FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35625
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG1716648.18526315789474No Hit
CATAGAGAATTTGCACACCACGGGACATGGATGAAGCTGGATCTATGAGA12093.3936842105263163No Hit
CATAGATGTCTCCCAGGCTAGAGTGAGCCCTTGTCCCTTTCTATGAGACC7021.9705263157894737No Hit
CATAGATTCTCCCTTGTGCTTTTGGTACACTGACGACATGGTTCTACACA6961.953684210526316No Hit
CATAGACTTGATGCCCAGGAAACCTCCTGCCCCTGCAGGCTGAAAGTGCC5821.6336842105263156No Hit
CATAGACCACACCCAAACACCACGTACACTGACGACATGGTTCTACACAT5431.5242105263157895No Hit
CATAGATCATGGGACTTCAGCATGGTGGGTGCCTGATTGTTCTATGAGAC4151.1649122807017545No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCACTGACGACATGGTTCTACACAT3801.0666666666666667No Hit
CATAGAGAATTTGCACACCACGGGACCATGCTCACACCTTGATCTATGAG3731.0470175438596492No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACATGGGCAAC2880.8084210526315789No Hit
CATAGACCTTCCTGTTGGCCACACAGAATTAGCGGAGAGTGAGAACTCTA2250.631578947368421No Hit
CATAGACTTGATGCCCAGGAAACCTCACACTGACGACATGGTGGGTGCCT2240.6287719298245614No Hit
CATAGAGGGTGTTTTTCGGTGTTACAACCAATGCTCTTTCATCTATGAGA2180.6119298245614034No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAATCAGGGTTGGTTCTATGAGACC2120.5950877192982456No Hit
CATAGAATGCAGGAGAATATACACAGGTTGCAGTGAGCCAAGATTGTGCC1960.5501754385964912No Hit
CATAGAACAAGGCCAGAATTACCCTGGGTGAGAAATGATGATGGCTCTAT1780.4996491228070175No Hit
CATAGAGGGTGTTTTTCGGTGTCACAACCAATGCTCTTTCATCTATGAGA1770.49684210526315786No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGCAACAGAACAACAACATCTATG1640.4603508771929825No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTCTATGAGACCAAGTCAGCTT1610.4519298245614035No Hit
CATAGAATCAAAATAGTACCCTTGCCTCTACACTCAGACATGTTTCCTAG1530.42947368421052634No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGGAACAGAACAACAACATCTATG1380.3873684210526316No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAGTGAGCCCTTGTCCCTTTCTATG1310.36771929824561406No Hit
CATAGAGGGTGTTTTTCGGTGTCACACACTGACGACATGGTTCTACACAT1180.3312280701754386No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTCTGCAGATTTGGACCTGCG1150.32280701754385965No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCCAGGCCTGAGATTGCTGCTAT1030.28912280701754384No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACCACGGGACATGGATGAAGC1030.28912280701754384No Hit
CATAGATCATGGGACTTCAGCATGGCGGCGTTTGCAGATTTGGACCTGCG1020.28631578947368425No Hit
CATAGACAGCTCACAGACTGCATACTACACTGACGACATGGAGACAGCCG940.263859649122807No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTCGCAGATTTGGACCTGCG930.26105263157894737No Hit
CATAGAACAAGGCCAGAATTACCCTGGGAGAGGCTGTTTGAGATCTATGA910.2554385964912281No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGTG860.24140350877192981No Hit
CATAGAACAAGGCCAGAATTACCCTGTGCTCATACAGAATTAGATCTAGC860.24140350877192981No Hit
CATAGATTAGTTCTTATTCTTGGTATGGCACACACTCAGACATGTTTCCT830.23298245614035087No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCCGCG790.2217543859649123No Hit
CATAGATCTGTGGCTCCCGATAAATGTGCTCATACAGAATTAGATCTAGC720.20210526315789473No Hit
CATAGATTCTCCCTTGTGCTTTTGGTACACTGACGACATGGTGGGTGCCT720.20210526315789473No Hit
CATAGACAGGAGGAGGAAGAAGAGGATTGCTTAAACCTGGGAGGCAGAGG710.19929824561403506No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCGGATTTGGACCTGCG690.1936842105263158No Hit
CATAGAACCCAAAGAGTGAGCAGCAGACGACATGGTTCTACACATAGAAC660.18526315789473682No Hit
CATAGAGCTATCAGTATACTCCCACCATGACGACATGACTCTTCACTATG660.18526315789473682No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAACCCAAAGAGTGAGCCCTTGTCC630.1768421052631579No Hit
CATAGACACTGATAGGTTCCAACTTTCCTGGGAGAGGCTGTTTGAGATCT620.17403508771929824No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGGCCTGCG620.17403508771929824No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTCTGACGACATGGTGGGTGCC620.17403508771929824No Hit
CATAGATGTCTCCCAGGCTAGAGTGCGCCCTTGTCCCTTTCTATGAGACC620.17403508771929824No Hit
CATAGAACTATGTCAAGAGAATTTTGCACACCACGGGACCATGCTCACAC600.16842105263157894No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCTGACGACATGGTTCTACACATAG580.16280701754385965No Hit
CATAGACCTTCCTGTTGGCCACACAGGCAGCAAAGAGCCAGATCTTGCAT550.1543859649122807No Hit
CATAGATCATGGGACTTCAGCATGGTGGTGTTTGCAGATTTGGACCTGCG540.15157894736842106No Hit
CATAGAACCCAAAGAGTGAGCAGCAGTTCTACACATAGAACCCAAAGAGT510.14315789473684212No Hit
CATAGAGGGCCAGAAGTTTGTTTACCTATAGAGGCCTGAGATTGCTGCTA510.14315789473684212No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGACTTGGACCTGCG500.14035087719298245No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGCTTGCAGATTTGGACCTGCG500.14035087719298245No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCCGGCTTGGCAGGTCTCAAGAG500.14035087719298245No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTCGGACCTGCG500.14035087719298245No Hit
TAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCGAG490.1375438596491228No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTCTATGAGACCAAGGGGACAT490.1375438596491228No Hit
CATAGACTTGATGCCCAGGAAACCTCTAAAAAGATGCTCTTCCTCATTCG490.1375438596491228No Hit
CATAGATCTTTGGTAGATATGGGGTCTCCAGGCTAGCACACCTGGTTGGG480.13473684210526315No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCATGGTTCTACACATAGAACCCAA480.13473684210526315No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGGTTTGGACCTGCG470.1319298245614035No Hit
CATAGAACCCAAAGAGTGAGCAGCAGAGAGTGAGCCCTTGTCCCTTTCTA460.12912280701754386No Hit
CATAGAACAATGTAATAAGCATCGGAGGACACTGACGACATGGTTCTACA430.12070175438596491No Hit
CATAGAACCCAAAGAGTGAGCAGCAGACATGGTTCTACACATAGAACCCA430.12070175438596491No Hit
CATAGAGAATTTGCACACCACGGGACATGGATGAAGCTGGACCTATGAGA420.11789473684210526No Hit
CATAGAACCCAAAGAGTGAGCAGCAGACACTGACGACATGGTTCTACACA420.11789473684210526No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATCTGGACCTGCG410.11508771929824563No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGATCTGCG400.11228070175438595No Hit
CATAGACAGGAGGAGGAAGAAGAGGAGGGACATGGATGAAGCTGGATCTA390.10947368421052632No Hit
CATAGAGGGTGTTTTTCGGTGTCACACTGACGACATGGTTCTACACATAG380.10666666666666667No Hit
CATAGAACCCAAAGAGTGAGCAGCAGCCCAAAGAGTGAGCCCTTGTCCCT370.10385964912280701No Hit
CATAGAGGGTGTTTTTCGGTGTCACAGGCAGTCTCAAACGTTGTGTCTAT370.10385964912280701No Hit
CATAGAACAGACACAAATCTCAAAGCTTCTACACTGACGACATGGTTCTA370.10385964912280701No Hit
CGTAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG360.10105263157894737No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAAATTTGGACCTGCG360.10105263157894737No Hit
CATAGAGCTTCTTTCTGTTCATATTGGGGAACACTGACGACATGGTTCTA360.10105263157894737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)