FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219006
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA7217632.95617471667443No Hit
CATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG106004.840050044291024No Hit
CATAGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA88104.022720838698484No Hit
CATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG81073.7017250668931445No Hit
CATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT62002.8309726674155042No Hit
CATAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC54192.4743614330201No Hit
CATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG44552.0341908440864636No Hit
CATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG40021.8273471959672338No Hit
CATAGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG25951.1848990438618119No Hit
CATAGATGTGAGCACTACTGATAATCCCTGATGTGGCATGGAGAAGGGTC17270.7885628704236415No Hit
CATAGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA16420.7497511483703643No Hit
CATAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT16410.7492945398756198No Hit
CATAGACACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG15190.6935883035167986No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGACACTGGGGGCACAAAGTTCT13580.6200743358629444No Hit
CATAGACTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC11990.547473585198579No Hit
CATAGAGGGGAGACACTGGCTATAAGAACTTGGTCTCATAGAGGGGAGAC11540.5269262029350794No Hit
CATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT11170.5100316886295353No Hit
CATAGATGACCTGTTAATCTGCCCCCAGATCACAGAGTGGCCTTGGGCAA11160.5095750801347909No Hit
CATAGACCAGCTTTGCATGAGTGTGGCAGACCATCTTCTATGTGTAGAAC10390.47441622603946926No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT10240.46756709861830265No Hit
CATAGAGGGGAGACACTGGGGGCACAAAGTTCTATGTGTAGAACCATGTC9400.42921198505977004No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCAAGCAGATCTGTGTTCTATGTGTA8600.3926833054802152No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTGTACGGTAGCAAAGC8530.38948704601700407No Hit
CATAGATTAGGGGTGACGGACTAGCCCTTGGCCTCAGTTTCCCCTCCTTC8240.37624539966941545No Hit
CATAGATTAGGGGTGACGGACTAGCCGACTTGGCCTCAGTTTCCCCTCCT8000.365286795795549No Hit
CATAGACCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG7990.3648301873008045No Hit
CATAGACTTACTGCCCTCACTCCGTCTGCATAGCCACATCTATGTGTAGA7810.3566112343954047No Hit
CATAGATGTGAGCACTACTGATAATCCCTCATTACTCTGGTTCTATGCCA7470.3410865455740939No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTAGACTTGGCCTCAGT7440.33971672008986054No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGAGGGGAGACACTGGGGGCACA7190.32830150772124966No Hit
CATAGAGGGGAGACACTGGCTATAAGAATAGAGGGGAGACACTGGGGGCA7180.3278448992265052No Hit
CATAGACCAGCTTTGCATGAGTGTGGCATGGAGAAGGGTCTATGTGTAGA6780.3095805594367278No Hit
CATAGATGATGTGAAAAGGACCCATGGTCTATGTAGATTTATCCAGCATG6460.2949690876049058No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTCAGAGACTTGGCCTCAGTTTCCC6340.28948978566797257No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGGGAGACACTGGGGGCACAAAG6250.28538030921527263No Hit
CATAGAGGCTGGGTGACTTGGTGAATGAATGTTGGAGAGGCACATCTATG6230.28446709222578376No Hit
CAGTTTCCCCTCCTTCTATGTGTAGAACCATGTCGTCAGTGTAGATCTCG6100.27853118179410613No Hit
CATAGACTTTTCCTCCAATATAGCTGCACTTAATCCCTTTTGCTCATCCA5890.26894240340447295No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCAGAGACTTGGCCTCAGTTTCCCCT5810.26528953544651745No Hit
CATAGATGGTGGGTTCTGGAGAGAGGGCGAAAGTGATCAGAAGCTCTATG5800.264832926951773No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGAGACTTGGTCTCATAGAGGGG5600.25570075705688433No Hit
CATAGACTTACTGCCCTCACTCAGTCTGCATAGCCACATCTATGTGTAGA5230.23880624275134016No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGAGACACTGGGGGCACAAAGTT5210.23789302576185128No Hit
CATAGACCTCCTTTCCTCCACGGCAAAGCTGCTATGGGATCTTCTATGTG4860.22191172844579599No Hit
CATAGATGACCTGTTAATCTGCCCCCACCTCTTCCTTACATGCCGTGTGT4740.21643242650886277No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTACGGTAGCAAAGCTG4640.2118663415614184No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGACTTGGTCTCATAGAGGGGAG4620.21095312457192952No Hit
CATAGATACGATCATAAGGGTGGGGCTGGGCTCAGTGGCTTATGCCTGTA4610.21049651607718511No Hit
CATAGAGTCTCAATTATTCATCTCAACATGCTACCGTAACCAGTGTAGCA4610.21049651607718511No Hit
CATAGACACACCCAACTCTGCTCAGTGTACTCAGTCTGCATAGCCACATC4570.20867008209820734No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTAGCAAAGCTGCTATG4400.20090773768755193No Hit
CATAGAGGTGAGCGGCTGTCTCCATCAATTTAAGGTAGGCACTTCCTCTA4380.19999452069806306No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGGGGAGACACTGGGGGCACAAA4370.19953791220331862No Hit
CATAGACTGGCGGTGATTGCTTTCCTACTTGGCCTCAGTTTCCCCTCCTT4220.1926887847821521No Hit
CATAGACTTACTGCCCTCACTCCGTCTACGGTAGCAAACACTCAAAACAG3730.1703149685396747No Hit
CATAGAGGTGAGCGGCTGTCTCCAGTGTACCTAGTGATTACCTGGGTGAT3680.16803192606595252No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA3510.16026958165529712No Hit
CATAGATGTTTGAAACTCCTCTACCTAAGATAGCAGAGACTTGGCCTCAG3430.15661671369734162No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA3420.1561601052025972No Hit
CATAGAGGTGAGCGGCTGTCTCCAGAAGTCCGCGCAGAGCCTTCAGGTCA3190.14565810982347516No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGGTCCGCGCAGAGCCTTCAGGTCA3140.14337506734975297No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA3110.14200524186551966No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG3000.13698254842333088No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGCCA2970.13561272293909757No Hit
CATAGAGGGGAGACACTGGCTATAAGAATCTCATAGAGGGGAGACACTGG2940.13424289745486423No Hit
CATAGAGTCTCAATTATTCATCTCAACATGCCATGCTGAAGTCCCATGAT2940.13424289745486423No Hit
CATAGACCAGCTTTGCATGAGTGTGGAGAGGCACATCTATGTGTAGAACC2740.12511072755997552No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT2700.12328429358099777No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA2670.12191446809676448No Hit
CATAGACCTGACCTCATGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGG2560.11689177465457566No Hit
CATAGACCAGCTTTGCATGAGTGTGGGGAAGTAGGGAATGATTACAAGTT2520.11506534067559793No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTTCTGGCCTCAGTTTCCCCTCCTT2510.11460873218085349No Hit
CATAGACTTACTGCCCTCACTCCGTCACCCAGGCTGGAGTCCAGTAGCGT2440.11141247271764243No Hit
CATAGATGATGTGAAAAGGACCCATGGTCTCATAGATGATGTGGCATGGA2390.10912943024392026No Hit
CATAGATGACCTGTTAATCTGCCCCCACTCAGTCTGCATAGCCACATCTA2380.10867282174917582No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCGGAGCCTTCAGGTCA2350.10730299626494251No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTTCCCTTGCCCTGTGGCCCTGGCT2290.1045633452964759No Hit
CATAGATTTATCCAGCATGCCCCTCTATGTGTAGAACCATGTCGTCAGTG2210.1009104773385204No Hit
CATAGACACACCCAACTCTGCTCAGTGACTTGGCCTCAGTTTCCCCTCCT2210.1009104773385204No Hit
CATAGACCAGCTTTGCATGAGTGTGGAACACCAGTGTAGCAGACCATCTT2200.10045386884377597No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)