FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118409
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNTAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG14421.217812835173002No Hit
NNNAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG14161.1958550448023377No Hit
NNNNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG12521.0573520593873775No Hit
NNTAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG11040.9323615603543649No Hit
NNTNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG10930.9230717259667762No Hit
NATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG10920.9222271955679046No Hit
NNNNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG10260.8664881892423717No Hit
NNNAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG9990.8436858684728358No Hit
NATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG8730.7372750382150005No Hit
NNTNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG8480.7161617782432078No Hit
NNTAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA8470.7153172478443361No Hit
NNTAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT8380.7077164742544908No Hit
NNNAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT8100.684069623086083No Hit
NNNAGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT8040.6790024406928528No Hit
NNTAGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT7930.669712606305264No Hit
NNNAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA7910.6680235455075205No Hit
NATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA7910.6680235455075205No Hit
NNNNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA7580.6401540423447541No Hit
NNNNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT7310.6173517215752181No Hit
NNNNGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT6920.5844150360192215No Hit
NATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT6360.5371213336824059No Hit
NNNAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG6150.5193861953061No Hit
NNTNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT6140.5185416649072284No Hit
NATAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT5960.5033401177275376No Hit
NNTNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA5860.4948948137388205No Hit
NNTAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG5820.4915166921433337No Hit
NNTAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT5730.48391591855348837No Hit
NNTNGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT5650.4771596753625147No Hit
NNNAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT5360.4526682937952352No Hit
NNNNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT5090.4298659730256991No Hit
NNNNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG5040.4256433210313405No Hit
NATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT4980.4205761386381103No Hit
NNTAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT4870.4112863042505215No Hit
NATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG4570.3859503922843703No Hit
NNTAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG4530.38257227068888344No Hit
NNNNGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT4440.3749714970990381No Hit
NNNAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG4410.3724379059024229No Hit
NNNNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG4410.3724379059024229No Hit
NNNAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT4390.37074884510467954No Hit
NNTNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG4290.36230354111596247No Hit
NNTNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT4190.3538582371272454No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC4020.3395012203464264No Hit
NNNNGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3990.33696762914981127No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG3980.33612309875093954No Hit
NATAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT3880.3276777947622225No Hit
NNTAGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT3840.32429967316673564No Hit
NATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3840.32429967316673564No Hit
NNNAGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT3650.30825359558817317No Hit
NNTNGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT3600.30403094359381466No Hit
NNTNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3560.3006528219983278No Hit
NNNAGNCCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3450.2913629876107391No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3390.2862958052175088No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG3360.2837622140208937No Hit
NNNAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3360.2837622140208937No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3310.2795395620265352No Hit
NNNNGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT3080.2601153628524859No Hit
NNTAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3060.2584263020547425No Hit
NNNAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG3040.25673724125699904No Hit
NATAGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT3020.25504818045925565No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2910.24575834607166686No Hit
NATAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC2910.24575834607166686No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2870.24238022447618002No Hit
NNNNGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2810.2373130420829498No Hit
NNTNGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT2740.2314013292908478No Hit
NNNAGNTACGATCATNAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2600.2195779037066439No Hit
NATAGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2570.2170443125100288No Hit
NNNNGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT2500.21113259971792686No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC2480.20944353892018344No Hit
NNTAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2410.20353182612808146No Hit
NNTAGNCCTGACCTCATGATCTGCCTTTTGCTCATCCATTGTTGATCTAT2360.19930917413372293No Hit
NNNNGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA2310.1950865221393644No Hit
NNNAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA2280.19255293094274928No Hit
NNNAGNCACCATTGGNGACCTGAGCATCCCTCATTACTCTGGTTCTATGT2200.18579668775177563No Hit
NNNNGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT2130.17988497495967368No Hit
NNNAGNCCTGACCTCATGATCTGCCTTTTGCTCATCCATTGTTGATCTAT2010.1697506101732132No Hit
NNNAGNGTTTGCCCTNCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT2000.16890607977434147No Hit
NNNAGNGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA2000.16890607977434147No Hit
NNNAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1960.16552795817885466No Hit
NNNAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1950.16468342777998293No Hit
NNNAGNCCTGACCTCATGATCTGCCTTTCTGGGTAGCTGCGACCTCTATG1950.16468342777998293No Hit
NNTAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1930.1629943669822395No Hit
NATAGACCTGACCTCATGATCTGCCTTTTGCTCATCCATTGTTGATCTAT1910.16130530618449612No Hit
NNTAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1910.16130530618449612No Hit
NNNNGNCCTGACCTCATGATCTGCCTTTTGCTCATCCATTGTTGATCTAT1880.15877171498788098No Hit
NNTAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1880.15877171498788098No Hit
NNTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA1880.15877171498788098No Hit
NNNAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1860.1570826541901376No Hit
NATAGACCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG1830.15454906299352245No Hit
NNNNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1820.15370453259465075No Hit
NNTNGNCCTGACCTCATGATCTGCCTTTTGCTCATCCATTGTTGATCTAT1730.14610375900480538No Hit
NNNAGNTGGTGGGTTNTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1690.14272563740931854No Hit
NNTAGNTTAGGGGTGACGGACTAGCCATGCTGAAGTCCCATGATCTATGT1660.14019204621270343No Hit
NNNAGNTTAGGGGTGACGGACTAGCCATGCTGAAGTCCCATGATCTATGT1660.14019204621270343No Hit
NNNNGNCCTGACCTCATGATCTGCCTTTCTGGGTAGCTGCGACCTCTATG1610.1359693942183449No Hit
NNTNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1590.13428033342060147No Hit
NNNNGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1580.13343580302172978No Hit
NNNNGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1550.13090221182511463No Hit
NNTAGNCCTGACCTCATGATCTGCCTTTCTGGGTAGCTGCGACCTCTATG1540.13005768142624294No Hit
NATAGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1510.12752409022962782No Hit
NNTAGNTGACCTGTTAATCTGCCCCCGTAAGGTGGGAGGATTGCTTGAGT1500.1266795598307561No Hit
NNNAGNGGCTGGGTGACTTGGTGAATGAATGTTGGAGAGGCACATCTATG1490.1258350294318844No Hit
NATAGACCTGACCTCATGATCTGCCTTTCTGGGTAGCTGCGACCTCTATG1480.1249904990330127No Hit
NNNAGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1460.12330143823526929No Hit
NATAGATACGATCATNAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1440.12161237743752587No Hit
NNNAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1420.11992331663978244No Hit
NATAGAGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA1420.11992331663978244No Hit
NNTAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1410.11907878624091074No Hit
NNNAGNTGACCTGTTAATCTGCCCCCGTAAGGTGGGAGGATTGCTTGAGT1370.1157006646454239No Hit
NNNNGNTTAGGGGTGACGGACTAGCCATGCTGAAGTCCCATGATCTATGT1360.11485613424655221No Hit
NNTAGNGGCTGGGTGACTTGGTGAATGAATGTTGGAGAGGCACATCTATG1350.11401160384768048No Hit
NNTAGNTGTGAGCACTACTGATAATCCCTGATGTGGCATGGAGAAGGGTC1350.11401160384768048No Hit
NNTAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1320.11147801265106538No Hit
NNTAGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1310.11063348225219367No Hit
NNTAGNTGTGAGCACTACTGATAATCCCTACACTGGCCTCAGTTTCCCCT1300.10978895185332195No Hit
NNNAGNGTTATTCAGAGAGAGGGGAAATGAGAGAAAACTACCCCAGGCTC1300.10978895185332195No Hit
NATAGACACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1300.10978895185332195No Hit
NNNNGNTGACCTGTTAATCTGCCCCCGTAAGGTGGGAGGATTGCTTGAGT1270.10725536065670685No Hit
NNNAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1260.10641083025783513No Hit
NNNNGNTGTGAGCACTACTGATAATCCCTGATGTGGCATGGAGAAGGGTC1260.10641083025783513No Hit
NNTAGNGTTATTCAGAGAGAGGGGAAATGAGAGAAAACTACCCCAGGCTC1250.10556629985896343No Hit
NNTNGNCCTGACCTCATGATCTGCCTTTCTGGGTAGCTGCGACCTCTATG1230.10387723906122No Hit
NATAGATTAGGGGTGACGGACTAGCCATGCTGAAGTCCCATGATCTATGT1220.1030327086623483No Hit
NNTAGNCTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC1210.10218817826347659No Hit
NNTAGNTCCACTGTGCTATCTCTGACACCGTAGCCATGCTGAAGTCCCAT1210.10218817826347659No Hit
NNNAGNTGTTTGAAACTCCTCTACCTAAGATTTATCCAGCATGCCCCTCT1200.1013436478646049No Hit
NNNNGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1190.10049911746573317No Hit
NNNAGNTGTGAGCACTACTGATAATCCCTGATGTGGCATGGAGAAGGGTC1190.10049911746573317No Hit
NNTAGNCTTACTGCCCTCACTCCGTCAGTGTATGAATGTTGGAGAGGCAC1190.10049911746573317No Hit
NATAGATGACCTGTTAATCTGCCCCCGTAAGGTGGGAGGATTGCTTGAGT1190.10049911746573317No Hit
NNTAGNTGTTTGAAACTCCTCTACCTAAGATTTATCCAGCATGCCCCTCT1190.10049911746573317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)