FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103500
Sequences flagged as poor quality0
Sequence length150
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA4025038.88888888888889No Hit
CATAGATTGCACATAAAGTCTGATCCCATCTCAAGTCTCACTCTTCTATG51444.970048309178744No Hit
CATAGAGCCTGGCATTTGAGGTAAGGCTTGTGAGGGCTGAAGTCTATGTG24292.3468599033816426No Hit
CATAGACTACCACTACCCAGCACAGGAGCACCCTCTATGTGTAGAACCAT19511.885024154589372No Hit
CATAGACTACCACTACCCAGCACAGCTACTTCTCTATGCACTTTGCCACA19021.8376811594202898No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGTGATTACAGGTG18661.8028985507246378No Hit
CATAGAAGCAGCATTGATCATCCTCCATTTTAACATCTTTAAGATCTTTA14961.4454106280193235No Hit
CATAGAAGCAGCATTGATCATCCTCCACCCCATCCTACATGTGTCTATGT14941.443478260869565No Hit
CATAGAGAATACAATGAACTGTGATGGCAGTGTAGGGATAGTTTGAGGGT11191.0811594202898551No Hit
CATAGACCATCCTCCCACCTTGACCTCTTAAGTAGCTGGGACTACAGGTG10731.0367149758454106No Hit
CATAGAGATGTCAAGAGCAATGCCCAGCCACCCCATCCTACATGTGTCTA10571.021256038647343No Hit
CATAGACTGTTCTTTTAAATTAACACAGTCAGAGAGGCACTTGGTCCAGA10531.017391304347826No Hit
CATAGACTACCACTACCCAGTACAGGAGCACCCTCTATGTGTAGAACCAT9000.8695652173913043No Hit
CATAGACTACCACCACCCAGTACAGGAGCACCCTCTATGTGTAGAACCAT8430.8144927536231883No Hit
CATAGACCATCCTCCCACCTTGACCTCCTGAGTAGCTGAGACCACAGGCA6660.6434782608695652No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAAGTGAAGGAGAGGCCAC6340.6125603864734299No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGTG5790.5594202898550724No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAGGTGTAAGGGAAGCCAC5780.5584541062801932No Hit
CATAGAGATGTCAAGAGCAATGCCCACCCCATCCTACATGTGTCTATGTG5670.5478260869565217No Hit
CATAGACCATCCTCCCACCTTGACCTCCCGAGTAGCTGGGACTACAGGTG5180.5004830917874397No Hit
CATAGAAGCAGCATTGATCATCCTCCAGCAGCATTTTAACACAGCATTGA4990.48212560386473424No Hit
CATAGACCATCCTCCCACCTTGACCTCATCCTCCCATCTCAAGTCTCACT4720.4560386473429952No Hit
CATAGAGATGTCAAGAGCAATGCCCATGCTGAAGTCCCATGATCTATGTG4240.4096618357487923No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAGAGTGCTGGAATTATAGGCG3700.35748792270531404No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGAGATTACACACG3690.3565217391304348No Hit
CATAGACTGTTCTTTTAAATTAACACAGTCAGAGACCTAGGATGGGTTTT3660.3536231884057971No Hit
CATAGACCATCCTCCCACCTTGACCTCTCGAGTAGCTGGGACTGCAGGCC3650.3526570048309179No Hit
CATAGATCCCAGGCTTTAGAAAATATACCACCCAGTACAGGAGCACCCTC3490.33719806763285026No Hit
CATAGATCCCAGGCTTTAGAAAATATACCATGTCGTCAGTGTAGGGATAG3420.3304347826086957No Hit
CATAGATCCCAGGCTTTAGAAAATATACCATCCCAGTCTTTTGCAAGTTT3400.32850241545893716No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTATTACTACAGGTG3400.32850241545893716No Hit
CATAGAAGGTGATAATCATGACAGCCACCCCATCCTACATGTGTCTATGT3120.3014492753623188No Hit
CATAGACCATCCTCCCACCTTGACCTCCATCTTGACCTCCCAAAGTGCTG2950.28502415458937197No Hit
CATAGACCATCTGCGCATCCTGTCTGACACTCTTGGTCTTTGTGGATCTA2920.2821256038647343No Hit
CATAGAAGCAGCATTGATCATCCTCCATGTCGTCAGTGTAGGGATAGTTT2850.2753623188405797No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGTCCGCGCAGAGCCTTCAGGTCAGA2760.26666666666666666No Hit
CATAGACCATCCTCCCACCTTGACCTTCCAAAGTGCTAGGATAATAGGTG2730.263768115942029No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGCG2710.2618357487922705No Hit
CATAGAAGGTGATAATCATGACAGCCAAGCCAGCCAGCCAGCCCAGACAG2540.24541062801932367No Hit
CATAGAGAATACAATGAACTGTGATGGCAGTGTCCCATCTCAAGTCTCAC2510.242512077294686No Hit
CATAGACTCAAGAAACTTACAATCATGGCATGTCTTGTCACTCCCTGGGA2510.242512077294686No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGCA2440.23574879227053142No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAACAGGTACTGGGATTACAGG2350.22705314009661837No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG2300.2222222222222222No Hit
CATAGACTGTTCTTTTAAATTAACACAGTCAGAGGCACTTGGTCCAGACT2060.19903381642512075No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGAG2030.19613526570048306No Hit
CATAGACCATCCTCCCACCTTGACCTCCAGAATAGCTGGGACTACAGGTG2010.19420289855072462No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA2000.1932367149758454No Hit
CATAGAGATGTCAAGAGCAATGCCCATGCCTAGCCATTTTTGGTTCTATG2000.1932367149758454No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAGGCAAAAAAGAAGCAGG1940.18743961352657004No Hit
CATAGACCATCCTCCCACCTTGACCTATCCTCCCATCTCAAGTCTCACTC1860.17971014492753623No Hit
CATAGATGACATAGACCTTATACCCTCATCAGTGTAGTCTTTTGCAAGTT1860.17971014492753623No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGGTCCGCGCAGAGCCTTCAGGTCA1810.1748792270531401No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA1710.16521739130434784No Hit
CATAGAGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA1700.16425120772946858No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGCCA1680.16231884057971013No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA1680.16231884057971013No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCGGAGCCTTCAGGTCA1650.15942028985507245No Hit
CATAGAGATGTCAAGAGCAATGCCCAGCCACATTATTTTCTATGTGTAGA1610.15555555555555556No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGGGCTGGGATGACACGCA1560.1507246376811594No Hit
CATAGACATGAGTGGCCGAACAGACCATGTCGTCAGTGTACTCCTATGTA1500.14492753623188406No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAGTAACTGGGACTACAGGTG1470.14202898550724638No Hit
CATAGAAGGTGATAATCATGACAGCCAAATGTGTGTGATAGTCAACTCTG1410.13623188405797101No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTAGGATTACAAGTG1380.13333333333333333No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGGGCCTTCAGGTCA1370.1323671497584541No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAAGGGAAGCAAACACGTC1330.1285024154589372No Hit
CATAGAGGTGAGCGGCTGTCTCCACACGTCCGCGCAGAGCCTTCAGGTCA1320.12753623188405797No Hit
CATAGATCCCAGGCTTTAGAAAATATACCACCACCCAGTACAGGAGCACC1250.12077294685990338No Hit
CATAGAGCCTGGCATTTGAGGTAAGGCACTTGGTCCAGACTTTCTATGTG1240.11980676328502415No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGAGATTACAGAAG1180.1140096618357488No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA1160.11207729468599033No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAAAGGAAGCAAGCATATC1090.10531400966183575No Hit
CATAGAGAATACAATGAACTGTGATGGCAGTCAAACCACAAAACACATTT1070.10338164251207729No Hit
CATAGAGATGTCAAGAGCAATGCCCGGCCACATTATTTTCTATGTGTAGA1050.10144927536231885No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGCGCCTTCAGGTCA1050.10144927536231885No Hit
CATAGACCATCTGCGCATCCTGTCTGAGAAGTGAGGAGCCCCTCCGCCCA1040.10048309178743962No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)