FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33164
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA1561847.093233626824265No Hit
CATAGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA20676.232661922566638No Hit
CATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG8502.563020142323001No Hit
CATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG6351.9147268122060066No Hit
CATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT4921.4835363647328428No Hit
CATAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC4881.4714750934748522No Hit
CATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG2830.8533349415028344No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTACGGTAGCAAAGCTG2700.814135809914365No Hit
CATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG2690.8111204920998674No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTTACGGTAGCAAAGCT1650.49752743939211197No Hit
CATAGATACGATCATAAGGGTGGGGCTGGGCTCAGTGGCTTATGCCTGTA1600.4824508503196237No Hit
CATAGACTTTTCCTCCAATATAGCTGCACTTAATCTATGTGTAGCCATCA1400.42214449402967075No Hit
CATAGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1370.41309854058617773No Hit
CATAGACACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1250.376914726812206No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGCAGAGACTTGGTCTCATAGAG1240.37389940899770835No Hit
CATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1170.3527921842962248No Hit
CATAGATGTGAGCACTACTGATAATCCCTGATGTTTTAAAATGAGCACCT1150.3467615486672295No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGGTCCGCGCAGAGCCTTCAGGTCA970.2924858280062719No Hit
CATAGACCTGACCTCATGATCTGCCTACCTCCGCCTCCCAAAGTGCTGGG890.26836328549029065No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTGTACGGTAGCAAAGC880.26534796767579305No Hit
CATAGATGATGTGAAAAGGACCCATGGCGTCAGTGTACCAGTGTAGCAGA810.2442407429743095No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA810.2442407429743095No Hit
CATAGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA800.24122542515981185No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGAGACTTGGTCTCATAGAGGGG770.2321794717163189No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA770.2321794717163189No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGCCA770.2321794717163189No Hit
CATAGACTTACTGCCCTCACTCCGTCAGTGTAGATTTATCCAGCATGCCC760.22916415390182127No Hit
CATAGATGACCTGTTAATCTGCCCCCTTGCTTCCTAGGGAAAGCTGTCTG750.2261488360873236No Hit
CATAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT750.2261488360873236No Hit
CATAGATGTGAGCACTACTGATAATCCCTGATGTGGCATGGAGAAGGGTC740.22313351827282596No Hit
CATAGATGACCTGTTAATCTGCCCCCAGATCACAGAGTGGCCTTGGGCAA710.214087564829333No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA680.20504161138584007No Hit
CATAGACTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC660.19901097575684476No Hit
CATAGACTTACTGCCCTCACTCCGTCTGCATAGCCACATCTATGTGTAGA660.19901097575684476No Hit
CATAGAGTCTCAATTATTCATCTCAACATGCTGAAGTCCCATGATCTATG640.19298034012784948No Hit
CATAGAGGGGAGACACTGGCTATAAGAACAGAGACTTGGTCTCATAGAGG630.18996502231335183No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGCCCGCGCAGAGCCTTCAGGTCA610.18393438668435652No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT570.17187311542636594No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA570.17187311542636594No Hit
CATAGAGGGGAGACACTGGGGGCACAAAGTTCTATGTGTAGAACCATGTC560.1688577976118683No Hit
CATAGAGGGGAGACACTGGCTATAAGAACTTGGTCTCATAGAGGGGAGAC560.1688577976118683No Hit
CATAGACCAGCTTTGCATGAGTGTGGCAGACCATCTTCTATGTGTACCAG560.1688577976118683No Hit
CATAGACCTGACCTCATGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGG560.1688577976118683No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGACACTGGGGGCACAAAGTTCT530.15981184416837535No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCGGAGCCTTCAGGTCA520.1567965263538777No Hit
CATAGACCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG510.15378120853938004No Hit
CATAGAGTCTCAATTATTCATCTCAACATGCTGAAGTGCAAAGCTGCTAT510.15378120853938004No Hit
CATAGACTTACTGCCCTCACTCAGTCTGCATAGCCACATCTATGTGTAGA510.15378120853938004No Hit
CATAGATGTTTGAAACTCCTCTACCTAAGATTTATCCAGCATGCCCCTCT500.15076589072488242No Hit
CATAGAGTCTCAATTATTCATCTCAACATGCTACTTGGCCTCAGTTTCCC500.15076589072488242No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG500.15076589072488242No Hit
CAGTTTCCCCTCCTTCTATGTGTAGAACCATGTCGTCAGTGTAGATCTCG490.14775057291038474No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGATCA480.1447352550958871No Hit
CATAGAGGTGAGCGGCTGTCTCCAGAAGTCCGCGCAGAGCCTTCAGGTCA470.14171993728138946No Hit
CATAGATTAGGGGTGACGGACTAGCCCGCATTGGGTCAACAGTATAGAAC470.14171993728138946No Hit
CATAGATTAGGGGTGACGGACTAGCCACTTGGCCTCAGTTTCCCCTCCTT470.14171993728138946No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGACTTGGTCTCATAGAGGGGAG460.13870461946689183No Hit
CATAGATGACCTGTTAATCTGCCCCCGTACCATCAATTTAAGGTAGGCAC460.13870461946689183No Hit
CATAGATTAGGGGTGACGGACTAGCCTCCCATCCTACTGCTGACCAGCCC450.13568930165239415No Hit
CATAGACCAGCTTTGCATGAGTGTGGCATGGAGAAGGGTCTATGTGTAGA450.13568930165239415No Hit
CATAGATGATGTGAAAAGGACCCATGGCCTCAGTTTCCCCTCCTTCTATG430.12965866602339887No Hit
CATAGACCAAACACAGAAGACATGGCAGGGGAGACACTGGGGGCACAAAG410.12362803039440357No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTACGGTAGCAAAGCTGCTATGGGA400.12061271257990593No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTTGGCCTCAGTTTCCCCTCCTTCT400.12061271257990593No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCTGCGCAGAGCCTTCAGGTCA400.12061271257990593No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGTCCGCGCAGAGCCTTCAGGTCAGA400.12061271257990593No Hit
CATAGACTTTTCCTCCAATATAGCTGCACTATAGATTTATCCAGCATGCC390.11759739476540827No Hit
CATAGACTTACTGCCCTCACTCCGTCACCCAGGCTGGAGTCCAGTAGCGT390.11759739476540827No Hit
CATAGACACCTTGTATATGCCAGGACTGACACCTAGTGATTACCTGGGTG390.11759739476540827No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCAAGCAGATCTGTGTTCTATGTGTA380.11458207695091063No Hit
CATAGACCTGACCTCATGATCTGCCTGCCTCACCTTCCCAAAGTGCTGGG380.11458207695091063No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCACAGAGCCTTCAGGTCA370.11156675913641298No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGGGCCTTCAGGTCA370.11156675913641298No Hit
CATAGATGATGTGAAAAGGACCCATGGGAAAGCTGTCTGTGTAGGTGATC360.10855144132191533No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTTCCCTTGCCCTGTGGCCCTGGCT360.10855144132191533No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGAGGGGAGACACTGGGGGCACA360.10855144132191533No Hit
CATAGACCTGACCTCATGATCTGCCTACCTCAGCCTCCCAGATTGCTGGG360.10855144132191533No Hit
CATAGACCTGACCTCATGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGG360.10855144132191533No Hit
CATAGACCTCCTTTCCTCCACGGCAAAGCTGCTATGGGATCTTCTATGTG350.10553612350741769No Hit
CATAGACCTGACCTCATGATCTGCCTGCCTTGGCCTCCCAAAATGCTGGG350.10553612350741769No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)