FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005808007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005808007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97170
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNTAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA50875.235154883194402No Hit
NNNNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA50735.220747144180303No Hit
NATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA50325.1785530513532985No Hit
NNNAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA50035.148708449109807No Hit
NNTNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA39214.035196048163013No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA15081.551919316661521No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA14581.5004631058968816No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA13781.4181331686734588No Hit
NNNNGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA13621.4016671812287744No Hit
NNNAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA13171.355356591540599No Hit
NNNAGNGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA12731.3100751260677164No Hit
NNTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA12471.283317896470104No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA12381.2740557785324689No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA11611.1948132139549243No Hit
NATAGAGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA8710.8963671915200165No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT5550.5711639394874961No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT5510.567047442626325No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG4690.48265925697231654No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT4680.48163013275702377No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT4500.46310589688175363No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT4430.4559020273747041No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT4150.4270865493465061No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG4120.42399917670062776No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG4110.4229700524853349No Hit
NNNAGNATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3980.4095914376865287No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT3920.40341669239477207No Hit
NNNNGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3880.39930019553360097No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT3850.3962128228877226No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG3830.39415457445713703No Hit
NNNAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3820.3931254502418442No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT3540.3643099722136462No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT3540.3643099722136462No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG3350.3447566121230833No Hit
NNTAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3310.3406401152619121No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2840.29227127714315115No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2730.28095091077493056No Hit
NNNAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2380.24493156323968304No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2320.2387568179479263No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2320.2387568179479263No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2200.22640732736441288No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2150.22126170628794894No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2110.21714520942677779No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2080.21405783678089943No Hit
NNTAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2080.21405783678089943No Hit
NNTAGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA2030.2089122157044355No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1910.1965627251209221No Hit
NNNAGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1900.19553360090562932No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA1870.19244622825975094No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1850.19038797982916536No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1810.18627148296799423No Hit
NATAGAATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA1770.1821549861068231No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1730.17803848924565197No Hit
NNNNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA1700.1749511165997736No Hit
NNTNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA1670.17186374395389525No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA1660.17083461973860245No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1620.16671812287743132No Hit
NATAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1600.16465987444684574No Hit
NNNNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1590.16363075023155293No Hit
NATAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA1560.16054337758567458No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1540.15848512915508903No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCACC1490.1533395080786251No Hit
NNNAGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1460.15025213543274674No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1450.14922301121745393No Hit
NNNNGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1420.14613563857157558No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1350.1389317690645261No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1320.13584439641864773No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCCTGCTAT1300.13378614798806215No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCACC1290.13275702377276938No Hit
NNTAGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT1290.13275702377276938No Hit
NNTAGNCTTCCTTGCCCCCATTTATTGGATGGGATCTATGTGTAGAACCA1290.13275702377276938No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCCTGCTAT1290.13275702377276938No Hit
NNTNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1280.1317278995574766No Hit
NNNAGNATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1280.1317278995574766No Hit
NNNAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1230.12658227848101267No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCACC1220.12555315426571986No Hit
NNNAGACTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG1220.12555315426571986No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCCTGCTAT1210.12452403005042709No Hit
NNTAGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1190.12246578161984152No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCACC1190.12246578161984152No Hit
NNTAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1180.12143665740454873No Hit
NNTAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1160.11937840897396315No Hit
NNNNGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1160.11937840897396315No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1150.11834928475867038No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCCTGCTAT1140.11732016054337759No Hit
NNNAGNCTGCCTCAGNCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1140.11732016054337759No Hit
NNNAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1130.1162910363280848No Hit
NNNNGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1090.11217453946691365No Hit
NATAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1090.11217453946691365No Hit
NNTAGACTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG1030.10599979417515694No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCAACATGGTG1030.10599979417515694No Hit
NNNAGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT1030.10599979417515694No Hit
NNNNGACTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG1020.10497066995986415No Hit
NNNAGNCTGCCTCAGNCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG1000.10291242152927857No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCCTGCTAT990.1018832973139858No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCAACATGGTG980.10085417309869302No Hit
NNNAGNCTTCCTTGCCCCCATTTATTGGATGGGATCTATGTGTAGAACCA980.10085417309869302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)