FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35625
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA1593744.73543859649123No Hit
CATAGATCCAGCTTCATCCATGTCCCGTGGTGTGCAAATTCTCTATGTGT11843.3235087719298244No Hit
CATAGAAAGGGACAAGGGCTCACTCTAGCCTGGGAGACATCTATGTGTAG11503.2280701754385968No Hit
CATAGAAAGGGACAAGGGCTCACTCTTTGGGTTCTATGTGTAGAACCATG8902.4982456140350875No Hit
CATAGAGCTAGATCTAATTCTGTATGAGCACAAGGGAGAATCTATGTGTA6541.8357894736842104No Hit
CATAGAACAATCAGGCACCCACCATGCTGAAGTCCCATGATCTATGTGTA4791.3445614035087718No Hit
CATAGAGGCGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA4591.288421052631579No Hit
CATAGACTCACTCTGTCACCCAAGCTGGAGTGCAGTGGCGTGCTCTTGGC3871.0863157894736841No Hit
CATAGATCAAGGTGTGAGCATGGTCCCGTGGTGTGCAAATTCTCTATGTG3721.0442105263157895No Hit
CATAGATGTTGTTGTTCTGTTGCCCATGTCGTCAGTGTAGAAGCTTTGAG2570.7214035087719298No Hit
CATAGAACCAACCCTGATTCTGCTGCTCACTCTTTGGGTTCTATGTGTAG2440.6849122807017544No Hit
CATAGATGAAAGAGCATTGGTTGTAACACCGAAAAACACCCTCTATGTGT2280.64No Hit
CATAGAGTTCTCACTCTCCGCTAATTCTGTGTGGCCAACAGGAAGGTCTA2080.583859649122807No Hit
CATAGAACAATCAGGCACCCACCATGTCGTCAGTGTGAGGTTTCCTGGGC2080.583859649122807No Hit
CATAGAAAGGGACAAGGGCTCACTCTGCTGCTCACTCTTTGGGTTCTATG1710.48No Hit
CATAGAGCCATCATCATTTCTCACCCAGGGTAATTCTGGCCTTGTTCTAT1690.4743859649122807No Hit
CATAGATGTTGTTGTTCTGTTGCCCAATATGAACAGAAAGAAGCTCTATG1630.4575438596491228No Hit
CATAGATGAAAGAGCATTGGTTGTGACACCGAAAAACACCCTCTATGTGT1600.4491228070175438No Hit
CATAGACTCACTCTGTCACCCAAGCTGGAGTGCAGTGGCACAATCTTGGC1560.4378947368421053No Hit
CATAGACTCACTCTGTCACCCAAGCTGACTTGGTCTCATAGAGAAGCTTT1460.4098245614035088No Hit
CATAGAACTGAAACTAGGAAACATGTCTGAGTGTAGAGGCAAGGGTACTA1410.3957894736842106No Hit
CATAGATGTTGTTGTTCTGTTCCCCAATATGAACAGAAAGAAGCTCTATG1330.37333333333333335No Hit
CATAGACTCACTCTGTCACCCAAGCTGGAGTGCAGTGGCGCGCTCTTGGC1290.3621052631578947No Hit
CATAGATAGCAGCAATCTCAGGCCTGGGAGACCCCATATCTACCAAAGAT1020.28631578947368425No Hit
CATAGATCCAGCTTCATCCATGTCCCGTGGTGTGCAAAATTCTCTTGACA980.2750877192982456No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG860.24140350877192981No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA850.2385964912280702No Hit
CATAGATCTCAAACAGCCTCTCCCAGGGTAATTCTGGCCTTGTTCTATGT830.23298245614035087No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA810.22736842105263158No Hit
CATAGAGGTGAGCGGCTGTCTCCATGTCGTCAGTGTAGTATGCAGTCTGT800.2245614035087719No Hit
CATAGAGCTAGATCTAATTCTGTATGAGCACAGGGTAATTCTGGCCTTGT800.2245614035087719No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCGGAGCCTTCAGGTCA800.2245614035087719No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA790.2217543859649123No Hit
CATAGAAAGGGACAAGGGCTCACTCTTTGGGTTCTGCTGCTCACTCTTTG770.21614035087719297No Hit
CATAGAACTGAAACTAGGAAACATGTCTGAGTGTGTGCCATACCAAGAAT770.21614035087719297No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA750.21052631578947367No Hit
CATAGAACAATCAGGCACCCACCATGTCGTCAGTGTACCAAAAGCACAAG750.21052631578947367No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGTCCGCGCAGAGCCTTCAGGTCAGA720.20210526315789473No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGCCA690.1936842105263158No Hit
CATAGATGTTGTTGTTCTGTTGCCCACTCTTTGGGTTCTATGTGTAGAAC680.19087719298245615No Hit
CATAGAGCTAGATCTAATTCTGTATGAGCACATTTATCGGGAGCCACAGA680.19087719298245615No Hit
CATAGAGGTGAGCGGCTGCCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA650.18245614035087718No Hit
CATAGAAAGGGACAAGGGCTCACTCTCTGCTGCTCACTCTTTGGGTTCTA650.18245614035087718No Hit
CATAGATGGTAAGATGACATAGTGAAGAGTCATGTCGTCATGGTGGGAGT640.17964912280701756No Hit
CATAGAAAGGGACAAGGGCGCACTCTAGCCTGGGAGACATCTATGTGTAG620.17403508771929824No Hit
CATAGAGGTGAGCGGCTGTCCCCACAAGTCCGCGCAGAGCCTTCAGGTCA600.16842105263157894No Hit
CATAGAACAATCAGGCACCCACCATGTCGTCAGAGAAGCTTTGAGATTTG590.1656140350877193No Hit
CATAGACTCACTCTGTCACCCAAGCTGTAGTGCAGGGGCGTGATCTTGTC580.16280701754385965No Hit
CATAGATCTCAAACAGCCTCTCCCAGGAAAGTTGGAACCTATCAGTGTCT570.16No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGCCCGCGCAGAGCCTTCAGGTCA560.15719298245614036No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGGTCCGCGCAGAGCCTTCAGGTCA560.15719298245614036No Hit
CATAGATCAAGGTGTGAGCATGGTCCCGTGGTGTGCAAAATTCTCTTGAC550.1543859649122807No Hit
CATAGATCTCAAACAGCCTCTCCCAGAGGACAAGACAACAACAAAACCTA550.1543859649122807No Hit
TATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA540.15157894736842106No Hit
CATAGATAGCAGCAATCTCAGGCCTCTATAGGTAAACAAACTTCTGGCCC510.14315789473684212No Hit
CGTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA500.14035087719298245No Hit
CATAGATCCAGCTTCATCCATGTCCCTGAAAAGAACACGAATGAGGAAGA490.1375438596491228No Hit
CATAGATCCAGCTTCATCCATGTCCCCTTGGTCTCATAGAGAAGCTTTGA480.13473684210526315No Hit
CATAGAAAGGGACAAGGGCTCACTCTTTGGGTTCTATGTGTAGAACTGCT450.12631578947368421No Hit
CATAGAACAATCAGGCACCCACCATGTCCCCATTGCTCTTGAGACCTGCC440.12350877192982457No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGCGCCTTCAGGTCA430.12070175438596491No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGGGCCTTCAGGTCA430.12070175438596491No Hit
CATAGACACAACGTTTGAGACTGCCTGTGACACCGAAAAACACCCTCTAT410.11508771929824563No Hit
CATAGATCTAGCTTCATCCATGTCCCGTGGTGTGCAAATTCTCTATGTGT410.11508771929824563No Hit
CATAGGGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA400.11228070175438595No Hit
CATAGAGATCTTCTCGGCTTAGCGGCTGTCTCCACAAGTCCGCGCAGAGC400.11228070175438595No Hit
CATAGATAGCAGCAATCTCAGGCCTCTTCTTCCTCCTCCTCTTCTTCCTC400.11228070175438595No Hit
CATAGATCCAGCTTCATCCATGTCCCTCCTCTTCTTCCTCCTCCTGTCTA400.11228070175438595No Hit
CATAGATCTCAAACAGCCTCTCCCAGGACAAACACCCAACCAGGTGTGCT390.10947368421052632No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGTGCAGAGCCTTCAGGTCA380.10666666666666667No Hit
CATAGATCCAGCTTCATCCATGTCCCGTGGTGTGCAAATTCTCTATGTGC360.10105263157894737No Hit
CATAGATCCAGCTTCATCCATGTCCCAGTGTAGTATGCAGTCTGTGAGCT360.10105263157894737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)