FastQCFastQC Report
Wed 8 May 2024
EGAF00005807527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506393
Total Bases75.9 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG7531514.872835919927804No Hit
CATAGAGAATTTGCACACCACGGGACATGGATGAAGCTGGATCTATGAGA505159.97545384711084No Hit
CATAGATGTCTCCCAGGCTAGAGTGAGCCCTTGTCCCTTTCTATGAGACC327796.473035764712387No Hit
CATAGATCATGGGACTTCAGCATGGTGGGTGCCTGATTGTTCTATGAGAC220694.358077619556353No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGAGAGGCTGTTTGAGATCT170863.3740592780705896No Hit
CATAGAGAATTTGCACACCACGGGACCATGCTCACACCTTGATCTATGAG156583.0920648587164514No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAATCAGGGTTGGTTCTATGAGACC144962.862598811594947No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGTGAGAAATGATGATGGCT130072.568558412142348No Hit
CATAGAGGGTGTTTTTCGGTGTTACAACCAATGCTCTTTCATCTATGAGA124802.4644890430949875No Hit
CATAGAACAAGGCCAGAATTACCCTGGGAGAGGCTGTTTGAGATCTATGA105482.0829671816158597No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGCAACAGAACAACAACATCTATG104302.059665121753263No Hit
CATAGACCTTCCTGTTGGCCACACAGAATTAGCGGAGAGTGAGAACTCTA97051.916495686156799No Hit
CATAGAGGGTGTTTTTCGGTGTCACAACCAATGCTCTTTCATCTATGAGA86391.7059872470591024No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGGAACAGAACAACAACATCTATG78741.5549188081193857No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAGTGAGCCCTTGTCCCTTTCTATG60331.1913671792461586No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACCACGGGACATGGATGAAGC57721.1398261824314317No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGCAACAGAACAACAACATC56791.121460999658368No Hit
CATAGATTCTCCCTTGTGCTTTTGGTACACTGACGACATGGTGGGTGCCT52121.0292401356258873No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCCAGGCCTGAGATTGCTGCTAT47570.9393889725963827No Hit
CATAGAGGGCCAGAAGTTTGTTTACCTACATAGAGGCCTGAGATTGCTGC37290.7363845866747762No Hit
CATAGACAGCTCACAGACTGCATACTACACTGGAGACAGCCGCTCACCTC33150.654629902072106No Hit
CATAGAGCAAAAGAGAAACATTAAGCCATAGTACACTGACGACATGGTGG30830.6088156826812378No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACCACGGGACCATGCTCACAC30510.6024964800066351No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGCTGACGACATGGTGGGTGCC29390.5803792706455263No Hit
CATAGATGTCTCCCAGGCTAGAGTGCGCCCTTGTCCCTTTCTATGAGACC27770.5483883071053509No Hit
CATAGAGAAGAGAAGGCGACAATGGCTGCTTTTCTCTTCTTGGCATCTAT27060.5343675761710767No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAACCCAAAGAGTGAGCCCTTGTCC26030.5140276425621997No Hit
CATAGAGGGCCAGAAGTTTGTTTACCTACACTGACGACATGGTGGGTGCC25940.5122503668099677No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAGAGTGAGCCCTTGTCCCTTTCTA25710.5077084398875972No Hit
CATAGACTTGATGCCCAGGAAACCTCACACTGACGACATGGTGGGTGCCT24790.48954073219811495No Hit
CATAGACAGGAGGAGGAAGAAGAGGAATTCTTGCCAGGGATCTATGAGAC24060.47512505109667785No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTGCTACTGGGCAACAGAACAA23820.47038564909072594No Hit
CATAGATTCTCCCTTGTGCTTTTGGTGTTCTACACATAGATTCTCCCTTG22490.4441214629744092No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACATGGTGGGT20630.407391097428282No Hit
CATAGACCCTTAGAGTTTCAGCCTCCACACTGACGACATGGTTCTACACA18160.3586147517836937No Hit
CATAGAACCCAAAGAGTGAGCCCTTGTCCCTTTCTATGAGACCAAGTCTC17250.34064451917779276No Hit
CATAGAACAAGGCCAGAATTACCCTGGGTGAGAAATGATGATGGCTCTAT16630.3284010639957503No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCCCAAAGAGTGAGCCCTTGTCCCT16200.31990963540175316No Hit
CATAGAGAAGAGAAGGCGACAATGGCCGCTAAGCCGAGAAGATCTCTATG15360.30332172838092153No Hit
CATAGAGAATTTGCACACCACGGGACATGGATGAAGCTGGACCTATGAGA13800.2725156153422342No Hit
CATAGAGAAGAGAAGGCGACAATGGGACATGGATGAAGCTGGATCTATGA13520.266986313001957No Hit
CATAGACCACACCCAAACACCACGTACACTGACGACATGGTTCTACACAT13440.26540651233330637No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAAGAGTGAGCCCTTGTCCCTTTCT12790.2525706319005199No Hit
CATAGATTCTCCCTTGTGCTTTTGGTACACTGACGACATGGTTCTACACA12280.24249940263787215No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCCAGGCTAGAGTGAGCCCTTGT12190.2407221268856402No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCACTGACGACATGGTTCTACACAT11610.2292685720379231No Hit
CATAGAATGCAGGAGAATATACACAGGTTGTCATAGACTCTTCACTATGT11490.22689887103494716No Hit
CATAGAATCAAAATAGTACCCTTGCCTCTATAGAAAGGGACATGGATGAA10680.2109033892648595No Hit
CATAGAACCCAAAGAGTGAGCAGCAGTTCTACACATAGAACCCAAAGAGT9350.18463920314854274No Hit
CATAGATGTCTCCCAGGCTAGAGTGAGCCCTTGTCCCTTTCCATGAGACC9020.17812252539035886No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGCACTGGGACATGGATGAAGC8870.17516039913663894No Hit
CATAGAGCTTCTTTCTGTTCATATTGAGGAATTCTTGCCAGGGATCTATG8550.1688411964620364No Hit
CATAGAACCCAAAGAGTGAGCAGCAGTAGAACCCAAAGAGTGAGCCCTTG8490.16765634596054843No Hit
CATAGAACCCAAAGAGTGAGCAGCAGGAACCCAAAGAGTGAGCCCTTGTC8320.16429926953966584No Hit
CATAGATCTGTGGCTCCCGATAAATGTGCTCATACAGAATTAGATCTAGC7900.15600531602925No Hit
CATAGAACCCAAAGAGTGGGCAACAGAACAACAACATCTATGAGACCAAG7870.155412890778506No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGTGGGCAACAGAACAACAACA7870.155412890778506No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCACATAGAACCCAAAGAGTGAGCC7850.15501794061134339No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCATAGAACCCAAAGAGTGAGCCCT7730.1526482396083674No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGACATGGATGAAGCTGGAT7580.1496861133546475No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGGAATTCTTGCCAGGGATCTATG7470.14751388743525284No Hit
CATAGAGAATTAGCGGAGAGTGAGAACTCTATGAGACCAAGTCTCTGCTA7440.14692146218450886No Hit
CATAGAGAATTTGCACACCACGGGACTTCAGTTGACTATAAGAAACACCT6720.13270325616665318No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAACAACAACATCTATGAGACCAAG6230.12302697707116804No Hit
CATAGATCATGGGACTTCAGCATGGCGGGTGCCTGATTGTTCTATGAGAC6130.12105222623535476No Hit
CATAGATCATGGGACTTCAGCATGGTGGGTGCCTGATTGTTTTATGAGAC6080.12006485081744811No Hit
CATAGACAGGAGGAGGAAGAAGAGGAGGGACATGGATGAAGCTGGATCTA5820.11493049864433355No Hit
CATAGATTAGTTCTTATTCTTGGTATGGCACTGGGGACATGGATGAAGCT5760.11374564814284557No Hit
CATAGAACCCAAAGAGTGAGCAGCAGACCCAAAGAGTGAGCCCTTGTCCC5730.11315322289210158No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACATGGGCAAC5650.11157342222345096No Hit
CATAGAGAAGAGAAGGCGACAATGGCCAGAATTAGCGGAGAGTGAGAACT5350.10564916971601108No Hit
CATAGACCCTTAGAGTTTCAGCCTCAGACATGTTTCCTAGTTTCAGTTCT5280.10426684413094177No Hit
CATAGATCTGTGGCTCCCGATAAATGTCGTCAGTGTCTGGGTGAGAAATG5110.10090976771005918No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)