FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84712
Sequences flagged as poor quality0
Sequence length150
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA3568242.12154122202285No Hit
CATAGATTGCACATAAAGTCTGATCCCATCTCAAGTCTCACTCTTCTATG42074.9662385494380965No Hit
CATAGACTACCACTACCCAGCACAGCTACTTCTCTATGCACTTTGCCACA18712.2086599301161582No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGTGATTACAGGTG16471.9442345830578904No Hit
CATAGACTACCACTACCCAGCACAGGAGCACCCTCTATGTGTAGAACCAT14871.7553593351591272No Hit
CATAGAAGCAGCATTGATCATCCTCCACCCCATCCTACATGTGTCTATGT13201.5582207951647937No Hit
CATAGACCATCCTCCCACCTTGACCTCCTGAGTAGCTGAGACCACAGGCA13021.5369723297761828No Hit
CATAGAGCCTGGCATTTGAGGTAAGGCTTGTGAGGGCTGAAGTCTATGTG12451.4696855227122485No Hit
CATAGAGATGTCAAGAGCAATGCCCAGCCACCCCATCCTACATGTGTCTA8631.018745868353952No Hit
CATAGACTACCACTACCCAGTACAGGAGCACCCTCTATGTGTAGAACCAT8270.9762489375767305No Hit
CATAGACCATCTGCGCATCCTGTCTGACATGTAGGATGGGTTTTTGCCAG7050.8322315610539239No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAGGTGTAAGGGAAGCCAC5710.67404854093871No Hit
CATAGATGACATAGACCTTATACCCTCATCAAAAACTAATCCCAGAGCAC5610.6622438379450373No Hit
CATAGACTACCACCACCCAGTACAGGAGCACCCTCTATGTGTAGAACCAT5540.6539805458494664No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGTG5380.6350930210595901No Hit
CATAGACCATCCTCCCACCTTGACCTCTTAAGTAGCTGGGACTACAGGTG4490.5300311644159033No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAAGTGAAGGAGAGGCCAC4330.5111436396260269No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTATTACTACAGGTG3690.4355935404665219No Hit
CATAGACCATCCTCCCACCTTGACCTCCCGAGTAGCTGGGACTACAGAAG3550.4190669562753801No Hit
CATAGACTCAAGAAACTTACAATCATGGCACTTCAATGCTGTTTGTCTTC3470.40962319388044194No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAGGCAAAGGGGGAGCAGA3370.3978184908867693No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAGAGTGCTGGAATTATAGGCG3050.3600434413070167No Hit
CATAGAGATGTCAAGAGCAATGCCCACCCCATCCTACATGTGTCTATGTG2920.3446973274152422No Hit
CATAGAAGCAGCATTGATCATCCTCCAAGCAGCATTTTAACACAGCATTG2850.33643403531967137No Hit
CATAGACCATCCTCCCACCTTGACCTCCCACCTCCTGAGTAGCTGGGATT2750.32462933232599867No Hit
CATAGAGATGTCAAGAGCAATGCCCATGCTGAAGTCCCATGATCTATGTG2730.3222683917272642No Hit
CATAGAAGCAGCATTGATCATCCTCCAGCAGCATTTTAACACAGCATTGA2650.312824629332326No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGTCCGCGCAGAGCCTTCAGGTCAGA2570.30338086693738786No Hit
CATAGACCATCCTCCCACCTTGACCTCCATCTTGACCTCCCAAAGTGCTG2480.29275663424308246No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGCG2340.2762300500519407No Hit
CATAGACCATCCTCCCACCTTGACCTATCCTCCCATCTCAAGTCTCACTC2280.2691472282557371No Hit
CATAGACCATCCTCCCACCTTGACCTTCCAAAGTGCTAGGATAATAGGTG2100.24789876286712628No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA2070.24435735196902444No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCACAGAGCCTTCAGGTCA1970.23255264897535177No Hit
CATAGACCATCCTCCCACCTTGACCTCCTGAGTAGCTGGGACTACAGGCA1960.2313721786759845No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGGGCCTTCAGGTCA1930.22783076777788272No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCGGAGCCTTCAGGTCA1810.21366512418547548No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAACAGGTACTGGGATTACAGG1810.21366512418547548No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGCCCGCGCAGAGCCTTCAGGTCA1770.20894324298800643No Hit
CATAGAGGTGAGCGGCTGTCTCCATCTCCCAGGTGCAAGCGATTCTCCTG1720.20304089149117008No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG1710.2018604211918028No Hit
CATAGAGATGTCAAGAGCAATGCCCATGCCTAGCCATTTTTGGTTCTATG1670.19713853999433376No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAGTAACTGGGACTACAGGTG1650.19477759939559922No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAGTAGCTGGGACCACAGGTG1630.19241665879686468No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGGTCCGCGCAGAGCCTTCAGGTCA1580.18651430730002833No Hit
CATAGAAGGTGATAATCATGACAGCCACCCCATCCTACATGTGTCTATGT1540.18179242610255927No Hit
CATAGAAGGTGATAATCATGACAGCCAACTGATCTTTGACAAAGCAAACA1530.18061195580319198No Hit
CATAGAGATGTCAAGAGCAATGCCCAGCCACATTATTTTCTATGTGTAGA1520.17943148550382473No Hit
CATAGAGATGTCAAGAGCAATGCCCGGCCACATTATTTTCTATGTGTAGA1500.1770705449050902No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGCCA1490.17589007460572292No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGAG1490.17589007460572292No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA1480.17470960430635565No Hit
CATAGAAGCAGCATTGATCATCCTCCACCCAGTACAGGAGCACCCTCTAT1470.17352913400698838No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGGGCTGGGATGACACGCA1460.1723486637076211No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA1430.1688072528095193No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAGGCAAAAAAGAAGCAGG1420.16762678251015206No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGCA1390.16408537161205022No Hit
CATAGATCCCAGGCTTTAGAAAATATACCACCACCCAGTACAGGAGCACC1350.15936349041458117No Hit
CATAGACCATCCTCCCACCTTGACCTCTCAAAGTGCTAGGGTTACAGGCA1320.15582207951647936No Hit
CATAGAAGCAGCATTGATCATCCTCCACCAATGCCATCCAGCTCTGAGCC1320.15582207951647936No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGTAGAGCCTTCAGGTCA1320.15582207951647936No Hit
CATAGAGGTGAGCGGCTGTCTCCACACGTCCGCGCAGAGCCTTCAGGTCA1270.149919728019643No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGAGATTACACACG1270.149919728019643No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA1270.149919728019643No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAGGCTCAGGTTATTCTCCCA1220.1440173765228067No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCTGCGCAGAGCCTTCAGGTCA1190.14047596562470488No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTAGGATTACAAGTG1160.13693455472660307No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAGGTGAACTGGTGGCCAG1130.13339314382850126No Hit
CATAGACCATCCTCCCACCTTGACCTCATCCTCCCATCTCAAGTCTCACT1080.12749079233166494No Hit
CATAGAGTTTTGTTCCCTTGCTGGCAGTCTCATAGACTACCACCACCCAG1040.12276891113419586No Hit
CATAGACCATCCTCCCACCTTGACCTTCCCGGTAGCTGGGACTACAGGCA980.11568608933799225No Hit
CATAGAGTTTTGTTCCCTTGCTGGCAAGGAGTTATGATCCTTTGGAGGAG980.11568608933799225No Hit
CATAGACCATCCTCCCACCTTGACCTCCTGAGTAGCTGGGACTGTGCATG980.11568608933799225No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGTATTACAGGTG970.114505619038625No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGCGCCTTCAGGTCA940.11096420814052319No Hit
CATAGAGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA940.11096420814052319No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGTTAGGATTACAGGCA910.10742279724242138No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAGGGCGAAGGGGAAGCAGG860.10152044574558504No Hit
CATAGACCATCTGCGCATCCTGTCTGAGAAGTGAGGAGCGTCTCCGCCCG860.10152044574558504No Hit
CATAGACCATCTGCGCATCCTGTCTGTCCTCTACTTGGCTCTCCCTCCGC860.10152044574558504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)