FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66942
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA3221948.12972423889337No Hit
CATAGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA30074.491948253712168No Hit
CATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG20283.029488213677512No Hit
CATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG16312.4364375130710165No Hit
CATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT10261.5326700726001614No Hit
CATAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC7751.1577186220907651No Hit
CATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG7291.0890024200053778No Hit
CATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG6921.0337306922410443No Hit
CATAGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG5350.7991993068626573No Hit
CATAGATACGATCATAAGGGTGGGGCTGGGCTCAGTGGCTTATGCCTGTA3470.5183591766006393No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGACACTGGGGGCACAAAGTTCT2960.4421738221146664No Hit
CATAGACACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG2530.3779391114696304No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTTACGGTAGCAAAGCT2270.3390995189865854No Hit
CATAGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA2260.33760568850646827No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGACTTGGTCTCATAGAGGGGAG2240.33461802754623404No Hit
CATAGATGTGAGCACTACTGATAATCCCTGATGTGGCATGGAGAAGGGTC2140.3196797227450629No Hit
CATAGATGACCTGTTAATCTGCCCCCGAGACTTGGTCTCATAGAGGGGAG2040.30474141794389176No Hit
CATAGACTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC2000.29876609602342324No Hit
CATAGAGGGGAGACACTGGCTATAAGAACTTGGTCTCATAGAGGGGAGAC2000.29876609602342324No Hit
CATAGAGGCTGGGTGACTTGGTGAATCATGTCGGGAAAGCTGTCTGTGTA2000.29876609602342324No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCCTTGGCCTCAGTTTCCCCTCCTTC1910.2853216217023692No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGCAGAGACTTGGTCTCATAGAG1870.27934629978190073No Hit
CATAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1850.2763586388216665No Hit
CATAGACACCTTGTATATGCCAGGACTGAGGGGAAAGCTGTCTGTGTAGG1780.26590182546084673No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG1750.26142033402049536No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT1690.2524573511397927No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGAGACTTGGTCTCATAGAGGGG1670.24946969017955842No Hit
CATAGAGGGGAGACACTGGGGGCACAAAGTTCTATGTGTAGAACCATGTC1600.23901287681873862No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA1550.23154372441815302No Hit
CATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1530.22855606345791882No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCGGAGCCTTCAGGTCA1490.22258074153745033No Hit
CATAGACTTACTGCCCTCACTCCGTCTGCATAGCCACATCTATGTGTAGA1470.21959308057721608No Hit
CATAGATGATGTGAAAAGGACCCATGGCGTCAGTGTGCAAAGCTGCTATG1460.21809925009709896No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA1410.2106300976965134No Hit
CATAGACCTGACCTCATGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGG1410.2106300976965134No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA1400.20913626721639628No Hit
CATAGACCTGACCTCATGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGG1310.19569179289534222No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGGGAGACACTGGGGGCACAAAG1260.18822264049475665No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGGTCCGCGCAGAGCCTTCAGGTCA1250.18672881001463953No Hit
CATAGACCAGCTTTGCATGAGTGTGGCATGGAGAAGGGTCTATGTGTAGA1230.1837411490544053No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGAGGGGAGACACTGGGGGCACA1230.1837411490544053No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCTGCGCAGAGCCTTCAGGTCA1200.17925965761405396No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA1190.17776582713393685No Hit
CATAGACACACCCAACTCTGCTCAGTGTACCTCCAAGCAGATCTGTGTTC1180.1762719966538197No Hit
CATAGAGGTGAGCGGCTGTCTCCAGAAGTCCGCGCAGAGCCTTCAGGTCA1160.1732843356935855No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCCAGAGACTTGGCCTCAGTTTCCCC1160.1732843356935855No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGCCA1140.17029667473335125No Hit
CATAGACCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG1120.16730901377311702No Hit
CATAGAGTCTCAATTATTCATCTCAACATGCTAGAACTTGGCCTCAGTTT1100.1643213528128828No Hit
CATAGAGTTATTCAGAGAGAGGGGAAATGAGAGAAAACTACCCCAGGCTC1080.16133369185264856No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTTCTATGTGTAGAACCATGGCCTC1070.15983986137253145No Hit
CATAGATGATGTGAAAAGGACCCATGGCGTCAGTGTACCAGTGTAGCAGA1050.1568522004122972No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGCCCGCGCAGAGCCTTCAGGTCA1040.15535836993218008No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTTAGAGACTTGGCCTCAGTTTCCC1030.153864539452063No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGTCCGCGCAGAGCCTTCAGGTCAGA1000.14938304801171162No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTTCTATGTGTATGGCCTCAGTTTC990.14788921753159454No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGGGCCTTCAGGTCA940.14042006513100894No Hit
CATAGACCTGACCTCATGATCTGCCTACCTCAGCCTCCCAAAGTGCTGGG930.13892623465089182No Hit
CATAGATGGTGGGTTCTGGAGAGAGGGCGAAAGTGATCAGAAGCTCTATG900.13444474321054048No Hit
CATAGACCTCCTTTCCTCCACGGCAAAGCTGCTATGGGATCTTCTATGTG860.12846942129007202No Hit
CATAGAGGGGAGACACTGGCTATAAGAACAGAGACTTGGTCTCATAGAGG830.12398792984972065No Hit
CATAGACTTTTCCTCCAATATAGCTGCACTAGACTTGGCCTCAGTTTCCC820.12249409936960352No Hit
CATAGACCTGACCTCATGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGG790.11801260792925218No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGTGCAGAGCCTTCAGGTCA780.11651877744913507No Hit
CATAGACACCATTGGAGACCTGAGCACCTTCCACTCCAAAGCACAGTATC780.11651877744913507No Hit
CGTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA770.11502494696901797No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCGAGACTTGGCCTCAGTTTCCCCTC750.11203728600878372No Hit
CATAGACCTGACCTCATGATCTGCCTACCTCAGCCTCCCAGATTGCTGGG750.11203728600878372No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT750.11203728600878372No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTTCCCTTGCCCTGTGGCCCTGGCT740.1105434555286666No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCAAGCAGATCTGTGTTCTATGTGTA730.10904962504854948No Hit
CATAGATACGATCATAAGGGTGGGGCATGGTGGCTCACACCTGTAATCCC730.10904962504854948No Hit
CATAGACTTACTGCCCTCACTCAGTCTGCATAGCCACATCTATGTGTAGA720.10755579456843238No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCAGAGACTTGGCCTCAGTTTCCCCT700.10456813360819814No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGGGGAGACACTGGGGGCACAAA700.10456813360819814No Hit
CATAGAAAGGCAATGTTCAAGTGGTTTTACTTGGCCTCAGTTTCCCCTCC700.10456813360819814No Hit
CATAGATGACCTGTTAATCTGCCCCCAGATCACAGAGTGGCCTTGGGCAA680.10158047264796392No Hit
CATAGAGGTGAGTGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA670.1000866421678468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)