FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138331
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA3671526.541411541881427No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA103377.472656165284716No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGAATTACAGGTGCCCGCCAC40812.950170243835438No Hit
CATAGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA33642.431848248042738No Hit
CATAGAGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT28232.04075731397879No Hit
CATAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA21741.5715927738540167No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT20261.4646030174002935No Hit
CATAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA20251.463880113640471No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG18911.367011009824262No Hit
CATAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT18731.353998742147458No Hit
CATAGAGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA16651.2036347601043873No Hit
CATAGATTTAAAATAAAATTCCTGGATAGATCACCCTGCTTATCAAACAT13991.0113423599916143No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT13650.986763632157651No Hit
CATAGATTGCATAGCCATGTGTAGCCATGCTGAAGTCCCATGATCTATGT11700.845797398992272No Hit
CATAGAGGTGAGCGGCTGTCTCCAAACTGGACAATGAGAGGATCTATGTG11660.8429057839529822No Hit
CATAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT8070.5833833341767211No Hit
CATAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA7520.5436236273864861No Hit
CATAGAATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG7000.5060326318757185No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC6680.4828997115613998No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCCTGCTAT5320.384584800225546No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG5260.3802473776666112No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTGTCAAATTTGTTAAAAGAGG4970.3592831686317601No Hit
CATAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG4630.3347044407977966No Hit
CATAGACTGTCATTGCTTGCCCATTGCTTGTTTTTCTCAGGTTTGTCAAA4570.33036701823886183No Hit
CATAGAGGTGAGCGGCTGTCTCCACTAAAAATACAAAAATTAGCCAGGCA4370.3159089430424128No Hit
CATAGAGGTGAGCGGCTGTCTCCAGTGTACTGGGGACTGTGACTCTGTTT4330.31301732800312293No Hit
CATAGACCCACTTTCGTGACTTTGTGAGGAGCAGGAATTAGACTCTTGAC4300.3108486167236556No Hit
CATAGAGTTCAAATGGCCAATCAGGTCTCATAAATTCAACAGGCTTTCAT4220.305065386645076No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCCTGCCAC4020.29060731144862684No Hit
CATAGACTGTCATTGCTTGCCCATTGCTCATTATTATTGGATGGGATCTA3880.2804866588111125No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGTCCGCGCAGAGCCTTCAGGTCAGA3760.27181181369324303No Hit
CATAGACTGTCATTGCTTGCCCATTGATAGCCATGCTGAAGTCCCATGAT3750.27108890993342055No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGCACCAC3740.2703660061735981No Hit
CATAGAAAGAGACAGAAGAGAAGGTCATTTAGCCATGCTGAAGTCCCATG3670.26530567985484094No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCCGCCAC3400.24578727833963462No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTGCAGGTGTGTGCCAC3400.24578727833963462No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTGCAGGCGCATGCTTG3330.24072695202087746No Hit
CATAGATATGCCCATGGTTAGGAAGGAACTTTGCCCCAAAAGAGGTTCTA3190.2306062993833631No Hit
CATAGAAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT3170.2291604918637182No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTGTCAGGTTTGTCAAAGATCA3150.2277146843440733No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGCGCCAC3130.2262688768244284No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGTACCACAGGCATGTGCCAC3110.22482306930478343No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTGCCAC3110.22482306930478343No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCACC2950.21325660914762418No Hit
CATAGACCATCCAGAGGTAATTCTGCTCATTATTATTGGATGGGATCTAT2890.20891918658868947No Hit
CATAGATGGAGAGAGTGAGGGAAAGGATGCCATGCTGAAGTCCCATGATC2830.20458176402975473No Hit
CATAGACATGCTAACATTTCTGGGGATACTGGGGACTGTGACTCTGTTTT2560.18506336251454844No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGAGATTATAGGTACTAATTTT2410.1742198061172116No Hit
CATAGATTTAAAATAAAATTCCTGGATAGATCAGTGTGCCATGCTGAAGT2320.16771367227880954No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGTGCACCAC2320.16771367227880954No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG2260.1633762497198748No Hit
CATAGAGCAGAAAACCTTTGAATAACAGAGCAGGAATTAGACTCTTGACA2210.1597617309207625No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCCACCAC2210.1597617309207625No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA2200.15903882716094006No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACGACAGCCCGGCTACTG2180.15759301964129516No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCGCCCTCCAC2080.1503639820430706No Hit
CATAGAATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT2070.14964107828324816No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCCCACCAC2060.14891817452342568No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCGCCCGCCAC1970.1424120406850236No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA1880.13590590684662152No Hit
CATAGACTGTCATTGCTTGCCCATTGACTGTCATTGCTTGCTCATTATTA1780.12867686924839697No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGGTCCGCGCAGAGCCTTCAGGTCA1680.12144783165017241No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA1650.11927912037070505No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTAATACAAAAAATTAG1610.11638750533141523No Hit
CATAGAGGTGAGCGGCTGTCTCCATCTATGAGACCAACTCAGATGGGATC1600.11566460157159278No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGCCCGCCAC1580.11421879405194788No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCACATGCCAC1560.11277298653230297No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGCCA1540.11132717901265804No Hit
CATAGAGGTGAGCGGCTGTCTCCACTAAAAATACAAAAAATTAGCCAGGC1530.11060427525283559No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCATGCGCCAC1530.11060427525283559No Hit
CATAGAGGTGAGCGGCTGTCTCCACTAAAAATACAAAAATTAGCCATGCT1520.10988137149301314No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCGGAGCCTTCAGGTCA1500.10843556397336822No Hit
CATAGATGGAGAGAGTGAGGGAAAGGATTCTATGAGACCAAGCCATGCTG1480.10698975645372331No Hit
CATAGATATGCCCATGGTTAGGAAGGCAGCTTCCACATGATGGACATCTA1480.10698975645372331No Hit
CATAGAGGTGAGCGGCTGTCTCCAGCACAAGAGCCAGCTCCTTGCGAGGC1470.10626685269390086No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA1470.10626685269390086No Hit
CATAGAGGTGAGCGGCTGTCTCCATGATCTATGTGTAGAACGCCATGCTG1440.10409814141443349No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)