FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97483
Sequences flagged as poor quality0
Sequence length150
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG3223533.06730404275618No Hit
CATAGAGGTAAACAAAATAAGACATTACATAGGAGTGACATGGTATATTT95349.780166798313553No Hit
CATAGAAGAGTGAGACTTGAGATGGGATCAGACTTTATGTGCAATCTATG51085.2398879804683896No Hit
CATAGAAAATAATGTGGCCGGGCATGGTGGCTCACACCTGTAATCACAGC32843.3687925074115483No Hit
CATAGACACATGTAGGATGGGGTGGCTGGGCATTGCTCTTGACATCTCTA22622.320404583363253No Hit
CATAGACACATGTAGGATGGGGTGGAGGATGATCAATGCTGCTTCTATGA20532.10600822707549No Hit
CATAGAGGGTGCTCCTGTGCTGGGTAGTGGTAGTCTATGAGACCAAGTCT20142.0660012515002615No Hit
CATAGACTTCAGCCCTCACAAGCCTTACCTCAAATGCCAGGCTCTATGAG17331.7777458633813075No Hit
CATAGAGGTAAACAAAATAAGACATTACATAGGAGTTGGTCTCTGACTGT11181.1468666331565502No Hit
CATAGAACTTCAATGCTGTGTTAAAATGCTGGTATATTTTCTAAAGCCTG10851.1130145769005877No Hit
CATAGAGGGTGCTCCTGTACTGGGTAGTGGTAGTCTATGAGACCAAGTCT9180.9417026558476862No Hit
CATAGAACTTCAATGCTGTGTTAAAATGCTGTGCTGGGTAGTGGTAGTCT8890.9119538791379009No Hit
CATAGAGGGTGCTCCTGTACTGGGTGGTGGTAGTCTATGAGACCAAGTCT7730.7929587722987599No Hit
CATAGAAAGTCTGGACCAAGTGCCTCACTGGAGCTTCCGCAGAGCCCCCT7390.7580808961562528No Hit
CATAGAGGTAAACAAAATAAGACATTACATAGGAGTACACTGACGACATG7330.751925976836987No Hit
CATAGACACATGTAGGATGGGGTGGCTCACACCTGTAGTAATAGCACTTT6330.6493439881825549No Hit
CATAGAGGGTGCTCCTGTACTGGGTGGAGGATGATCAATGCTGCTTCTAT6120.6278017705651242No Hit
CATAGAACTTCAATGCTGTGTTAAAATGCTCTCTGACTGTGTTAATTTAA6040.6195952114727696No Hit
CATAGAACTCCATCATAAGTCAAAGAGCAATGCCATGATTGTAAGTTTCT5840.5990788137418832No Hit
CATAGAGCTATCTGGCAAAAACCCATCCTCAGACAGGATGCGCAGATGGT5170.5303488813434137No Hit
CATAGACTTCAGCCCTCACAAGCCTTGGCTGTCATGATTATCACCTTCTA4540.46572222849112155No Hit
CATAGACACATGTAGGATGGGGTGGCCTCTCCTTCACTTTCTGCCATGAT4510.4626447688314886No Hit
CATAGAAAATAATGTGGCCGGGCATGGTGGCTCACGCCTATAATTCCAGC4240.4349476318947919No Hit
CATAGACACATGTAGGATGGGGTGGCTCACACCTGTAATCACAGCACTTT4110.4216119733697158No Hit
CATAGAAAATAATGTGGCCGGGCATGGTGGCTCACGCCTGTAATCCCAGC4040.41443123416390554No Hit
CATAGACTTCAGCCCTCACAAGCCTTGGGCATTGCTCTTGACATCTCTAT3700.3795533580213986No Hit
CATAGAGGGTGCTCCTGTACTGGGTGGTATATTTTCTAAAGCCTGGGATC3520.36108860006360083No Hit
CATAGAGGTGTCCTTTCCCCACTTTAACACTGCCATCACAGTTCATTGTA3350.34364966199234737No Hit
CATAGAAAATAATGTGGCCGGGCATGGTGGCTCACACCTGTAATCCCAGC3150.323133264261461No Hit
CATAGACACATGTAGGATGGGGTGGCTCACACCTGTAATCCCAGCACTTT3140.3221074443749167No Hit
CATAGAAAATAATGTGGCCGGGCATGGTGGTTCACTCCTGTAATCCCAGC3100.31800416482873933No Hit
CATAGACACATGTAGGATGGGGTGGCTCACACCTATTATCCTAGCACTTT3060.3139008852825621No Hit
CATAGACACATGTAGGATGGGGTGGCTTCCCTTACACCTTCTGCCATGAT2710.27799718925351086No Hit
CATAGAGCATTACCCTCAAACTATCCCTACACTGACGACATGGTTCTACA2690.2759455494804222No Hit
CATAGAGGGTGCTCCTGTACTGGGTGGTGGTATATTTTCTAAAGCCTGGG2590.265687350614979No Hit
CATAGAAAGTCTGGACCAAGTGCCTCTCTGACTGTGTTAATTTAAAAGAA2440.2503000523168142No Hit
CATAGACTTCAGCCCTCACAAGCCTTGGTATATTTTCTAAAGCCTGGGAT2430.2492742324302699No Hit
CATAGAGGTGTCCTTTCCCCACTTTACACTGACGACATGGTTCTACACAT2300.2359385739051937No Hit
CATAGAAAATAATGTGGCCGGGCATGGTAGCTCATGCCTGTAATCCCAGT2190.2246545551532062No Hit
CATAGAACCAAAAATGGCTAGGCATGGGCATTGCTCTTGACATCTCTATG2050.21029307674158573No Hit
CATAGAGGTAAACAAAATAAGACATTACATAGGAGTTCCATGTGTGTGCG2040.2092672568550414No Hit
CATAGAAAATAATGTGGCTGGGCATTGCTCTTGACATCTCTATGAGACCA1990.20413815742231978No Hit
CATAGAGCTATCTGGCAAAAACCCATCTAGACCTTACCTCAAATGCCAGG1880.19285413867033224No Hit
CATAGAAAATAATGTGGCCGGGCATTGCTCTTGACATCTCTATGAGACCA1830.18772503923761066No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCCGCG1750.1795184801452561No Hit
CATAGAACCAAAAATGGCTAGGCATGTGGCTCACACCTGTAATCACAGCA1710.1754152005990788No Hit
CATAGAACTTCAATGCTGTGTTAAAATGCTGGAGGATGATCAATGCTGCT1580.16207954207400266No Hit
CATAGACTTCAGCCCTCACAAGCCTTGGAGACAGCCGCTCACCTCTATGA1550.15900208241436967No Hit
CATAGATGAAAGACAAGCAGGGAAGCCAGACAGGATGCGCAGATGGTCTA1520.15592462275473673No Hit
CATAGAGGGTGCTCCTGTACTGGGTGGTGGCAGACAGGATGCGCAGATGG1500.1538729829816481No Hit
CATAGACACATGTAGGATGGGGTGGCTCATGCCTGTAACCCTAGCACTTT1480.15182134320855944No Hit
CATAGAGGGTGCTCCTGTACTGGGTGGAGACAGCCGCTCACCTCTATGAG1480.15182134320855944No Hit
CATAGAAAATAATGTGGCCGGGCATGGTGGCTCATGCCTGTAATCCCAGC1450.14874388354892648No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCGGATTTGGACCTGCG1360.1395115045700276No Hit
CATAGACACATGTAGGATGGGGTGGCTCCTGTGCTGGGTAGTGGTAGTCT1340.13745986479693897No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTCGCAGATTTGGACCTGCG1340.13745986479693897No Hit
CATAGAACTTCAATGCTGTGTTAAAATGCTACCGTATGGAGGATGATCAA1340.13745986479693897No Hit
CATAGAACCAAAAATGGCTAGGCATGTTGGCTCACACCTGTAATCACAGC1290.13233076536421734No Hit
CATAGACACATGTAGGATGGGGTGGCTCACGTGTGTAATCTCAGCACTTT1290.13233076536421734No Hit
AATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG1280.13130494547767302No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTCTGCAGATTTGGACCTGCG1220.1251500261584071No Hit
CATAGAAAATAATGTGGCCGGGCATGGTGGCTCATGCCTATAATTCCAGC1190.12207256649877415No Hit
CATAGAACTCCATCATAAGTCAAAGAGCAGACAGGATGCGCAGATGGTCT1170.12002092672568551No Hit
CATAGATGAAAGACAAGCAGGGAAGCACACTGACGACATGGAGGATGATC1160.11899510683914118No Hit
CATAGAAGCATGGAAGACAAACAGCATGGCGGTGTTTGCAGATTTGGACC1150.11796928695259686No Hit
CATAGAACTCCATCATAAGTCAAAGAGCAATGCCATCACAGTTCATTGTA1130.11591764717950823No Hit
CATAGACACATGTAGGATGGGGTGGCTCATGCCTGTAATCCTAACACTTT1110.11386600740641958No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTCGGACCTGCG1110.11386600740641958No Hit
CATAGACTTCAGCCCTCACAAGCCTTGGGATCTGGTATATTTTCTAAAGC1090.11181436763333095No Hit
CATAGAAAATAATGTGGCCGGGCATGGTGGCTCACACTTGTAATCCTAGC1080.1107885477467866No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGACTTGGACCTGCG1070.1097627278602423No Hit
CATAGAAAATAATGTGGCCGGGCATGGTGGCTCACACCTGTAATACCAGC1070.1097627278602423No Hit
CATAGACTTCAGCCCTCACAAGCCTTTGCTACCGTAGGTCAAGGTGGGAG1070.1097627278602423No Hit
CATAGAAAATAATGTGGCCGGGCATGGTGGCTCGCTTCTGTAATCTCAGC1060.10873690797369798No Hit
CATAGAGGGTGCTCCTGTACTGGGTGGTGGTAGCAGACAGGATGCGCAGA1050.10771108808715366No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGCTTGCAGATTTGGACCTGCG1020.1046336284275207No Hit
CATAGAGCCACCATGTACTTCTATATAGCTTACACTGACGACATGGTATA1010.10360780854097638No Hit
CATAGAAAATAATGTGGCCGGGCATGGTGGCTTACTCCTGTAATTCCAGC1010.10360780854097638No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGGCCTGCG990.10155616876788773No Hit
CATAGAAAGTCTGGACCAAGTGCCTCCTGTGCTGGGTAGTGGTAGTCTAT980.1005303488813434No Hit
CATAGAAAATAATGTGGCCGGGCATGGTGGTGTGTGCCTGTAGTCCCAGC980.1005303488813434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)