FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301574
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGAATTTGCACACCACGGGACATGGATGAAGCTGGATCTATGAGA3122910.35533567217333No Hit
CATAGATGTCTCCCAGGCTAGAGTGAGCCCTTGTCCCTTTCTATGAGACC205806.8241957197901675No Hit
CATAGATCATGGGACTTCAGCATGGTGGGTGCCTGATTGTTCTATGAGAC152915.070397315418438No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAATCAGGGTTGGTTCTATGAGACC145164.813412296816039No Hit
CATAGAGAATTTGCACACCACGGGACCATGCTCACACCTTGATCTATGAG89582.970415221471347No Hit
CATAGAACAAGGCCAGAATTACCCTGGGAGAGGCTGTTTGAGATCTATGA88912.948198452121204No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGTGAGAAATGATGATGGCT84322.7959970023941056No Hit
CATAGACCTTCCTGTTGGCCACACAGAATTAGCGGAGAGTGAGAACTCTA50311.66824726269506No Hit
CATAGAGGGTGTTTTTCGGTGTTACAACCAATGCTCTTTCATCTATGAGA43951.4573537506548975No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGAGAGGCTGTTTGAGATCT41011.3598652403721807No Hit
CATAGAACAAGGCCAGAATTACCCTGGGTGAGAAATGATGATGGCTCTAT40621.3469330910489632No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACATGGGCAAC35321.171188497682161No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGCAACAGAACAACAACATCTATG35251.1688673426754295No Hit
CATAGACCCTTAGAGTTTCAGCCTCCACACTGACGACATGGTTCTACACA34831.1549404126350415No Hit
CATAGATTAGTTCTTATTCTTGGTATGGCACACACTGGAGACAGCCGCTC32221.0683944902412013No Hit
CATAGACAGCTCACAGACTGCATACTACACTGACGACATGGTGGGTGCCT30541.012686770079649No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGCAACAGAACAACAACATC30361.0067180857766251No Hit
CATAGACAGGAGGAGGAAGAAGAGGAATTCTTGCCAGGGATCTATGAGAC29510.978532632123459No Hit
CATAGACAGCTGACCAGAAAATGTTTCAGTGTACTTCAGTTGACTATAAG28990.9612897663591689No Hit
CATAGAGGGTGTTTTTCGGTGTCACAACCAATGCTCTTTCATCTATGAGA28610.9486892106083414No Hit
CATAGACCACACCCAAACACCACGTACACTGACGACATGGTTCTACACAT27850.9234880991066868No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACCACGGGACATGGATGAAGC26380.8747438439653286No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAGTGAGCCCTTGTCCCTTTCTATG25590.8485479517465033No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTCTATGAGACCAAGTCTCTGC24360.8077619423425096No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGAGACAGCCGCTCACCTCTA24230.8034512259014371No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGGAACAGAACAACAACATCTATG23990.7954929801640725No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCCAGGCCTGAGATTGCTGCTAT22610.7497330671742259No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGACACTGGAGACAGCCGCTCA22450.7444275700159828No Hit
CATAGAGAAGAGAAGGCGACAATGGCTGCTTTTCTCTTCTTGGCATCTAT22220.7368009178510083No Hit
CATAGACAGGAGGAGGAAGAAGAGGAGAATTAGCGGAGAGTGAGAACTCT20530.6807616041170658No Hit
CATAGACAGCTGACCAGAAAATGTTTCACTATGAGACCAAGTCGACTCTT19540.6479338404504367No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACTCTTCACTA18490.6131165153494665No Hit
CATAGAACAAGGCCAGAATTACCCTGTGCTCATACAGAATTAGATCTAGC17730.5879154038478118No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGACACTGACGACATGGTGGGT17460.5789623773932766No Hit
CATAGATTCTCCCTTGTGCTTTTGGTACACTGACGACATGGTGGGTGCCT17310.5739884738074237No Hit
CATAGATCTGTGGCTCCCGATAAATGTGAGGAATTCTTGCCAGGGATCTA16780.5564140144707435No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTGGGAGAGGCTGTTTGAGATC16220.5378447744168927No Hit
CATAGAATCAAAATAGTACCCTTGCCTCTACACTGATCTGAGCAAGGATA13930.4619098463395386No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGATCTGAGCAAGGAT13780.4569359427536857No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAGAGTGAGCCCTTGTCCCTTTCTA12900.42775570838334864No Hit
CATAGACAGCTGACCAGAAAATGTTTCAGTGTACCAGCAGAATCAGGGTT12550.4161499333496919No Hit
CATAGAGCTATCAGTATACTCCCACCATGAAATACTGGGGACCATGCTCA12400.41117602976383905No Hit
CATAGAATGCAGGAGAATATACACAGGTTACACTGATCTGAGCAAGGATA11980.39724909972345096No Hit
CATAGATGTCTCCCAGGCTAGAGTGCGCCCTTGTCCCTTTCTATGAGACC11690.3876328861241354No Hit
CATAGAACAATGTAATAAGCATCGGAGGACCATGCTCACACCTTGATCTA11640.3859749182621844No Hit
CATAGACTTGATGCCCAGGAAACCTCAGACATGTTTCCTAGTTTCAGTTC10940.3627633681948709No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACCACGGGACCATGCTCACAC10860.36011061961574936No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAACCCAAAGAGTGAGCCCTTGTCC10440.3461836895753613No Hit
CATAGAATGCAGGAGAATATACACAGGTTGACAGTGTGCTGCTTTTCTCT10420.3455205024305809No Hit
CATAGAGGGTGTTTTTCGGTGTCACAGATCTAGAGGCCTGAGATTGCTGC10230.3392202245551672No Hit
CATAGAATGCAGGAGAATATACACAGGTTACACTGACGACATGACTCTTC10180.33756225669321627No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCACTGACGACATGGTTCTACACAT10180.33756225669321627No Hit
CATAGATCATGGGACTTCAGCATGGCGGGTGGTCTTACTCTTCCCGTCTA9850.3266196688043399No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCTTGGGTGACAGAGTGAGTCTATG9810.3252932945147791No Hit
CATAGAGGGCCAGAAGTTTGTTTACCTGTCGTCAGTGAGGAATTCTTGCC9760.3236353266528282No Hit
CATAGACCCTTAGAGTTTCAGCCTCAGACATGTTTCCTAGTTTCAGTTCT9750.32330373308043797No Hit
CATAGAGAAGAGAAGGCGACAATGGGACATGGATGAAGCTGGATCTATGA9670.3206509845013164No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGGGACATGGATGAAG9480.31435070662590275No Hit
CATAGAGAATTTGCACACCACGGGACATGGATGAAGCTGGACCTATGAGA9030.2994289958683441No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGTGCTCATACAGAATTAGATC8780.29113915655858924No Hit
CATAGAACCCAAAGAGTGAGCCCTTGTCCCTTTCTATGAGACCAAGTCTC8540.28318091082122465No Hit
CATAGATTCTCCCTTGTGCTTTTGGTACACTGACGACATGGTTCTACACA8520.28251772367644423No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACATGGTGGGT7680.25466386359566806No Hit
CATAGATCTGTGGCTCCCGATAAATGTACACTGACGACTCTTCACTATGT7610.2523427085889367No Hit
CATAGAGGGTGTTTTTCGGTGTCACACAGAATTAGCGGAGAGTGAGAACT6820.22614681637011147No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCCCAAAGAGTGAGCCCTTGTCCCT6500.21553582205362534No Hit
CATAGATTCTCCCTTGTGCTTTTGGTTACACATAGAGAATTAGCGGAGAG6440.21354626061928414No Hit
CATAGACAGCTGACCAGAAAATGTTTCAGTGTGTGCTCATACAGAATTAG6380.211556699184943No Hit
CATAGACAGGAGGAGGAAGAAGAGGAGGGACATGGATGAAGCTGGATCTA6330.20989873132299203No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCAGCAGAATCAGGGTTGGTTCT5950.19729817557216472No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCCAGGCTAGAGTGAGCCCTTGT5880.19497702056543337No Hit
CATAGAGGGCCAGAAGTTTGTTTACCTACACTGACGACATGGTGGGTGCC5820.1929874591310922No Hit
CATAGAGGGCCAGAAGTTTGTTTACCTACACTGACACATGGTGGGTGCCT5760.19099789769675105No Hit
CATAGATGTCTCCCAGGCTAGAGTGAGCCCTTGTCCCTTTCCATGAGACC5680.1883451491176295No Hit
CATAGAGCAAAAGAGAAACATTAAGCCATAGTACACTGACGACATGGTGG5650.1873503684004589No Hit
CATAGAATCAAAATAGTACCCTTGCCTCTGACACTGACGACATGGTGGGT5600.18569240053850797No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTCGGCAGTGAGGTACAGAAAT4840.1604912890368533No Hit
CATAGAGGGCCAGAAGTTTGTTTACCTAGAGGCTGCTTTTCTCTTCTTGG4680.15518579187861023No Hit
CATAGACAGCTCACAGACTGCATACTATGTGTAGATGGGCAACAGAACAA4360.14457479756212405No Hit
CATAGAGCTGCTTTTCTCTTCTTGGCATCTATGAGACCAAGTCTCTGCTA4340.14391161041734365No Hit
CATAGAACCCAAAGAGTGAGCAGCAGGAACCCAAAGAGTGAGCCCTTGTC4250.14092726826583193No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCCGACATGGTGGGTGCCTGATT4220.13993248754866136No Hit
CATAGAACCCAAAGAGTGAGCAGCAGACCCAAAGAGTGAGCCCTTGTCCC4140.13727973896953982No Hit
CATAGATCATGGGACTTCAGCATGGTGGGTGCCTGATTGTTTTATGAGAC4090.13562177110758886No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAAGAGTGAGCCCTTGTCCCTTTCT4030.13363220967324768No Hit
CATAGACTTGATGCCCAGGAAACCTCACTTCAGTTGACTATAAGAAACAC4010.1329690225284673No Hit
CATAGAACAATGTAATAAGCATCGGAGGACACTGACGACATGGTTCTACA3750.12434758964632231No Hit
CATAGACCACACCCAAACACCACGTACTGACGACATGGTTCTACACATAG3680.12202643463959095No Hit
CATAGAACCCAAAGAGTGAGCAGCAGTAGAACCCAAAGAGTGAGCCCTTG3630.12036846677763997No Hit
CATAGATCATGGGACTTCAGCATGGAGACAGCCGCTCACCTCTATGAGAC3510.11638934390895767No Hit
CATAGATCATGGGACTTCAGCATGGCACACTGACGACATGGTTCTACACA3500.11605775033656747No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCATAGAACCCAAAGAGTGAGCCCT3490.11572615676417729No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAATCAGGGTTGGTTCCATGAGACC3400.11274181461266555No Hit
CATAGACCCTTAGAGTTTCAGCCTCCAGCAGAATCAGGGTTGGTTCTATG3400.11274181461266555No Hit
CATAGAGGGTGTTTTTCGGTGTCACACACTGACGACATGGTTCTACACAT3390.11241022104027534No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCCAGGCAGTCTCAAACGTTGTG3390.11241022104027534No Hit
CATAGATCTGTGGCTCCCGATAAATGTACACTGACGACATGGTGGGTGCC3370.11174703389549498No Hit
CATAGATGTCTCCCAGGCTAGAGTGCTCATACAGAATTAGATCTAGCTCT3340.11075225317832438No Hit
CATAGAACCCAAAGAGTGAGCAGCAGTTCTACACATAGAACCCAAAGAGT3300.10942587888876362No Hit
CATAGATCATGGGACTTCAGCATGGCGGGTGCCTGATTGTTCTATGAGAC3260.10809950459920285No Hit
CATAGAACCCAAAGAGTGAGCAGCAGGTTCTACACATAGAACCCAAAGAG3060.101467633151399No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)