FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005808179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005808179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65430
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNTAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA23423.579397829741709No Hit
NNNNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA21853.339446736970809No Hit
NNNAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA21813.3333333333333335No Hit
NATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA21583.2981812624178515No Hit
NNTNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA17402.6593305823016964No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA6591.0071832492740334No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA6520.9964847929084518No Hit
NNNNGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA5730.8757450710683172No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA5700.871160018340211No Hit
NNNAGNGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA5670.8665749656121045No Hit
NNNAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA5580.8528198074277854No Hit
NNTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA5440.8314228946966224No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA5190.7932141219624026No Hit
NNTAGNCCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAAGG4690.716796576493963No Hit
NNNAGNCCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAAGG4660.7122115237658566No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA4580.6999847164909063No Hit
NNNNGNCCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAAGG4030.6159254164756228No Hit
NATAGAGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA3700.5654898364664527No Hit
NATAGACCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAAGG3690.5639614855570839No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT3540.541036221916552No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT3400.5196393091853889No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT3260.4982423964542259No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT3230.49365734372611947No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT3060.4676753782668501No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT3060.4676753782668501No Hit
NNTNGNCCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAAGG3050.4661470273574813No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT3050.4661470273574813No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT3040.4646186764481125No Hit
NNNAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2810.4294666055326303No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2630.40195628916399206No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT2630.40195628916399206No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2590.39584288552651686No Hit
NNNNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2490.380559376432829No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2420.36986092006724747No Hit
NNTAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2390.3652758673391411No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2270.3469356564267156No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT2260.3454073055173468No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2220.33929390187987163No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2110.32248204187681495No Hit
NNTAGNTGACTTGGGGGAAAATGGTAGAAACAGCCAGTTTTCCCTTCTAT2110.32248204187681495No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2050.3133119364206022No Hit
NNTNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2030.31025523460186455No Hit
NNTAGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA2030.31025523460186455No Hit
NATAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2020.3087268836924958No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA1910.2919150236894391No Hit
NNNNGNTGACTTGGGGGAAAATGGTAGAAACAGCCAGTTTTCCCTTCTAT1880.2873299709613327No Hit
NATAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1880.2873299709613327No Hit
NNNAGNTGACTTGGGGGAAAATGGTAGAAACAGCCAGTTTTCCCTTCTAT1830.27968821641448877No Hit
NNNNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1810.2766315145957512No Hit
NNNAGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1790.2735748127770136No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT1760.26898976004890723No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA1730.26440470732080085No Hit
NNNAGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1710.2613480055020633No Hit
NNTAGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1690.2582913036833257No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1640.25064954913648174No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG1610.24606449640837535No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1610.24606449640837535No Hit
NATAGATGACTTGGGGGAAAATGGTAGAAACAGCCAGTTTTCCCTTCTAT1600.24453614549900657No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1570.2399510927709002No Hit
NNTAGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG1520.23230933822405625No Hit
NNTAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA1490.22772428549594986No Hit
NNNNGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA1490.22772428549594986No Hit
NNNAGNATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA1460.22313923276784353No Hit
NNTNGNTGACTTGGGGGAAAATGGTAGAAACAGCCAGTTTTCCCTTCTAT1450.2216108818584747No Hit
NNNAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA1410.21549747822099957No Hit
NNTNGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1410.21549747822099957No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG1400.21396912731163073No Hit
NNNNGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1370.20938407458352437No Hit
NATAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1350.20632737276478677No Hit
NNTNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1340.20479902185541798No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG1320.20174232003668044No Hit
NNNNGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG1320.20174232003668044No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG1320.20174232003668044No Hit
NNNAGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG1250.19104386367109888No Hit
NNTAGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA1220.18645881094299252No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1220.18645881094299252No Hit
NNTAGNATAGCCAGCAGAAGGAAGCAACCCAAATGGACATTTCACTTATA1210.18493046003362373No Hit
NNNAGNATAGCCAGCAGAAGGAAGCAACCCAAATGGACATTTCACTTATA1180.18034540730551735No Hit
NNTAGACCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAAGG1170.17881705639614856No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG1170.17881705639614856No Hit
NNTNGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG1150.17576035457741096No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCACC1140.17423200366804217No Hit
NNNAGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA1130.1727036527586734No Hit
NATAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG1090.16659024912119821No Hit
NNTNGNATAGCCAGCAGAAGGAAGCAACCCAAATGGACATTTCACTTATA1090.16659024912119821No Hit
NNNNGACCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAAGG1080.16506189821182946No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCACC1060.16200519639309185No Hit
NNNNGNAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA1050.16047684548372307No Hit
NNTAGNAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA1000.1528350909368791No Hit
NNNNGNATAGCCAGCAGAAGGAAGCAACCCAAATGGACATTTCACTTATA990.15130674002751032No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG960.14672168729940394No Hit
NNNAGNCCCTGGATTNGCATGAGGAACATGGCTTCCCATTTCAGACAAGG930.14213663457129758No Hit
NNNAGACCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAAGG930.14213663457129758No Hit
NNNAGNCTGCCTCAGNCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT920.1406082836619288No Hit
NNNNGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT920.1406082836619288No Hit
NATAGAATAGCCAGCAGAAGGAAGCAACCCAAATGGACATTTCACTTATA910.13907993275255998No Hit
NNTAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT900.1375515818431912No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT890.1360232309338224No Hit
NNNNGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA890.1360232309338224No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT880.13449488002445362No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT880.13449488002445362No Hit
NNNAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT870.13296652911508483No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCACC860.13143817820571602No Hit
NNNAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT840.12838147638697844No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCACC830.12685312547760966No Hit
NNTNGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA830.12685312547760966No Hit
NNNAGNAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA810.12379642365887208No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCACC810.12379642365887208No Hit
NNNAGNATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT790.1207397218401345No Hit
NATAGAATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA780.1192113709307657No Hit
NNNNGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT770.11768302002139691No Hit
NNNAGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT770.11768302002139691No Hit
NNNNGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA760.11615466911202812No Hit
NATAGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA750.11462631820265932No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT740.11309796729329054No Hit
NATAGAAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA730.11156961638392177No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC730.11156961638392177No Hit
NNNNGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT720.11004126547455295No Hit
NNNNGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT710.10851291456518417No Hit
NNNAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT710.10851291456518417No Hit
NNTAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT690.10545621274644658No Hit
NNNNGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA690.10545621274644658No Hit
NNNAGNCCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG670.10239951092770899No Hit
NATAGAATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT670.10239951092770899No Hit
NNTAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA670.10239951092770899No Hit
NATAGACCCTGGATTNGCATGAGGAACATGGCTTCCCATTTCAGACAAGG670.10239951092770899No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC670.10239951092770899No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)