FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150335
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA4782831.814281438121526No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA102536.820101772707619No Hit
CATAGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA40132.6693717364552496No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGAATTACAGGTGCCCGCCAC32032.130575049057106No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG21731.4454385206372435No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT19041.2665048059334154No Hit
CATAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT17941.1933348854225563No Hit
CATAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA17681.1760401769381714No Hit
CATAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA16181.0762630126051818No Hit
CATAGAGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA15721.0456646822097315No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT13590.9039811088568862No Hit
CATAGATTGCATAGCCATGTGTAGCCATGCTGAAGTCCCATGATCTATGT11660.7756011574151063No Hit
CATAGAGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT7990.5314796953470582No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC7490.498220640569395No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG6950.46230086140951876No Hit
CATAGAGGTGAGCGGCTGTCTCCAAACTGGACAATGAGAGGATCTATGTG6290.4183989091030033No Hit
CATAGAGGTGAGCGGCTGTCTCCACTAAAAATACAAAAATTAGCCAGGCA5910.3931220274719792No Hit
CATAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA5780.38447467322978685No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACGACAGCCCGGCTACTG4970.33059500448997237No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCCCACCAC4910.32660391791665283No Hit
CATAGAGGTGAGCGGCTGTCTCCAGAAAGCCATGCTGAAGTCCCATGATC4900.32593873682109953No Hit
CATAGATGCATCAGCAACTCACAGATTGGAAGTACACAAAAAGGGCATCT4740.3152958392922473No Hit
CATAGACTGTCATTGCTTGCCCATTGCTTGTTTTTCTCAGGTTTGTCAAA4700.31263511491003426No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCCTGCTAT4680.3113047527189277No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTGCCAC4640.30864402833671467No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCCTGCCAC4490.2986663119034157No Hit
CATAGAATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG4400.29267968204343636No Hit
CATAGAGCAGAAAACCTTTGAATAACAGATAGAGCAGGAATTAGACTCTT4080.27139388698573186No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCCCGCCAC3940.2620813516479861No Hit
CATAGAAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA3930.2614161705524329No Hit
CATAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT3860.25675990288356004No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTGTCAAATTTGTTAAAAGAGG3750.24944291083247414No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCCACCAC3680.2447866431636013No Hit
CATAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG3660.24345628097249475No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTGCAGGCGCATGCTTG3620.2407955565902817No Hit
CATAGAGCAGAAAACCTTTGAATAACAGAATAGAGCAGGAATTAGACTCT3530.23480892673030232No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGTCCGCGCAGAGCCTTCAGGTCAGA3500.23281338344364252No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTGTCAGGTTTGTCAAAGATCA3430.2281571157747697No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGTACCACAGGCATGTGCCAC3320.22084012372368378No Hit
CATAGAAAGGCCTTCCTGTATCAGTCAGTGTGCCATGCTGAAGTCCCATG3090.20554095852595872No Hit
CATAGAAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT3080.20487577743040544No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGTGTGCCAC3080.20487577743040544No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG2940.1955632420926597No Hit
CATAGAGGTGAGCGGCTGTCTCCAGTGTCCAGATGATATGAACCCAAAGG2900.19290251771044667No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGTGCACCAC2850.18957661223268035No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCGGAGCCTTCAGGTCA2770.18425516346825424No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA2740.18225962018159444No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCCGCCAC2650.17627299032161506No Hit
CATAGAGCAGAAAACCTTTGAATAACAGATCATAGAGCAGGAATTAGACT2630.17494262813050854No Hit
CATAGACATGCTAACATTTCTGGGGATACTGGGGACTGTGACTCTGTTTT2610.173612265939402No Hit
CATAGACCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG2520.16762563607942263No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACGCACCAC2480.16496491169720956No Hit
CATAGAGGTGAGCGGCTGTCTCCATCACTTATACGCCTCCCTGTCTATGT2480.16496491169720956No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGCCTGCCAC2370.15764791964612365No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGCGCCAC2370.15764791964612365No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA2260.15033092759503774No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGCACCAC2240.14900056540393122No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTAATACAAAAAATTAG2230.14833538430837795No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGGTCCGCGCAGAGCCTTCAGGTCA2220.1476702032128247No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGTGCAGAGCCTTCAGGTCA2200.14633984102171818No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCACACCAC2180.14500947883061163No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTGCAGGTGTGTGCCAC2170.14434429773505839No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA2140.1423487544483986No Hit
CATAGAGGTGAGCGGCTGTCTCCACTAAAAATACAAAAAATTAGCCAGGC2100.13968803006618552No Hit
CATAGAGGTGAGCGGCTGTCTCCACATCCAGCTGCTTTTTGTATTTTTAA2060.13702730568397245No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGCCCGCCAC2040.13569694349286593No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGCCCACCAC2010.13370140020620613No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGCCA1990.1323710380150996No Hit
CATAGAGGTGAGCGGCTGTCTCCACTAAAAATACAAAAATTAGCCATGCT1980.13170585691954634No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCACC1930.12837995144178002No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA1920.12771477034622675No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTTAGGTCA1880.1250540459640137No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGAGATTATAGGTACTAATTTT1730.11507632953071474No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGGGCCTTCAGGTCA1700.11308078624405495No Hit
CATAGACCATCCAGAGGTAATTCTGCTACCCCTAAAGCCCTTGTGAAGTT1690.11241560514850168No Hit
CATAGACTGCCTCAGCCTCCTGAGTATCTGGGACTACAGGCATACACCAC1640.10908969967073537No Hit
CATAGAGGTGAGCGGCTGTCTCCAAATCTATGAGACCAACTCAGATGGGA1630.1084245185751821No Hit
CATAGACCAGACTTTTGAAAGAAGCACATGGAAGTACACAAAAAGGGCAT1620.10775933747962883No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCAACATGGTG1510.10044234542854291No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)