FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171458
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA5844134.0847321209859No Hit
CATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG95935.594956199185806No Hit
CATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG74044.318258698923351No Hit
CATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT56023.2672724515624822No Hit
CATAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC47292.758109857807743No Hit
CATAGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA42502.4787411494360136No Hit
CATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG39322.2932729881370366No Hit
CATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG36622.1358000209963954No Hit
CATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT29911.7444505359913216No Hit
CATAGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA24531.4306710681333037No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGACACTGGGGGCACAAAGTTCT21801.271448401357767No Hit
CATAGACACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG19441.1338053634126142No Hit
CATAGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG18521.0801479079424698No Hit
CATAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT12830.7482882105238601No Hit
CATAGACTTACTGCCCTCACTCCGTCTGCATAGCCACATCTATGTGTAGA12790.745955277677332No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT10740.6263924692927714No Hit
CATAGAGGGGAGACACTGGGGGCACAAAGTTCTATGTGTAGAACCATGTC10360.6042296072507553No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTTACGGTAGCAAAGCT10080.5878990773250592No Hit
CATAGACTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC10030.5849829112668992No Hit
CATAGACCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG8580.5004140955802587No Hit
CATAGACCAGCTTTGCATGAGTGTGGCATGGAGAAGGGTCTATGTGTAGA8560.4992476291569947No Hit
CATAGATGTGAGCACTACTGATAATCCCTGATGTTTTAAAATGAGCACCT7950.4636704032474425No Hit
CATAGACTTACTGCCCTCACTCAGTCTGCATAGCCACATCTATGTGTAGA7930.4625039368241785No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGGGGAGACACTGGGGGCACAAA6100.35577225909552196No Hit
CATAGAGTTATTCAGAGAGAGGGGAAATGATGTGGCATGGAGAAGGGTCT5700.33244293063024183No Hit
CATAGAGGGGAGACACTGGCTATAAGAACTTGGTCTCATAGAGGGGAGAC5200.30328127004864164No Hit
CATAGACTTACTGCCCTCACTCCGTCTACGGTAGCAAACACTCAAAACAG4990.29103337260436957No Hit
CATAGACCTCCTTTCCTCCACGGCAAAGCTGCTATGGGATCTTCTATGTG4790.2793687083717295No Hit
CATAGACTGGCGGTGATTGCTTTCCTACTTGGCCTCAGTTTCCCCTCCTT4770.27820224194846555No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGACTTGGTCTCATAGAGGGGAG4660.2717866766205135No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCAAGCAGATCTGTGTTCTATGTGTA4610.26887051056235345No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT4460.2601220123878734No Hit
CATAGATGGTGGGTTCTGGAGAGAGACACTGGGGGCACAAAGTTCTATGT4430.2583723127529774No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTAGCAGAGACTTGGCCTCAGTTTC4330.25253998063665734No Hit
CATAGACTTTTCCTCCAATATAGCTGCACTTAATCTATGTGTAGAACCAT4120.2402920831923853No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGAGACTTGGTCTCATAGAGGGG3930.22921065217137723No Hit
CATAGACCAGCTTTGCATGAGTGTGGGGAAGTAGGGAATGATTACAAGTT3910.22804418574811325No Hit
CATAGACCAGCTTTGCATGAGTGTGGCCAGCTTTGCATGAATGTTGGAGA3790.22104538720852918No Hit
CATAGAGGGGAGACACTGGCTATAAGAACAGAGACTTGGTCTCATAGAGG3760.21929568757363319No Hit
CATAGATGTGAGCACTACTGATAATCCCTTTTGCTCATCCATTGTTGATC3680.21462982188057717No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTATTTGAGGTAAAACT3670.21404658866894516No Hit
CATAGATTAGGGGTGACGGACTAGCCATGCTGAAGTCCCATGATCTATGT3670.21404658866894516No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCAGACTTGGCCTCAGTTTCCCCTCC3630.21171365582241716No Hit
CATAGAGTCTCAATTATTCATCTCAACATGCCATGCTGAAGTCCCATGAT3530.20588132370609713No Hit
CATAGAGGGGAGACACTGGCTATAAGAATAGAGGGGAGACACTGGGGGCA3500.2041316240712011No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCACAGAGCCTTCAGGTCA3350.19538312589672105No Hit
CATAGATACGATCATAAGGGTGGGGCCTGTGTTCTTTGCATGATCTATGT3310.19305019305019305No Hit
CATAGAGTTATTCAGAGAGAGGGGAAATGATCAGAAGCTCTATGGGCGAA3300.19246695983856105No Hit
CATAGACCAAACACAGAAGACATGGCAGCAGAGACTTGGTCTCATAGACA3030.17671966312449697No Hit
CATAGATTAGGGGTGACGGACTAGCCCAGAGACTTGGCCTCAGTTTCCCC2910.16972086458491292No Hit
CATAGAGGTGAGCGGCTGTCTCCAGAAGTCCGCGCAGAGCCTTCAGGTCA2820.1644717656802249No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA2710.15805620035227286No Hit
CATAGATGGTGGGTTCTGGAGAGAGGGCGAAAGTGATCAGAAGCTCTATG2680.15630650071737684No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGCAGAGACTTGGTCTCATAGAG2670.15572326750574486No Hit
CATAGACCAGCTTTGCATGATGTGGCATGGAGAAGGGTCTATGTGTAGAA2630.15339033465921684No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGTCCGCGCAGAGCCTTCAGGTCAGA2600.15164063502432082No Hit
CATAGACCTGACCTCATGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGG2580.15047416860105683No Hit
CATAGATACGATCATAAGGGTGGGGCTGGGCTCAGTGGCTTATGCCTGTA2530.1475580025428968No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGAGACACTGGGGGCACAAAGTT2460.1434753700614728No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA2320.13531010509862473No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA2310.13472687188699273No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG2280.13297717225209674No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGAGGGGAGACACTGGGGGCACA2250.13122747261720072No Hit
CATAGATGACCTGTTAATCTGCCCCCAGATCACAGAGTGGCCTTGGGCAA2180.1271448401357767No Hit
CATAGATGACCTGTTAATCTGCCCCCACTCAGTCTGCATAGCCACATCTA2180.1271448401357767No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCGCAGAGACTTGGCCTCAGTTTCCC2170.12656160692414467No Hit
CATAGACCAGCTTTGCATGAGTGTGGGCGAAAGTGATCAGAAGCTCTATG2160.12597837371251266No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGCCA2150.1253951405008807No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGGTCCGCGCAGAGCCTTCAGGTCA2120.12364544086598467No Hit
CATAGAGGAGCACCCTGAAGTTAAGATTTATCCAGCATGCCCCTCTATGT2110.12306220765435268No Hit
CATAGACCTGACCTCATGATCTGCCTACCTCAGCCTCCCAGATTGCTGGG2010.11722987553803264No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCGGAGCCTTCAGGTCA1980.11548017590313663No Hit
CATAGAGGGGAGACACTGGCTATAAGAAAGGGGAGACACTGGGGGCACAA1960.11431370947987261No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGGGCCTTCAGGTCA1880.10964784378681659No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA1790.10439874488212857No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTGGCCTCAGTTTCCCCTCCTTCTA1750.10206581203560056No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGGGAGACACTGGGGGCACAAAG1730.10089934561233656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)