FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78941
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNTAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG9911.2553679330132632No Hit
NNNAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG9871.2503008576025134No Hit
NNTAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA9811.2427002444863886No Hit
NNNAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA9341.1831621084100785No Hit
NNNNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG8961.1350248920079553No Hit
NNNNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA8901.1274242788918305No Hit
NATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA8351.0577519919940208No Hit
NATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG7440.9424760263994629No Hit
NNTNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG7350.9310751067252758No Hit
NNNAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG7350.9310751067252758No Hit
NNTAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG7030.8905385034392775No Hit
NNNNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG6610.8373342116264046No Hit
NNTNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA6440.8157991411307179No Hit
NATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG5800.734725934558721No Hit
NNNAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT5690.720791477179159No Hit
NNTAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT5580.7068570197995971No Hit
NNTNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG5140.6511191902813493No Hit
NNNNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT5100.6460521148705995No Hit
NATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT4740.6004484361738514No Hit
NNNAGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT4350.5510444509190409No Hit
NNTNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT4260.5396435312448538No Hit
NNNAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT4250.5383767623921663No Hit
NNTAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT4190.5307761492760416No Hit
NNTAGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT4010.5079743099276675No Hit
NNTAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3880.49150631484273066No Hit
NNNAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3760.47630508861048126No Hit
NNNNGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT3750.4750383197577938No Hit
NNNAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3730.4725047820524189No Hit
NNNNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3670.4649041689362942No Hit
NNTAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3560.4509697115567322No Hit
NNNNGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT3530.44716940499866986No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3490.4421023295879201No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3360.4256343345029832No Hit
NNNAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT3290.41676695253417106No Hit
NNNNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3280.4155001836814836No Hit
NATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3130.39649865089117187No Hit
NNTAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT3090.39143157548042207No Hit
NATAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT3080.3901648066277346No Hit
NNTNGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT3050.38636450006967227No Hit
NNTNGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT3030.38383096236429737No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC2970.37623034924817267No Hit
NNTNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG2970.37623034924817267No Hit
NATAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC2860.3622958918686107No Hit
NATAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT2780.3521617410471111No Hit
NATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG2750.3483614344890488No Hit
NNNAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2680.3394940525202366No Hit
NNTNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG2590.3280931328460496No Hit
NNNNGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA2570.3255595951406747No Hit
NNTAGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT2550.3230260574352998No Hit
NNNNGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2520.3192257508772374No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC2510.31795898202455No Hit
NNTAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA2500.3166922131718625No Hit
NNNAGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT2480.3141586754664876No Hit
NNNNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2470.3128919066138002No Hit
NATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2390.3027577557923006No Hit
NNTNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2340.2964239115288633No Hit
NNNAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA2320.2938903738234884No Hit
NNNAGNGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA2310.292623604970801No Hit
NNNAGNCCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2310.292623604970801No Hit
NATAGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT2260.2862897607073637No Hit
NNNAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA2240.2837562230019888No Hit
NNNNGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT2160.27362207218048923No Hit
NNTNGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT2100.26602145906436453No Hit
NNTAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2080.26348792135898963No Hit
NATAGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA2070.2622211525063022No Hit
NNTAGNGGCTGGGTGACTTGGTGAATCTCAGTACAACTGACAAGGGCACA2010.2546205393901775No Hit
NNTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA1970.24955346397942768No Hit
NNTNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1930.24448638856867788No Hit
NNNAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1930.24448638856867788No Hit
NNNAGNTACGATCATNAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1870.23688577545255318No Hit
NNNAGNGGCTGGGTGACTTGGTGAATCTCAGTACAACTGACAAGGGCACA1860.23561900659986573No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1850.23435223774717828No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1830.23181870004180338No Hit
NNNNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1800.228018393483741No Hit
NNNNGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1710.21661747380955398No Hit
NNTNGNGGCTGGGTGACTTGGTGAATCTCAGTACAACTGACAAGGGCACA1670.21155039839880416No Hit
NNNNGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1650.20901686069342926No Hit
NNNNGNGGCTGGGTGACTTGGTGAATCTCAGTACAACTGACAAGGGCACA1590.20141624757730459No Hit
NNNAGNCACCATTGGNGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1560.1976159410192422No Hit
NNTAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1500.19001532790311754No Hit
NNTAGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1500.19001532790311754No Hit
NATAGAGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA1480.1874817901977426No Hit
NATAGAGGCTGGGTGACTTGGTGAATCTCAGTACAACTGACAAGGGCACA1470.18621502134505516No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1430.18114794593430536No Hit
NATAGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1420.1798811770816179No Hit
NNNAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1390.17608087052355556No Hit
NNNAGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1370.17354733281818066No Hit
NNTAGNGGCTGGGTGACTTGGTGAATGTTGGAGAGGCACATCTATGTGTA1320.1672134885547434No Hit
NNTAGNAAGGCAATGTTCAAGTGGTTTTGAGGTAAAACTATTAGCGAGAC1290.16341318199668106No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1270.16087964429130616No Hit
NNNNGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1260.1596128754386187No Hit
NNNAGNAAGGCAATGTTCAAGTGGTTTTGAGGTAAAACTATTAGCGAGAC1240.1570793377332438No Hit
NNTAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1220.1545458000278689No Hit
NATAGACCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG1180.1494787246171191No Hit
NNNAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1150.14567841805905676No Hit
NNTNGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1110.14061134264830696No Hit
NNNNGNAAGGCAATGTTCAAGTGGTTTTGAGGTAAAACTATTAGCGAGAC1090.13807780494293206No Hit
NNNAGNCCTGACCTCNTGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1080.13681103609024461No Hit
NATAGAAAGGCAATGTTCAAGTGGTTTTGAGGTAAAACTATTAGCGAGAC1080.13681103609024461No Hit
NNNNGNGGCTGGGTGACTTGGTGAATGTTGGAGAGGCACATCTATGTGTA1070.13554426723755716No Hit
NNNAGNGTTTGCCCTNCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1060.13427749838486971No Hit
NNNNGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1040.13174396067949481No Hit
NNNAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1040.13174396067949481No Hit
NNNNGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1030.13047719182680736No Hit
NNNNGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1020.12921042297411992No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGCATGGAGAAGGGTCTATGTGTAGA1020.12921042297411992No Hit
NNNAGNGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT1020.12921042297411992No Hit
NNNNGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG1010.12794365412143247No Hit
NNNAGNGGCTGGGTGACTTGGTGAATGTTGGAGAGGCACATCTATGTGTA1000.12667688526874502No Hit
NNNAGNCCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG950.12034304100530775No Hit
NNNAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG940.11907627215262032No Hit
NNNNGNCCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG940.11907627215262032No Hit
NNTAGNCTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC940.11907627215262032No Hit
NNNAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT930.11780950329993287No Hit
NNNAGNCCAGCTTTGNATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC930.11780950329993287No Hit
NATAGACACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG930.11780950329993287No Hit
NNNAGNCTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC900.1140091967418705No Hit
NNNAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT890.11274242788918305No Hit
NNTAGNTTAGGGGTGACGGACTAGCCCTTGGCCTCAGTTTCCCCTCCTTC880.1114756590364956No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGCATGGAGAAGGGTCTATGTGTAGA880.1114756590364956No Hit
NNNAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC870.11020889018380817No Hit
NNTAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT870.11020889018380817No Hit
NNTAGNCCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG870.11020889018380817No Hit
NNTAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG860.10894212133112072No Hit
NNNAGNGGCTGGGTGACTTGGTGAATCATACCTCCAAGCAGATCTGTGTT850.10767535247843327No Hit
NNNNGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC840.1064085836257458No Hit
NNNAGNCTGGCGGTGATTGCTTTCCTGACTTGGCCTCAGTTTCCCCTCCT840.1064085836257458No Hit
NNNAGNGTTTGCCCTTCCTTCCCTCCAAGCAGATCTGTGTTCTATGTGTA830.10514181477305835No Hit
NNTAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT830.10514181477305835No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGCATGGAGAAGGGTCTATGTGTAGA820.1038750459203709No Hit
NNNNGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT820.1038750459203709No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGCATGGAGAAGGGTCTATGTGTAGA810.10260827706768347No Hit
NNNAGNCACACCCAANTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG800.10134150821499602No Hit
NATAGAGGCTGGGTGACTTGGTGAATGTTGGAGAGGCACATCTATGTGTA790.10007473936230857No Hit
NNTNGNGGCTGGGTGACTTGGTGAATGTTGGAGAGGCACATCTATGTGTA790.10007473936230857No Hit
NNTAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG790.10007473936230857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)