FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344433
Sequences flagged as poor quality0
Sequence length150
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG8627025.046961237744352No Hit
CATAGAGAATTTGCACACCACGGGACATGGATGAAGCTGGATCTATGAGA293748.52821884081955No Hit
CATAGATGTCTCCCAGGCTAGAGTGAGCCCTTGTCCCTTTCTATGAGACC190225.522699625181095No Hit
CATAGATCATGGGACTTCAGCATGGTGGGTGCCTGATTGTTCTATGAGAC126783.6808319760301718No Hit
CATAGAGAATTTGCACACCACGGGACCATGCTCACACCTTGATCTATGAG103122.9939059265517534No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAATCAGGGTTGGTTCTATGAGACC72182.09561801569536No Hit
CATAGAGGGTGTTTTTCGGTGTTACAACCAATGCTCTTTCATCTATGAGA72162.095037351241025No Hit
CATAGAGGGTGTTTTTCGGTGTCACAACCAATGCTCTTTCATCTATGAGA53241.5457287774400246No Hit
CATAGACCTTCCTGTTGGCCACACAGAATTAGCGGAGAGTGAGAACTCTA50291.460080770425598No Hit
CATAGAACAATGTAATAAGCATCGGAGGAACACTGACGACATGGTTCTAC47051.3660131288233126No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACATGGTTCTA45881.3320442582447094No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGCAACAGAACAACAACATCTATG44591.2945914009400956No Hit
CATAGATTCTCCCTTGTGCTTTTGGTACACTGACGACATGGTGGGTGCCT41751.2121370484245122No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAGTGAGCCCTTGTCCCTTTCTATG40961.1892008024782759No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGGAACAGAACAACAACATCTATG33310.9670966486951017No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACCACGGGACATGGATGAAGC32480.9429990738401953No Hit
CATAGATCTGTGGCTCCCGATAAATGTGCTCATACAGAATTAGATCTAGC32320.9383537582055146No Hit
CATAGACCACACCCAAACACCACGTACACTGACGACATGGTTCTACACAT25010.7261209001460371No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCCAGGCCTGAGATTGCTGCTAT24770.719152926694016No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACTTGGGCAAC20830.6047620291900022No Hit
CATAGATGTCTCCCAGGCTAGAGTGCGCCCTTGTCCCTTTCTATGAGACC20430.5931487401033002No Hit
CATAGAACAAGGCCAGAATTACCCTGGGAGAGGCTGTTTGAGATCTATGA19250.5588895372975295No Hit
CATAGAGAAGAGAAGGCGACAATGGCTGCTTTTCTCTTCTTGGCATCTAT19200.5574378761616918No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAGAGTGAGCCCTTGTCCCTTTCTA17500.5080813975432087No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACCACGGGACCATGCTCACAC16620.4825321615524645No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAACCCAAAGAGTGAGCCCTTGTCC16250.47178986914726523No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGAGAGGCTGTTTGAGATCT16210.470628540238595No Hit
CATAGACAGCTCACAGACTGCATACTACACTGACGACATGGTGGGTGCCT16160.4691768791027573No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACATGGGCAAC15250.4427566464305105No Hit
CATAGACTTGATGCCCAGGAAACCTCCTGCCCCTGCAGGCTGAAAGTGCC14440.4192397360299391No Hit
CATAGAGCAAAAGAGAAACATTAAGCCATAGTGTTACAACCAATGCTCTT14050.4079167791704047No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTCTATAGAGAGAGGTCTTGGC12840.3727865796831314No Hit
CATAGACAGCTGACCAGAAAATGTTTCAGACATGTTTCCTAGTTTCAGTT12420.3605926261420944No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCACTGACGACATGGTTCTACACAT12230.355076313825911No Hit
CATAGAGCAAAAGAGAAACATTAAGCCATAGTACACTGACGACATGGTGG11740.34085003469470115No Hit
CATAGAACCCAAAGAGTGAGCCCTTGTCCCTTTCTATGAGACCAAGTCTC11460.33272073233400984No Hit
CATAGACTTGATGCCCAGGAAACCTCACACTGACGACATGGTGGGTGCCT10610.3080424930247682No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCCCAAAGAGTGAGCCCTTGTCCCT10500.3048488385259252No Hit
CATAGACAGGAGGAGGAAGAAGAGGACCATGCTCACACCTTGATCTATGA10220.2967195361652339No Hit
CATAGACAGGAGGAGGAAGAAGAGGAATTCTTGCCAGGGATCTATGAGAC10190.29584853948373124No Hit
CATAGATTCTCCCTTGTGCTCATACAGAATTAGATCTAGCTCTATGAGAC10020.2909128916218829No Hit
CATAGAATGCAGGAGAATATACACAGGTTACACTGACGACATGGTGGGTG9330.2708799679473221No Hit
CATAGAACCCAAAGAGTGAGCAGCAGTGTCTGGGAGAGGCTGTTTGAGAT9010.2615893366779606No Hit
CATAGAGAATTTGCACACCACGGGACATGGATGAAGCTGGACCTATGAGA7940.23052378837103296No Hit
CATAGAGCTTCTTTCTGTTCATATTGAGGAATTCTTGCCAGGGATCTATG7490.21745883814849334No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCCAGGCTAGAGTGAGCCCTTGT7470.21687817369415824No Hit
CATAGAACCCAAAGAGTGAGCAGCAGTTCTACACATAGAACCCAAAGAGT6500.18871594765890606No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCATAGAACCCAAAGAGTGAGCCCT6340.18407063202422533No Hit
CATAGAACCCAAAGAGTGAGCAGCAGTAGAACCCAAAGAGTGAGCCCTTG5880.17071534957451812No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCCAGGCAGTCTCAAACGTTGTG5860.170134685120183No Hit
CATAGAACCCAAAGAGTGAGCAGCAGGAACCCAAAGAGTGAGCCCTTGTC5490.15939239271498376No Hit
CATAGAACCCAAAGAGTGAGCAGCAGGTTCTACACATAGAACCCAAAGAG5380.15619873821614072No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAAGAGTGAGCCCTTGTCCCTTTCT5320.15445674485313543No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCACATAGAACCCAAAGAGTGAGCC5280.15329541594446525No Hit
CATAGAATCAAAATAGTACCCTTGCCTCTATGAGACCAAGTCTCTGCTAC5230.1518437548086275No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGGAATTCTTGCCAGGGATCTATG5230.1518437548086275No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCCTTGTGCTCATACAGAATTAG5190.1506824258999573No Hit
CATAGAACCCAAAGAGTGGGCAACAGAACAACAACATCTATGAGACCAAG5100.14806943585544938No Hit
CATAGAGGGCCAGAAGTTTGTTTACCTACACTGACGACATGGTGGGTGCC5100.14806943585544938No Hit
CATAGAGAATTTGCACACCACGGGACTTCAGTTGACTATAAGAAACACCT5050.14661777471961165No Hit
CATAGATCATGGGACTTCAGCATGGCGGCGTTTGCAGATTTGGACCTGCG4770.1384884723589203No Hit
CATAGAGAAGAGAAGGCGACAATGGGACATGGATGAAGCTGGATCTATGA4750.13790780790458523No Hit
CATAGAACCCAAAGAGTGAGCAGCAGGCAGTCTCAAACGTTGTGTCTATG4690.13616581454157994No Hit
CATAGATGTCTCCCAGGCTAGAGTGAGCCCTTGTCCCTTTCCATGAGACC4680.13587548231441238No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAACAACAACATCTATGAGACCAAG4500.1306495022253965No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTCGCAGATTTGGACCTGCG4290.12455252545487802No Hit
CATAGAACCCAAAGAGTGAGCAGCAGACCCAAAGAGTGAGCCCTTGTCCC4240.12310086431904028No Hit
CATAGACAGGAGGAGGAAGAAGAGGAGGGACATGGATGAAGCTGGATCTA4190.12164920318320253No Hit
CATAGACCTTCCTGTTGGCCACACAGCAGTGTAGATGCAGCCATAAAAAA4030.11700388754852177No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCCGCG3900.11322956859534367No Hit
CATAGATTCTCCCTTGTGCTTTTGGTGTGCTCATACAGAATTAGATCTAG3820.11090691077800328No Hit
CATAGAACCCAAAGAGTGAGCAGCAGACATGGTTCTACACATAGAACCCA3710.10771325627916023No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)