FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005808224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005808224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113460
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG3848433.91856160761502No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCGTGGAGGAAAGGAGGTCTATGAG46164.068394147717258No Hit
CATAGAGCTTCTGATCACTTTCGCCCCACCCTTATGATCGTATCTATGAG34543.0442446677243082No Hit
CATAGAACCAGAGTAATGAGGGATGCTCAGGTCTCCAATGGTGTCTATGA31302.7586814736471004No Hit
CATAGACTCAAGCAATCCTCCCACCTCAGCCTCCCAAGTAGCCAGGACTA27622.4343380927199014No Hit
CNTAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG25402.2386744227040367No Hit
CATAGACTCAAGCAATCCTCCCACCTCAGCCTCCCAAATAGCCAGGACTA24372.147893530759739No Hit
CATAGAACTTTGTGCCCCCAGTGTCTTAGGTAGAGGAGTTTCAAACATCT23712.0897232504847523No Hit
CATAGAACCAGAGTAATGAGGGATGCTCTCTCCAGAACCCACCATCTATG17901.577648510488278No Hit
CATAGATCATGCAAAGAACACAGGCCACACTCATGCAAAGCTGGTCTATG15331.3511369645690112No Hit
CATAGATGTGGCTATGCAGACTGAGCAGAGTTGGGTGTGTCTATGAGACC14721.297373523708796No Hit
CATAGAACTTGTAATCATTCCCTACTTCCCACACTCATGCAAAGCTGGTC12861.1334390974792878No Hit
CATAGATGTGCCTCTCCAACATTCATTTCCCCTCTCTCTGAATAACTCTA12641.1140490040542923No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCAGATTTGGACCTGCGAGCGGGTTC7730.6812973735237088No Hit
CATAGATCATGCAAAGAACACAGGCAGATCATGAGGTCAGGTCTATGAGA5730.5050237969328398No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCGTGGAGGAAAGCAATCACCGCCA5140.45302309183853345No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATGAGACCAAGTCTCTG5070.44685351665785294No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCGTGGAGGGAAGGAAGGGCAAACT4870.42922615899876604No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCTCTGCTACCGTAGGCTAGTC4480.3948528115635466No Hit
CATAGATCACCTACACAGACAGCTTTCCATGATTCACCAAGTCACCCAGC4220.37193724660673366No Hit
CATAGAACACAGATCTGCTTGGAGGTAGCATAGAGTAACCTCCTCCAGTC4210.3710558787237793No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTTCTTATAGCCAGTGTCTCC4080.3595980962453728No Hit
CATAGATGTGGCTATGCAGACTGAGTAGAGTTGGGTGTGTCTATGAGACC4050.3569539925965098No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTTCTTATAGCCAGTGTCT4040.35607262471355544No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCTCTGGGAGGGAAGGAAGGGC4040.35607262471355544No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCCATGTCTTCTGTGTTTGGTCTATG3950.34814031376696636No Hit
CATAGAACTTGTAATCATTCCCTACTTCCCTTTCAGCATCTCTTTGGCTC3730.32875022034197077No Hit
CATAGAAGAATTGCTTGAATCCGGGAGGGAAGGAAGGGCAAACTCTATGA3140.2767495152476644No Hit
CATAGAGGGGCATGCTGGATAAATCTTAGGTAGAGGAGTTTCAAACATCT3070.270579940066984No Hit
CATAGAACTTGTAATCATTCCCTACTTCCCATAGAAAACCACTTGAACAT3010.2652917327692579No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTCTCAGTTTTTCCAAATATAAG2980.26264762912039485No Hit
CATAGAGCTTCTGATCACTTTCGCCCTCTCTCCAGAACCCACCATCTATG2950.2600035254715318No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATGAGACCAAGTCTTCT2350.20712145249427114No Hit
CATAGAAGATCCCATAGCAGCTTTGCCGTGGAGGAAAGGAGGTCTATGAG2240.1974264057817733No Hit
CNTAGAGGGCTGTTTTGAGTGTTTGCCGTGGAGGAAAGGAGGTCTATGAG2230.196545037898819No Hit
CATAGAAGAATTGCTTGAATCCGGGAGGCAGAGGTTGCAGTGAGCTGAGA2230.196545037898819No Hit
CATAGATGTGGCTATGCAGACGGAGTGAGGGCAGTAAGTCTATGAGACCA2220.19566367001586463No Hit
CATAGATCAACAATGGATGAGCAAAAGGAAAGCAATCACCGCCAGTCTAT2210.19478230213291028No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCTTCTTATAGCCAGTGTCTCCC2180.19213819848404723No Hit
CATAGACTCAAGCAATCCTCCCACCTCAGCTTCCCTAAGTACCAGGATTA2120.18684999118632117No Hit
CATAGAGCCTGGGGTAGTTTTCTCTCACACTGACGACATGGTTCTACACA2020.17803631235677772No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTAGGAAAGCAATCACCGCCAGT2010.17715494447382338No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTCTGCAGATTTGGACCTGCG1960.17274810505905164No Hit
CNTAGAGCTTCTGATCACTTTCGCCCCACCCTTATGATCGTATCTATGAG1950.1718667371760973No Hit
CATAGAAGGAGGGGAAACTGAGACCAAGTCTCTGCTACCGTAAAGCGTAG1920.16922263352723427No Hit
CATAGATGTGGCTATGCAGACTGAGTGAGGGCAGTAAGTCTATGAGACCA1910.16834126564427992No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGGCCTGCG1880.1656971619954169No Hit
CNTAGACTCAAGCAATCCTCCCACCTCAGCCTCCCAAGTAGCCAGGACTA1860.16393442622950818No Hit
CATAGATGTGGCTATGCAGACTGAGTGCAGCTATATTGGAGGAAAAGTCT1860.16393442622950818No Hit
CATAGAACACAGATCTGCTTGGAGGGAAGGAAGGGCAAACTCTATGAGAC1840.1621716904635995No Hit
CATAGACCCTTCTCCATGCCACACTCATGCAAAGCTGGTCTATGAGACCA1830.16129032258064516No Hit
CATAGAACTTTGTGCCCCCAGTGTCTTCTTATAGCCAGTGTCTCCCCTCT1800.15864621893178213No Hit
CATAGAAGGAGGGGAAACTGAGGCCACACTCATGCAAAGCTGGTCTATGA1800.15864621893178213No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGCTTTGGACCTGCG1720.15159527586814736No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTCGCAGATTTGGACCTGCG1690.14895117221928433No Hit
CNTAGAACCAGAGTAATGAGGGATGCTCAGGTCTCCAATGGTGTCTATGA1640.1445443328045126No Hit
CATAGAAGAATTGCTTGAATCCGGGAGGAAAGCAATCACCGCCAGTCTAT1560.13749338974087785No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGTGGAGGGAAGGAAGGGCAAACT1530.1348492860920148No Hit
CATAGAGGGCTGTTTTGAGTGTTTGCTACCGTAGACGGAGTGAGGGCAGT1520.13396791820906045No Hit
CNTAGACTCAAGCAATCCTCCCACCTCAGCCTCCCAAATAGCCAGGACTA1490.13132381456019743No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCGGATTTGGACCTGCG1490.13132381456019743No Hit
CATAGATCATGGGACTTCAGCATGGCTAGTCCGTCACCCCTAATCTATGA1480.13044244667724308No Hit
CATAGAGCTTCTGATCACTTTCGCCCTAGAAAACCACTTGAACATTGCCT1470.12956107879428874No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCTCTCCAGAACCCACCATCTATGA1460.1286797109113344No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCCGCG1460.1286797109113344No Hit
CATAGAAGAATTGCTTGAATCCGGGAGGCAGAGGTTGCAGTGAGCCAAGA1440.12691697514542571No Hit
CATAGATCAACAATGGATGAGCAAAAGGCAGATCATGAGGTCAGGTCTAT1410.12427287149656266No Hit
CATAGAACTTTGTGCCCCCAGTGTCTCCCCTCTATGAGACCAAGTTCTTA1350.11898466419883659No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCCGATTTGGACCTGCG1340.11810329631588225No Hit
CATAGATCATGGGACTTCAGCATGGCATGTTGAGATGAATAATTGAGACT1330.1172219284329279No Hit
CATAGATGTGCCTCTCCAACATTCATGAGGCAGATCATGAGGTCAGGTCT1320.11634056054997356No Hit
CATAGAAGGAGGGGAAACTGAGGCCAAGGAGGGAAGGAAGGGCAAACTCT1310.11545919266701922No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTCGGACCTGCG1300.11457782478406486No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGCCCTGCG1260.1110523532522475No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGACTTGGACCTGCG1230.10840824960338445No Hit
CNTAGAACTTTGTGCCCCCAGTGTCTTAGGTAGAGGAGTTTCAAACATCT1200.10576414595452141No Hit
CATAGATGTGGCTATGCAGACTGAGTGGGGGCAGATTAACAGGTCATCTA1200.10576414595452141No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)