FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005806981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005806981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164973
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG3792922.991034896619446No Hit
CATAGAGAGATTTTGGGCTGAGACGATAATGCCGCATATCTACAACTATC85335.172361537948634No Hit
CATAGAGCCAAGGAAAGTTGAATGGAGACAGCCGCTCACCTCTATGAGAC56263.4102550114261123No Hit
CATAGATCATGGGACTTCAGCATGGAGACAGCCGCTCACCTCTATGAGAC42782.5931516066265385No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTTTCTAGATATACACTCA41692.5270801888793923No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAACCTGTCTCTACT38972.3622047244094486No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTTTCTAGATATACAACAA30121.8257533050862869No Hit
CATAGAACTTCACAAGGGCTTTAGGGGCTACACATGGCTATGCAATCTAT27131.6445115261285181No Hit
CATAGACCTGGGCTTAGGGGCTACACATGGCTATGCAATCTATGAGACCA27051.6396622477617548No Hit
CATAGACCTGGGCTTAGGGGCTATACATGGCTATGCAATCTATGAGACCA26161.5857140259315161No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTTTCTAGATATACAATCA26001.57601546919799No Hit
CATAGATCATGGGACTTCAGCATGGCTACACATGGCTATGCAATCTATGA19121.158977529656368No Hit
CATAGACACCATGCCTGGCTAATTTTTTGCGTTTTTAGTAGAGATGGGGT18671.1317003388433258No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCCCATCTCTA18311.1098785861928921No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGGGCAAGCAATGACAG16280.9868281476362799No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCCCGTCTCTA14890.902571936013772No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGGGGAAAGGATTCTAT13430.8140726058203464No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCCTGTCTCTA12980.7867954150073042No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTTCCTTCTGCTGGCTATTCTATG11940.7237547962393847No Hit
CATAGATCCTCTCATTGTCCAGTTTGGAGACAGCCGCTCACCTCTATGAG10230.6201014711498245No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGATAGGGTT10080.6110090742121438No Hit
CATAGATCCTCTCATTGTCCAGTTTGGTACGGTTGCACAGATCATTTAAG9160.5552423729943687No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTGGAGACAGCCGC8150.49402023361398534No Hit
CATAGACCTTTGGGTTCATATCATCTGGAGACAGCCGCTCACCTCTATGA7870.4770477593303147No Hit
CATAGACCTGGGCTTAGGGGCTATACGTGAATGTGGATCAGAACTTCTAT7530.4564383262715717No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTATAGAGCAGAATTACCTCTGGA7030.42613033647930265No Hit
CATAGACAATTAAATTTTCTTAAGGGGTTGCACAGATCATTTAAGCTCAA6620.4012777848496421No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTATCCCCAGAAATGTTAGCATGT4610.2794396658847205No Hit
CATAGATCATGGGACTTCAGCATGGCACACTGACTGATACAGGAAGGCCT4580.27762118649718437No Hit
CATAGACACCATGCCTGGCTAATTTTTAAATATTTTGTAGAGACCGGGTT4280.259436392621823No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTTGTAGAGACAGAGTT4210.2551932740509053No Hit
CATAGACAGGGAGGCGTATAAGTGATGGAGACAGCCGCTCACCTCTATGA4180.25337479466336915No Hit
CATAGACACCATGCCTGGCTAATTTTTTGTATTTTTAGTGGAGACAGCCG4000.2424639183381523No Hit
CATAGATCCCATCCAATAATAATGAGCAGAATTACCTCTGGATGGTCTAT3600.21821752650433707No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGCGTATTCTAAATATACAATCA3500.21215592854588328No Hit
CATAGATGCCCTTTTTGTGTACTTCCACATGAGACATGGTTCTACACATA3480.2109436089541925No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGCGAAACCCCATCTCTA3150.19094033569129493No Hit
CATAGATCATGGGACTTCAGCATGGCTAATTTTTGTATTTTTAGTGGAGA3140.19033417589544957No Hit
CATAGATGAAAGCCTGTTGAATTTATGACCTGATTGGCCATTTGAACTCT3100.18790953671206803No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCTCGTCTCTA2980.18063561916192347No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCCGCG2960.1794232995702327No Hit
CATAGATCATGGGACTTCAGCATGGCTAATTTTTTGCGTTTTTAGTAGAG2870.17396786140762427No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAATCCCATCTCTA2860.17336170161177888No Hit
CATAGAAACCCCAAAGGCTATGTTCAAAATGGTCAAACAGGAGGTCTATG2840.17214938202008814No Hit
CATAGATGAAAGCCTGTTGAATTTATGAGACCAAGTCTCACCTGATTGGC2830.17154322222424276No Hit
CATAGATCATGGGACTTCAGCATGGCCAATATGGTGAAACCCTGTCTCTA2800.1697247428367066No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGTGGTGTGGTG2740.16608778406163432No Hit
CATAGACACCATGCCTGGCTAATTTTGTATTTTTAGTAGAGACAGGGTTT2720.16487546446994356No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTACACTGACTGATACAGGAAGGC2710.1642693046740982No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTCTCTAAATATACACTCA2600.157601546919799No Hit
CATAGAGCTGGCTTCCTAACTTCCAAGCTGCCTTCCTAACCATGGGCATA2600.157601546919799No Hit
CATAGAAACCCCAAAGGCTATGTTCAGTGTACCATTTTCCCCCAAGTCAT2540.15396458814472672No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGCAAAACCCCATCTCTA2520.15275226855303595No Hit
CATAGATCCCATCCAATAATAATGAACAAGCAATGGGGAAAGGATTCTAT2490.1509337891654998No Hit
CATAGACACCATGCCTGGCTAATTTTAAAATTAGTACCTATAATCTCAGC2480.15032762936965444No Hit
CATAGATCATGGGACTTCAGCATGGCTATGCAATCTATGAGACCAAGACA2440.1479029901862729No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCCCGTAACAAGCAATGGGGAAA2400.1454783510028914No Hit
CATAGATCATGGGACTTCAGCATGGCTAATTTTTGTATTTTTAGTAGAGA2390.144872191207046No Hit
CATAGAGCTGGCTTCCTAACTTCCAAACAATGGGCAAGCAATGACAGTCT2360.14305371181950988No Hit
CATAGATCATGGGACTTCAGCATGGCAAAACCTTGACTCCACTAAAAATA2280.1382044334527468No Hit
CATAGATCATGGGACTTCAGCATGGCGTCAGTGTTGGAGACAGCCGCTCA2210.13396131488182914No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATAGTGAAACCCTGTCTCTA2190.1327489952901384No Hit
CATAGACACCATGCCTGGCTAATTTTTTGTATTAATCCCAGCTACTCAGG2180.132142835494293No Hit
CATAGACACCATGCCTGGCTAATTTTTTGTATTTTTAGTAGAGATGGGGT2160.13093051590260224No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTCGCAGATTTGGACCTGCG2150.13032435610675686No Hit
CATAGACCTTTGGGTTCATATCATCTGGACACTGACTGATACAGGAAGGC2100.12729355712752996No Hit
CATAGATGAAAGCCTGTTGAATTTATGAGACCAAGTCTACCTGATTGGCC2090.12668739733168458No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCTTGTCTCTA2060.12486891794414844No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGCGAAACCCCGTCTCTA2000.12123195916907616No Hit
CATAGAGGATCCCATCTGAGTTGGTCTCATAGATGGAGACAGCCGCTCAC1940.11759500039400386No Hit
CATAGATCATGGGACTTCAGCATGGCCAATATGGTGAAACCCCATCTCTA1900.11517036121062234No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATAGTGAAACCCCATCTCTA1870.1133518818230862No Hit
CATAGATCATGGGACTTCAGCATGGCGGCGTTTGCAGATTTGGACCTGCG1860.11274572202724083No Hit
CATAGATCATGGGACTTCAGCATGGCACTGGTACCAAAACAGAGATATAG1860.11274572202724083No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACTCCATCTCTA1840.11153340243555006No Hit
CATAGATCATGGGACTTCAGCATGGCAAAACCCCGTCTCTGCTAAAAACA1820.1103210828438593No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTACCATGTTTTCATCCTG1780.10789644366047776No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGACAATGGGCAAGCAA1780.10789644366047776No Hit
CATAGATCATGGGACTTCAGCATGGCTAATTTTTTGTATTTTTAGTGGAG1770.1072902838646324No Hit
CATAGAGCTGGCTTCCTAACTTCCAAGTCTCTGCACCTGATTGGCCATTT1750.10607796427294164No Hit
CATAGATCATGGGACTTCAGCATGGCAAAACCCCATCTCTACTAAAAATA1750.10607796427294164No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTCTGCAGATTTGGACCTGCG1680.10183484570202396No Hit
CATAGATCATGGGACTTCAGCATGGCTATGCAATCTATGAGACCAAGTCT1650.10001636631448783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)