FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58750
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA2722346.33702127659575No Hit
CATAGATCCAGCTTCATCCATGTCCCGTGGTGTGCAAATTCTCTATGTGT22303.795744680851064No Hit
CATAGAAAGGGACAAGGGCTCACTCTAGCCTGGGAGACATCTATGTGTAG18483.145531914893617No Hit
CATAGAAAGGGACAAGGGCTCACTCTTTGGGTTCTATGTGTAGAACCATG11341.9302127659574466No Hit
CATAGAACCAACCCTGATTCTGCTGCTCACTCTTTGGGTTCTATGTGTAG9281.5795744680851063No Hit
CATAGAACAATCAGGCACCCACCATGCTGAAGTCCCATGATCTATGTGTA8141.385531914893617No Hit
CATAGAGGCGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA8111.3804255319148937No Hit
CATAGATCAAGGTGTGAGCATGGTCCCGTGGTGTGCAAATTCTCTATGTG5390.9174468085106383No Hit
CATAGACTCACTCTGTCACCCAAGCTGGAGTGCAGTGGCGTGCTCTTGGC4750.8085106382978723No Hit
CATAGATCTCAAACAGCCTCTCCCAGGAAAGTTGGAACCTATCAGTGTCT4000.6808510638297872No Hit
CATAGACTCACTCTGTCACCCAAGCTGGAGTGCAGTGGCATGACCTTAGC3880.6604255319148936No Hit
CATAGAGCCATCATCATTTCTCACCCAGGAAAGTTGGAACCTATCAGTGT3780.6434042553191489No Hit
CATAGATGTTGTTGTTCTGTTGCCCAATATGAACAGAAAGAAGCTCTATG3230.5497872340425531No Hit
CATAGACTCACTCTGTCACCCAAGCTGGAGTGCAGTGGCTCAAACTCGGC2920.4970212765957447No Hit
CATAGATGAAAGAGCATTGGTTGTAACACCGAAAAACACCCTCTATGTGT2880.4902127659574468No Hit
CATAGAAAGGGACAAGGGCTCACTCTGCTGCTCACTCTTTGGGTTCTATG2640.4493617021276596No Hit
CATAGATGTTGTTGTTCTGTTCCCCAATATGAACAGAAAGAAGCTCTATG2580.4391489361702128No Hit
CATAGAGTTCTCACTCTCCGCTAATTCTGTGTGGCCAACAGGAAGGTCTA2420.41191489361702127No Hit
CATAGAACAATCAGGCACCCACCATGTCGTCAGTGTAGAAGCTTTGAGAT2320.39489361702127657No Hit
CATAGATCCAGCTTCATCCATGTCCCGTGGTGTGCAAAATTCTCTTGACA2240.38127659574468087No Hit
CATAGACTCACTCTGTCACCCAAGCTGGAGTGCAGTGGTGTGATCATATC2220.3778723404255319No Hit
CATAGAACAATCAGGCACCCACCATGTCGTCAGTGTAGTATGCAGTCTGT2190.3727659574468085No Hit
CATAGATGTTGTTGTTCTGTTGCCCAGGAAAGTTGGAACCTATCAGTGTC1890.32170212765957445No Hit
CATAGATAGCAGCAATCTCAGGCCTCTATAGGTAAACAAACTTCTGGCCC1860.31659574468085105No Hit
CATAGATGAAAGAGCATTGGTTGTGACACCGAAAAACACCCTCTATGTGT1840.31319148936170216No Hit
CATAGAACAATCAGGCACCCACCATGTCGTCAGTGTACCAGGAAAGTTGG1800.30638297872340425No Hit
CATAGATAGCAGCAATCTCAGGCCTGGGAGACCCCATATCTACCAAAGAT1760.29957446808510635No Hit
CATAGATGCCAAGAAGAGAAAAGCAGCCATTGTCGCCTTCTCTTCTCTAT1750.2978723404255319No Hit
CATAGAGCTAGATCTAATTCTGTATGAGCACATACGGTAGCAGGAAAGTT1740.29617021276595745No Hit
CATAGACTCACTCTGTCACCCAAGCTATAGACTCCTCTTCTTCCTCCTCC1640.27914893617021275No Hit
CATAGATCCCTGGCAAGAATTCCTCTTCTTCCTCCTCCTGTCTATGTGTA1600.2723404255319149No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGGTCCGCGCAGAGCCTTCAGGTCA1440.2451063829787234No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA1380.2348936170212766No Hit
CATAGACTCACTCTGTCACCCAAGCTAGAAGAGAGGCAAGGGTACTATTT1350.2297872340425532No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA1310.2229787234042553No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG1300.22127659574468087No Hit
CATAGAAAGGGACAAGGGCTCACTCTCTGCTGCTCACTCTTTGGGTTCTA1300.22127659574468087No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA1290.2195744680851064No Hit
CATAGATCTCAAACAGCCTCTCCCAGAAGCTTTGAGATTTGTGTCTGTTC1270.21617021276595744No Hit
CATAGAACTGAAACTAGGAAACATGTCTGAGTGTAGGTAAACAAACTTCT1220.20765957446808508No Hit
CATAGAGCCATCATCATTTCTCACCCAGGGTAATTCTGGCCTTGTTCTAT1200.20425531914893616No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCACAGAGCCTTCAGGTCA1180.20085106382978724No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA1150.19574468085106383No Hit
CATAGAACAATCAGGCACCCACCATGTGTCAGTGTACTATGGCTTAATGT1130.19234042553191488No Hit
CATAGATGGTAAGATGACATAGTGAAGAGTCATGTCAGGAAAGTTGGAAC1090.18553191489361703No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGTCCGCGCAGAGCCTTCAGGTCAGA1080.18382978723404256No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGCCA1050.17872340425531913No Hit
CATAGAAAGGGACAAGGGCTCACTCTTTGGGTTCTGCTGCTCACTCTTTG1030.1753191489361702No Hit
CATAGATGTTGTTGTTCTGTTGCCCAGGTTGGAGTGCAATGACATGATCA1000.1702127659574468No Hit
CATAGATCCCTGGCAAGAATTCCTCACACTGAAACATTTTCTGGTCAGCT1000.1702127659574468No Hit
CATAGATCAAGGTGTGAGCATGGTCCCGTGGTGTGCAAAATTCTCTTGAC920.15659574468085108No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCGGAGCCTTCAGGTCA900.15319148936170213No Hit
CATAGAGGTGAGCGGCTGTCCCCACAAGTCCGCGCAGAGCCTTCAGGTCA900.15319148936170213No Hit
CATAGAACTGAAACTAGGAAACATGTCTGAGGCTGAAACTCTAAGGGTCT890.15148936170212765No Hit
AATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA880.14978723404255317No Hit
CATAGAACAATCAGGCACCCACCATGTCGTCAGTGTACCAAAAGCACAAG870.14808510638297873No Hit
CATAGAACAATCAGGCACCCACCATGTCGTCAGTGTAACCTGTGTATATT870.14808510638297873No Hit
CGTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA820.13957446808510637No Hit
CATAGATCTCAAACAGCCTCTCCCAGGGTAATTCTGGCCTTGTTCTATGT810.13787234042553193No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGGGCCTTCAGGTCA770.13106382978723402No Hit
CATAGAGCCATCATCATTTCTCACCCAGAGGTAAACAAACTTCTGGCCCT760.12936170212765957No Hit
CATAGAGATCTTCTCGGCTTAGCGGCTGTCTCCACAAGTCCGCGCAGAGC760.12936170212765957No Hit
CATAGAAAGGGACAAGGGCTCACTCTTTGGGTTCTATGTGTAGAACTGCT740.12595744680851062No Hit
CATAGATCCCTGGCAAGAATTCCTCATGGTGGGAGTATACTGATAGCTCT720.1225531914893617No Hit
CATAGAGATCTTCTCGGCTTAGCGGCCATTGTCGCCTTCTCTTCTCTATG710.12085106382978723No Hit
CATAGAGGTGAGCGGCTGCCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA710.12085106382978723No Hit
CATAGATCCAGCTTCATCCATGTCCCTGAAAAGAACACGAATGAGGAAGA680.11574468085106383No Hit
CATAGAACAATCAGGCACCCACCATGTCGCAGGAAAGTTGGAACCTATCA680.11574468085106383No Hit
CATAGAGCTAGATCTAATTCTGTATGAGCACAAGGGAGAATCTATGTGTA680.11574468085106383No Hit
CATAGACACAACGTTTGAGACTGCCTGTGACACCGAAAAACACCCTCTAT670.11404255319148937No Hit
CATAGATCTAGCTTCATCCATGTCCCGTGGTGTGCAAATTCTCTATGTGT660.1123404255319149No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGTGCAGAGCCTTCAGGTCA650.11063829787234043No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGCCCGCGCAGAGCCTTCAGGTCA650.11063829787234043No Hit
CATAGATCCAGCTTCATCCATGTCCCAGTGTACCAAAAGCACAAGGGAGA650.11063829787234043No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGCGCCTTCAGGTCA630.10723404255319148No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGTAGAGCCTTCAGGTCA630.10723404255319148No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)