FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40002
Sequences flagged as poor quality0
Sequence length150
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG436310.906954652267387No Hit
CATAGAGCTTGGGTGCGAACATCAAGGAAGTTTCTGTGAGATCTATGAGA402210.054497275136242No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGTAATAACCAGGCCCCTCTATGAG20175.04224788760562No Hit
CATAGAGAAGACTCCCTCAACCCACCCACAGCCAGGAAAGAATCTATGAG17004.249787510624469No Hit
CATAGACGGATTATTCTCTCCATGGTCTACCAGTTATTAGTTGAATGAGC16914.227288635568222No Hit
CATAGAAATGATGCTGAAGGGCCTGCCCTCTCTGCTGTTCCTTCTATGAG11732.9323533823308834No Hit
CATAGACTCATTGCACTTTCCACAACTGTGTGCTTGAACCAGATTCTATG11092.7723613819309034No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACCAGTGAAGCAGAGCTTCCCA9742.4348782560871958No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGTTCTGGGCCCCAGTGTGAA9622.4048797560121993No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAGGAACCATGGGAGGTCTAT6301.574921253937303No Hit
CATAGAAATGATGCTGAAGGGCCTGTTTCCCCACTCAAATACCATCTATG6201.5499225038748061No Hit
CATAGACTCATTGCACTTTCCACAACTGTCATTGTCGCTGTCCCATCTAT6091.5224238788060598No Hit
CATAGACTCATTGCACTTTCCACAACTGTCCCAACTGTCTCTGAAAAATT5781.4449277536123195No Hit
CATAGATCATGGGACTTCAGCATGGCTCAGGTATGTCTTTATTAGCTCTA5101.2749362531873407No Hit
CATAGATCAAGGAAAACACCAGCCCATGCAAACTGTTCTAGGCAGAAGAA4741.184940752962352No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAAGGAAGTTTCTGTGAGATC4621.1549422528873556No Hit
CATAGACGTGACTAAAAGCATGTTGGTGGAAGGGTAAGAGAGGGTCTATG4441.1099445027748611No Hit
CATAGACACAACAGAGAGAACGGGCTACACTGACGCTATACCACACTACA4261.0649467526623668No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTCTTCCTTTTCTGCCTTCATCTA4161.03994800259987No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGAAGTGATGGGCTAGATCTATGA4161.03994800259987No Hit
CATAGACCAACATTTCTTCTCCCAGGGTATAAAGCTGGACCCTCTCTGGG3960.9899505024748764No Hit
CATAGATGTTTTATCAAAGGCATGCTCATTTATTTTAAAGGACTGGAAGC3710.9274536273186341No Hit
CATAGAAGCTGAGAAGACTTCCTAATGCAGAAGTAGGAAGTATTACCCCT3550.887455627218639No Hit
CATAGACCAGCAGACACTCCCATCATTTATTTTAAAGGACTGGAAGCCTC3130.7824608769561523No Hit
CATAGATCACGTAGAAACTGGAGAGGCTAATCTGAAATCTTCGTGTTCTA2830.7074646267686615No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGACGATCATTGTTCACT2670.6674666266686666No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTGCCTCTAAGTAAACTGAAT2620.6549672516374182No Hit
CATAGATCAAGGAAAACACCAGCCCACGCTGTCCTTCTCTGAATCTATGA2560.63996800159992No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGGGGTAAGGCGTACTGGTCTATGA2550.6374681265936704No Hit
CATAGATGTGTAGCCTGGGTCTGACATGCCAGGAATCAATGTTCTATGAG2210.552472376381181No Hit
CATAGAGAAGACTCCCTCAACCCACGCTGTCCTTCTCTGAATCTATGAGA2140.5349732513374331No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGCCCCAGTGTGAATACTTCT1910.47747612619369034No Hit
CATAGAGTCTGAATGGTGGGAGAGTGAAAGAAATCTACAAAGGATTCTGG1800.4499775011249437No Hit
CATAGACGTGACTAAAAGCATGTTGGTACACTGGCTCAGGTATGTCTTTA1750.4374781260936953No Hit
CATAGATGTTTTATCAAAGGCATGCTCATACACTGACGCTATACCACACT1610.4024798760061997No Hit
CATAGAGCTGTACTGCATCTTTTCTCAAACTGCTGGGGTTGCTCTATGAG1600.39998000099995No Hit
CATAGAAGCCATGAACACAGGATTGAAAAAAGATTGATAGACTACAGGAA1560.38998050097495124No Hit
CATAGACCAACATTTCTTCTCCCAGGGTGGAAGGGTAAGAGAGGGTCTAT1550.38748062596870153No Hit
CATAGACCAGCAGACACTCCCATCATCAAGGAAGTTTCTGTGAGATCTAT1490.37248137593120345No Hit
CATAGACGGATTATTCTCTCCATGGTCTCAAACTGCTGGGGTTGCTCTAT1400.34998250087495625No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTCTGAAAAATTTCTATGAGA1330.3324833758312084No Hit
CATAGATGGCCAAGATACGAGCACATAATAATTCTAACTCAGTCAAATTT1120.279986000699965No Hit
CATAGACCCAGATGCTGTCCCACTAAATCTACAAAGGATTCTGGGCCATC1080.26998650067496627No Hit
CATAGAGAAGACTCCCTCAACCCACCACACTGACGATCATTGTTCACTGC1070.26748662566871656No Hit
CATAGACCTCCGACAGTTTTTATGCACCTTCATTGTTGCTCTATGAGACC1070.26748662566871656No Hit
CATAGATCAAGGAAAACACCAGCCCACCTTGCTCCGGGACATCCAGAAGA1030.25748712564371784No Hit
CATAGACACAACAGAGAGAACGGGCTACACTGACGATCATTGTTCACTGC1010.2524873756312184No Hit
CATAGATCATGGGACTTCAGCATGGCCACTGACGCTATACCACACTACAC1000.24998750062496874No Hit
CATAGAAAAAACAGTTGGCCAGGCATCTATGTGCTGTCATTGTCGCTGTC1000.24998750062496874No Hit
CATAGAGTGCCTGTTTCCTCATTCGTACATAGAGTGTCTGTTTCCCCACT990.2474876256187191No Hit
CATAGAAGCCATGAACACAGGATTGAGGAGCTGAGTCATGGGGTCTATGA980.24498775061246938No Hit
CATAGACGTGACTAAAAGCATGTTGGTGTTTGCAGATTTGGACCTGCGAG970.24248787560621968No Hit
CATAGAGTGCCTGTTTCCTCATTCGTGTTCAGGCTAATCTGAAATCTTCG950.2374881255937203No Hit
CATAGACCCAAGACCCCAGCCTTTACACACTGACGCTATACCACACTACA950.2374881255937203No Hit
CATAGACTTGAAAGTGATAACTGAGAGGTAAACAAATGATTTTTCCACTA940.23498825058747064No Hit
CATAGAGAAGACTCCCTCAACCCACCACGCTGTCCTTCTCTGAATCTATG930.23248837558122096No Hit
CATAGATGTGTAGCCTGGGTCTGACAAGGGGTAAGGCGTACTGGTCTATG920.22998850057497125No Hit
CATAGAACAGATGTTCTCCTTGTTTTCTATAACACACTGACGATCATTGT880.2199890005499725No Hit
CATAGATTGTGTGTCTCTTCTATATTAACAGTCACACCCACAGCCAGGAA870.21748912554372282No Hit
CATAGAAATGATGCTGAAGGGCCTGCACACTGACGACATGGTTCTACACA860.21498925053747311No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGACGCTATACCACACTA850.21248937553122343No Hit
CATAGACCTCCGACAGTTTTTATGCACTGTCCCAACTGTCTCTGAAAAAT840.20998950052497373No Hit
CATAGAGTCTGAATGGTGGGAGAGTGCTGAAGTGATGGGCTAGATCTATG800.199990000499975No Hit
CATAGACGGATTATTCTCTCCATGGTCTGAGGAGAATTCAAGCTGCTCTA780.19499025048747562No Hit
CATAGACTCATTGCACTTTCCACAACTGTCCACTGTGTGCTTGAACCAGA760.18999050047497626No Hit
CATAGAGTCCAACTATTCCAGCACCACGACATGGTTCTACACATAGAGAT750.18749062546872655No Hit
CATAGAGTGCCTGTTTCCTCATTCGTCTGTTTCCCCACTCAAATACCATC710.17749112544372783No Hit
CATAGACATTTAATCCTCACAATGACCCATTGCTTCTTGTGGTAACTGGT700.17499125043747812No Hit
CATAGATTGTGTGTCTCTTCTATATTAACAGTCACACTGCCCTCTCTGCT670.16749162541872906No Hit
CATAGATCATGGGACTTCAGCATGGCACACTGACGCTATACCACACTACA670.16749162541872906No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACTGCTGGGGTTGCTCTATGAG660.16499175041247938No Hit
CATAGACCTCCGACAGTTTTTATGCACCCACAGCCAGGAAAGAATCTATG630.1574921253937303No Hit
CATAGAAATGATGCTGAAGGGCCTGCAGAAGTAGGAAGTATTACCCCTCT620.15499225038748063No Hit
CATAGACGTGACTAAAAGCATGTTGGTCTACCTGAGGAGCTGAGTCATGG610.15249237538123092No Hit
CATAGAAAATTTGTGATGGTAAATCATGGGACATGCCAGGAATCAATGTT610.15249237538123092No Hit
CATAGACCGCTTCCCAATTTTTGTAACAACACTGACGACATGGTCTACCA580.14499275036248185No Hit
CATAGATGTTTTATCAAAGGCATGCTCATACACTGCCCTCTCTGCTGTTC570.1424928753562322No Hit
CATAGAAATGATGCTGAAGGGCCTGCGATGCTGAAGTGATGGGCTAGATC540.13499325033748313No Hit
CATAGATCACGTAGAAACTGGAGAGGCCTCATAGAAGGAAGTAGGACATG540.13499325033748313No Hit
CATAGAAATGATGCTGAAGGGCCTGCCACTGACGACATGGTTCTACACAT530.13249337533123343No Hit
CATAGAAATGATGCTGAAGGGCCTGCACTGACGACATGGTTCTACACATA530.13249337533123343No Hit
CATAGACATTTAATCCTCACAATGACCCATGCTTCTCAAACTGCTGGGGT520.12999350032498375No Hit
CATAGATCAAGGAAAACACCAGCCCAACCAACATGGTGAAACACTGTCTC510.12749362531873407No Hit
CATAGACTCATTGCACTTTCCACAACTGTTACGGTAGCAGCATAATTTCC500.12499375031248437No Hit
CATAGATTGTGTGTCTCTTCTATATTAACAGTCTCTGCTACCGTAACGCT500.12499375031248437No Hit
CATAGAAATGATGCTGAAGTGATGGGCTAGATCTATGAGACCAAGTCTCT500.12499375031248437No Hit
CATAGAGTCTGAATGGTGGGAGAGTGATAGGCTTAGAGGGCTTGCTCTAT500.12499375031248437No Hit
CATAGAGTGCCTGTTTCCTCATTCGTGTTTCCTCTGTTTCCCCACTCAAA490.12249387530623469No Hit
CATAGAGAAGACTCCCTCAACCCACCATTGATTCCTGTCATTGTCGCTGT490.12249387530623469No Hit
CATAGAAATGATGCTGAAGGGCCTGCAGTCTTTGGAGGAATCTATGAGAC480.119994000299985No Hit
CATAGACACAACAGAGAGAACGGGCTACACTGACGACATGGTTCTAACAT480.119994000299985No Hit
CATAGACTCATTGCACTTTCCACAACTGTAGGACATGCCAGGAATCAATG470.11749412529373532No Hit
CATAGAAGCTGAGAAGACTTCCTAATGCACACTGACGACATGGTTCTACA470.11749412529373532No Hit
CATAGACACAACAGAGAGAACGGGCTGCCCTCTCTGCTGTTCCTTCTATG440.10999450027498625No Hit
CATAGACCCAAGACCCCAGCCTTTACAGCTTGCCTGCTGGGTTTATTTCT440.10999450027498625No Hit
CATAGATGTGTAGCCTGGGTCTGTCATTGTCGCTGTCCCATCTATGAGAC440.10999450027498625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)