FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457973
Sequences flagged as poor quality0
Sequence length150
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG6861914.983197699427695No Hit
CATAGAGAATTTGCACACCACGGGACATGGATGAAGCTGGATCTATGAGA426309.308409011011566No Hit
CATAGATGTCTCCCAGGCTAGAGTGAGCCCTTGTCCCTTTCTATGAGACC280526.125251925331843No Hit
CATAGATCATGGGACTTCAGCATGGTGGGTGCCTGATTGTTCTATGAGAC155413.393431490502715No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGAGAGGCTGTTTGAGATCT146303.1945114668331978No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAATCAGGGTTGGTTCTATGAGACC109792.3973028977690825No Hit
CATAGAGAATTTGCACACCACGGGACCATGCTCACACCTTGATCTATGAG106842.332888620071489No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGTGAGAAATGATGATGGCT104592.2837590862343413No Hit
CATAGAACAAGGCCAGAATTACCCTGGGTGAGAAATGATGATGGCTCTAT88251.9269694938347892No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGCAACAGAACAACAACATCTATG84941.854694490723252No Hit
CATAGACCTTCCTGTTGGCCACACAGAATTAGCGGAGAGTGAGAACTCTA74251.621274616625871No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGCAACAGAACAACAACATC72071.573673557174768No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACATGGGCAAC67941.483493568398137No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGGAACAGAACAACAACATCTATG66571.4535791411284071No Hit
CATAGAGGGTGTTTTTCGGTGTTACAACCAATGCTCTTTCATCTATGAGA64391.405978081677304No Hit
CATAGAACAAGGCCAGAATTACCCTGGGAGAGGCTGTTTGAGATCTATGA63521.386981328593607No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAGTGAGCCCTTGTCCCTTTCTATG49721.0856535210591018No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGTGCTCATACAGAATTAGATC46821.0223310107801113No Hit
CATAGAGCTATCAGTATACTCCCACCATGATCTGAGCAAGGATAGCCCTC44000.9607553283708865No Hit
CATAGAGGGTGTTTTTCGGTGTCACAACCAATGCTCTTTCATCTATGAGA41830.913372622403504No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACCACGGGACATGGATGAAGC38900.8493950516733519No Hit
CATAGACAGCTGACCAGAAAATGTTTCAGTGTGAGAGGTCTTGGCAGGCT37890.8273413498175657No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCAGCAGAATCAGGGTTGGTTCT28510.6225257820875904No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCCAGGCCTGAGATTGCTGCTAT28400.6201238937666631No Hit
CATAGAGCAAAAGAGAAACATTAAGCCATAGTACACTGACGACATGGTGG25090.547848890655126No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTGGGAGAGGCTGTTTGAGATC23330.5094186775202905No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTCTGACGACATGGGCAACAGA22720.4960991150133305No Hit
CATAGACTTGATGCCCAGGAAACCTCTCTGAGACCAAGACTTCAGTTGAC21510.4696783434831311No Hit
CATAGACCACACCCAAACACCACGTACACTGACGACATGGTGGGTGCCTG21330.4657479807761593No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGACACTGACGACATGGTGGGT21320.4655296272924386No Hit
CATAGAGGGCCAGAAGTTTGTTTACCTACACTGACGACATGGTGGGTGCC20840.45504866007384714No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAGAGTGAGCCCTTGTCCCTTTCTA20770.4535201856878026No Hit
CATAGACCACACCCAAACACCACGTAGGACCATGCTCACACCTTGATCTA20600.44980817646455135No Hit
CATAGATGTCTCCCAGGCTAGAGTGCGCCCTTGTCCCTTTCTATGAGACC19720.4305930698971337No Hit
CATAGACTTGATGCCCAGGAAACCTCGACATGGTTCTACACATAGATTAG19170.4185836282924976No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACCACGGGACCATGCTCACAC18920.41312479119948126No Hit
CATAGACTTGATGCCCAGGAAACCTCACACTGACGACATGGTGGGTGCCT18140.39609321946927No Hit
CATAGATCTGTGGCTCCCGATAAATGTGCTCATACAGAATTAGATCTAGC17280.3773148198692936No Hit
CATAGACAGCTCACAGACTGCATACTACACTGACGACATGGTGGGTGCCT16470.3596281876879205No Hit
CATAGAATGCAGGAGAATATACACAGGTTACACTGGACCATGCTCACACC16090.35133075530653557No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACATGGTGGGT15950.34827380653444634No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAACCCAAAGAGTGAGCCCTTGTCC15400.33626436492981027No Hit
CATAGACAGGAGGAGGAAGAAGAGGAATTCTTGCCAGGGATCTATGAGAC15340.33495424402748636No Hit
CATAGATTAGTTCTTATTCTTGGTATGGCACCGTATCAGACATGTTTCCT15010.3277485790647047No Hit
CATAGACCACACCCAAACACCACGTACACTGACGACATGGTTCTACACAT14990.32731187209726337No Hit
CATAGAATCAAAATAGTACCCTTGCCTCTGCTACCGTACTGGGTGAGAAA14630.31945114668331975No Hit
CATAGACAGCTCACAGACTGCATACTACACTGGAGACAGCCGCTCACCTC13850.3024195749531086No Hit
CATAGAACCCAAAGAGTGAGCCCTTGTCCCTTTCTATGAGACCAAGTCTC13440.29346708212056166No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCCCAAAGAGTGAGCCCTTGTCCCT13270.28975507289731056No Hit
CATAGATTCTCCCTTGTGCTTTTGGTGTTACAACCAATGCTCTTTCATCT13110.28626141715778003No Hit
CATAGACAGCTGACCAGAAAATGTTTCAGACATGTTTCCTAGTTTCAGTT12800.2794924591624397No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCACTGACGACATGGTTCTACACAT12260.2677013710415243No Hit
CATAGACAGCTCACAGACTGCATACTACACTGGGGACATGGATGAAGCTG12150.26529948272059706No Hit
CATAGATTCTCCCTTGTGCTTTTGGTACACTGACGACATGGTGGGTGCCT11620.2537267480834023No Hit
CATAGAACAAGGCCAGAATTACCCTGCATAGAGGCTGCTTTTCTCTTCTT11460.2502330923438718No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAAGAGTGAGCCCTTGTCCCTTTCT11140.2432457808648108No Hit
CATAGATTCTCCCTTGTGCTTTTGGTGTTGGAGACAGCCGCTCACCTCTA11100.2423723669299282No Hit
CATAGAGAATTTGCACACCACGGGACATGGATGAAGCTGGACCTATGAGA11100.2423723669299282No Hit
CATAGATTAGTTCTTATTCTTGGTATGGCACTATGAGACCAAGCTGGGAG10920.23844200422295636No Hit
CATAGAGAAGAGAAGGCGACAATGGAGACAGCCGCTCACCTCTATGAGAC9980.21791677675321472No Hit
CATAGAGCAAAAGAGAAACATTAAGCCATAGTACACTCAGACATGTTTCC9170.2002301445718416No Hit
CATAGATTCTCCCTTGTGCTTTTGGTGTATGTGTAGGGACATGGATGAAG8210.1792682101346586No Hit
CATAGAACCCAAAGAGTGAGCAGCAGTTCTACACATAGAACCCAAAGAGT7970.17402772652536286No Hit
CATAGATGTCTCCCAGGCTAGAGTGAGCCCTTGTCCCTTTCCATGAGACC7400.16158157795328545No Hit
CATAGAACCCAAAGAGTGAGCAGCAGATGGGCAACAGAACAACAACATCT6840.14935378286492873No Hit
CATAGAACCCAAAGAGTGAGCAGCAGGAACCCAAAGAGTGAGCCCTTGTC6840.14935378286492873No Hit
CATAGAACCCAAAGAGTGAGCAGCAGTAGAACCCAAAGAGTGAGCCCTTG6470.14127470396726446No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCCAGGCTAGAGTGAGCCCTTGT6470.14127470396726446No Hit
CATAGACAGGAGGAGGAAGAAGAGGAGGGACATGGATGAAGCTGGATCTA6350.13865446216261657No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCTCACTCAGTATCCCATGTTCC6300.13756269474401328No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGCATCAAGGGGTGGTCTTA6190.13516080642308606No Hit
CATAGATCATGGGACTTCAGCATGGCCGCTAAGCCGAGAAGATCTCTATG6180.13494245293936544No Hit
CATAGAACCCAAAGAGTGAGCAGCAGGTTCTACACATAGAACCCAAAGAG6070.13254056461843822No Hit
CATAGAGAAGAGAAGGCGACAATGGCTCTGCTACCTCGGCAGTGAGGTAC5990.13079373674867295No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCATAGAACCCAAAGAGTGAGCCCT5760.12577160662309786No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCCGCG5720.12489819268821525No Hit
CATAGAACCCAAAGAGTGAGCAGCAGACCCAAAGAGTGAGCCCTTGTCCC5570.1216228904324054No Hit
CATAGATTAGTTCTTATTCTTGGTATGGCACACTGACGACATGGTGGGTG5490.11987606256264015No Hit
CATAGAACCCAAAGAGTGAGCAGCAGGTGGGCAACAGAACAACAACATCT5470.11943935559519885No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGACACTGACGGCATGGTGGGT5140.1122336906324172No Hit
CATAGATCATGGGACTTCAGCATGGCTGCTTTTCTCTTCTTGGCATCTAT5120.1117969836649759No Hit
CATAGACCCTTAGAGTTTCAGCCTCAGACATGTTTCCTAGTTTCAGTTCT4940.10786662095800406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)