FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005807997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005807997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64992
Sequences flagged as poor quality0
Sequence length150
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA2443237.592319054652876No Hit
CATAGATTGCACATAAAGTCTGATCCCATCTCAAGTCTCACTCTTCTATG40506.231536189069424No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGTGATTACAGGTG30324.6651895617922206No Hit
CATAGACTACCACTACCCAGCACAGGAGCACCCTCTATGTGTAGAACCAT24123.71122599704579No Hit
CATAGAGCCTGGCATTTGAGGTAAGGCTTGTGAGGGCTGAAGTCTATGTG16592.552621861152142No Hit
CATAGAGATGTCAAGAGCAATGCCCAGCCACCCCATCCTACATGTGTCTA16032.466457410142787No Hit
CATAGAAGCAGCATTGATCATCCTCCACCCCATCCTACATGTGTCTATGT14332.204886755292959No Hit
CATAGACTACCACTACCCAGTACAGGAGCACCCTCTATGTGTAGAACCAT9951.5309576563269325No Hit
CATAGAGATGTCAAGAGCAATGCCCGGCCACATTATTTTCTATGTGTAGA7941.2216888232397833No Hit
CATAGACTACCACCACCCAGTACAGGAGCACCCTCTATGTGTAGAACCAT7491.1524495322501231No Hit
CATAGACTGTTCTTTTAAATTAACACAGTCAGAGACTGCTCTTTGACTTA5510.847796651895618No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGTG5270.8108690300344658No Hit
CATAGAGTTTTGTTCCCTTGCTGGCAGAGTTTTGTTCCCTCGCTGACTTC5070.780096011816839No Hit
CATAGAAGCAGCATTGATCATCCTCCACCCAGTACAGGAGCACCCTCTAT4440.6831610044313147No Hit
CATAGAGAATACAATGAACTGTGATGGCAGTGTAAGCTATATAGAAGTAC4430.6816223535204333No Hit
CATAGAGATGTCAAGAGCAATGCCCAGCCACATTATTTTCTATGTGTAGA4130.6354628261939931No Hit
CATAGACTGTTCTTTTAAATTAACACAGTCAGAGACCAAGTACTCCTATG3690.5677621861152141No Hit
CATAGAGAATACAATGAACTGTGATGGCACTTGGTCTCATAGAGAATACA3380.5200640078778926No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAGAGTGCTGGAATTATAGGCG3370.5185253569670113No Hit
CATAGACCATCTGCGCATCCTGTCTGCTCTATGTGTAGAACCATGTCGTC3230.4969842442146726No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTATTACTACAGGTG2930.4508247168882324No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGCG2640.40620384047267355No Hit
CATAGACTACCACTACCCAGCACAGCATTTTAACACAGCATTGAAGTTCT2640.40620384047267355No Hit
CATAGATCCCAGGCTTTAGAAAATATACCACCACCCAGTACAGGAGCACC2630.4046651895617922No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGTGTAAAGTGGGGAAAGGAC2610.4015878877400295No Hit
CATAGAGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA2590.3985105859182669No Hit
CATAGACCATCTGCGCATCCTGTCTGCTGTTTGTCTTCCATGCTTCTATG2590.3985105859182669No Hit
CATAGAGATGTCAAGAGCAATGCCCACCCCATCCTACATGTGTCTATGTG2340.36004431314623336No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAGGTGTAAGGGAAGCCAC2340.36004431314623336No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA2260.34773510585918266No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGGGCTGGGATGACACGCA2200.33850320039389464No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGCA2110.3246553421959626No Hit
CATAGATCCCAGGCTTTAGAAAATATACCACCCAGTACAGGAGCACCCTC2010.3092688330871492No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA1900.29234367306745446No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCGTTAATTACAGGCA1810.2784958148695224No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAACAGGTACTGGGATTACAGG1800.27695716395864106No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGAG1760.27080256031511574No Hit
CATAGAAGCAGCATTGATCATCCTCCATACGGTAGCATTTTAACACAGCA1760.27080256031511574No Hit
CATAGAGAATACAATGAACTGTGATGGCAGTATAAGCTATATAGAAGTAC1660.25541605120630234No Hit
CATAGACTCAAGAAACTTACAATCATGGCATTGCTCTTTGACTTATGATG1540.23695224027572626No Hit
CATAGAACATCGCACACACATGGAACATCGCTGACTTCTCTTAAACAGTT1430.2200270802560315No Hit
CATAGAGATGTCAAGAGCAATGCCCATGCTGAAGTCCCATGATCTATGTG1370.21079517479074347No Hit
CATAGAAGGTGATAATCATGACAGCCACCCCATCCTACATGTGTCTATGT1360.20925652387986213No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTAGGATTACAAGTG1320.20310192023633677No Hit
CATAGACCATCCTCCCACCTTGACCTTCCAAAGTGCTAGGATAATAGGTG1250.1923313638601674No Hit
CATAGATGACATAGACCTTATACCCTCATCACCCAGTACAGGAGCACCCT1210.18617676021664206No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGAGATTACACACG1170.18002215657311668No Hit
CATAGACCATCTGCGCATCCTGTCTGGCTTCCCTGCTTGTCTTTCATCTA1140.17540620384047267No Hit
CATAGACCATCCTCCCACCTTGACCTCTTAAAGTGCTGCGATTACAAGCA1100.16925160019694732No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG1090.16771294928606598No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGCCA1080.16617429837518463No Hit
CATAGACTGTTCTTTTAAATTAACACAGTCAGAGATGGAGCATTTTAACA1060.16309699655342194No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGTCCGCGCAGAGCCTTCAGGTCAGA1050.16155834564254062No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA1040.16001969473165928No Hit
CATAGACTCAAGAAACTTACAATCATGGCATACGGTAGCAGAGGCACTTG1020.15694239290989662No Hit
CATAGAGATCAATCCCTGTCTACATAACCAATAGTTGTTACTACCCCCAC1020.15694239290989662No Hit
CATAGAGCCTGGCATTTGAGGTAAGGCACTTGGTCCAGACTTTCTATGTG1010.15540374199901524No Hit
CATAGACCATCCTCCCACCTTGACCTCTCAAAGTGCTAGGGTTACAGGCA950.14617183653372723No Hit
CATAGATCCCAGGCTTTAGAAAATATACCATGTCGTCAGTGTACTCCTAT940.14463318562284588No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTTGGATTACAGGTG910.14001723289020188No Hit
CATAGACTACCACTACCCAGCACAGGAGCCACCCCATCCTACATGTGTCT910.14001723289020188No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGGTCCGCGCAGAGCCTTCAGGTCA900.13847858197932053No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA860.13232397833579518No Hit
CATAGACCATCCTCCCACCTTGACCTCCATCTTGACCTCCCAAAGTGCTG850.13078532742491383No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGAGATTACAGAAG840.1292466765140325No Hit
CATAGACCATCCTCCCACCTTGACCTCTTGGTCTCATAGACTACCACCAC820.1261693746922698No Hit
CATAGAGGTGAGCGGCTGTCTCCACCCAGTACAGGAGCACCCTCTATGTG810.12463072378138848No Hit
CATAGAAGCAGCATTGATCATCCTCCACCTTTCCCCATCTCCAACCCTCT770.11847612013786313No Hit
CATAGACTCAAGAAACTTACAATCATGGCAGAAAGTGAAGGAGAGGCCAC750.11539881831610045No Hit
CATAGAGGTGAGCGGCTGTCTCCATGTCGTCAGTGTGCCATGCTGAAGTC750.11539881831610045No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTATAGGCA730.11232151649433776No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGGATTACAGGTA730.11232151649433776No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAATTTCTGGGATTACAGGGG730.11232151649433776No Hit
CATAGAAGGTGATAATCATGACAGCCAACTGATCTTTGACAAACCAAACA720.11078286558345643No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGGGCCTTCAGGTCA720.11078286558345643No Hit
CATAGAAGGTGATAATCATGACAGCCAAGTCACCCGACCATCCAAGCCAG710.10924421467257509No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTTCTAGGATTACAGACA710.10924421467257509No Hit
CATAGAGATGTCAAGAGCAATGCCCATGCCTAGCCATTTTTGGTTCTATG680.10462826193993106No Hit
CATAGACCATCCTCCCACCTTGACCTCCCAAAGTGCTGGTATTACAGGTG650.10001230920728704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)