FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36731
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGACCTGGGCTTAGGGGCTATACATGGCTATGCAATCTATGAGACCA21385.820696414472789No Hit
CATAGAACTTCACAAGGGCTTTAGGGGCTACACATGGCTATGCAATCTAT20405.553891808009583No Hit
CATAGATTCTGAAGAAATCAGATAGAGAGAAACTATGATCTATCCAGGAA19745.374207073044567No Hit
CATAGACCTGGGCTTAGGGGCTACACATGGCTATGCAATCTATGAGACCA18735.099234978628407No Hit
CATAGAGCCAAGGAAAGTTGAATGGAGACAGCCGCTCACCTCTATGAGAC17644.8024829163376985No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGGGCAAGCAATGACAG15024.089188968446272No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGGGGAAAGGATTCTAT13543.6862595627671446No Hit
CATAGACCTTTGGGTTCATATCATCTGGAGACAGCCGCTCACCTCTATGA10232.785113391957747No Hit
CATAGATCCCATCCAATAATAATGAACAAGCAATGGGGAAAGGATTCTAT8232.240614195094062No Hit
CATAGATGCCCTTTTTGTGTACTTCCACACTGACGACATGGTTCTACACA7972.1698292995017834No Hit
CATAGATGCCCTTTTTGTGTACTTCCACATGGTTCTACACATAGATGCCC7482.0364269962701806No Hit
CATAGACAATTAAATTTTCTTAAGGGGTTGCACAGATCATTTAAGCTCAA6611.7995698456344775No Hit
CATAGAAACCCCAAAGGCTATGTTCAAAATGGTCAAACAGGAGGTCTATG6421.7478424219324278No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTTCCTTCTGCTGGCTATTCTATG5621.5300427431869539No Hit
CATAGACCTGGGCTTAGGGGCTATACGTGAATGTGGATCAGAACTTCTAT4341.1815632571941956No Hit
CATAGACAGGGAGGCGTATAAGTGATGTACCATGTTTTCATCCTGCTTCT4311.1733957692412402No Hit
CATAGATCATGGGACTTCAGCATGGAGACAGCCGCTCACCTCTATGAGAC4101.1162233535705535No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTATCCCCAGAAATGTTAGCATGT3851.048160953962593No Hit
CATAGATGCCCTTTTTGTGTACTTCCACACTGACTGATACAGGAAGGCCT3480.9474286025428112No Hit
CATAGATCCTCTCATTGTCCAGTTTGGACGGTTGCACAGATCATTTAAGC3420.9310936266369008No Hit
CATAGAGAGATTTTGGGCTGAGACGAATCCTTTCCCTCACTCTCTCCATC3360.9147586507309902No Hit
CATAGAGATGTTTGATAAGCAGGGTGATCCTTTCCCTCACTCTCTCCATC3140.8548637390759849No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTTAGCATGTCTATGAGAC3110.8466962511230296No Hit
CATAGATTTGTAAATTGCCTAGTCTTGCATACACTGACTGATACAGGAAG2980.8113038033268901No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCTATGTCTGTTATTCAAAGGTT2860.778633851515069No Hit
CATAGAACCTCTTTTGGGGCAAAGTTCAATAAATGGGGGCAAGGAAGTCT2300.6261740763932373No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTTATCCCCAGAAATGTTA2210.6016716125343715No Hit
CATAGACCTTTGGGTTCATATCATCTGGCTACACATGGCTATGCAATCTA2200.5989491165500531No Hit
CATAGATGCCCTTTTTGTGTACTTCCACACTGGAGACAGCCGCTCACCTC2150.585336636628461No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTACACTGACGACATGGTTCTACA2030.5526666848166398No Hit
CATAGATGCCCTTTTTGTGTACTTCCATGTGCTTCTTTCAAAAGTCTGGT1550.4219868775693556No Hit
CATAGATGCCCTTTTTGTGTACTTCCAGATGCCCTTTTTGTGTGCTTCTT1350.3675369578829871No Hit
CATAGATCATGGGACTTCAGCATGGCTACACATGGCTATGCAATCTATGA1250.3403119980398029No Hit
CATAGACCTTTGGGTTCATATCATCTGGATGGAGCAGAATTACCTCTGGA1180.32125452614957395No Hit
CNTAGACCTGGGCTTAGGGGCTATACATGGCTATGCAATCTATGAGACCA1120.30491955024366335No Hit
CATAGATCCCATCCAATAATAATGAGCAGAATTACCTCTGGATGGTCTAT1120.30491955024366335No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGACAGTCTATGAGACC1100.29947455827502656No Hit
CNTAGAACTTCACAAGGGCTTTAGGGGCTACACATGGCTATGCAATCTAT1080.2940295663063897No Hit
CATAGAACTTCACAAGGGCTTTAGGGGCTATACGTGAATGTGGATCAGAA1070.29130707032207126No Hit
CATAGAGGATCCCATCTGAGTTGGTGTTGATTGTGTTATTGGTAAGGGTC1070.29130707032207126No Hit
CNTAGATTCTGAAGAAATCAGATAGAGAGAAACTATGATCTATCCAGGAA1000.2722495984318423No Hit
CATAGATGTCCATCATGTGGAAGCTGTCACAGACAAGCAATGGGGAAAGG990.2695271024475239No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTATCCCCAGAAATGTTAG980.2668046064632055No Hit
CATAGATGAAAGCCTGTTGAATTTATGAGACCAAGTCTCTGCTACCGTAC980.2668046064632055No Hit
CATAGATCCCATCCAATAATAATGAGCAATGGGCAAGCAATGACAGTCTA950.2586371185102502No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCCCGTAGTGTGCTTCTTTCAAA950.2586371185102502No Hit
CATAGAACTTCACAAGGGCTTTAGGGCTACACATGGCTATGCAATCTATG950.2586371185102502No Hit
CNTAGAGCCAAGGAAAGTTGAATGGAGACAGCCGCTCACCTCTATGAGAC930.2531921265416133No Hit
CATAGACAGAAACCACCCAGACAACCTGATTGGCCATTTGAACTCTATGA900.24502463858865806No Hit
CATAGATGCCCTTTTTGTGTACTTCCACTGACGACATGGTTCTACACATA900.24502463858865806No Hit
CATAGAGCTGGCTTCCTAACTTCCAACAATGGGCAAGCAATGACAGTCTA890.2423021426043397No Hit
CNTAGACCTGGGCTTAGGGGCTACACATGGCTATGCAATCTATGAGACCA790.21507718276115545No Hit
CATAGATGCCCTTTTTGTGTACTTCCACACTGACGACATGGTTCTACATA730.19874220685524488No Hit
CATAGAGCAACAGGGACTCTCATTCAAAGGTTTTCTCTGTTATTCAAAGG720.19601971087092646No Hit
CNTAGATCCCATCCAATAATAATGAGCAAGCAATGGGCAAGCAATGACAG700.19057471890228964No Hit
CATAGAGCCAAGGAAAGTTGAATGGAGGCAAAATAAATGGGGGCAAGGAA690.18785222291797118No Hit
CNTAGATCCCATCCAATAATAATGAGCAAGCAATGGGGAAAGGATTCTAT690.18785222291797118No Hit
CATAGAGCTGGCTTCCTAACTTCCAAACAATGGGCAAGCAATGACAGTCT620.16879475102774222No Hit
CATAGATGTATACAAGCTGTCACAGACAACAATGGGCAAGCAATGACAGT610.1660722550434238No Hit
CATAGATCCTCTCATTGTCCAGTTTGGCTACACATGGCTATGCAATCTAT590.16062726307478697No Hit
CATAGATGTCCATCATGTGGAAGCTGCTTCCTTCTGCTGGCTATTCTATG560.15245977512183168No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAACAATGGGCAAGCAATGA500.13612479921592116No Hit
CNTAGATGCCCTTTTTGTGTACTTCCACACTGACGACATGGTTCTACACA480.1306798072472843No Hit
CATAGAACTTCACAAGGGCTTTAGGGTACGGTAGCAGAATTACCTCTGGA480.1306798072472843No Hit
CATAGAGCTGGCTTCCTAACTTCCAACGGTAGCAGAATTACCTCTGGATG470.1279573112629659No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGACAATGGGCAAGCAA470.1279573112629659No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTACCATGTTTTCATCCTG450.12251231929432903No Hit
CATAGATGCCCTTTTTGTGTGCTTCTTTCAAAAGTCTGGTCTATGAGACC430.1170673273256922No Hit
CNTAGACCTTTGGGTTCATATCATCTGGAGACAGCCGCTCACCTCTATGA430.1170673273256922No Hit
CATAGATGTCCATCATGTGGAAGCTGCTACCGTACCTTCCTAACCATGGG420.11434483134137377No Hit
CATAGATGCCCTTTTTGTGTACTTCCATGGTTCTACACATAGATGCCCTT410.11162233535705535No Hit
CNTAGATCCCATCCAATAATAATGAACAAGCAATGGGGAAAGGATTCTAT410.11162233535705535No Hit
CAAAGTCACGAAAGTGGGTCTATGAGACCAAGTCTCTGCTACCGTACTGA410.11162233535705535No Hit
CATAGATTGGAACTTTGCCAGCCTTACACTGACGACATGGTTCTACACAT390.10617734338841851No Hit
CATAGACAGAAACCACCCAGACAACCATGTCGTATACGTGAATGTGGATC380.10345484740410009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)