FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005808141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005808141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134999
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA50263.7229905406706716No Hit
NNTAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA50063.7081756161156747No Hit
NNNNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA48313.578545026259454No Hit
NATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA46883.4726183156912276No Hit
NNTNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA38362.8415025296483676No Hit
NNNNGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA13450.9963036763235283No Hit
NNNAGNGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA12740.9437106941532901No Hit
NNNAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA12290.9103771139045475No Hit
NNTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA11880.8800065185668042No Hit
NNNAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG11740.8696360713783066No Hit
NNTAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG11100.822228312802317No Hit
NNNNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG10410.7711168230875785No Hit
NATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG8800.651856680419855No Hit
NNTAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG8460.6266713086763606No Hit
NNTNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG8450.6259305624486108No Hit
NNNAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG8120.6014859369328661No Hit
NATAGAGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA7700.5703745953673731No Hit
NNNNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG7570.5607448944066252No Hit
NATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG6350.47037385462114534No Hit
NNTNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG6330.46889236216564567No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA6090.45111445269964956No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA6070.44963296024415No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA6020.4459292291054007No Hit
NNNAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT5740.42518833472840534No Hit
NNTAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT5640.41778087245090706No Hit
NATAGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA5230.3874102771131638No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA4520.3348172949429255No Hit
NNNNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT4490.332595056259676No Hit
NATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT4410.32666908643767734No Hit
NNTNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT4340.3214838628434285No Hit
NNNAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG4200.3111134156549308No Hit
NNTAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG4120.3051874458329321No Hit
NNTAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG4090.30296520714968256No Hit
NNNAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG4030.29852072978318356No Hit
NNNAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA3780.28000207408943767No Hit
NNTAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA3740.27703908917843834No Hit
NNNNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3680.27259461181193934No Hit
NNNNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA3640.26963162690094No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3380.2503722249794443No Hit
NATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3360.24889073252394464No Hit
NNNNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3350.24814998629619478No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3290.24370550892969578No Hit
NATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3210.23777953910769709No Hit
NNNNGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3090.22889058437469906No Hit
NNTNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3080.22814983814694925No Hit
NNTNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA3060.22666834569144956No Hit
NATAGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA2970.22000162964170106No Hit
NNTNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG2920.21629789850295186No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC2810.2081496899977037No Hit
NNNAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2790.20666819754220403No Hit
NNNAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2740.20296446640345484No Hit
NNTAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2700.2000014814924555No Hit
NNNAGNGTCTCAATTATTCATCTCAACATGCTACACTGATGTGGCATGGA2640.1955570041259565No Hit
NNNAGNCCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2570.19037178053170764No Hit
NNTAGNGTCTCAATTATTCATCTCAACATGCTACACTGATGTGGCATGGA2530.1874087956207083No Hit
NATAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC2440.1807420795709598No Hit
NNNNGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2420.1792605871154601No Hit
NNTAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2300.17037163238246208No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC2280.16889013992696242No Hit
NATAGAGTCTCAATTATTCATCTCAACATGCTACACTGATGTGGCATGGA2180.16148267764946406No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2150.15926043896621458No Hit
NNNNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2150.15926043896621458No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2120.15703820028296506No Hit
NNNNGNGTCTCAATTATTCATCTCAACATGCTACACTGATGTGGCATGGA2110.15629745405521522No Hit
NNNAGNTACGATCATNAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2010.14888999177771686No Hit
NNNAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1960.1451862606389677No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1940.14370476818346803No Hit
NATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1910.14148252950021853No Hit
NNTNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1870.13851954458921917No Hit
NNTAGNGTCTCAATTATTCATCTCAACATGCTTACGGTAGCAAAGCTGCT1860.13777879836146936No Hit
NNTNGNGTCTCAATTATTCATCTCAACATGCTACACTGATGTGGCATGGA1860.13777879836146936No Hit
NNNAGNGGGGAGACACTGGCTATAAGAAATACCTGGGCGACCTCTCTATG1830.13555655967821983No Hit
NNTAGNGTTATTCAGAGAGAGGGGAAATGAGAGAAAACTACCCCAGGCTC1800.13333432099497033No Hit
NNNAGNGTCTCAATTATTCATCTCAACATGCTTACGGTAGCAAAGCTGCT1760.13037133608397097No Hit
NNNAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1700.125926858717472No Hit
NNTAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1670.12370462003422247No Hit
NNNNGNGGGGAGACACTGGCTATAAGAAATACCTGGGCGACCTCTCTATG1650.12222312757872279No Hit
NNTAGNGGGGAGACACTGGCTATAAGAAATACCTGGGCGACCTCTCTATG1620.12000088889547329No Hit
NNNAGNTGTGAGCACTACTGATAATCCCTTTTGCTCATCCATTGTTGATC1600.11851939643997363No Hit
NATAGAGTCTCAATTATTCATCTCAACATGCTGAGAGAAAACTACCCCAG1580.11703790398447395No Hit
NNNNGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA1560.11555641152897428No Hit
NNNAGNGTCTCAATTATTCATCTCAACATGCTGAGAGAAAACTACCCCAG1540.11407491907347463No Hit
NATAGAGGGGAGACACTGGCTATAAGAAATACCTGGGCGACCTCTCTATG1540.11407491907347463No Hit
NNTAGNTGTGAGCACTACTGATAATCCCTTTTGCTCATCCATTGTTGATC1540.11407491907347463No Hit
NNNAGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA1530.11333417284572478No Hit
NATAGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1520.11259342661797496No Hit
NNNAGNCCAGCTTTGNATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA1480.10963044170697561No Hit
NNNNGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1470.10888969547922576No Hit
NNTNGNGTCTCAATTATTCATCTCAACATGCTGAGAGAAAACTACCCCAG1430.10592671056822645No Hit
NNNNGNGTTATTCAGAGAGAGGGGAAATGAGAGAAAACTACCCCAGGCTC1430.10592671056822645No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1430.10592671056822645No Hit
NATAGACCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG1410.10444521811272676No Hit
NATAGATGTGAGCACTACTGATAATCCCTTTTGCTCATCCATTGTTGATC1400.10370447188497692No Hit
NNNNGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1390.1029637256572271No Hit
NNTAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1390.1029637256572271No Hit
NNNAGNCACCATTGGNGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1380.10222297942947725No Hit
NNTAGNTGTTTGAAACTCCTCTACCTAAGAAATACCTGGGCGACCTCTCT1380.10222297942947725No Hit
NNNNGNTGTGAGCACTACTGATAATCCCTTTTGCTCATCCATTGTTGATC1360.1007414869739776No Hit
NNNAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1360.1007414869739776No Hit
NNTAGNGTCTCAATTATTCATCTCAACATGCTGAGAGAAAACTACCCCAG1360.1007414869739776No Hit
NNNNGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1350.10000074074622775No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)