FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97483
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA3240533.24169342346871No Hit
CATAGATCCCAGGCTTTAGAAAATATACCATGTCACTCCTATGTAATGTC94749.718617605120892No Hit
CATAGATTGCACATAAAGTCTGATCCCATCTCAAGTCTCACTCTTCTATG51325.264507657745453No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGTGATTACAGGTG40454.149441441071777No Hit
CATAGACTACCACTACCCAGCACAGGAGCACCCTCTATGTGTAGAACCAT22472.3050172850650883No Hit
CATAGAGATGTCAAGAGCAATGCCCAGCCACCCCATCCTACATGTGTCTA22272.284500887334202No Hit
CATAGAAGCAGCATTGATCATCCTCCACCCCATCCTACATGTGTCTATGT20412.0936983884369584No Hit
CATAGAGCCTGGCATTTGAGGTAAGGCTTGTGAGGGCTGAAGTCTATGTG17051.7490229065580665No Hit
CATAGACTGTTCTTTTAAATTAACACAGTCAGAGACCAACTCCTATGTAA11511.1807186894125128No Hit
CATAGATCCCAGGCTTTAGAAAATATACCAGCATTTTAACACAGCATTGA10781.1058338376947776No Hit
CATAGACTACCACTACCCAGCACAGCATTTTAACACAGCATTGAAGTTCT9120.9355477365284203No Hit
CATAGACTACCACTACCCAGTACAGGAGCACCCTCTATGTGTAGAACCAT9080.9314444569822431No Hit
CATAGACTACCACCACCCAGTACAGGAGCACCCTCTATGTGTAGAACCAT7400.7591067160427972No Hit
CATAGAGCCTGGCATTTGAGGTAAGGAACACAGGTGGATTTGGAAGCTGC7130.7314095791061005No Hit
CATAGATCCCAGGCTTTAGAAAATATACCATGTCGTCAGTGTACTCCTAT6900.7078157217155812No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTATTACTACAGGTG6350.6513956279556435No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGTG6340.6503698080690993No Hit
CATAGACTGTTCTTTTAAATTAACACAGTCAGAGAGCATTTTAACACAGC6110.6267759506785798No Hit
CATAGAAGCAGCATTGATCATCCTCCACCCAGTACAGGAGCACCCTCTAT6040.6195952114727696No Hit
CATAGACTCAAGAAACTTACAATCATGGCATTGCTCTTTGACTTATGATG5730.5877947949898957No Hit
CATAGACCATCTGCGCATCCTGTCTGAGGATGGGTTTTTGCCAGATAGCT5200.5334263410030466No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAGAGTGCTGGAATTATAGGCG4780.49034190576818526No Hit
CATAGAAGGTGATAATCATGACAGCCAAGGCTTGTGAGGGCTGAAGTCTA4470.4585414892853113No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAAGTGAAGGAGAGGCCAC4350.4462316506467795No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGCG4180.428792712575526No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGAG3960.4062246750715509No Hit
CATAGAGATGTCAAGAGCAATGCCCAAGGCTTGTGAGGGCTGAAGTCTAT3700.3795533580213986No Hit
CATAGATCCCAGGCTTTAGAAAATATACCACCCAGTACAGGAGCACCCTC3520.36108860006360083No Hit
CATAGAGAATACAATGAACTGTGATGGCAGTGTTAAAGTGGGGAAAGGAC3210.3292881835807269No Hit
CATAGACCATCCTCCCACCTTGACCTTCCAAAGTGCTAGGATAATAGGTG2930.3005652267574859No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGCA2730.2800488290265995No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAGGTGTAAGGGAAGCCAC2680.27491972959387795No Hit
CATAGATCCCAGGCTTTAGAAAATATACCACCACCCAGTACAGGAGCACC2600.2667131705015233No Hit
CATAGATCCCAGGCTTTAGAAAATATACCAAGGCTTGTGAGGGCTGAAGT2440.2503000523168142No Hit
CATAGACTGTTCTTTTAAATTAACACAGTCAGAGAGGCACTTGGTCCAGA2430.2492742324302699No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAACAGGTACTGGGATTACAGG2420.24824841254372557No Hit
CATAGACCATCTGCGCATCCTGTCTGCTCTATGTGTAGAACCATGTCGTC2220.22773201481283917No Hit
CATAGAACATCGCACACACATGGAACTCCTATGTAATGTCTTATTTTGTT2080.21337053640121867No Hit
CATAGAGATGTCAAGAGCAATGCCCAGCCACATTATTTTCTATGTGTAGA2070.21234471651467435No Hit
CATAGAGATGTCAAGAGCAATGCCCATGCCTAGCCATTTTTGGTTCTATG2020.20721561708195277No Hit
CATAGAGCCTGGCATTTGAGGTAAGGTCTAGATGGGTTTTTGCCAGATAG1820.18669921935106634No Hit
CATAGAGATGTCAAGAGCAATGCCCGGCCACATTATTTTCTATGTGTAGA1800.1846475795779777No Hit
CATAGAAGCAGCATTGATCATCCTCCAGCATTTTAACACAGCATTGAAGT1600.1641311818470913No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGGCTTGTGAGGGCTGAAGTCTATGT1580.16207954207400266No Hit
CATAGACCATCTGCGCATCCTGTCTGGCTTCCCTGCTTGTCTTTCATCTA1560.160027902300914No Hit
CATAGACCATCTGCGCATCCTGTCTGCCACCACCCAGTACAGGAGCACCC1540.15797626252782537No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA1530.15695044264128105No Hit
CATAGACCATCCTCCCACCTTGACCTCTCAAAGTGCTAGGGTTACAGGCA1490.15284716309510377No Hit
CATAGAGGTGAGCGGCTGTCTCCACCCAGTACAGGAGCACCCTCTATGTG1490.15284716309510377No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGGTCCGCGCAGAGCCTTCAGGTCA1480.15182134320855944No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTAGGATTACAAGTG1430.14669224377583784No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG1370.1405373244565719No Hit
CATAGACCATCCTCCCACCTTGACCTCCATCTTGACCTCCCAAAGTGCTG1360.1395115045700276No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGTATTACAGGTG1360.1395115045700276No Hit
CATAGACTACCACTACCCAGCACAGGAGCCACCCCATCCTACATGTGTCT1340.13745986479693897No Hit
CATAGAAGCAGCATTGATCATCCTCCATACGGTAGCATTTTAACACAGCA1320.1354082250238503No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCGGAGCCTTCAGGTCA1270.13027912559112872No Hit
CATAGACCATCCTCCCACCTTGACCTCCCCAGTGCTGGAATTACAGGAGT1270.13027912559112872No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA1250.12822748581804008No Hit
CATAGATCCCAGACTTTAGAAAATATACCATGTCACTCCTATGTAATGTC1220.1251500261584071No Hit
CATAGACCATCCTCCCACCTTGACCTTCCCGGTAGCTGGGACTACAGGCA1210.12412420627186278No Hit
CATAGAAGCAGCATTGATCATCCTCCATGTCGTCAGTGTGCTTCCCTGCT1210.12412420627186278No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGAGATTACACACG1200.12309838638531846No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGAGATTACAGAAG1180.12104674661222983No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGTCCGCGCAGAGCCTTCAGGTCAGA1170.12002092672568551No Hit
CATAGACCATCTGCGCATCCTGTCTGCTCTTTGACTTATGATGGAGTTCT1140.11694346706605253No Hit
CATAGAGAATACAATGAACTGTGATGGCATTGCTCTTTGACTTATGATGG1120.1148918272929639No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATGACAGGCA1110.11386600740641958No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGTTAGGATTACAGGCA1100.11284018751987526No Hit
CATAGACCATCTGCGCATCCTGTCTGCTACCACCACCCAGTACAGGAGCA1070.1097627278602423No Hit
CATAGACCATCCTCCCACCTTGACCTACGGTAGCAAAGGCTTGTGAGGGC1060.10873690797369798No Hit
CATAGATCCCAGGCTTTAGAAAATATACCAGATCCCAAGGCTTGTGAGGG1050.10771108808715366No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA1010.10360780854097638No Hit
CATAGATCCCAGGCTTTAGAAAATATACCATGTCGTCAGTGTAAGCTATA1000.10258198865443205No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA990.10155616876788773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)