FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88162
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG1950022.118372995167988No Hit
CATAGAGCTTGGGTGCGAACATCAAGGAAGTTTCTGTGAGATCTATGAGA79308.994805018034981No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGTAATAACCAGGCCCCTCTATGAG50875.77005966289331No Hit
CATAGAGAAGACTCCCTCAACCCACCCACAGCCAGGAAAGAATCTATGAG44955.098568544270774No Hit
CATAGAAATGATGCTGAAGGGCCTGCCCTCTCTGCTGTTCCTTCTATGAG36434.132165785712665No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGTTCTGGGCCCCAGTGTGAA32803.7204237653410765No Hit
CATAGACGGATTATTCTCTCCATGGTCTACCAGTTATTAGTTGAATGAGC27163.080692361788526No Hit
CATAGACTCATTGCACTTTCCACAACTGTGTGCTTGAACCAGATTCTATG25312.870851387218983No Hit
CATAGACTCATTGCACTTTCCACAACTGTCCCAACTGTCTCTGAAAAATT17281.9600281300333477No Hit
CATAGACTCATTGCACTTTCCACAACTGTCATTGTCGCTGTCCCATCTAT16131.8295864431387674No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAGGAACCATGGGAGGTCTAT10871.2329575100383385No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGAAGTGATGGGCTAGATCTATGA9331.0582790771534223No Hit
CATAGACGTGACTAAAAGCATGTTGGTGTTTGCAGATTTGGACCTGCGAG8110.9198974614913455No Hit
CATAGATCAAGGAAAACACCAGCCCACGCTGTCCTTCTCTGAATCTATGA7420.8416324493545972No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAAGGAAGTTTCTGTGAGATC6260.710056486921803No Hit
CATAGATCATGGGACTTCAGCATGGCTCAGGTATGTCTTTATTAGCTCTA5980.6782967718518182No Hit
CATAGAGAAGACTCCCTCAACCCACCTTCCCCACACCCTCTTCTTCTATG5540.6283886481704135No Hit
CATAGAGTCTGAATGGTGGGAGAGTGAAAGAAATCTACAAAGGATTCTGG4850.5501236360336653No Hit
CATAGACCAGCAGACACTCCCATCATCAAGGAAGTTTCTGTGAGATCTAT4810.5455865338808102No Hit
CATAGACACAACAGAGAGAACGGGCTCACATAGACCCACGCTGTCCTTCT4620.5240352986547492No Hit
CATAGATCAAGGAAAACACCAGCCCAAGTCTCTGCTACCGTAACGCTATA3580.40607064268051996No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGCCCCAGTGTGAATACTTCT3340.37884802976339016No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGCTGAAGTGATGGGCTAGATCTA3340.37884802976339016No Hit
CATAGAAATGATGCTGAAGGGCCTGCACTGACGACATGGTTCTACACATA3260.36977382545768017No Hit
CATAGACCTCCGACAGTTTTTATGCACCCACAGCCAGGAAAGAATCTATG3010.3414169370023366No Hit
CATAGACCTCCGACAGTTTTTATGCACCTTCATTGTTGCTCTATGAGACC2730.30965722193235184No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTGCCTCTAAGTAAACTGAAT2700.3062543953177106No Hit
CATAGAGAAGACTCCCTCAACCCACGCTGTCCTTCTCTGAATCTATGAGA2680.3039858442412831No Hit
CATAGACCAGCAGACACTCCCATCATATAGAGATCCCACCATTTGCCATC2660.30171729316485557No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTCTGAAGGCTAATCTGAAAT2630.2983144665502144No Hit
CATAGAAAAAACAGTTGGCCAGGCATTGCTTCTTGTGGTAACTGGTCTAT2580.2926430888591457No Hit
CATAGAGTCCAACTATTCCAGCACCAAGGGGTAAGGCGTACTGGTCTATG2540.2881059867062907No Hit
CATAGACACAACAGAGAGAACGGGCTACACTGACGATCATTGTTCACTGC2450.277897506862367No Hit
CATAGATCACGTAGAAACTGGAGAGGCTAATCTGAAATCTTCGTGTTCTA2440.27676323132415326No Hit
CATAGAAATGATGCTGAAGTGATGGGCTAGATCTATGAGACCAAGTCTCT2430.27562895578593954No Hit
CATAGATGTTTTATCAAAGGCATGCTCATTGCTTCTTGTGGTAACTGGTC2410.27336040470951206No Hit
CATAGACACAACAGAGAGAACGGGCTAGATCTGCTGAAGTGATGGGCTAG2340.26542047594201584No Hit
CATAGAAATGATGCTGAAGGGCCTGCCATGGTTCTACACATAGAAATGAT2310.2620176493273746No Hit
CATAGAAATGATGCTGAAGGGCCTGCTACACATAGAAATGATGCTGAAGT2250.25521199609809214No Hit
CATAGAAATGATGCTGAAGGGCCTGGTTCTACACATAGAAATGATGCTGA2150.2438692407159547No Hit
CATAGAGCTGTACTGCATCTTTTCTCAAACTGCTGGGGTTGCTCTATGAG2110.23933213856309976No Hit
CATAGAGTCTGAATGGTGGGAGAGTGACGACATGGTTCTACACATAGAGT2100.238197863024886No Hit
CATAGAAAATTTGTGATGGTAAATCATGGGAGGTCTATGAGACCAAGTCT2090.23706358748667228No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTCTTCCTTTTCTGCCTTCATCTA2040.23139220979560357No Hit
CATAGACCCAGATGCTGTCCCACTAACTTGGGCCCCAGTGTGAATACTTC1940.22004945441346613No Hit
CATAGACTCATTGCACTTTCCACAACTGTGGCCCTGTCCCAGGGCTGTGC1870.21210952564596994No Hit
CATAGAAATGATGCTGAAGGGCCTGCCTGACGACATGGTTCTACACATAG1860.21097525010775617No Hit
CATAGACCAGCAGACACTCCCATCATGATGTAGTACTGAAGCCCTTCTCT1860.21097525010775617No Hit
CATAGACTCATTGCACTTTCCACAACTGTTTCCACAACTGACGACATGGT1820.20643814795490123No Hit
CATAGAAATGATGCTGAAGGGCCTGCGATGCTGAAGTGATGGGCTAGATC1790.20303532134025998No Hit
CATAGAAAATTTGTGATGGTAAATCATGGGACATGCCAGGAATCAATGTT1770.2007667702638325No Hit
CATAGACCGCTTCCCAATTTTTGTAACACACTGTCATTGTCGCTGTCCCA1640.18602118826705383No Hit
CATAGAGTCCAACTATTCCAGCACCACTGGATCAGCACATCCACACACTC1560.1769469839613439No Hit
CATAGAAAATTTGTGATGGTAAATCATGGGATCATCAGGAACCATGGGAG1470.1667385041174202No Hit
CATAGAAATGATGCTGAAGGGCCTGCCACTGACGACATGGTTCTACACAT1450.1644699530409927No Hit
CATAGATCAAGGAAAACACCAGCCCACCCACAGCCAGGAAAGAATCTATG1400.158798575349924No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGGGGTAAGGCGTACTGGTCTATGA1350.1531271976588553No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTCTGAAAAATTTCTATGAGA1250.14178444227671785No Hit
CATAGAAATGATGCTGAAGGGCCTGCACACTGACGACATGGTTCTACACA1240.1406501667385041No Hit
CATAGACCAACATTTCTTCTCCCAGGGTCTACCAGTTATTAGTTGAATGA1220.13838161566207663No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTCATCAAGGAAGTTTCTGTG1220.13838161566207663No Hit
CATAGACCTCCGACAGTTTTTATGCACTGTCCCAACTGTCTCTGAAAAAT1210.1372473401238629No Hit
CATAGATGTGTAGCCTGGGTCTGACATGCCAGGAATCAATGTTCTATGAG1200.13611306458564915No Hit
CATAGATCAAGGAAAACACCAGCCCAGGTCTCCCTTGCAGAAGTAGGAAG1180.13384451350922166No Hit
CATAGAGTGCCTGTTTCCTCATTCGTCAGTGTGGACATGCCAGGAATCAA1170.13271023797100792No Hit
CATAGACGTGACTAAAAGCATGTTGGTGACGACATGTTCTACACATAGAG1140.1293074113563667No Hit
CATAGACCAGCAGACACTCCCATCATTTATTTTAAAGGACTGGAAGCCTC1140.1293074113563667No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGTAGATTTGGACCTGCG1120.12703886027993921No Hit
CATAGAGTCCAACTATTCCAGCACCACACTGACGACATGGTTCTACACAT1100.12477030920351172No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGACGACATGGCTCAGGT1080.12250175812708423No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGGCCTGCG1050.119098931512443No Hit
CATAGATCCACGTGAGAATAAAGCATACACTGACGACATGGTTCTACACA980.1111590027449468No Hit
CATAGAGTAGTTAAAACCCACAATGACAGTGACGACATGGTTCTACACAT960.10889045166851932No Hit
CATAGAAAAAACAGTTGGCCAGGCATATTAACTGGGTGTGGTGGCATCTA920.10435334951566434No Hit
CATAGACTCATTGCACTTTCCACAACTGCCCTCTCTGCTGTTCCTTCTAT910.10321907397745061No Hit
CATAGAAATGATGCTGAAGGGCCTGCATAGAAATGATGCTGAAGTGATGG910.10321907397745061No Hit
CATAGACAGTGCCTTTGTGCGTGTTACACTGACGACATGGTTCTACACAT900.10208479843923687No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTCTGAAAAATTTCGTCCCAA900.10208479843923687No Hit
CATAGATGTTTTATCAAAGGCATGCTCATTTATTTTAAAGGACTGGAAGC890.1009505229010231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)