FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36731
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA3871.0536059459312297No Hit
NNTAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA3791.0318259780566825No Hit
NNTAGNTTTAAAATAAAATTCCTGGATAGATCATAGTTTCTCTCTATCTG3640.9909885382919059No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT3530.9610410824644033No Hit
NNNAGNTTTAAAATAAAATTCCTGGATAGATCATAGTTTCTCTCTATCTG3460.9419836105741745No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA3440.9365386186055376No Hit
NNTAGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA3390.9229261386839454No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT3300.8984236748250796No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT3290.8957011788407613No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA3230.8793662029348507No Hit
NNNAGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA3230.8793662029348507No Hit
NNTAGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG3220.8766437069505322No Hit
NNNNGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA3210.8739212109662139No Hit
NNNAGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG3210.8739212109662139No Hit
NNNNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA3170.8630312270289401No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT3080.8385287631700744No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT3050.830361275217119No Hit
NATAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2960.8058588113582532No Hit
NNTNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2950.8031363153739349No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2930.797691323405298No Hit
NNTNGNTTTAAAATAAAATTCCTGGATAGATCATAGTTTCTCTCTATCTG2910.7922463314366612No Hit
NNNNGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG2900.7895238354523427No Hit
NNNNGNTTTAAAATAAAATTCCTGGATAGATCATAGTTTCTCTCTATCTG2880.7840788434837058No Hit
NATAGATTTAAAATAAAATTCCTGGATAGATCATAGTTTCTCTCTATCTG2870.7813563474993875No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2710.7377964117502926No Hit
NNTNGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA2590.7051264599384716No Hit
NNNAGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA2570.6996814679698348No Hit
NATAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG2510.6833464920639242No Hit
NNNAGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT2490.6779015000952874No Hit
NNTAGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA2450.6670115161580137No Hit
NNTAGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT2440.6642890201736952No Hit
NATAGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA2410.65612153222074No Hit
NNTNGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG2350.6397865563148295No Hit
NNNNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA2320.6316190683618742No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2280.6207290844246005No Hit
NATAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT2190.5962266205657347No Hit
NATAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA2130.5798916446598241No Hit
NNNNGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1990.5417767008793662No Hit
NNTNGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1890.514551741036182No Hit
NNTNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1790.48732678119299777No Hit
NNTAGNGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT1770.4818817892243609No Hit
NNNAGNGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT1720.4682693093027688No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT1640.4464893414282214No Hit
NNNNGNGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT1540.4192643815850372No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT1450.39476191772617136No Hit
NATAGAGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT1440.3920394217418529No Hit
NATAGAATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT1370.372981949851624No Hit
NNTNGNGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT1320.3593694699300319No Hit
NNNAGNCCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG1270.34575699000843974No Hit
NNTAGNCCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG1270.34575699000843974No Hit
NNNAGNTTGAGCTTAAATGATCTGTGCAACCCCTTAAGAAAATTTAATTG1270.34575699000843974No Hit
NNTAGNTTGAGCTTAAATGATCTGTGCAACCCCTTAAGAAAATTTAATTG1260.34303449402412134No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT1080.2940295663063897No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT1060.28858457433775286No Hit
NNNNGNATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG1060.28858457433775286No Hit
NNNNGNCCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG1050.2858620783534344No Hit
NNNNGNTTGAGCTTAAATGATCTGTGCAACCCCTTAAGAAAATTTAATTG1040.283139582369116No Hit
NNNNGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA990.2695271024475239No Hit
NNNAGNTTGCATAGCNATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA980.2668046064632055No Hit
NNTAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA980.2668046064632055No Hit
NNNNGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG970.2640821104788871No Hit
NNNAGNTTTAAAATANAATTCCTGGATAGATCATAGTTTCTCTCTATCTG940.2559146225259318No Hit
NNNNGATTTAAAATAAAATTCCTGGATAGATCATAGTTTCTCTCTATCTG940.2559146225259318No Hit
NNTAGNATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG940.2559146225259318No Hit
NATAGATTGAGCTTAAATGATCTGTGCAACCCCTTAAGAAAATTTAATTG920.25046963055729493No Hit
NNNAGNTTGCATAGCNATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT920.25046963055729493No Hit
NNTNGNCCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG910.24774713457297648No Hit
NNNAGNATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG910.24774713457297648No Hit
NNTNGNTTGAGCTTAAATGATCTGTGCAACCCCTTAAGAAAATTTAATTG900.24502463858865806No Hit
NATAGACCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG880.23957964662002124No Hit
NNNAGATTTAAAATAAAATTCCTGGATAGATCATAGTTTCTCTCTATCTG880.23957964662002124No Hit
NNTAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA870.2368571506357028No Hit
NNNNGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA860.2341346546513844No Hit
NNNAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA860.2341346546513844No Hit
NNNAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA850.23141215866706596No Hit
NNTAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT830.22596716669842912No Hit
NNNAGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT830.22596716669842912No Hit
NNNNGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA830.22596716669842912No Hit
NNNNGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT820.2232446707141107No Hit
NNTAGNGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA820.2232446707141107No Hit
NNNAGNAAGGCCTTCCTGTATCAGTCAGTGTGGAAGTACACAAAAAGGGC820.2232446707141107No Hit
NNTAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG810.22052217472979227No Hit
NNNAGNGGTGAGCGGNTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA810.22052217472979227No Hit
NNNAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT800.21779967874547385No Hit
NNNNGNAGCAGGATGAAAACATGGTACATCACTTATACGCCTCCCTGTCT790.21507718276115545No Hit
NNTAGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT780.21235468677683703No Hit
NNTAGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA770.2096321907925186No Hit
NNNAGNGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA770.2096321907925186No Hit
NATAGAATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG770.2096321907925186No Hit
NNTAGNAGCAGGATGAAAACATGGTACATCACTTATACGCCTCCCTGTCT770.2096321907925186No Hit
NNNAGNCCAGACTTTNGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG760.20690969480820018No Hit
NNNAGNAGCAGGATGAAAACATGGTACATCACTTATACGCCTCCCTGTCT750.20418719882388173No Hit
NNNNGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA750.20418719882388173No Hit
NNTNGNATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG740.2014647028395633No Hit
NNTAGATTTAAAATAAAATTCCTGGATAGATCATAGTTTCTCTCTATCTG740.2014647028395633No Hit
NNNAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG740.2014647028395633No Hit
NNNNGNGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA710.19329721488660806No Hit
NNNAGNTTGCATAGCNATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA700.19057471890228964No Hit
NNNAGNCTGTCATTGNTTGCCCATTGCTTGCTCATTATTATTGGATGGGA690.18785222291797118No Hit
NATAGAAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT680.18512972693365276No Hit
NNTAGNTTGAGCTTAAATGATCTGTGCAACCGTCCAAACTGGACAATGAG680.18512972693365276No Hit
NNTAGNAAGGCCTTCCTGTATCAGTCAGTGTGGAAGTACACAAAAAGGGC670.18240723094933434No Hit
NNNAGNTGGAGAGAGTGAGGGAAAGGATTCGTCTCAGCCCAAAATCTCTC670.18240723094933434No Hit
NNTAGNCATGCTAACATTTCTGGGGATACTGGGGACTGTGACTCTGTTTT660.17968473496501594No Hit
NATAGAAGCAGGATGAAAACATGGTACATCACTTATACGCCTCCCTGTCT660.17968473496501594No Hit
NNNNGNCATGCTAACATTTCTGGGGATACTGGGGACTGTGACTCTGTTTT650.17696223898069752No Hit
NNNNGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT650.17696223898069752No Hit
NNNAGNCATGCTAACATTTCTGGGGATACTGGGGACTGTGACTCTGTTTT650.17696223898069752No Hit
NNNAGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA640.1742397429963791No Hit
NNTNGNAGCAGGATGAAAACATGGTACATCACTTATACGCCTCCCTGTCT640.1742397429963791No Hit
NATAGATGGAGAGAGTGAGGGAAAGGATTCGTCTCAGCCCAAAATCTCTC630.17151724701206067No Hit
NNNNGNTGGAGAGAGTGAGGGAAAGGATCACCCTGCTTATCAAACATCTC610.1660722550434238No Hit
NNNNGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT610.1660722550434238No Hit
NNTAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA610.1660722550434238No Hit
NNTNGNTGGAGAGAGTGAGGGAAAGGATCACCCTGCTTATCAAACATCTC600.1633497590591054No Hit
NNTAGNTGGAGAGAGTGAGGGAAAGGATTCGTCTCAGCCCAAAATCTCTC600.1633497590591054No Hit
NNTAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT600.1633497590591054No Hit
NNNNGNAAGGCCTTCCTGTATCAGTCAGTGTATGCAAGACTAGGCAATTT590.16062726307478697No Hit
NNTAGNTGGAGAGAGTGAGGGAAAGGATCACCCTGCTTATCAAACATCTC590.16062726307478697No Hit
NNNNGNTGGAGAGAGTGAGGGAAAGGATTCGTCTCAGCCCAAAATCTCTC580.15790476709046855No Hit
NATAGAGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA580.15790476709046855No Hit
NNNAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA570.15518227110615013No Hit
NNNAGNTGGAGAGAGTGAGGGAAAGGATCACCCTGCTTATCAAACATCTC570.15518227110615013No Hit
NNNNGNTTGAGCTTAAATGATCTGTGCAACCGTCCAAACTGGACAATGAG570.15518227110615013No Hit
NNTAGNAAGGCCTTCCTGTATCAGTCAGTGTATGCAAGACTAGGCAATTT560.15245977512183168No Hit
NNNAGNTTGAGCTTAAATGATCTGTGCAACCGTCCAAACTGGACAATGAG550.14973727913751328No Hit
NNNNGAGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT550.14973727913751328No Hit
NATAGACATGCTAACATTTCTGGGGATACTGGGGACTGTGACTCTGTTTT540.14701478315319486No Hit
NNTNGNTTGAGCTTAAATGATCTGTGCAACCGTCCAAACTGGACAATGAG530.14429228716887643No Hit
NNNNGNAAGGCCTTCCTGTATCAGTCAGTGTGGAAGTACACAAAAAGGGC530.14429228716887643No Hit
NNNNGNGCAGAAAACCTTTGAATAACAGACATAGAGCAGGAATTAGACTC530.14429228716887643No Hit
NNNAGNGGTGAGCGGNTGTCTCCAGATGATATGAACCCAAAGGTCTATGT520.141569791184558No Hit
NNTAGNGCAGAAAACCTTTGAATAACAGACATAGAGCAGGAATTAGACTC520.141569791184558No Hit
NNNAGNATCCTTTCCNCATTGCTTGCTCATTATTATTGGATGGGATCTAT520.141569791184558No Hit
NATAGATTTAAAATANAATTCCTGGATAGATCATAGTTTCTCTCTATCTG510.13884729520023958No Hit
NNTAGAGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT510.13884729520023958No Hit
NNNAGNAAGGCCTTCCTGTATCAGTCAGTGTATGCAAGACTAGGCAATTT510.13884729520023958No Hit
NNTNGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT510.13884729520023958No Hit
NNNAGAGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT510.13884729520023958No Hit
NNNAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT500.13612479921592116No Hit
NATAGATTGAGCTTAAATGATCTGTGCAACCGTCCAAACTGGACAATGAG480.1306798072472843No Hit
NNNAGNGCAGAAAACCTTTGAATAACAGACATAGAGCAGGAATTAGACTC480.1306798072472843No Hit
NNTAGNCTTCCTTGCCCCCATTTATTGAACTTTGCCCCAAAAGAGGTTCT470.1279573112629659No Hit
NNTNGNTGGAGAGAGTGAGGGAAAGGATTCGTCTCAGCCCAAAATCTCTC470.1279573112629659No Hit
NNNAGNGGTGAGCGGCTGTCTCCAGTGTGGAAGTACACAAAAAGGGCATC470.1279573112629659No Hit
NNTNGNAAGGCCTTCCTGTATCAGTCAGTGTGGAAGTACACAAAAAGGGC460.12523481527864747No Hit
NATAGAGCAGAAAACCTTTGAATAACAGACATAGAGCAGGAATTAGACTC450.12251231929432903No Hit
NNTNGNCATGCTAACATTTCTGGGGATACTGGGGACTGTGACTCTGTTTT450.12251231929432903No Hit
NNNNGNCATGCTAACATTTCTGGGGATAACATTACTGGGGACTGTGACTC450.12251231929432903No Hit
NNTNGNGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA440.11978982331001062No Hit
NNTNGNAAGGCCTTCCTGTATCAGTCAGTGTATGCAAGACTAGGCAATTT430.1170673273256922No Hit
NNNAGAATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT430.1170673273256922No Hit
NNNAGNCATGCTAACATTTCTGGGGATAACATTACTGGGGACTGTGACTC420.11434483134137377No Hit
NATAGATTGCATAGCNATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA420.11434483134137377No Hit
NATAGAAAGGCCTTCCTGTATCAGTCAGTGTGGAAGTACACAAAAAGGGC410.11162233535705535No Hit
NNNAGNTTGCATAGCCATGTGTAGCCAGATGATATGAACCCAAAGGTCTA410.11162233535705535No Hit
NNTAGAATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT400.10889983937273692No Hit
NNNNGNGGTGAGCGGCTGTCTCCAGTGTGGAAGTACACAAAAAGGGCATC400.10889983937273692No Hit
NATAGATTGCATAGCCATGTGTAGCCAGATGATATGAACCCAAAGGTCTA390.10617734338841851No Hit
NATAGACTTCCTTGCCCCCATTTATTGAACTTTGCCCCAAAAGAGGTTCT380.10345484740410009No Hit
NATAGAAAGGCCTTCCTGTATCAGTCAGTGTATGCAAGACTAGGCAATTT380.10345484740410009No Hit
NATAGAGGTGAGCGGNTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA370.10073235141978165No Hit
NATAGAGGTGAGCGGCTGTCTCCAGTGTGGAAGTACACAAAAAGGGCATC370.10073235141978165No Hit
NATAGATTGCATAGCNATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT370.10073235141978165No Hit
NATAGACCAGACTTTNGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG370.10073235141978165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)