FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005808049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005808049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13158
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA146111.103511171910625No Hit
CATAGACCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAAGG144410.974312205502356No Hit
CATAGATTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC141810.77671378628971No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA8106.155950752393981No Hit
CATAGATATGCCCATGGTTAGGAAGGAAGGATCAATATTGTTAAAATGGG5754.369965040279677No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG4753.6099711202310383No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCACGCCAC3052.3179814561483507No Hit
CATAGACCTCCTGTTTGACCATTTTGACTGTTATTACAAAATAATGTTAA2171.6491868065055477No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGAATTACAGGTGCCCGCCAC1941.4743882048943608No Hit
CATAGACTGTCATTGCTTGCCCATTGGGATTTAAAAAAAGGAAAAGAACA1881.4287885696914424No Hit
CATAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1651.2539899680802553No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACGACAGCCCGGCTACTG1441.094391244870041No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1361.03359173126615No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGACTGCAGGCGCATGCTTG1341.018391852865177No Hit
CATAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT1310.9955920352637179No Hit
CATAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA1280.9727922176622587No Hit
CATAGATGGAGAGAGTGAGGGAAAGGAAATTATGGGAGCCCTGTGAAACG1160.8815929472564219No Hit
CATAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA1160.8815929472564219No Hit
CATAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1140.8663930688554491No Hit
CATAGATGCATCAGCAACTCACAGATTATGACATAAAATATGTGATAAAT1110.84359325125399No Hit
CATAGAATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT1100.8359933120535035No Hit
CATAGAAGCAGGATGAAAACATGGTACATCCTACATCAGCAAGATGTAAA1090.8283933728530172No Hit
CATAGACTGTCATTGCTTGCCCATTGCTTGTTTTTCTCAGGTTTGTCAAA1050.7979936160510716No Hit
CATAGACATGCTAACATTTCTGGGGATAGAGACTTGGTCTCATAGACATG970.7371941024471804No Hit
CATAGATTGAGCTTAAATGATCTGTGCAACCCCTTAAGAAAATTTAATTG940.7143942848457212No Hit
CATAGAAGTTCTGATCCACATTCACGTATGTGTATAAATTATTTTGGTGC740.5623955008359933No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT690.5243958048335613No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG670.5091959264325885No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCCCGCCAG630.478796169630643No Hit
CATAGAATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG620.47119623043015657No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTGTCAAATTTGTTAAAAGAGG560.4255965952272382No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGAGATTATAGGTACTAATTTT540.4103967168262654No Hit
CATAGAAAGGCCTTCCTGTATCAGTCAGTGTCAACCCCTTAAGAAAATTT500.37999696002431976No Hit
CATAGACTGCCTCAGCCTCCTGAGTAACTGGAACCACAGGCATGTGCCAC470.3571971424228606No Hit
CATAGACTGCCTCAGCCTCCTGAGTAACTGGGATTACAGGCGCCCGCCAC430.32679738562091504No Hit
CATAGACATGCTAACATTTCTGGGGATAGATTGGTCTCATAGACATGCTA430.32679738562091504No Hit
CATAGACCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG420.31919744642042863No Hit
CATAGACCCACTTTCGTGACTTTGTGTAGAACCATGTCGTCAGTGTTCGT420.31919744642042863No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACATGCCAC410.3115975072199422No Hit
CATAGACATGCTAACATTTCTGGGGATAGTTCAGAAAATTAGAGTACATT390.29639762881896947No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGAGATTACAGATGTGCACCAT390.29639762881896947No Hit
CATAGACATGCTAACATTTCTGGGGATTGTGACTCTGTTTTCTATGTGTA380.288797689618483No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGTACCACAGGCATGTGCCAC370.28119775041799666No Hit
CATAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG350.26599787201702385No Hit
CATAGAAAGGCCTTCCTGTATCAGTCAGTGTTGGAAGTACACAAAAAGGG340.2583979328165375No Hit
CATAGACATGCTAACATTTCTGGGGATAGACATGCTAACATTACTGGGGA330.25079799361605104No Hit
CATAGAAAGAGACAGAAGAGAAGGTCATTTAAGCTCAATCTATGAGACGA320.24319805441556466No Hit
CATAGACCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAGGG270.2051983584131327No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAAACAAACAAACAAAAATGTTCCA250.18999848001215988No Hit
CATAGAGTTCAAATGGCCAATCAGGTAGCAGAAATATTTCTAATTGGGCA240.1823985408116735No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGAATTACAGGCATGAGCCAC240.1823985408116735No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCCTGCTAC240.1823985408116735No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTGAAAGATCA230.17479860161118713No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTAATACAAAAAATTAG230.17479860161118713No Hit
CATAGATGAAAAGTCAAGCCAGCACAGTGAAACCCCATCTCTACTAAAAA200.15199878400972794No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGAGAATATAGGCGTGCACCAC200.15199878400972794No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTGTCAGGTTTGTCAAAGATGA190.1443988448092415No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTAGTGAAATTCCTGAGGTCAGG180.13679890560875513No Hit
CATAGAAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT180.13679890560875513No Hit
CATAGACCAGACTTTTGAAAGAAGCACATCACTTATACGCCTCCCTGTCT180.13679890560875513No Hit
CATAGATGGAGAGAGTGAGGGAAAGGGGGTGCTGTTGTAAACCCCATCTC170.12919896640826875No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCGTGCCAC150.11399908800729594No Hit
CATAGACTGCCTCAGCCTCCTGAGTAGCTGCGATTACAGGCGCCTGCCAC150.11399908800729594No Hit
CATAGACTGCCTCAGCCTCCTGAGTATGTGTAGAACCATGTCGTCAGTGT140.10639914880680955No Hit
CATAGAATCCTTTCCCCATTGCTTGTCCATTGGGATTTAAAAAAAGGAAA140.10639914880680955No Hit
CATAGAATCCTTTCCCCATTGCTTGTTTTTGTCAGGTTTGTCAAAGATCA140.10639914880680955No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)