FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136302
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNTAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA45213.3168992384557816No Hit
NNNAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA44873.2919546301594984No Hit
NNNNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA42663.1298146762336576No Hit
NATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA41703.059382841044152No Hit
NNTNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA34992.567093659667503No Hit
NNNNGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA11990.87966427491893No Hit
NNNAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA11490.8429810274243958No Hit
NNTAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG11120.8158354242784405No Hit
NNTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA11110.8151017593285499No Hit
NNNAGNGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA10910.8004284603307361No Hit
NNNAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG10800.7923581458819386No Hit
NNNNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG10200.7483382488884976No Hit
NATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG9080.666167774500741No Hit
NNTAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG8580.6294845270062067No Hit
NNTNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG8370.6140775630585025No Hit
NNNAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG8300.6089419084092677No Hit
NNNNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG7880.5781279805138589No Hit
NATAGAGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA7150.524570439171839No Hit
NNNAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT7040.5165001247230415No Hit
NNTAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT6990.5128317999735881No Hit
NATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG6800.49889216592566504No Hit
NNTNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG6790.49815850097577435No Hit
NNNNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT6130.4497366142829892No Hit
NNNAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT5880.43139499053572217No Hit
NNTAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT5840.4284603307361594No Hit
NATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT5580.40938504203900167No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA5280.3873750935422811No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA5180.3800384440433743No Hit
NNNNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT5070.37196812959457676No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA4940.36243048524599786No Hit
NNTNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT4820.35362650584730965No Hit
NATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT4770.34995818109785626No Hit
NNTAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA4710.3455561913985121No Hit
NNNAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG4690.34408886149873075No Hit
NATAGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA4620.338953206849496No Hit
NNTAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG4580.3360185470499333No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC4470.3279482326011357No Hit
NNNNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG4440.3257472377514637No Hit
NNNAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA4440.3257472377514637No Hit
NNTNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT4440.3257472377514637No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC4290.31474226350310336No Hit
NNNNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA4130.30300362430485245No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA3950.28979765520682016No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3890.285395665507476No Hit
NNTAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3600.2641193819606462No Hit
NATAGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA3560.26118472216108346No Hit
NATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3500.2567827324617394No Hit
NNTNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA3490.2560490675118487No Hit
NNNAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3430.2516470778125046No Hit
NATAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3430.2516470778125046No Hit
NNTNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3330.24431042831359776No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3320.24357676336370704No Hit
NNNNGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3150.23110445921556544No Hit
NNNNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3000.2200994849672052No Hit
NNTAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2770.20322519111971946No Hit
NNNAGNCCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2740.2010241962700474No Hit
NATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG2700.19808953647048466No Hit
NNNAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2630.19295388182124987No Hit
NNTNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG2620.1922202168713592No Hit
NNNNGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2560.1878182271720151No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2280.16727560857507595No Hit
NNNAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2190.16067262402605978No Hit
NNNNGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT2180.1599389590761691No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2140.15700429927660636No Hit
NNTAGNGTCTCAATTATTCATCTCAACATGCTGAGAGAAAACTACCCCAG2120.15553696937682499No Hit
NNTAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2100.1540696394770436No Hit
NNNAGNTACGATCATNAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2070.15186864462737157No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2020.14820031987791815No Hit
NNTAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1880.1379290105794486No Hit
NNNNGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1810.1327933559302138No Hit
NNNAGNGTCTCAATTATTCATCTCAACATGCTGAGAGAAAACTACCCCAG1790.13132602603043242No Hit
NNNAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1720.12619037138119762No Hit
NNNAGNCACCATTGGNGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1700.12472304148141626No Hit
NNNNGNGTCTCAATTATTCATCTCAACATGCTGAGAGAAAACTACCCCAG1670.12252204663174421No Hit
NNNAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1650.12105471673196284No Hit
NNTAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1650.12105471673196284No Hit
NNNAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1650.12105471673196284No Hit
NATAGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1640.12032105178207216No Hit
NNNNGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1610.1181200569324001No Hit
NNTAGNCACACCCAACTCTGCTCAGTGTACCAGTGTAGCAGACCATCTTC1550.11371806723305601No Hit
NNTAGNGTCTCAATTATTCATCTCAACATGCTTTCTGGGTAGCTGCGACC1510.11078340743349328No Hit
NNNAGNGTCTCAATTATTCATCTCAACATGCTTTCTGGGTAGCTGCGACC1480.10858241258382123No Hit
NNTNGNGTCTCAATTATTCATCTCAACATGCTGAGAGAAAACTACCCCAG1480.10858241258382123No Hit
NNNAGNCACACCCAACTCTGCTCAGTGTACCAGTGTAGCAGACCATCTTC1450.10638141773414916No Hit
NNNAGNTGTTTGAAANTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1440.10564775278425849No Hit
NNTAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1390.10197942803480506No Hit
NNNNGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1380.10124576308491438No Hit
NATAGACACACCCAACTCTGCTCAGTGTACCAGTGTAGCAGACCATCTTC1380.10124576308491438No Hit
NATAGAGTCTCAATTATTCATCTCAACATGCTGAGAGAAAACTACCCCAG1370.1005120981350237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)