FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125523
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA72625.785393911872724No Hit
NNTAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA71695.7113039044637235No Hit
NNNNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA69145.508153884148722No Hit
NATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA68585.4635405463540545No Hit
NNTNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA56234.479657114632378No Hit
NNNNGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA19081.5200401520040152No Hit
NNNAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA18001.4340001434000142No Hit
NNNAGNGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA17681.4085068075173475No Hit
NNTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA17621.4037268070393474No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA15551.2388167905483458No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA14891.186236785290345No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA13761.0962134429546777No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA13751.0954167762083442No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA10880.8667734200106754No Hit
NATAGAGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA10590.8436700843670084No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT6310.5026967169363383No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT6260.4987133832046717No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT6230.4963233829656717No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT6100.48596671526333823No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT5820.46366004636600466No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT5380.4286067095273376No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG5040.40152004015200404No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG4650.3704500370450037No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT4610.3672633700596703No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT4570.364076703074337No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT4480.3569067023573369No Hit
NNNNGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA4340.3457533679086701No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG4340.3457533679086701No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT4010.3194633652796699No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG3850.3067166973383364No Hit
NNNAGNATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3830.30512336384566974No Hit
NNNAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3600.28680002868000287No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT3230.2573233590656692No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG3210.25573002557300256No Hit
NNTAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3200.25493335882666923No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT3080.2453733578706691No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA3070.2445766911243358No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2800.22306668897333556No Hit
NNNAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2720.21669335500266884No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2690.2143033547636688No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC2630.20952335428566876No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2630.20952335428566876No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2590.20633668730033541No Hit
NNTAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2540.20235335356866868No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC2530.20155668682233532No Hit
NNNNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2480.19757335309066865No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2470.1967766863443353No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2430.19359001935900194No Hit
NATAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2330.18562335189566853No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC2300.1832333516566685No Hit
NNTNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2290.18243668491033516No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG2230.17765668443233512No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG2200.17526668419333508No Hit
NNNAGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA2120.16889335022266835No Hit
NATAGAATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA2120.16889335022266835No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2070.16491001649100165No Hit
NNNAGNCCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG2020.16092668275933494No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1990.15853668252033493No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1940.15455334878866822No Hit
NNTAGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1930.1537566820423349No Hit
NNTAGNCCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG1900.15136668180333485No Hit
NATAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1900.15136668180333485No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1870.14897668156433483No Hit
NNTNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1790.14260334759366808No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1750.13941668060833473No Hit
NNNAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1720.1370266803693347No Hit
NNNNGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1690.13463668013033467No Hit
NNNNGNCCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG1680.13384001338400134No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1670.13304334663766798No Hit
NNNNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1670.13304334663766798No Hit
NNNAGNCTGCCTCAGNCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1590.12667001266700129No Hit
NNNAGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG1590.12667001266700129No Hit
NATAGACCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG1560.12428001242800124No Hit
NNTAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1450.11551667821833447No Hit
NNNNGACTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG1430.1139233447256678No Hit
NNTAGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG1410.11233001123300113No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCCTGCTAT1360.10834667750133442No Hit
NATAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG1350.10755001075500109No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCACC1310.1043633437696677No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCCTGCTAT1300.10356667702333437No Hit
NNNAGNATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1280.10197334353066768No Hit
NNNNGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1280.10197334353066768No Hit
NNNAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1260.10038001003800101No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)