FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71372
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG1415019.825701955949114No Hit
CATAGAGAGATTTTGGGCTGAGACGATAATGCCGCATATCTACAACTATC61148.566384576584655No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGATAGGGTT30994.342038894804685No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTTTCTAGATATACAACAA29004.063218068710419No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGACAGCGTT26793.7535728296811075No Hit
CATAGAAACCCCAAAGGCTATGTTCATTCTATGCCTTCATTCTGCCTGCA19282.7013394608529957No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTTTCTAGATATACACTCA18392.5766406994339515No Hit
CATAGACACCATGCCTGGCTAATTTTTTGCGTTTTTAGTAGAGATGGGGT18022.5247996413159224No Hit
CATAGACCTGGGCTTAGGGGCTACACATGGCTATGCAATCTATGAGACCA13661.9139158213304936No Hit
CATAGAACTTCACAAGGGCTTTAGGGGCTACACATGGCTATGCAATCTAT12791.7920192792691814No Hit
CATAGACCTGGGCTTAGGGGCTATACATGGCTATGCAATCTATGAGACCA12641.7710026340861964No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGGGCAAGCAATGACAG9341.308636440060528No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTTTCTAGATATACAATCA9031.2652020400156925No Hit
CATAGATGTCCATCATGTGGAAGCTGGAGAGTCACTTCTTTTGATAATTC7461.0452278204337835No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGGGGAAAGGATTCTAT7161.0031945300678138No Hit
CATAGAACCTCTTTTGGGGCAAAGTTATATACCCACATGTATTAGTTTGC6960.9751723364905004No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGTGGTGTGGTG6460.9051168525472174No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTTGTAGAGACAGAGTT6300.8826990976853669No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAACCTGTCTCTACT6000.8406658073193969No Hit
CATAGATGCCCTTTTTGTGTACTTCCACACTGACGACATGGTTCTACACA5430.7608025556240542No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTTAGCATGTCTATGAGAC5000.7005548394328308No Hit
CATAGACCTGGGCTTAGGGGCTATACATTCAGGAGAAGTGGCATCTATGT4730.6627248781034579No Hit
CATAGACACCATGCCTGGCTAATTTTAAAATTAGTACCTATAATCTCAGC4420.6192904780586224No Hit
CATAGATGCCCTTTTTGTGTACTTCCACACTGACTGATACAGGAAGGCCT4090.5730538586560555No Hit
CATAGATGCCCTTTTTGTGTACTTCCATGGTTCTACACATAGATGCCCTT3930.550636103794205No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGACAGTCTATGAGACC3780.5296194586112201No Hit
CATAGACACCATGCCTGGCTAATTTTTTGTATTAATCCCAGCTACTCAGG3310.463767303704534No Hit
CATAGACACCATGCCTGGCTAATTTTTAAAAAGTAGCTGGGCATGGTGGC3170.44415176820041474No Hit
CATAGAGCCAAGGAAAGTTGAATGGAGACAGCCGCTCACCTCTATGAGAC2800.3923107100823852No Hit
CATAGAGAGATTTTGGGCTGAGACGATGAGGTTTTCTAAATATACAATCA2790.3909096004035196No Hit
CATAGAAACCCCAAAGGCTATGTTCAAAATGGTCAAACAGGAGGTCTATG2780.38950849072465393No Hit
CATAGACAGGGAGGCGTATAAGTGATGGAGACAGCCGCTCACCTCTATGA2760.3867062713669226No Hit
CATAGATCCCATCCAATAATAATGAACAAGCAATGGGGAAAGGATTCTAT2730.3825029423303256No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTTCCTTCTGCTGGCTATTCTATG2730.3825029423303256No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCTATCTGTTATTCAAAGGTTTT2460.3446729810009528No Hit
CATAGACACCATGCCTGGCTAATTTTTAAATATTTTGTAGAGACCGGGTT2400.33626632292775877No Hit
CATAGATGCCCTTTTTGTGTACTTCCAACACTGACTGATACAGGAAGGCC2340.3278596648545648No Hit
CATAGATTCTGAAGAAATCAGATAGAGAGAAACATACCTGATTGGCCATT2270.3180518971025052No Hit
CATAGACACCATGCCTGGCTAATTTTGTATTTTTAGTAGAGACAGGGTTT2220.3110463487081769No Hit
CATAGACCTGGGCTTAGGGGCTATACGTGAATGTGGATCAGAACTTCTAT2110.2956341422406546No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTGGTGCACATGCC2090.2928319228829233No Hit
CATAGACACCATGCCTGGCTAATTTTTTTGTGTTTTTGGTAGAGATGGGG1940.27181527769993835No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCCCATCTCTA1930.2704141680210727No Hit
CATAGACACCATGCCTGGCTAATTTTTTGTATTTTTAGTAGAGATGGGGT1920.26901305834220707No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGGGAAACATGTCTATAT1810.25360085187468473No Hit
CATAGATGTCAAGAGTCTAATTCCTGCTCTATGTCTGTTATTCAAAGGTT1790.25079863251695345No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGCGTATTCTAAATATACAATCA1760.24659530348035644No Hit
CATAGATTGGAACTTTGCCAGCCTTATCCCCAGAAATGTTAGCATGTCTA1720.24099086476489381No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGACAGGGTT1720.24099086476489381No Hit
CATAGACCTTTGGGTTCATATCATCTGGACACTGACTGATACAGGAAGGC1610.22557865829737153No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTATCCCCAGAAATGTTAGCATGT1480.20736423247211794No Hit
CATAGATCCCATCCAATAATAATGAGCAGAATTACCTCTGGATGGTCTAT1460.2045620131143866No Hit
CATAGACCTTTGGGTTCATATCATCTGGAGACAGCCGCTCACCTCTATGA1430.2003586840777896No Hit
CATAGAAACCCCAAAGGCTATGTTCATTCAATGCCTTCATTCTGCCTGCA1170.1639298324272824No Hit
CATAGAACCTCTTTTGGGGCAAAGTTCCAATATACGTGAATGTGGATCAG1150.1611276130695511No Hit
CATAGACAGGGAGGCGTATAAGTGATGAAATCTCTACTAAAAATACAAAA1130.15832539371181978No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAACCCCGTCTCTAC1100.15412206467522277No Hit
CATAGATCATGGGACTTCAGCATGGCTAATTTTTGTATTTTTAGTAGAGA1090.1527209549963571No Hit
CATAGACACCATGCCTGGCTAATTTTTTGTATTTTTAGTAGAGACGGGGT1070.1499187356386258No Hit
CATAGAGCAACAGGGACTCTCATTCACAATTGCTTCAAAGAGAATAAAAT1060.14851762595976015No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATAACGAAACACGGACTATA980.13730874852883485No Hit
CATAGACCTTTGGGTTCATATCATCTGGCTACACATGGCTATGCAATCTA970.13590763884996918No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTTATCCCCAGAAATGTTA950.13310541949223786No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCCCGTCTCTA930.13030320013450652No Hit
CATAGAGAGATTTTGGGCTGAGACGAAGTCTCTGCTACCGTAGTGTTGAT930.13030320013450652No Hit
CATAGATGTCCATCATGTGGAAGCTGCCTTCCTAACCATGGGCATATCTA930.13030320013450652No Hit
CATAGACACCATGCCTGGCTAATTTTTTGTATTTTTCGTAGAGACAGGGT890.12469876141904389No Hit
CATAGATCCCATCCAATAATAATGAGCAAGCAATGACAATGGGCAAGCAA890.12469876141904389No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTGTACAAGCAATGGGGAAAGGAT860.12049543238244691No Hit
CATAGACACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGATGGGGTT840.11769321302471558No Hit
CATAGAAAACAGAGTCACAGTCCCCAGTAATGTTAGCATGTCTATCCCCA830.11629210334584991No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTCTGCAGATTTGGACCTGCG820.11489099366698424No Hit
CATAGAACTTCACAAGGGCTTTAGGGCTACACATGGCTATGCAATCTATG800.11208877430925292No Hit
CATAGATTGGAACTTTGCCAGCCTTACCAACATGGAGAAACCCCATCTCT780.1092865549515216No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTCGCAGATTTGGACCTGCG780.1092865549515216No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCCTGTCTCTA760.10648433559379028No Hit
CATAGAGGATCCCATCTGAGTTGGTCTCATAGAATCCTTTCCCTCACTCT750.10508322591492461No Hit
CATAGAGAGATTTTGGGCTGAGACGATGGGGTTCTCTAAATATACACTCA740.10368211623605897No Hit
CATAGATCATGGGACTTCAGCATGGCCAACGTGGCGAAACCCTGTCTCTA720.10087989687832764No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)