FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83720
Sequences flagged as poor quality0
Sequence length150
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA3107437.116579073100816No Hit
CATAGATTGCACATAAAGTCTGATCCCATCTCAAGTCTCACTCTTCTATG47225.64022933588151No Hit
CATAGAGCCTGGCATTTGAGGTAAGGCTTGTGAGGGCTGAAGTCTATGTG26213.130673674151935No Hit
CATAGACTACCACTACCCAGCACAGGAGCACCCTCTATGTGTAGAACCAT16842.01146679407549No Hit
CATAGAAGCAGCATTGATCATCCTCCACCCCATCCTACATGTGTCTATGT15301.8275203057811753No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGTGATTACAGGTG12941.5456282847587195No Hit
CATAGAGAATACAATGAACTGTGATGGCAGTGTACTCCTATGTAATGTCT12401.4811275680840899No Hit
CATAGACTGTTCTTTTAAATTAACACAGTCAGAGAGGCACTTGGTCCAGA10231.2219302436693742No Hit
CATAGACTACCACTACCCAGCACAGCTACTTCTCTATGCACTTTGCCACA9961.1896798853320594No Hit
CATAGAGATGTCAAGAGCAATGCCCAGCCACCCCATCCTACATGTGTCTA9941.1872909698996654No Hit
CATAGACTACCACTACCCAGTACAGGAGCACCCTCTATGTGTAGAACCAT9461.129956999522217No Hit
CATAGACCATCCTCCCACCTTGACCTCCTGAGTAGCTGAGACCACAGGCA7570.9042044911610129No Hit
CATAGAGAATACAATGAACTGTGATGGCAGTGTAGCATTTTAACACAGCA6240.7453416149068323No Hit
CATAGATGACATAGACCTTATACCCTCATCAGTGTTAGGGATAGTTTGAG6160.7357859531772575No Hit
CATAGACCATCTGCGCATCCTGTCTGACACTCTTGGTCTTTGTGGATCTA6040.7214524605828954No Hit
CATAGACCATCCTCCCACCTTGACCTCTTAAGTAGCTGGGACTACAGGTG5760.6880076445293837No Hit
CATAGATGACATAGACCTTATACCCTCATCAGTGTAGGGATAGTTTGAGG5040.6020066889632106No Hit
CATAGACTACCACCACCCAGTACAGGAGCACCCTCTATGTGTAGAACCAT4950.5912565695174391No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAGGTGTAAGGGAAGCCAC4280.5112279025322504No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGTG3900.4658385093167702No Hit
CATAGACCATCTGCGCATCCTGTCTGTACGGTAGCAGAGGCACTTGGTCC3440.41089345437171526No Hit
CATAGACCATCCTCCCACCTTGACCTGTAGGATGGGTTTTTGCCAGATAG3350.4001433349259436No Hit
CATAGAGATGTCAAGAGCAATGCCCATGCTGAAGTCCCATGATCTATGTG3340.39894887720974676No Hit
CATAGATGACATAGACCTTATACCCTCATCAGTGTAAGGCTTGTGAGGGC3310.3953655040611562No Hit
CATAGAGGGACGGAGGCAAGAGGATAGGGATAGTTTGAGGGTAATGCTCT3300.3941710463449594No Hit
CATAGATCCCAGGCTTTAGAAAATATACCATAGGATGGGTTTTTGCCAGA3270.39058767319636883No Hit
CATAGAGATGTCAAGAGCAATGCCCACCCCATCCTACATGTGTCTATGTG3180.3798375537505972No Hit
CATAGACTGTTCTTTTAAATTAACACAGTCAGAGACCACTCCTATGTAAT3100.37028189202102246No Hit
CATAGAACATCGCACACACATGGAACCATGTCGTCAGTGTAGGGATAGTT2930.34997611084567604No Hit
CATAGACTGTTCTTTTAAATTAACACAGTCAGAGATACGGTAGCATTTTA2840.33922599139990445No Hit
CATAGATGACATAGACCTTATACCCTCATCAACACTGACACTCTTGGTCT2630.3141423793597707No Hit
CATAGACCATCCTCCCACCTTGACCTCCATCTTGACCTCCCAAAGTGCTG2530.30219780219780223No Hit
CATAGACCATCCTCCCACCTTGACCTCCTGAGTAGCTGAGACCACAGGTG2390.28547539417104634No Hit
CATAGAGGTGAGCGGCTGTCTCCAGTGTAGCATTTTAACACAGCATTGAA2340.27950310559006214No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAGAGTGCTGGAATTATAGGCG2180.2603917821309126No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTATTACTACAGGTG2130.2544194935499283No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGCG2080.2484472049689441No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGCA2060.24605828953655043No Hit
CATAGAAGCAGCATTGATCATCCTCCATACGGTAGCATTTTAACACAGCA2000.23889154323936934No Hit
CATAGACTGTTCTTTTAAATTAACACAGTCAGAGACCAAGTAAGGCTTGT1950.2329192546583851No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGAGATTACACACG1930.23053033922599142No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAAGTGAAGGAGAGGCCAC1800.21500238891543239No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGCCA1740.20783564261825133No Hit
CATAGACCATCCTCCCACCTTGACCTCTGGAGTAGCTGGGACTACAGGCC1710.20425226946966077No Hit
CATAGATCCCAGGCTTTAGAAAATATACCATGTCGTCAGTGTACTCCTAT1700.20305781175346393No Hit
CATAGAGATCAATCCCTGTCTACATAACCATGTCGTCAGTGTACTCCTAT1680.2006688963210702No Hit
CATAGATCCCAGGCTTTAGAAAATATACCACCACCCAGTACAGGAGCACC1660.19827998088867652No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGTCCGCGCAGAGCCTTCAGGTCAGA1640.19589106545628282No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG1570.18752986144290493No Hit
CATAGATCCCAGGCTTTAGAAAATATACCACCCAGTACAGGAGCACCCTC1560.18633540372670807No Hit
CATAGACCATCCTCCCACCTTGACCTCATCCTCCCATCTCAAGTCTCACT1470.17558528428093648No Hit
CATAGAAGCAGCATTGATCATCCTCCACCCAGTACAGGAGCACCCTCTAT1400.16722408026755853No Hit
CATAGACTACCACTACCCAGCACAGCCACCCCATCCTACATGTGTCTATG1370.163640707118968No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA1370.163640707118968No Hit
CATAGAAGGTGATAATCATGACAGCCACCCCATCCTACATGTGTCTATGT1340.16005733397037744No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAACAGGTACTGGGATTACAGG1340.16005733397037744No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGAG1330.1588628762541806No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCGGAGCCTTCAGGTCA1290.15408504538939322No Hit
CATAGAGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA1290.15408504538939322No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGGTCCGCGCAGAGCCTTCAGGTCA1250.14930721452460582No Hit
CATAGAGATGTCAAGAGCAATGCCCGGCCACATTATTTTCTATGTGTAGA1240.148112756808409No Hit
CATAGACCATCCTCCCACCTTGACCTATCCTCCCATCTCAAGTCTCACTC1230.14691829909221213No Hit
CATAGAGATGTCAAGAGCAATGCCCAGCCACATTATTTTCTATGTGTAGA1230.14691829909221213No Hit
CATAGACCATCCTCCCACCTTGACCTCTCAAAGTGCTAGGGTTACAGGCA1210.14452938365981843No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA1200.1433349259436216No Hit
CATAGAGATGTCAAGAGCAATGCCCATGCCTAGCCATTTTTGGTTCTATG1200.1433349259436216No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA1180.1409460105112279No Hit
CATAGACCATCCTCCCACCTTGACCTCTCAAGTAGCTGGGATTATGGATG1170.13975155279503107No Hit
CATAGAGGTGAGCGGCTGTCTCCACACGTCCGCGCAGAGCCTTCAGGTCA1150.13736263736263737No Hit
CATAGACTACCACTACCCAGCACAGCATTTTAACACAGCATTGAAGTTCT1140.1361681796464405No Hit
CATAGACATGAGTGGCCGAACAGACCACCAAGAAATAAACCCAAACATTT980.11705685618729096No Hit
CATAGACCATCCTCCCACCTTGACCTTCCAAAGTGCTAGGATAATAGGTG970.11586239847109411No Hit
CATAGACCATCTGCGCATCCTGTCTGAGAAGTGAGGAGTCCCTCTGCCCG950.11347348303870043No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA950.11347348303870043No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAGGCAAAAAAGAAGCAGG940.11227902532250358No Hit
CATAGACCATCCTCCCACCTTGACCTCCTGAGTAGCTGGGACTGCAGGCA930.11108456760630674No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAGTAACTGGGACTACAGGTG910.10869565217391304No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGGGCCTTCAGGTCA890.10630673674151936No Hit
CATAGACCATCCTCCCACCTTGACCTTCCCGGTAGCTGGGACTACAGGCA880.10511227902532251No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)