FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112526
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG13741.2210511348488349No Hit
NNTAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG13231.1757282761317385No Hit
NNNNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG12371.0993014947656543No Hit
NATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG11240.9988802587846365No Hit
NNTNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG10500.9331176794696336No Hit
NNTAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG9570.8504701135737518No Hit
NNNAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG9370.8326964434886159No Hit
NNNNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG8790.781152800241722No Hit
NNTAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT7750.6887297157990153No Hit
NATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG7700.6842862982777314No Hit
NNTNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG7590.6745107797309067No Hit
NNNAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT6950.6176350354584718No Hit
NNNNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT6550.5820876952882No Hit
NNTAGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT6210.551872456143469No Hit
NNNAGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT5870.5216572169987381No Hit
NNTAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG5600.49766276238380464No Hit
NNTNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT5600.49766276238380464No Hit
NATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT5600.49766276238380464No Hit
NNNAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG5410.4807777758029256No Hit
NNNAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG5380.47811172529015517No Hit
NNNNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA5360.47633435828164156No Hit
NNNNGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT5320.4727796242646144No Hit
NNNNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG5100.4532285871709649No Hit
NNNAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA5090.4523399036667082No Hit
NNTAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG5060.44967385315393776No Hit
NNTAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT4980.44256438511988344No Hit
NNTAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA4960.44078701811136983No Hit
NNNNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG4940.43900965110285617No Hit
NATAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT4910.43634360059008587No Hit
NNNAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT4910.43634360059008587No Hit
NNTNGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT4900.435454917085829No Hit
NATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA4740.42123598101772036No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC4680.4159038799921796No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC4640.41234914597515243No Hit
NATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG4530.4025736274283277No Hit
NATAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT4330.3847999573431918No Hit
NNTNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA4310.38302259033467817No Hit
NNNNGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT4300.3821339068304214No Hit
NNNNGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT4270.379467856317651No Hit
NATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG4250.37769048930913746No Hit
NNTAGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT4240.37680180580488065No Hit
NNNAGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT4140.3679149707623127No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC4090.36347155324102876No Hit
NNTNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG4080.36258286973677195No Hit
NATAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC4020.3572507687112312No Hit
NNTNGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT3870.34392051614737923No Hit
NNNAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3590.31903737802818904No Hit
NNTNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3590.31903737802818904No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3580.31814869452393224No Hit
NNNNGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3550.31548264401116183No Hit
NNTAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT3420.30392975845582354No Hit
NATAGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT3410.30304107495156674No Hit
NNTAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA3320.2950429234132556No Hit
NNTNGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT3310.2941542399089988No Hit
NNNNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT3170.2817126708494037No Hit
NNTAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3150.2799353038408901No Hit
NNNAGNCACACCCAACTCTGCTCAGTTCTTTTTGTTTGGCAGACACACAC3100.27549188631960614No Hit
NNNAGNCCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3080.27371451931109253No Hit
NNNAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT3000.2666050512770382No Hit
NATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2830.2514974317046727No Hit
NNNNGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2800.24883138119190232No Hit
NNNAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA2760.24527664717487516No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2730.24261059666210474No Hit
NNTNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2650.23550112862805042No Hit
NNNNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA2570.22839166059399604No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2440.21683877503865776No Hit
NNTNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA2440.21683877503865776No Hit
NNTAGNCACACCCAACTCTGCTCAGTTCTTTTTGTTTGGCAGACACACAC2360.20972930700460338No Hit
NNNNGNCACACCCAACTCTGCTCAGTTCTTTTTGTTTGGCAGACACACAC2340.2079519399960898No Hit
NNNNGNTGTGAGCACTACTGATAATCCCTTTCATAATGACTATTAGCTGA2340.2079519399960898No Hit
NNTAGNTGTGAGCACTACTGATAATCCCTTTCATAATGACTATTAGCTGA2320.20617457298757622No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2270.20173115546629222No Hit
NNNAGNTGTGAGCACTACTGATAATCCCTTTCATAATGACTATTAGCTGA2260.20084247196203547No Hit
NNTAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2250.19995378845777864No Hit
NATAGACACACCCAACTCTGCTCAGTTCTTTTTGTTTGGCAGACACACAC2220.19728773794500826No Hit
NATAGATGTGAGCACTACTGATAATCCCTTTCATAATGACTATTAGCTGA2180.1937330039279811No Hit
NNTAGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG2180.1937330039279811No Hit
NNNAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2150.1910669534152107No Hit
NNNAGNTACGATCATNAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2130.1892895864066971No Hit
NNNAGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG2110.18751221939818352No Hit
NATAGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2110.18751221939818352No Hit
NNTNGNCACACCCAACTCTGCTCAGTTCTTTTTGTTTGGCAGACACACAC2060.18306880187689958No Hit
NNTNGNTGTGAGCACTACTGATAATCCCTTTCATAATGACTATTAGCTGA2060.18306880187689958No Hit
NATAGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA2050.18218011837264275No Hit
NNNNGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT2040.18129143486838598No Hit
NNNNGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG2020.1795140678598724No Hit
NNTAGNCTGGCGGTGATTGCTTTCCTGATTTTGTCCTTTGTTTTTGGCAA2000.17773670085135881No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1930.17151591632156124No Hit
NNNAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1910.16973854931304766No Hit
NATAGACTGGCGGTGATTGCTTTCCTGATTTTGTCCTTTGTTTTTGGCAA1900.16884986580879086No Hit
NNNAGNCACCATTGGNGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1870.16618381529602047No Hit
NNTAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1830.1626290812789933No Hit
NNNNGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1770.15729698025345254No Hit
NNNNGNCTGGCGGTGATTGCTTTCCTGATTTTGTCCTTTGTTTTTGGCAA1710.15196487922791177No Hit
NNNAGNCTGGCGGTGATTGCTTTCCTGATTTTGTCCTTTGTTTTTGGCAA1690.1501875122193982No Hit
NNTAGNTGACCTGTTAATCTGCCCCCCCTCGGCCTCCCGAAGTGCTGAGA1670.1484101452108846No Hit
NNNNGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1630.14485541119385742No Hit
NATAGACACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1620.14396672768960062No Hit
NNNAGNTGACCTGTTAATCTGCCCCCCCTCGGCCTCCCGAAGTGCTGAGA1610.14307804418534384No Hit
NNTNGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1600.14218936068108703No Hit
NATAGACCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG1550.13774594315980307No Hit
NNNAGNGTTTGCCCTNCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1530.1359685761512895No Hit
NNNNGNTGACCTGTTAATCTGCCCCCCCTCGGCCTCCCGAAGTGCTGAGA1520.13507989264703268No Hit
NNNAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1510.13419120914277588No Hit
NNNAGNGATGATGGGATCTTATATTTGGAAAAACTTAACAACTCCACAAA1480.1315251586300055No Hit
NNNAGNTGGTGGGTTNTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1460.12974779162149191No Hit
NNNNGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA1450.12885910811723514No Hit
NNNAGNTTAGGGGTGACGGACTAGCCGACTTGGCCTCAGTTTCCCCTCCT1440.12797042461297833No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGCATGGAGAAGGGTCTATGTGTAGA1430.12708174110872153No Hit
NNTAGNGATGATGGGATCTTATATTTGGAAAAACTTAACAACTCCACAAA1420.12619305760446475No Hit
NNNNGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG1420.12619305760446475No Hit
NNTNGNCTGGCGGTGATTGCTTTCCTGATTTTGTCCTTTGTTTTTGGCAA1420.12619305760446475No Hit
NNNNGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1400.12441569059595116No Hit
NNNAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG1380.12263832358743758No Hit
NNTAGNGTTTGCCCTTCCTTCCCTCCAAGCAGATCTGTGTTCTATGTGTA1370.12174964008318079No Hit
NNTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA1370.12174964008318079No Hit
NNNNGNGATGATGGGATCTTATATTTGGAAAAACTTAACAACTCCACAAA1340.11908358957041039No Hit
NNTAGNCTTACTGCCCTCACTCCGTCTACGGTAGCAAAGCTGCTATGGGA1330.1181949060661536No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGCATGGAGAAGGGTCTATGTGTAGA1330.1181949060661536No Hit
NNNAGNGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA1300.11552885555338323No Hit
NNTAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1300.11552885555338323No Hit
NNNAGNGTTTGCCCTTCCTTCCCTCCCAGAGACTTGGCCTCAGTTTCCCC1300.11552885555338323No Hit
NNNAGNCCTGACCTCNTGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1290.11464017204912644No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGCATGGAGAAGGGTCTATGTGTAGA1290.11464017204912644No Hit
NNTAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG1290.11464017204912644No Hit
NNTAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1280.11375148854486962No Hit
NNTNGNTGACCTGTTAATCTGCCCCCCCTCGGCCTCCCGAAGTGCTGAGA1280.11375148854486962No Hit
NNNAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1260.11197412153635604No Hit
NATAGAGATGATGGGATCTTATATTTGGAAAAACTTAACAACTCCACAAA1250.11108543803209925No Hit
NNTAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1250.11108543803209925No Hit
NNNAGNGTTTGCCCTTCCTTCCCTCCAAGCAGATCTGTGTTCTATGTGTA1240.11019675452784246No Hit
NNNNGNTTAGGGGTGACGGACTAGCCGACTTGGCCTCAGTTTCCCCTCCT1240.11019675452784246No Hit
NNNAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1230.10930807102358567No Hit
NATAGATGACCTGTTAATCTGCCCCCCCTCGGCCTCCCGAAGTGCTGAGA1220.10841938751932888No Hit
NATAGATACGATCATNAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1210.10753070401507209No Hit
NNNAGNTGATGTGAAAAGGACCCATGGCTCTATGTGTGAGAGAAAACTAC1210.10753070401507209No Hit
NNNNGNGTTTGCCCTTCCTTCCCTCCAAGCAGATCTGTGTTCTATGTGTA1210.10753070401507209No Hit
NNTAGNCCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG1200.10664202051081527No Hit
NNNAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA1190.10575333700655848No Hit
NNTAGNTTAGGGGTGACGGACTAGCCGACTTGGCCTCAGTTTCCCCTCCT1190.10575333700655848No Hit
NNNNGNTGTGAGCACTACTGATAATCCCTGGCCTCAGTTTCCCCTCCTTC1190.10575333700655848No Hit
NNNNGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC1180.10486465350230169No Hit
NNTAGNTGATGTGAAAAGGACCCATGGCTCTATGTGTGAGAGAAAACTAC1180.10486465350230169No Hit
NNNNGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT1180.10486465350230169No Hit
NNTAGNTGTGAGCACTACTGATAATCCCTGGCCTCAGTTTCCCCTCCTTC1160.10308728649378811No Hit
NNNAGNCCTGACCTCATGATCTGCCTTTTGCTCATCCATTGTTGATCTAT1160.10308728649378811No Hit
NNNAGNTGTGAGCACTACTGATAATCCCTGGCCTCAGTTTCCCCTCCTTC1150.10219860298953132No Hit
NATAGACCAGCTTTGCATGAGTGTGGCATGGAGAAGGGTCTATGTGTAGA1140.10130991948527453No Hit
NNTAGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT1140.10130991948527453No Hit
NNNAGNCCTGACCTCATGATCTGCCTTCAGAACGCGGCAAATGGTCTATG1140.10130991948527453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)