FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005808177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005808177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82868
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA11511.3889559299126322No Hit
NNTAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA11171.3479268233817638No Hit
NNNNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA11101.3394796543901144No Hit
NNTAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG10811.3044842399961385No Hit
NATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA10731.2948303325771107No Hit
NNNAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG10011.2079451658058598No Hit
NNNNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG9001.0860645846406332No Hit
NNTNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA8901.0739972003668485No Hit
NNTNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG8040.9702176956122991No Hit
NATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG7840.9460829270647295No Hit
NNNAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG7770.9376357580730801No Hit
NNTAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG7660.9243616353719168No Hit
NNNNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG6800.8205821306173674No Hit
NNTAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT6220.7505913018294155No Hit
NNTNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG6000.7240430564270889No Hit
NNNAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT5960.719216102717575No Hit
NATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG5790.6987015494521407No Hit
NNNNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT5360.6468117970748661No Hit
NNNAGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT5080.6130231211082686No Hit
NATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT5030.6069894289713762No Hit
NNTAGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT4650.5611333687309938No Hit
NNTNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT4590.5538929381667229No Hit
NNNNGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT4510.5442390307476951No Hit
NATAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT4500.5430322923203166No Hit
NNNAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT4200.5068301394989622No Hit
NNTAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG4160.5020031857894484No Hit
NNTAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG4090.4935560167977989No Hit
NNNAGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT4070.49114253994304197No Hit
NNNAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3910.47183472510498625No Hit
NNTAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT3750.45252691026693054No Hit
NNNAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3660.4416662644205242No Hit
NNTAGNCACACCCAACTCTGCTCAGTTCTTTTTGTTTGGCAGACACACAC3640.43925278756576724No Hit
NNTAGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT3630.43804604913838874No Hit
NNNNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3540.4271854032919824No Hit
NNNAGNCACACCCAACTCTGCTCAGTTCTTTTTGTTTGGCAGACACACAC3530.42597866486460395No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3530.42597866486460395No Hit
NNTNGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT3500.4223584495824685No Hit
NNNNGNCACACCCAACTCTGCTCAGTTCTTTTTGTTTGGCAGACACACAC3490.42115171115509004No Hit
NNNNGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT3440.4151180190181976No Hit
NNNNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3360.40546411159916973No Hit
NATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3250.39218998889800644No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3180.3837428199063571No Hit
NNNNGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT3180.3837428199063571No Hit
NATAGACACACCCAACTCTGCTCAGTTCTTTTTGTTTGGCAGACACACAC3160.38132934305160016No Hit
NATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3120.37650238934208624No Hit
NNTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA3010.3632282666409229No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3000.36202152821354444No Hit
NNTNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG2970.358401312931409No Hit
NATAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT2950.355987836076652No Hit
NNTNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG2950.355987836076652No Hit
NNNAGNGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA2900.3499541439397596No Hit
NNNNGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2880.34754066708500264No Hit
NNNAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA2800.3378867596659748No Hit
NATAGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT2790.3366800212385963No Hit
NNTNGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT2790.3366800212385963No Hit
NNTNGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT2710.3270261138195685No Hit
NATAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC2650.3197856832552976No Hit
NNTNGNCACACCCAACTCTGCTCAGTTCTTTTTGTTTGGCAGACACACAC2590.31254525269102673No Hit
NNNNGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA2580.3113385142636482No Hit
NNNAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2580.3113385142636482No Hit
NNNAGNCCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2520.30409808369937735No Hit
NNTAGNGGCTGGGTGACTTGGTGAATCTCAGTACAACTGACAAGGGCACA2500.3016846068446204No Hit
NNNAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2430.293237437852971No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC2430.293237437852971No Hit
NNTAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2410.290823960998214No Hit
NNNAGNTACGATCATNAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2260.2727228845875368No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2060.2485881160399672No Hit
NNTAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2060.2485881160399672No Hit
NNNNGNGGCTGGGTGACTTGGTGAATCTCAGTACAACTGACAAGGGCACA2050.24738137761258872No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1980.23893420862093934No Hit
NNNNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1970.23772747019356086No Hit
NATAGAGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA1950.2353139933388039No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1930.23290051648404692No Hit
NNNNGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1920.23169377805666844No Hit
NNNAGNGGCTGGGTGACTTGGTGAATCTCAGTACAACTGACAAGGGCACA1920.23169377805666844No Hit
NNTNGNGGCTGGGTGACTTGGTGAATCTCAGTACAACTGACAAGGGCACA1910.23048703962928996No Hit
NNTAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1880.22686682434715452No Hit
NATAGAGGCTGGGTGACTTGGTGAATCTCAGTACAACTGACAAGGGCACA1840.22203987063764058No Hit
NNNAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1820.21962639378288362No Hit
NNTAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1720.2075590095090988No Hit
NNNAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1690.20393879422696334No Hit
NATAGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1610.19428488680793551No Hit
NNTNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1590.19187140995317856No Hit
NATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1550.18704445624366461No Hit
NNNNGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1530.18463097938890766No Hit
NNNAGNCACCATTGGNGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1520.18342424096152918No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1430.17256359511512284No Hit
NNNNGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1430.17256359511512284No Hit
NATAGACCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG1390.1677366414056089No Hit
NNTAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1320.15928947241395955No Hit
NNNAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1270.15325578027706716No Hit
NNNAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1240.1496355649949317No Hit
NNTAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1220.14722208814017473No Hit
NNTAGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1180.14239513443066082No Hit
NNNAGNGATGATGGGATCTTATATTTGGAAAAACTTAACAACTCCACAAA1160.13998165757590383No Hit
NNNNGACACACCCAACTCTGCTCAGTTCTTTTTGTTTGGCAGACACACAC1140.13756818072114688No Hit
NNTAGNGATGATGGGATCTTATATTTGGAAAAACTATTAGCGAGACTCTA1130.1363614422937684No Hit
NNNAGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1130.1363614422937684No Hit
NATAGACACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1130.1363614422937684No Hit
NNNAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1100.13274122701163296No Hit
NNNNGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1090.13153448858425448No Hit
NNTAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1090.13153448858425448No Hit
NNTAGNGATGATGGGATCTTATATTTGGAAAAACTTAACAACTCCACAAA1050.12670753487474054No Hit
NNNNGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1050.12670753487474054No Hit
NNNAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1050.12670753487474054No Hit
NNNAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG1040.1255007964473621No Hit
NNNAGNGATGATGGGATCTTATATTTGGAAAAACTATTAGCGAGACTCTA1030.1242940580199836No Hit
NNTNGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1020.12308731959260512No Hit
NNNNGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC1010.12188058116522664No Hit
NNNAGNCCTGACCTCNTGATCTGCCTGTGTTCTTTGCATGATCTATGTGT990.11946710431046967No Hit
NNNAGNGTTTGCCCTNCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT990.11946710431046967No Hit
NNNNGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT990.11946710431046967No Hit
NATAGATACGATCATNAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG980.11826036588309119No Hit
NNTNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA980.11826036588309119No Hit
NNNNGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG980.11826036588309119No Hit
NNNNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA970.11705362745571271No Hit
NNNNGNGATGATGGGATCTTATATTTGGAAAAACTTAACAACTCCACAAA960.11584688902833422No Hit
NNNNGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT960.11584688902833422No Hit
NNTAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG950.11464015060095574No Hit
NNNAGNCCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG940.11343341217357726No Hit
NNNAGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT930.11222667374619878No Hit
NNTAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG920.11101993531882029No Hit
NATAGAGATGATGGGATCTTATATTTGGAAAAACTTAACAACTCCACAAA910.10981319689144181No Hit
NNNNGNGATGATGGGATCTTATATTTGGAAAAACTATTAGCGAGACTCTA910.10981319689144181No Hit
NATAGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA900.10860645846406333No Hit
NNNAGNTGATGTGAAAAGGACCCATGGTCTATGTGTAGCCATGCTGAAGT890.10739972003668485No Hit
NNNAGACACACCCAACTCTGCTCAGTTCTTTTTGTTTGGCAGACACACAC890.10739972003668485No Hit
NNNAGNCTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC890.10739972003668485No Hit
NNTAGNCTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC880.10619298160930636No Hit
NNNAGNGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT870.10498624318192788No Hit
NNNNGNGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT870.10498624318192788No Hit
NNNAGNCTTTTCCTCCAATATAGCTGCACTGACTTGGCCTCAGTTTCCCC860.1037795047545494No Hit
NNNAGNCTGGCGGTGNTTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT860.1037795047545494No Hit
NATAGAGATGATGGGATCTTATATTTGGAAAAACTATTAGCGAGACTCTA850.10257276632717093No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA850.10257276632717093No Hit
NNNAGNCACACCCAANTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG850.10257276632717093No Hit
NNTAGNTGATGTGAAAAGGACCCATGGTCTATGTGTAGCCATGCTGAAGT850.10257276632717093No Hit
NATAGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA850.10257276632717093No Hit
NNNAGNCACACCCAANTCTGCTCAGTTCTTTTTGTTTGGCAGACACACAC840.10136602789979243No Hit
NNTNGNGATGATGGGATCTTATATTTGGAAAAACTATTAGCGAGACTCTA840.10136602789979243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)