FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142993
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG4746133.19113523039589No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCGTGGAGGAAAGGAGGTCTATGAG83705.853433384851007No Hit
CATAGAGCTTCTGATCACTTTCGCCCCACCCTTATGATCGTATCTATGAG64134.484834921989188No Hit
CATAGAACTTGTAATCATTCCCTACTTCCCACACTCATGCAAAGCTGGTC49803.482687963746477No Hit
CATAGAACCAGAGTAATGAGGGATGCTCAGGTCTCCAATGGTGTCTATGA49213.4414272027302037No Hit
CATAGAACCAGAGTAATGAGGGATGCTCTCTCCAGAACCCACCATCTATG38952.7239095620065314No Hit
CATAGATGTGGCTATGCAGACTGAGCAGAGTTGGGTGTGTCTATGAGACC34142.3875294594840306No Hit
CATAGATGTGGCTATGCAGACTGAGTAGAGTTGGGTGTGTCTATGAGACC17941.2546068688677068No Hit
CATAGACTCAAGCAATCCTCCCACCTCAGCCTCCCAAATAGCCAGGACTA16671.1657913324428468No Hit
CATAGATCATGCAAAGAACACAGGCCACACTCATGCAAAGCTGGTCTATG15721.0993545138573217No Hit
CATAGAACTTTGTGCCCCCAGTGTCTTAGGTAGAGGAGTTTCAAACATCT15411.0776751309504662No Hit
CATAGACTCAAGCAATCCTCCCACCTCAGCCTCCCAAGTAGCCAGGACTA14781.0336170302042758No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATGAGACCAAGTCTCTG14220.9944542739854398No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCAGATTTGGACCTGCGAGCGGGTTC13400.9371088095221445No Hit
CATAGATCATGCAAAGAACACAGGCAGATCATGAGGTCAGGTCTATGAGA11910.8329079045827418No Hit
CATAGATGTGGCTATGCAGACGGAGTGAGGGCAGTAAGTCTATGAGACCA10550.7377983537655689No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCGTGGAGGAAAGCAATCACCGCCA9170.6412901330834376No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCGTGGAGGGAAGGAAGGGCAAACT8960.6266040995013742No Hit
CATAGACCCTTCTCCATGCCACATCAGGGATTATCAGTAGTGCTCACATC8820.6168134104466652No Hit
CATAGATGTGCCTCTCCAACATTCATTTCCCCTCTCTCTGAATAACTCTA8150.5699579699705579No Hit
CATAGAACTTTGTGCCCCCAGTGTCTTCTTATAGCCAGTGTCTCCCCTCT7580.5300958788192428No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTAGTGCAGCTATATTGGAGGAA7560.5286972089542844No Hit
CATAGACCCTTCTCCATGCCACACTCATGCAAAGCTGGTCTATGAGACCA7240.5063184911149498No Hit
CATAGATCTAGTACTGTAATTGTAGTGTGCAACACTGACGACATGGTTCT6820.47694642395082276No Hit
CATAGATGTGGCTATGCAGACTGAGTGAGGGCAGTAAGTCTATGAGACCA6160.4307903184071948No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTTCTTATAGCCAGTGTCTCC6060.42379696908240266No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCATGTCTTCTGTGTTTGGTCTATG6050.4230976341499234No Hit
CATAGACCATTTGCCGCGTTCTGAAGATCATTTCCCCTCTCTCTGAATAA5130.3587588203618359No Hit
CATAGAAGATCCCATAGCAGCTTTGCCGTGGAGGAAAGGAGGTCTATGAG5120.35805948542935667No Hit
CATAGAACACAGATCTGCTTGGAGGGAAGGAAGGGCAAACTCTATGAGAC4490.3140013846831663No Hit
CATAGATCATGGGACTTCAGCATGGCTAGTCCGTCACCCCTAATCTATGA4400.3077073702908534No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCAGCATGTTGAGATGAATAAT4260.29791668123614445No Hit
CATAGAGGAAGTGCCTACCTTAAATTGATGGAGACAGCCGCTCACCTCTA4220.2951193415062276No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATGAGACCAAGTTCTTA4220.2951193415062276No Hit
CATAGATCAACAATGGATGAGCAAAAGGGATTATCAGTAGTGCTCACATC4220.2951193415062276No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGGCTAGTCCGTCACCCCTAATCT3760.2629499346121838No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCTCTGAGCATGTTGAGATGAA3690.25805459008482934No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTTCTTATAGCCAGTGTCT3670.25665592021987094No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGGAGGGAAGGAAGGGCAAACTCT3350.23427720238053612No Hit
CATAGAAGATGGTCTGCTACACTGGTGTTCCACACTCATGCAAAGCTGGT3260.2279831879882232No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGGGAGGGAAGGAAGGGCAAACTC3160.22098983866343105No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATGAGACCAAGTCTCTT3090.2160944941360766No Hit
CATAGAAGATCCCATAGCAGCTTTGCCATGTCTTCTGTGTTTGGTCTATG3030.21189848454120133No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCTTCTTATAGCCAGTGTCTCCC3000.20980047974376367No Hit
CATAGAGCTTCTGATCACTTTCGCCCTCTCTCCAGAACCCACCATCTATG3000.20980047974376367No Hit
CATAGACACCTGGATCCTGTGTTGTCTCATTTCCCCTCTCTCTGAATAAC2980.20840180987880524No Hit
CATAGAAGGAGGGGAAACTGAGGCCACACTCATGCAAAGCTGGTCTATGA2960.20700314001384684No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATTCTTATAGCCAGTGT2870.20070912562153392No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTCGCAGATTTGGACCTGCG2840.19861112082409627No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATGAGACCAAGTCTTCT2750.19231710643178337No Hit
CATAGATCATGGGACTTCAGCATGGCATGTTGAGATGAATAATTGAGACT2650.18532375710699125No Hit
CATAGAAGGAGGGGAAACTGAGACCAAGTCTCTGCTACCGTAGGTGTCTT2470.17273572832236542No Hit
CATAGAAGAATTGCTTGAATCCGGGAGGGAAGGAAGGGCAAACTCTATGA2470.17273572832236542No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCTTCTTATAGCCAGTGTCTCCCCT2300.16084703447021884No Hit
CATAGACCATTTGCCGCGTTCTGAAGGCAGATCATGAGGTCAGGTCTATG2260.15804969474030198No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCGGATTTGGACCTGCG2250.15735035980782275No Hit
CATAGATCATGGGACTTCAGCATGGCGGCGTTTGCAGATTTGGACCTGCG2110.1475596707531138No Hit
CATAGAGTCTCGCTAATAGTTTTACCTCAAAGAGTCTCGCTAATAGTTTT2100.1468603358206346No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGGCCCCACCCTTATGATCGTATC2050.1433636611582385No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCTCTCCAGAACCCACCATCTATGA2040.1426643262257593No Hit
CATAGACCCTTCTCCATGCCACATCATGCAAAGCTGGTCTATGAGACCAA2040.1426643262257593No Hit
CATAGAACACAGATCTGCTTGGAGGTGGGAGCATCTGTGGGAAGGTTCAG2040.1426643262257593No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCA2020.14126565636080088No Hit
CATAGAACACAGATCTGCTTGGAGGTCTATTGCCGTGGAGGAAAGGAGGT1990.13916765156336325No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGCTTGCAGATTTGGACCTGCG1950.1363703118334464No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTCTGCAGATTTGGACCTGCG1950.1363703118334464No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATCTGGACCTGCG1940.1356709769009672No Hit
CATAGAACTTGTAATCATTCCCTACTTCCCCTCTCTCCAGAACCCACCAT1940.1356709769009672No Hit
CATAGATGTGGCTATGCAGACTGAGTTTTTCCAAATATAAGATCCCATCA1900.13287363717105033No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCTTCTTATAGCCAGTGTCTCCCC1730.12098494331890372No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCCGCG1730.12098494331890372No Hit
CATAGATCATGGGACTTCAGCATGGCATGGGACTTCAGCATGTTGAGATG1690.11818760358898686No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGACTTGGACCTGCG1650.11539026385907003No Hit
CATAGATGTGCCTCTCCAACATTCACCAAGTCACCCAGCCTCTATGAGAC1640.11469092892659082No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGGCCTGCG1590.11119425426419476No Hit
CATAGAAGAATTGCTTGAATCCGGGAGGCAGAGGTTGCAGTGAGCTGAGA1580.11049491933171553No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAATGGCATTAAATGACTTGCCCAA1540.1076975796017987No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTCGGACCTGCG1540.1076975796017987No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCTCTGCTACCGTATTCACCAA1480.10350157000692342No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCTCTGCTACCGTGGAGACAGC1470.10280223507444422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)