAATATAAG2270.3830383207060054No Hit
CATAGATGTGCCTCTCCAACATTCATAGTAGCATGTTGAGATGAATAATT2100.3543526314901372No Hit
CATAGATCACCTACACAGACAGCTTTCCACACGACGACATGGTTCTACAC2060.3476030575569917No Hit
CATAGATCATGCAAAGAACACAGGCAGATCATGAGGTCAGGTCTATGAGA1770.29866864654168707No Hit
CATAGAGCTTCTGATCACTTTCGCCCCACTGACGACATGGTTCTACACAT1730.29191907260854155No Hit
CATAGAAGAATTGCTTGAATCCGGGAGGCAGAGGTTGCAGTGAGCTGAGA1660.28010731822553697No Hit
CATAGACTCAAGCAATCCTCCCACCTCAGCTTCCCTAAGTACCAGGATTA1620.27335774429239157No Hit
CATAGAGGGGCATGCTGGATAAATCTATGACCATGGGTCCTTTTCACATC1570.2649207768759597No Hit
CATAGACCCTTCTCCATGCCACATCATCTATGAGACCAAGTCTCTGCTAC1500.25310902249295514No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCGTGGAGGAAAGCAATCACCGCCA1450.2446720550765233No Hit
CATAGAAGATGGTCTGCTACACTGGTACACTGACGCCATGGGTCCTTTTC1440.24298466159323692No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTCGCAGATTTGGACCTGCG1380.2328603006935187No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCCGCG1340.22611072676037325No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCTCTGCTACCGTAGGCTAGTC1320.2227359397938005No Hit
CATAGATGTGGCTATGCAGACGGAGTGAGGGCAGTAAGTCTATGAGACCA1310.22104854631051413No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCTTCTTATAGCCAGTGTCTCCC1300.21936115282722776No Hit
CATAGAGTCTCGCTAATAGTTTTACCTCAAACGACATGGTTCTACACATA1290.21767375934394143No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGGCCTGCG1260.21261157889408233No Hit
CATAGACCCTTCTCCATGCCACACTCATGCAAAGCTGGTCTATGAGACCA1220.20586200496093685No Hit
CATAGAAGGAGGGGAAACTGAGGCCAGACGCCGTGGCTCACACCTGTAAT1190.20079982451107775No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTCTGCAGATTTGGACCTGCG1190.20079982451107775No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCGTGGAGGGAAGGAAGGGCAAACT1160.19573764406121863No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGGGAGGGAAGGAAGGGCAAACTC1150.19405025057793227No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCCCTGCTAGTGCAGCTATATT1140.1923628570946459No Hit
CATAGAAGGAGGGGAAACTGAGGCCAGGCGCGGTGGCTCATGCCTGTAAT1120.18898807012807317No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGCTTGCAGATTTGGACCTGCG1080.1822384961949277No Hit
CATAGAACACAGATCTGCTTGGAGGTACACTGACGGAGTGAGGGCAGTAA1060.17886370922835496No Hit
CATAGAACACAGATCTGCTTGGAGGGAAGGAAGGGCAAACTCTATGAGAC1030.17380152877849586No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGGGCTAGTCCGTCACCCCTAATC1010.17042674181192313No Hit
CATAGATGTTAGGTGCTCATTTTAAAACATCATCCCATGGGTCCTTTTCA1000.16873934832863674No Hit
CATAGATGTGCCTCTCCAACATTCATTTCCCCTCTCTCTGAATAACTCTA1000.16873934832863674No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCGGATTTGGACCTGCG960.16198977439549128No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGGTTTGGACCTGCG960.16198977439549128No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATTCTTATAGCCAGTGT940.15861498742891855No Hit
CATAGATCATGGGACTTCAGCATGGCGGCGTTTGCAGATTTGGACCTGCG940.15861498742891855No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTTCTTATAGCCAGTGTCT930.15692759394563216No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCTCTGGAGGGAAGGAAGGGCA920.15524020046234582No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCA900.1518654134957731No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTCGGACCTGCG840.14174105259605488No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCATGTCTTCTGTGTTTGGTCTATG830.14005365911276849No Hit
CATAGATGTGGCTATGCAGACTGAGTGAGGGCAGTAAGTCTATGAGACCA830.14005365911276849No Hit
CATAGAAGGAGGGGAAACTGAGGCCATGGGTCCTTTTCACATCATCTATG820.13836626562948212No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATGAGACCAAGTTCTTA820.13836626562948212No Hit
CATAGAAGGAGGGGAAACTGAGGCCAGATAGGCTAAGTTAGTGGCCCAAT810.13667887214619578No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATCTGGACCTGCG800.13499147866290942No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGACTTGGACCTGCG770.1299292982130503No Hit
CATAGAGAGGTCGCCCAGGTATTTCTTAGGTAGAGGAGTTTCAAACATCT770.1299292982130503No Hit
CATAGAAGATGGTCTGCTACACTGGTTACGGGGGCAGATTAACAGGTCAT750.12655451124647757No Hit
CATAGAAGGAGGGGAAACTGAGGCCACTGACGACATGGTTCTACACATAG730.12317972427990484No Hit
CATAGAACTTTGTGCCCCCAGTGTCTTCTTATAGCCAGTGTCTCCCCTCT700.11811754383004573No Hit
CATAGACCCTTCTCCATGCCACATCAGGGATTATCAGTAGTGCTCACATC700.11811754383004573No Hit
CATAGATCTAGTACTGTAATTGTAGTGTGCAGGGATTATCAGTAGTGCTC690.11643015034675935No Hit
CATAGAGCTTCTGATCACTTTCGCCCTCTCTCCAGAACCCACCATCTATG690.11643015034675935No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTTCTTATAGCCAGTGTCTCC680.11474275686347299No Hit
CATAGATCATGGGACTTCAGCATGGTGGTGTTTGCAGATTTGGACCTGCG680.11474275686347299No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCTCTGCTACAGGAGGGAAGGA660.11136796989690025No Hit
CATAGAAGAATTGCTTGAATCCGGGAGGGAAGGAAGGGCAAACTCTATGA660.11136796989690025No Hit
CATAGATGTGCCTCTCCAACATTCATCTATGAGACCCATGGGTCCTTTTC640.10799318293032753No Hit
CATAGAGGTCGCAGCTACCCAGAAAGGCTGGGCGCAGTGGCTCAAGCCTG600.10124360899718206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)