FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66450
Sequences flagged as poor quality0
Sequence length150
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG1408021.188863807373963No Hit
CATAGATCATGGGACTTCAGCATGGCTCAGGTATGTCTTTATTAGCTCTA40566.1038374717832955No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGTAATAACCAGGCCCCTCTATGAG31024.668171557562077No Hit
CATAGACGGATTATTCTCTCCATGGTCTACCAGTTATTAGTTGAATGAGC26363.9668924003009782No Hit
CATAGAGAAGACTCCCTCAACCCACCCACAGCCAGGAAAGAATCTATGAG26053.9202407825432655No Hit
CATAGACTCATTGCACTTTCCACAACTGTGTGCTTGAACCAGATTCTATG15882.3897667419112114No Hit
CATAGATCACGTAGAAACTGGAGAGGCTAATCTGAAATCTTCGTGTTCTA15082.269375470278405No Hit
CATAGAAATGATGCTGAAGGGCCTGCCCTCTCTGCTGTTCCTTCTATGAG15012.2588412340105344No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAAGGAAGTTTCTGTGAGATC13992.105342362678706No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTCTTCCTTTTCTGCCTTCATCTA13872.087283671933785No Hit
CATAGACTCATTGCACTTTCCACAACTGTCCCAACTGTCTCTGAAAAATT12161.8299473288186607No Hit
CATAGAAATGATGCTGAAGGGCCTGTTTCCCCACTCAAATACCATCTATG11231.689992475545523No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAGGAACCATGGGAGGTCTAT10961.6493604213694508No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGCCCCAGTGTGAATACTTCT10891.6388261851015802No Hit
CATAGATCATGGGACTTCAGCATGGAGACAGCCGCTCACCTCTATGAGAC7471.124153498871332No Hit
CATAGACTCATTGCACTTTCCACAACTGTCATTGTCGCTGTCCCATCTAT6320.9510910458991723No Hit
CATAGACGTGACTAAAAGCATGTTGGTGGAAGGGTAAGAGAGGGTCTATG5850.8803611738148984No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGAAGTGATGGGCTAGATCTATGA5460.8216704288939052No Hit
CATAGATGTTTTATCAAAGGCATGCTCATTTATTTTAAAGGACTGGAAGC4990.7509405568096313No Hit
CATAGACCAGCAGACACTCCCATCATACACTGACGCTATACCACACTACA4880.7343867569601205No Hit
CATAGATGTGTAGCCTGGGTCTGACATGCCAGGAATCAATGTTCTATGAG4250.6395786305492852No Hit
CATAGAGCTGTACTGCATCTTTTCTCAAACTGCTGGGGTTGCTCTATGAG4200.6320541760722348No Hit
CATAGAGAAGACTCCCTCAACCCACGCTGTCCTTCTCTGAATCTATGAGA4130.6215199398043642No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGGGGTAAGGCGTACTGGTCTATGA4110.618510158013544No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACGCTATACCACACTACACACT3760.5658389766741911No Hit
CATAGACACAACAGAGAGAACGGGCTGGTGCATTACAGAGAGGAGATCTA3750.5643340857787811No Hit
CATAGACCAGCAGACACTCCCATCATTCAAGAGATCCCACCATTTGCCAT3440.5176824680210684No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGATCTGCG2830.4258841234010534No Hit
CATAGATCAAGGAAAACACCAGCCCACACTGACGCTATACCACACTACAC2820.42437923250564336No Hit
CATAGACCTCCGACAGTTTTTATGCACCTTCATTGTTGCTCTATGAGACC2740.4123401053423627No Hit
CATAGATCAAGGAAAACACCAGCCCACGCTGTCCTTCTCTGAATCTATGA2610.39277652370203164No Hit
CATAGACGGATTATTCTCTCCATGGTCTACACTGACGCTATACCACACTA2510.37772761474793076No Hit
CATAGAAGCTGAGAAGACTTCCTAATGCAGAAGTAGGAAGTATTACCCCT2420.36418359668924005No Hit
CATAGATGTGTAGCCTGGGTCTGACAGTGTTGCAGAAGTAGGAAGTATTA2300.34612490594431905No Hit
CATAGAAGGACAAAGACATTTAGGACCCTGTCTCCCAAGTATTAGAGCAA2280.3431151241534989No Hit
CATAGACCAGCAGACACTCCCATCATCAAGGAAGTTTCTGTGAGATCTAT2130.32054176072234764No Hit
CATAGAAGGACAAAGACATTTAGGACCCAAGGGGTAAGGCGTACTGGTCT2090.3145221971407073No Hit
CATAGAAAATTTGTGATGGTAAATCATGGGACATGCCAGGAATCAATGTT1870.28141459744168545No Hit
CATAGACCAGCAGACACTCCCATCATTTATTTTAAAGGACTGGAAGCCTC1820.2738901429646351No Hit
CATAGACCTCCGACAGTTTTTATGCACCCACAGCCAGGAAAGAATCTATG1720.2588412340105342No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACTGCTGGGGTTGCTCTATGAG1700.2558314522197141No Hit
CATAGACCTAAAAGGCTATTGAAAGGAAGGCTAATCTGAAATCTTCGTGT1660.24981188863807377No Hit
CATAGATCAAGGAAAACACCAGCCCAATCTCTCATCAAGGAAGTTTCTGT1660.24981188863807377No Hit
CATAGACCAGCAGACACTCCCATCATCAGGAACCATGGGAGGTCTATGAG1650.24830699774266365No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGACGCTATACCACACTA1530.23024830699774268No Hit
CATAGATGTTTTATCAAAGGCATGCTCATGGAGACAGCCGCTCACCTCTA1490.22422874341610236No Hit
CATAGATGTTTTATCAAAGGCATGCTCATACATGGTTCTACACATAGAGG1440.21670428893905191No Hit
CATAGACCAGCAGACACTCCCATCATTGCTTCTTGTGGTAACTGGTCTAT1420.21369450714823174No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACCACACTGACGCTATACCACA1390.2091798344620015No Hit
CATAGAAAAAACAGTTGGCCAGGCATCAAGGGGTAAGGCGTACTGGTCTA1350.20316027088036118No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTGACGCTATACCACACTACA1330.20015048908954103No Hit
CATAGAGATCCCACCATTTGCCATCTATGAGACCAAGTCTCTGCTACCGT1310.19714070729872085No Hit
CATAGAGCTTGGGTGCGAACATCAAGGAAGTTTCTGTGAGATCTATGAGA1300.19563581640331074No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGTTCTGGGCCCCAGTGTGAA1260.18961625282167044No Hit
CATAGAAATGATGCTGAAGGGTCTGTTTCCCCACTCAAATACCATCTATG1250.18811136192626035No Hit
CATAGATGTTTTATCAAAGGCATGCTCATATGGTCTACCAGTTATTAGTT1220.18359668924003011No Hit
CATAGACTCATTGCACTTTCCACAACTGTACACTGACGGTTCTACACATA1190.17908201655379985No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGACGACATGGTTCTACA1140.17155756207674944No Hit
CATAGAAATGATGCTGAAGTGATGGGCTAGATCTATGAGACCAAGTCTCT1090.16403310759969902No Hit
CATAGATCATGGGACTTCAGCATGGCCAACATGGTGAAACCCCATCTCTA1070.16102332580887885No Hit
CATAGAAATGATGCTGAAGGGCCTGCACTGACGACATGGTTCTACACATA980.1474793077501881No Hit
CATAGAAATGATGCTGAAGGGCCTGCTACACATAGAAATGATGCTGAAGT950.14296463506395787No Hit
CATAGACCTCCGACAGTTTTTATGCACTGTCCCAACTGTCTCTGAAAAAT930.1399548532731377No Hit
CATAGACCAGCAGACACTCCCATCATGTCTCAGAGATCCCACCATTTGCC910.13694507148231755No Hit
CATAGATCATGGGACTTCAGCATGGTGGAAGGGTAAGAGAGGGTCTATGA910.13694507148231755No Hit
CATAGATGGCCAAGATACGAGCACATAGATGGCCAAGGGGTAAGGCGTAC890.13393528969149737No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTCTGCAGATTTGGACCTGCG880.13243039879608728No Hit
CATAGAGTAGTTAAAACCCACAATGACAGTGTAGGCTAATCTGAAATCTT870.1309255079006772No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGCTTGCAGATTTGGACCTGCG840.12641083521444696No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTCGCAGATTTGGACCTGCG830.12490594431903689No Hit
CATAGAAATGATGCTGAAGGGCCTGCACATAGAAATGATGCTGAAGTGAT830.12490594431903689No Hit
CATAGAAAAAACAGTTGGCCAGGCATGCTGAAGTGATGGGCTAGATCTAT810.12189616252821671No Hit
CATAGAAATGATGCTGAAGGGCCTGGTTCTACACATAGAAATGATGCTGA800.12039127163280662No Hit
CATAGATCAAGGAAAACACCAGCCCACCCACAGCCAGGAAAGAATCTATG790.11888638073739653No Hit
CATAGATGTGTAGCCTGGGTCTGACAGGTCTACCAGTTATTAGTTGAATG790.11888638073739653No Hit
CATAGAGTCTGAATGGTGGGAGAGTGGTGGAAGGGTAAGAGAGGGTCTAT780.11738148984198646No Hit
CATAGACTCATTGCACTTTCCACAACTGCCCTCTCTGCTGTTCCTTCTAT780.11738148984198646No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTGTGCACCTTCATTGTTGCTCTA740.11136192626034613No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGGTTTGGACCTGCG720.10835214446952596No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGATGCTGAAGTGATGGGCTAGAT690.1038374717832957No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCCGCG690.1038374717832957No Hit
CATAGAGTCTGAATGGTGGGAGAGTGCTGAAGTGATGGGCTAGATCTATG670.10082768999247554No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCTATAGCAGCATAATTTCCCA670.10082768999247554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)