FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40577
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG1768143.57394583138231No Hit
CATAGAGAATTTGCACACCACGGGACATGGATGAAGCTGGATCTATGAGA11922.9376247627966583No Hit
CATAGATTAGTTCTTATTCTTGGTATGGCACACACTGACGACATGGTTCT10742.6468196268822237No Hit
CATAGACCACACCCAAACACCACGTACACTGACGACATGGTTCTACACAT8222.0257781501836014No Hit
CATAGATGTCTCCCAGGCTAGAGTGAGCCCTTGTCCCTTTCTATGAGACC6801.6758262069645364No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAATCAGGGTTGGTTCTATGAGACC5151.2691919067452004No Hit
CATAGATTCTCCCTTGTGCTTTTGGTACATAGATTCTTCTCCCTTGTGCT4381.0794292333095101No Hit
CATAGATCATGGGACTTCAGCATGGTGGGTGCCTGATTGTTCTATGAGAC4221.0399980284397565No Hit
CATAGAGGGCCAGAAGTTTGTTTACCTACACTGACGACATGGTTCTACAC3570.8798087586563816No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGAGAGGCTGTTTGAGATCT3500.8625576065258643No Hit
CATAGAGAATTTGCACACCACGGGACCATGCTCACACCTTGATCTATGAG3200.788624097395076No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGCAACAGAACAACAACATCTATG3110.7664440446558395No Hit
CATAGACTTGATGCCCAGGAAACCTCCTGCCCCTGCAGGCTGAAAGTGCC3090.7615151440471203No Hit
CATAGAACAAGGCCAGAATTACCCTGGGTGAGAAATGATGATGGCTCTAT2660.6555437809596569No Hit
CATAGAGGGTGTTTTTCGGTGTTACAACCAATGCTCTTTCATCTATGAGA2650.6530793306552973No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGGAACAGAACAACAACATCTATG2490.6136481257855435No Hit
CATAGAGAAGAGAAGGCGACAATGGCCCCATCTCTACTAAAAATACAAAA2370.5840747221332282No Hit
CATAGAGGGTGTTTTTCGGTGTCACAACCAATGCTCTTTCATCTATGAGA2350.5791458215245089No Hit
CATAGAGGGTGTTTTTCGGTGTCACACACTGACGACATGGTTCTACACAT2180.5372501663503956No Hit
CATAGACCTTCCTGTTGGCCACACAGAATTAGCGGAGAGTGAGAACTCTA2150.5298568154373167No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCACTGACGACATGGTTCTACACAT1940.47810335904576484No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTCGCAGATTTGGACCTGCG1550.38198979717573994No Hit
CATAGAACAATGTAATAAGCATCGGAGGACACTGACGACATGGTTCTACA1530.37706089656702074No Hit
CATAGACAGGAGGAGGAAGAAGAGGAATTCTTGCCAGGGATCTATGAGAC1510.3721319959583015No Hit
CATAGACTTGATGCCCAGGAAACCTCTATACTTCAGTTGACTATAAGAAA1450.35734529413214383No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACCACGGGACATGGATGAAGC1390.3425585923059862No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGTGAGAAATGATGATGGCT1360.3351652413929073No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCCAGGCCTGAGATTGCTGCTAT1280.3154496389580304No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACATGGTGGGT1260.3105207383493112No Hit
CATAGATCATGGGACTTCAGCATGGCGGCGTTTGCAGATTTGGACCTGCG1240.30559183774059195No Hit
CATAGAATCAAAATAGTACCCTTGCCTCTACACTGACGACATGGGCAACA1210.2981984868275131No Hit
CATAGAATCAAAATAGTACCCTTGCCTCTATGAGACCAAGTCTCTGCTAC1150.28341178500135544No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGGCCTGCG1150.28341178500135544No Hit
CATAGAGAAGAGAAGGCGACAATGGCTCTATGCTGGGTGAGAAATGATGA1150.28341178500135544No Hit
CATAGAACAAGGCCAGAATTACCCTGGGAGAGGCTGTTTGAGATCTATGA1030.2538383813490401No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTGACGACATGGTGTTACAACC990.24398058013160162No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTCTGCAGATTTGGACCTGCG990.24398058013160162No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCGGATTTGGACCTGCG960.2365872292185228No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTGGGTGAGAAATGATGATGGC940.2316583286098036No Hit
CATAGACCCTTAGAGTTTCAGCCTCCTAGCTGGGACTTCAGTTGACTATA920.22672942800108434No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAGTGAGCCCTTGTCCCTTTCTATG910.22426497769672474No Hit
CATAGATTCTCCCTTGTGCTTTTGGTACACTGACGAGAGGTCTTGGCAGG900.22180052739236514No Hit
CATAGACAGCTCACAGACTGCATACTACACTGACGACATGGTGGGTGCCT890.2193360770880055No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACCACGGGACCATGCTCACAC880.2168716267836459No Hit
CATAGAGAAGAGAAGGCGACAATGGCTTGAACCCGGGAGGAGGTTGCAGC860.21194272617492668No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGCAACAGAACAACAACATC840.20701382556620745No Hit
CATAGATTCTCCCTTGTGCTTTTGGTACACTGACGACATGGTGGGTGCCT790.1946915740444094No Hit
CATAGACAGCTCACAGACTGCATACTTGGACTCCTTGGTCTAACACTCGG750.18483377282697094No Hit
CATAGACAGGAGGAGGAAGAAGAGGATAAAACAGGATTAGAGTTAAAAAG750.18483377282697094No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACATGGGCAAC720.17744042191389212No Hit
CATAGAGAAGAGAAGGCGACAATGGCCGCTAAGCCGAGAAGATCTCTATG710.1749759716095325No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCCGCG710.1749759716095325No Hit
CATAGAACAATGTAATAAGCATCGGAGGAGACCTGGGCAACAGAACAACA700.1725115213051729No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGCTTGCAGATTTGGACCTGCG700.1725115213051729No Hit
CATAGACTTGATGCCCAGGAAACCTCACACTGACGACATGGTGGGTGCCT680.16758262069645366No Hit
CATAGAACAAGGCCAGAATTACCCTGCACTGACGACATGGTGGGTGCCTG650.16018926978337483No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTCGGACCTGCG570.14047366734849792No Hit
CATAGACAGCTCACAGACTGCATACTGGGTGAGAAATGATGATGGCTCTA560.13800921704413832No Hit
CATAGATCATGGGACTTCAGCATGGTGGTGTTTGCAGATTTGGACCTGCG560.13800921704413832No Hit
CATAGAGCTATCAGTATACTCCCACCATGAGGAATTCTTGCCAGGGATCT510.12568696552234024No Hit
CATAGACTTGATGCCCAGGAAACCTCTAGAGAATTAGCGGAGAGTGAGAA510.12568696552234024No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGACTTGGACCTGCG500.12322251521798062No Hit
TAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCGAG490.12075806491362102No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATCTGGACCTGCG490.12075806491362102No Hit
CATAGACAGCTCACAGACTGCATACTACACTGGAGACAGCCGCTCACCTC490.12075806491362102No Hit
CATAGAGGGTGTTTTTCGGTGTCACAGATCTATGAGGCCTGAGATTGCTG490.12075806491362102No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGGTTTGGACCTGCG480.1182936146092614No Hit
CATAGAGAAGAGAAGGCGACAATGGCTGCTTTTCTCTTCTTGGCATCTAT470.1158291643049018No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTTATAGTCAAGGACTTTTAAG460.11336471400054217No Hit
CATAGATGTCTCCCAGGCTAGAGTGCGCCCTTGTCCCTTTCTATGAGACC450.11090026369618257No Hit
CATAGAGAATTTGCACACCACGGGACATGGATGAAGCTGGACCTATGAGA440.10843581339182295No Hit
CATAGATCTGTGGCTCCCGATAAATGTGCTCATACAGAATTAGATCTAGC430.10597136308746334No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGACACTGACGACATGGTGGGT430.10597136308746334No Hit
CATAGACAGCTGACCAGAAAATGTTTCAGTGTGGGCAACAGAACAACAAC420.10350691278310373No Hit
CATAGAACCCAAAGAGTGGGCAACAGAACAACAACATCTATGAGACCAAG410.10104246247874411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)