FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91737
Sequences flagged as poor quality0
Sequence length150
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA3640639.685187001973034No Hit
CATAGATTGCACATAAAGTCTGATCCCATCTCAAGTCTCACTCTTCTATG51015.560460882740879No Hit
CATAGACTACCACTACCCAGCACAGCTACTTCTCTATGCACTTTGCCACA22062.4047003935162476No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGTGATTACAGGTG20752.261900868787948No Hit
CATAGAGCCTGGCATTTGAGGTAAGGCTTGTGAGGGCTGAAGTCTATGTG17941.9555904378822067No Hit
CATAGACTACCACTACCCAGCACAGGAGCACCCTCTATGTGTAGAACCAT17191.8738349847934856No Hit
CATAGAGAATACAATGAACTGTGATGGCAGTGTAGGGATAGTTTGAGGGT11501.2535836140270555No Hit
CATAGACCATCCTCCCACCTTGACCTCTTAAGTAGCTGGGACTACAGGTG11351.2372325234093116No Hit
CATAGAAGCAGCATTGATCATCCTCCACCCCATCCTACATGTGTCTATGT11221.2230615782072665No Hit
CATAGACTACCACTACCCAGTACAGGAGCACCCTCTATGTGTAGAACCAT10301.1227748890851021No Hit
CATAGAGATGTCAAGAGCAATGCCCAGCCACCCCATCCTACATGTGTCTA7610.829545330673556No Hit
CATAGATCCCAGGCTTTAGAAAATATACCAAGGCTTGTGAGGGCTGAAGT7130.7772218406967745No Hit
CATAGACTACCACCACCCAGTACAGGAGCACCCTCTATGTGTAGAACCAT6060.6605840609568658No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGTG5480.5973598439015882No Hit
CATAGAAGCAGCATTGATCATCCTCCAGTCTGCTCTTTGACTTATGATGG4840.5275951905992129No Hit
CATAGACCATCCTCCCACCTTGACCTCCCGAGTAGCTGGGACTACAGGTG4840.5275951905992129No Hit
CATAGAGATGTCAAGAGCAATGCCCACCCCATCCTACATGTGTCTATGTG4650.5068838091500703No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAAGTGAAGGAGAGGCCAC4580.49925330019512304No Hit
CATAGACTGTTCTTTTAAATTAACACAGTCAGAGAGGCACTTGGTCCAGA4540.49489300936372455No Hit
CATAGACCATCCTCCCACCTTGACCTCCTGAGTAGCTGAGACCACAGGCA4410.4807220641616796No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAGAGTGCTGGAATTATAGGCG4020.4382092285555446No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTATTACTACAGGTG3830.417497847106402No Hit
CATAGAAGGTGATAATCATGACAGCCACCCCATCCTACATGTGTCTATGT3760.4098673381514547No Hit
CATAGACCATCCTCCCACCTTGACCTTCCAAAGTGCTAGGATAATAGGTG3670.4000566837808082No Hit
CATAGAAGGTGATAATCATGACAGCCAACTGATCATCAACAAAGCAAACA3600.3924261748258609No Hit
CATAGAGATGTCAAGAGCAATGCCCATGCTGAAGTCCCATGATCTATGTG3250.35427363005112444No Hit
CATAGAAGCAGCATTGATCATCCTCCATACGGTAGCATTTTAACACAGCA3210.34991333921972595No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAGGTGTAAGGGAAGCCAC2940.3204813761077864No Hit
CATAGAACATCGCACACACATGGAACTACGGTAGCATTTTAACACAGCAT2890.3150310125685383No Hit
CATAGACCATCCTCCCACCTTGACCTCCTGAATAGCTAGGACTAGGTGTG2880.3139409398606887No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAGGTGAAGGGGGAGGCAA2880.3139409398606887No Hit
CATAGATGACATAGACCTTATACCCTCATCACCCAGTACAGGAGCACCCT2840.3095806490292903No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGCA2810.30631043090574145No Hit
CATAGAAGGTGATAATCATGACAGCCAAGCTATATAGAAGTACATGGTGG2790.3041302854900422No Hit
CATAGAAGCAGCATTGATCATCCTCCAGCAGCATTTTAACACAGCATTGA2610.28450897674874914No Hit
CATAGACCATCCTCCCACCTTGACCTCTCAAAGTGCTAGGGTTACAGGCA2490.27142810425455377No Hit
CATAGATCCCAGGCTTTAGAAAATATACCACCCAGTACAGGAGCACCCTC2420.26379759529960645No Hit
CATAGACCATCCTCCCACCTTGACCTCCATCTTGACCTCCCAAAGTGCTG2400.2616174498839073No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA2350.2561670863446592No Hit
CATAGAGATGTCAAGAGCAATGCCCATGCCTAGCCATTTTTGGTTCTATG2350.2561670863446592No Hit
CATAGACCATCCTCCCACCTTGACCTATCCTCCCATCTCAAGTCTCACTC2290.24962665009756152No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA2290.24962665009756152No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGTCCGCGCAGAGCCTTCAGGTCAGA2200.23981599572691498No Hit
CATAGACCATCCTCCCACCTTGACCTCATCCTCCCATCTCAAGTCTCACT2160.2354557048955165No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGCG2140.23327555947981732No Hit
CATAGAGGGACGGAGGCAAGAGGATGGGTTTTTGCCAGATAGCTCTATGT1950.21256417803067465No Hit
CATAGAGATGTCAAGAGCAATGCCCATCCCATCTCAAGTCTCACTCTTCT1950.21256417803067465No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAACAGGTACTGGGATTACAGG1920.20929395990712582No Hit
CATAGACCATCCTCCCACCTTGACCTACGTAGCATTTTAACACAGCATTG1860.2027535236600281No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGCCA1730.18858257845798315No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA1720.18749250575013351No Hit
CATAGAGGTGAGCGGCTGTCTCCACACGTCCGCGCAGAGCCTTCAGGTCA1680.18313221491873508No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG1670.18204214221088547No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAGGCAAAAAAGAAGCAGG1560.1700513424245397No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGGTCCGCGCAGAGCCTTCAGGTCA1530.16678112430099087No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGTGTAGTCTTTTGCAAGTTT1520.16569105159314126No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGAGATTACACACG1510.16460097888529165No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCGGAGCCTTCAGGTCA1500.16351090617744202No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGGGCTGGGATGACACGCA1490.16242083346959243No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGAG1480.16133076076174283No Hit
CATAGAGATGTCAAGAGCAATGCCCGGCCACATTATTTTCTATGTGTAGA1470.1602406880538932No Hit
CATAGAGATGTCAAGAGCAATGCCCAGCCACATTATTTTCTATGTGTAGA1460.15915061534604358No Hit
CATAGAGGTGAGCGGCTGTCTCCATAAGTCCGCGCAGAGCCTTCAGGTCA1430.15588039722249475No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGTTAGGATTACAGGCA1360.14824988826754745No Hit
CATAGACCATCCTCCCACCTTGACCTCCTGAGTAGCTGGAACTACAGGAG1290.14061937931260016No Hit
CATAGACTGTTCTTTTAAATTAACACAGTCAGAGGCACTTGGTCCAGACT1240.13516901577335208No Hit
CATAGACCGGGGGTAGATAGGTGGTTAGTGTGCCACCCCATCCTACATGT1230.13407894306550247No Hit
CATAGAAGCAGCATTGATCATCCTCCAATCCTCCCATCTCAAGTCTCACT1210.13189879764980322No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTAGGATTACAAGTG1180.1286285795262544No Hit
CATAGACTCAAGAAACTTACAATCATGGCAAAAGTCAAAGGTGAAGCCAC1180.1286285795262544No Hit
CATAGACCGGGGGTAGATAGGTGGTTAGTGTAGGGATAGTTTGAGGGTAA1170.12753850681840478No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGCCCGCGCAGAGCCTTCAGGTCA1140.12426828869485594No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGGGCCTTCAGGTCA1130.12317821598700635No Hit
CATAGAGCCTGGCATTTGAGGTAAGGCACTTGGTCCAGACTTTCTATGTG1090.11881792515560788No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA1090.11881792515560788No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAGTAGCTGGGACCACAGGTG1020.1111874162006606No Hit
CATAGACCATCCTCCCACCTTGACCTGCGTCATGAGCCACCATGCCCGGC930.10137676183001405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)