FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112164
Sequences flagged as poor quality0
Sequence length150
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGCTTGGGTGCGAACATCAAGGAAGTTTCTGTGAGATCTATGAGA1193210.637994365393531No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGTAATAACCAGGCCCCTCTATGAG65725.85927748653757No Hit
CATAGACGGATTATTCTCTCCATGGTCTACCAGTTATTAGTTGAATGAGC58065.17634891765629No Hit
CATAGAGAAGACTCCCTCAACCCACCCACAGCCAGGAAAGAATCTATGAG54264.837559288185157No Hit
CATAGAAATGATGCTGAAGGGCCTGCCCTCTCTGCTGTTCCTTCTATGAG40733.6312898969366283No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGTTCTGGGCCCCAGTGTGAA37513.34421026354267No Hit
CATAGACTCATTGCACTTTCCACAACTGTGTGCTTGAACCAGATTCTATG33482.9849149459719695No Hit
CATAGACTCATTGCACTTTCCACAACTGTCATTGTCGCTGTCCCATCTAT27602.460682571948219No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTCTTCCTTTTCTGCCTTCATCTA26592.3706358546414177No Hit
CATAGATCATGGGACTTCAGCATGGCTCAGGTATGTCTTTATTAGCTCTA23812.1227844941335903No Hit
CATAGACCAGCAGACACTCCCATCATTTATTTTAAAGGACTGGAAGCCTC23762.118326735851075No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAGGAACCATGGGAGGTCTAT23582.1022788060340214No Hit
CATAGATCACGTAGAAACTGGAGAGGCTAATCTGAAATCTTCGTGTTCTA19571.7447665917763275No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAAGGAAGTTTCTGTGAGATC18541.6529367711565208No Hit
CATAGATCAAGGAAAACACCAGCCCACGCTGTCCTTCTCTGAATCTATGA15501.3819050675796156No Hit
CATAGACTCATTGCACTTTCCACAACTGTCCCAACTGTCTCTGAAAAATT14661.3070147284333655No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGAAGTGATGGGCTAGATCTATGA14211.2668949038907313No Hit
CATAGACCAACATTTCTTCTCCCAGGGTGGAAGGGTAAGAGAGGGTCTAT13761.2267750793480974No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTGCCTCTAAGTAAACTGAAT12911.1509931885453442No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGGGGTAAGGCGTACTGGTCTATGA12661.12870439713277No Hit
CATAGATGTTTTATCAAAGGCATGCTCATTTATTTTAAAGGACTGGAAGC12491.1135480189722193No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGCCCCAGTGTGAATACTTCT11221.000320958596341No Hit
CATAGACGTGACTAAAAGCATGTTGGTGGAAGGGTAAGAGAGGGTCTATG9350.8336007988302842No Hit
CATAGAAATGATGCTGAAGGGCCTGTTTCCCCACTCAAATACCATCTATG9350.8336007988302842No Hit
CATAGAAGCTGAGAAGACTTCCTAATGCAGAAGTAGGAAGTATTACCCCT9220.8220106272957455No Hit
CATAGAGAAGACTCCCTCAACCCACGCTGTCCTTCTCTGAATCTATGAGA8550.7622766663100461No Hit
CATAGACCAGCAGACACTCCCATCATCAAGGAAGTTTCTGTGAGATCTAT6440.5741592667879176No Hit
CATAGAAGCCATGAACACAGGATTGAGGAGCTGAGTCATGGGGTCTATGA6180.5509789237188403No Hit
CATAGAAGGACAAAGACATTTAGGACCCACGCTGTCCTTCTCTGAATCTA5970.5322563389322778No Hit
CATAGACCAGCAGACACTCCCATCATCAGGAACCATGGGAGGTCTATGAG5680.5064013408936914No Hit
CATAGAAAATTTGTGATGGTAAATCATGGGACATGCCAGGAATCAATGTT5380.47965479119860205No Hit
CATAGAGTCTGAATGGTGGGAGAGTGAAAGAAATCTACAAAGGATTCTGG4940.440426518312471No Hit
CATAGATCACGTAGAAACTGGAGAGGCCAAGTCACGCTATACCACACTAC4820.4297278984344353No Hit
CATAGAGCTGTACTGCATCTTTTCTCAAACTGCTGGGGTTGCTCTATGAG4660.4154630719303876No Hit
CATAGAGTCCAACTATTCCAGCACCAACACTGACGATCATTGTTCACTGC4610.41100531364787274No Hit
CATAGACCTCCGACAGTTTTTATGCACCTTCATTGTTGCTCTATGAGACC4570.40743910702186087No Hit
CATAGATGTGTAGCCTGGGTCTGACATGCCAGGAATCAATGTTCTATGAG4280.3815841089832745No Hit
CATAGACCGCTTCCCAATTTTTGTAACACACTGACGCTATACCACACTAC3820.3405727327841375No Hit
CATAGACGTGACTAAAAGCATGTTGGTCCTCTCTTACCCTTCCCCACACC3700.3298741129061018No Hit
CATAGAGTCCAACTATTCCAGCACCATGTCGGGTGGAAGGGTAAGAGAGG3560.31739238971506006No Hit
CATAGACCTAAAAGGCTATTGAAAGGAAGGCTAATCTGAAATCTTCGTGT3280.2924289433329767No Hit
CATAGAAGGACAAAGACATTTAGGACCCAAGGGGTAAGGCGTACTGGTCT3280.2924289433329767No Hit
CATAGACCCAAGACCCCAGCCTTTACACTGACGCTATACCACACTACACA3210.28618808173745586No Hit
CATAGAGTCCAACTATTCCAGCACCAGAGATCCCACCATTTGCCATCTAT3110.2772725651724261No Hit
CATAGACACAACAGAGAGAACGGGCTGGTGTCTCAAACTGCTGGGGTTGC3060.2728148068899112No Hit
CATAGACATTTAATCCTCACAATGACCCATGCTAGAGATCCCACCATTTG2990.2665739452943904No Hit
CATAGACCCAAGACCCCAGCCTTTACACACTGACGCTATACCACACTACA2970.26479084198138436No Hit
CATAGACGTGACTAAAAGCATGTTGGTCTGTTTCCCCACTCAAATACCAT2900.2585499803858636No Hit
CATAGACCAGCAGACACTCCCATCATACACTGACGCTATACCACACTACA2860.25498377375985165No Hit
CATAGACCTCCGACAGTTTTTATGCACTGTCCCAACTGTCTCTGAAAAAT2850.2540922221033487No Hit
CATAGAGTGCCTGTTTCCTCATTCGTGTCTGTTTCCCCACTCAAATACCA2840.2532006704468457No Hit
CATAGAAAAAACAGTTGGCCAGGCATGTCTGAGGAGAATTCAAGCTGCTC2830.2523091187903427No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTACACTGACGATCATTGTTCACT2480.22110481081273847No Hit
CATAGAAATGATGCTGAAGGGCCTGCGATGCTGAAGTGATGGGCTAGATC2450.21843015584322956No Hit
CATAGATGGCCAAGATACGAGCACATACACTGACGACATGGTTCTACACA2420.2157555008737206No Hit
CATAGAAATGATGCTGAAGTGATGGGCTAGATCTATGAGACCAAGTCTCT2390.2130808459042117No Hit
CATAGAGTCTGAATGGTGGGAGAGTGATAGGCTTAGAGGGCTTGCTCTAT2350.2095146392781998No Hit
CATAGAAATGATGCTGAAGGGCCTGCACATAGAAATGATGCTGAAGTGAT2310.20594843265218787No Hit
CATAGATCAAGGAAAACACCAGCCCAGACACTCCCAAGTATTAGAGCAAG2280.20327377768267893No Hit
CATAGATGTTTTATCAAAGGCATGCTCATACACTGACGCTATACCACACT2270.20238222602617595No Hit
CATAGACCAACATTTCTTCTCCCAGGGTCTGTTTCCCCACTCAAATACCA2240.19970757105666703No Hit
CATAGACCTCCGACAGTTTTTATGCACCCACAGCCAGGAAAGAATCTATG2180.19435826111764914No Hit
CATAGAGTCCAACTATTCCAGCACCACACTGCCCTCTCTGCTGTTCCTTC2130.18990050283513427No Hit
CATAGATGTTTTATCAAAGGCATGCTCATACACTGCCCTCTCTGCTGTTC2120.18900895117863128No Hit
CATAGAGTAGTTAAAACCCACAATGACAGTGTTGCTGAAGTGATGGGCTA2080.18544274455261936No Hit
CATAGAAGGACAAAGACATTTAGGACCCACGCTATACCACACTACACACT2080.18544274455261936No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACTCCTGTCATTGTCGCTGTCC1970.17563567633108662No Hit
CATAGATGTTTTATCAAAGGCATGCTCATATGCTGAAGTGATGGGCTAGA1950.17385257301808069No Hit
CATAGACCTAAAAGGCTATTGAAAGGAAGGGGTGGAAGGGTAAGAGAGGG1920.17117791804857174No Hit
AAGGATTCTGGGCCATCTATGAGACCAAGTCTCTGCTACCGTAGTCTAGC1790.159587746514033No Hit
CATAGAAGGACAAAGACATTTAGGACCCACAAGGGGTAAGGCGTACTGGT1730.15423843657501515No Hit
CATAGACATTTAATCCTCACAATGACCCATTGCTTCTTGTGGTAACTGGT1730.15423843657501515No Hit
CATAGAAATGATGCTGAAGGGCCTGCGAAATGATGCTGAAGTGATGGGCT1730.15423843657501515No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACTGCTGGGGTTGCTCTATGAG1700.15156378160550624No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGCTGAAGTGATGGGCTAGATCTA1680.14978067829250025No Hit
CATAGAAATGATGCTGAAGGGCCTGCTACCGTACTGCCCTCTCTGCTGTT1620.14443136835348241No Hit
CATAGAAGCTGAGAAGACTTCCTAATGCACACTGACGACATGGTTCTACA1570.1399736100709675No Hit
CATAGAGTGCCTGTTTCCTCATTCGTCAGTGTCATTGCTTCTTGTGGTAA1550.13819050675796157No Hit
CATAGACATTTAATCCTCACAATGACCCATAGAGATCCCACCATTTGCCA1550.13819050675796157No Hit
CATAGAGTGTCTGTTTCCCCACTCAAATACCATCTATGAGACCAAGTCTC1540.13729895510145856No Hit
CATAGATTGTGTGTCTCTTCTATATTAACAGTCACACTGACGACATGGTT1520.13551585178845263No Hit
CATAGATCAAGGAAAACACCAGCCCACATAGAGTGTCTGTTTCCCCACTC1500.13373274847544667No Hit
CATAGAAGGACAAAGACATTTAGGACCCAGTCATTTATTTTAAAGGACTG1480.13194964516244073No Hit
CATAGACCAACATTTCTTCTCCCAGGGTGTTCTACACATAGAGTGTCTGT1450.1292749901929318No Hit
CATAGATCAAGGAAAACACCAGCCCAACACTGACGACATGGTTCTACAAT1450.1292749901929318No Hit
CATAGACCAGCAGACACTCCCATCATGTTTCTCATTTATTTTAAAGGACT1430.12749188687992583No Hit
CATAGACATTTAATCCTCACAATGACCCATGTCACAAATGAGAAACCTGG1420.12660033522342284No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGACATGCCAGGAATCAATGT1400.1248172319104169No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTCTGAAAAATTTCTATGAGA1340.11946792197139902No Hit
CATAGATTGTGTGTCTCTTCTATATTAACAGTCTGTTTCCCCACTCAAAT1330.11857637031489605No Hit
CATAGAAATGATGCTGAAGGGCCTGCACACTGACGACATGGTTCTACACA1330.11857637031489605No Hit
CATAGATCATGGGACTTCAGCATGGAGACAGCCGCTCACCTCTATGAGAC1310.11679326700189008No Hit
CATAGAAAAAACAGTTGGCCAGGCATCAATGTTCTATGAGACCGAGTCTC1290.11501016368888413No Hit
CATAGAAATGATGCTGAAGGGCCTGGATGCTGAAGTGATGGGCTAGATCT1250.11144395706287223No Hit
CATAGAAGGACAAAGACATTTAGGACCCACACTGACGACATGGTTCTACA1210.1078777504368603No Hit
CATAGAAATGATGCTGAAGGGCCTGTGCTGAAGTGATGGGCTAGATCTAT1200.10698619878035734No Hit
CATAGAGAAGACTCCCTCAACCCACCTGATGCTGAAGTGATGGGCTAGAT1180.10520309546735139No Hit
CATAGACATTTAATCCTCACAATGACCCATGATTCCTCCCAAGTATTAGA1180.10520309546735139No Hit
CATAGAGAAGACTCCCTCAACCCACCATCAAGGGGTAAGGCGTACTGGTC1160.10341999215434541No Hit
CATAGAAAATTTGTGATGGTAAATCATGGGAGGGGTGGAAGGGTAAGAGA1160.10341999215434541No Hit
CATAGAGAAGACTCCCTCAACCCACCATACGCTATACCACACTACACACT1140.10163688884133946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)