FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322662
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA7524023.318519069490677No Hit
CATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG195286.052153646850264No Hit
CATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG146774.548722812106787No Hit
CATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT121743.7729884523123265No Hit
CATAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC105883.2814524177002564No Hit
CATAGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA92932.880103637862531No Hit
CATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG87552.7133656891731905No Hit
CATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG87522.7124359236600526No Hit
CATAGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG55591.7228554958439481No Hit
CATAGACACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG42321.3115892171994223No Hit
CATAGATGATGTGAAAAGGACCCATGGCGTCAGTGTACCAGTGTAGCAGA37571.1643763442859711No Hit
CATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT36321.125636114571905No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCCAGAGACTTGGCCTCAGTTTCCCC29930.927596060273599No Hit
CATAGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA29630.918298405142223No Hit
CATAGAGGGGAGACACTGGGGGCACAAAGTTCTATGTGTAGAACCATGTC28540.8845169248315574No Hit
CATAGACTTACTGCCCTCACTCCGTCTGCATAGCCACATCTATGTGTAGA22600.7004233532303153No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGACACTGGGGGCACAAAGTTCT21810.6759395280510255No Hit
CATAGACTTTTCCTCCAATATAGCTGCACTGACTTGGCCTCAGTTTCCCC19330.5990789122983183No Hit
CATAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT19060.59071102268008No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT18780.5820332112241293No Hit
CATAGACTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC18670.5786240710092915No Hit
CATAGATGTTTGAAACTCCTCTACCTAAGAACTTGGCCTCAGTTTCCCCT18190.5637478227990901No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTACGGTAGCAAAGCTG17770.5507311056151639No Hit
CATAGATTAGGGGTGACGGACTAGCCTACGGTAGCAGACACTGGGGGCAC17760.5504211837774513No Hit
CATAGAGTCTCAATTATTCATCTCAACATGCTATAGATGATGTGGCATGG15890.49246580012520846No Hit
CATAGATGTTTGAAACTCCTCTACCTAAGATTTATCCAGCATGCCCCTCT15300.47418041170016917No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGGGGAGACACTGGGGGCACAAA14470.44845689917002934No Hit
CATAGAGGGGAGACACTGGCTATAAGAACTTGGTCTCATAGAGGGGAGAC14350.444737837117479No Hit
CATAGACCAGCTTTGCATGAGTGTGGCATGGAGAAGGGTCTATGTGTAGA14340.4444279152797664No Hit
CATAGACTTACTGCCCTCACTCAGTCTGCATAGCCACATCTATGTGTAGA13880.43017151074499005No Hit
CATAGACCTCCTTTCCTCCACGGCAAAGCTGCTATGGGATCTTCTATGTG13870.4298615889072776No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGAGGGGAGACACTGGGGGCACA12960.40165870167543744No Hit
CATAGACCAGCTTTGCATGAGTGTGGCCTCAGTTTCCCCTCCTTCTATGT11810.3660176903384966No Hit
CATAGATGATGTGAAAAGGACCCATGGGATGATGTGGCATGGAGAAGGGT11660.36136886277280866No Hit
CATAGACCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG10820.3353354284049563No Hit
CATAGATGTGAGCACTACTGATAATCCCTGATGTGGCATGGAGAAGGGTC10750.33316597554096855No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCAAGCAGATGAATGTTGGAGAGGCA10510.32572785143586785No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCAAGCAGATCTGTGTTCTATGTGTA10290.3189095710061922No Hit
CATAGATGATGTGAAAAGGACCCATGGCCTCAGTTTCCCCTCCTTCTATG9460.29318605847605234No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGCAGAGACTTGGTCTCATAGAG9390.29101660561206466No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTATCTTCAGAACGCGG8980.27830981026585094No Hit
CATAGATGTGAGCACTACTGATAATCCCTCTTGGCCTCAGTTTCCCCTCC8960.2776899665904259No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT8470.262503796542512No Hit
CATAGAGTCTCAATTATTCATCTCAACATGCTATAGAGGGGAGACACTGG8070.25010692303401083No Hit
CATAGATGTGAGCACTACTGATAATCCCTACGGTAGCAGAGCTTGGCCTC8040.24917715752087324No Hit
CATAGACTTTTCCTCCAATATAGCTGCACTTAATCTACCTCTCTCATAGC7700.23863981503864726No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCCTTGGCCTCAGTTTCCCCTCCTTC7330.22717270704328368No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGGGAGACACTGGGGGCACAAAG7300.2262429415301461No Hit
CATAGACACACCCAACTCTGCTCAGTCTACTCAGTCTGCATAGCCACATC7170.2222139576398832No Hit
CATAGAGTCTCAATTATTCATCTCAACATGCTAGGACTCAGTCTGCATAG7160.22190403580217066No Hit
CATAGAGGCTGGGTGACTTGGTGAATGTTGGAGAGGCACATCTATGTGTA6920.21446591169707002No Hit
CATAGATGATGTGAAAAGGACCCATGGCGTCAGTGTGCACACTACAATTA6870.21291630250850738No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTTGGCCTCAGTTTCCCCTCCTTCT6850.2122964588330823No Hit
CATAGAGTTATTCAGAGAGAGGGGAAATGAGAGAAAACTACCCCAGGCTC6490.20113927267543125No Hit
CAGTTTCCCCTCCTTCTATGTGTAGAACCATGTCGTCAGTGTAGATCTCG6480.20082935083771872No Hit
CATAGATGGTGGGTTCTGGAGAGAGGGCGAAAGTGATCAGAAGCTCTATG6380.19773013246059346No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCATCAATTTAAGGTAGGCACTTCCT6180.19153169570634285No Hit
CATAGAGGGGAGACACTGGCTATAAGAATAGAGGGGAGACACTGGGGGCA5680.17603560382071642No Hit
CATAGAAAGGCAATGTTCAAGTGGTTTTCTATGAGGGGCGAAAGTGATCA5130.15898990274652733No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGACTTGGTCTCATAGAGGGGAG4890.15155177864142663No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA4770.14783271658887628No Hit
CATAGACCAGCTTTGCATGATGTGGCATGGAGAAGGGTCTATGTGTAGAA4460.13822513961978788No Hit
CATAGACTTACTGCCCTCACTCCGTCTACGGTAGCAAACACTCAAAACAG4440.13760529594436283No Hit
CATAGAGGTGAGCGGCTGTCTCCATAAGTCCGCGCAGAGCCTTCAGGTCA4240.13140685919011225No Hit
CATAGATGACCTGTTAATCTGCCCCCACTCAGTCTGCATAGCCACATCTA4100.12706795346213684No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCTGCGCAGAGCCTTCAGGTCA4100.12706795346213684No Hit
CATAGACCAAACACAGAAGACATGGCAAAGCTGCTATGGGATCTTCTATG4090.1267580316244243No Hit
CATAGATTAGGGGTGACGGACTAGCCCTTGGCCTCAGTTTCCCCTCCTTC4010.12427865692272409No Hit
CATAGACCTGACCTCATGATCTGCCTACCTGACCTCTCTCATAGCAGCAT3960.12272904773416143No Hit
CATAGAACTTGCTCTCAAACTTCAGGGTGCTGATGTTTTAAAATGAGCAC3800.117770298330761No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG3700.11467107995363568No Hit
CATAGACCTGACCTCATGATCTGCCTACCTCCGCCTCCCAAAGTGCTGGG3660.11343139260278558No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGGAGACACTGGGGGCACAAAGT3590.11126193973879786No Hit
CATAGAGGTGAGCGGCTGTCTCCAGAAGTCCGCGCAGAGCCTTCAGGTCA3550.11002225238794777No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGCCA3550.11002225238794777No Hit
CATAGAGGTGAGCGGCTGTCTCCAGTGTAGACACTGGGGGCACAAAGTTC3550.11002225238794777No Hit
CATAGAGGGGAGACACTGGCTATAAGAACAGAGACTTGGTCTCATAGAGG3520.10909248687481017No Hit
CATAGATACGATCATAAGGGTGGGGCCATGCTGAAGTCCCATGATCTATG3450.10692303401082248No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTCTACACATAAAACCAAATTGAAC3330.10320397195827213No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA3290.101964284607422No Hit
CATAGAGGTGAGCGGCTGTCTCCAGGGCGAAAGTGATCAGAAGCTCTATG3280.10165436276970947No Hit
CATAGAGTCTCAATTATTCATCTCAACATGCCATGCTGAAGTCCCATGAT3240.10041467541885936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)