FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005808237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005808237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28646
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNTAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA3341.1659568526146757No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT3231.1275570760315576No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT3211.1205752984709907No Hit
NNNAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA3071.0717028555470223No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT3051.0647210779864553No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2981.0402848565244711No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2850.9949033023807862No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2770.9669761921385184No Hit
NNNNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2680.9355581931159673No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2600.9076310828736996No Hit
NNTAGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG2520.8797039726314319No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2430.8482859736088808No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2430.8482859736088808No Hit
NATAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2400.8378133072680304No Hit
NNNNGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT2330.8133770858060462No Hit
NNTAGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA2320.8098861970257628No Hit
NNTAGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA2310.8063953082454793No Hit
NNNAGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA2310.8063953082454793No Hit
NNTNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2190.7645046428820778No Hit
NNNNGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG2170.7575228653215108No Hit
NNTAGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT2170.7575228653215108No Hit
NNNAGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA2170.7575228653215108No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2160.7540319765412273No Hit
NNNAGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG2140.7470501989806605No Hit
NATAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG2060.7191230887383928No Hit
NNNAGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT2030.7086504223975425No Hit
NATAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1970.6877050897158417No Hit
NNNNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1960.6842142009355582No Hit
NNTNGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG1870.6527962019130071No Hit
NNNNGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA1870.6527962019130071No Hit
NATAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1820.6353417580115898No Hit
NNTNGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA1770.6178873141101725No Hit
NATAGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA1740.6074146477693221No Hit
NNTNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1660.5794875375270544No Hit
NNTNGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1540.5375968721636528No Hit
NNNAGNTTGAGCTTAAATGATCTGTGCAACCCCTTAAGAAAATTTAATTG1220.4258884311945822No Hit
NNTAGNTTGAGCTTAAATGATCTGTGCAACCCCTTAAGAAAATTTAATTG1210.42239754241429867No Hit
NNNNGNTTGAGCTTAAATGATCTGTGCAACCCCTTAAGAAAATTTAATTG1200.4189066536340152No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT1110.3874886546114641No Hit
NNTAGNTGGAGAGAGTGAGGGAAAGGACCAACTCAGATGGGATCCTCTAT1110.3874886546114641No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT1080.37701598827061367No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT1060.3700342107100468No Hit
NNTNGNTTGAGCTTAAATGATCTGTGCAACCCCTTAAGAAAATTTAATTG1050.3665433219297633No Hit
NNNAGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT1050.3665433219297633No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT1030.3595615443691964No Hit
NNNNGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT960.3351253229072122No Hit
NATAGATTGAGCTTAAATGATCTGTGCAACCCCTTAAGAAAATTTAATTG960.3351253229072122No Hit
NNNAGNTGGAGAGAGTGAGGGAAAGGACCAACTCAGATGGGATCCTCTAT960.3351253229072122No Hit
NNTAGNGGTGAGCGGCTGTCTCCAACCCCTTAAGAAAATTTAATTGTCTA910.3176708790057949No Hit
NNTAGNGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA890.310689101445228No Hit
NNNAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA880.30719821266494446No Hit
NNNAGNTTGCATAGCNATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT880.30719821266494446No Hit
NATAGAATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT850.2967255463240941No Hit
NNTAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA840.2932346575438107No Hit
NNNNGNGGTGAGCGGCTGTCTCCAACCCCTTAAGAAAATTTAATTGTCTA830.2897437687635272No Hit
NNNAGNGGTGAGCGGCTGTCTCCAACCCCTTAAGAAAATTTAATTGTCTA820.2862528799832437No Hit
NNNNGNTGGAGAGAGTGAGGGAAAGGACCAACTCAGATGGGATCCTCTAT820.2862528799832437No Hit
NNNAGNTGCATCAGCAACTCACAGATTACACTGGGGACTGTGACTCTGTT820.2862528799832437No Hit
NNTAGNTGCATCAGCAACTCACAGATTACACTGGGGACTGTGACTCTGTT800.2792711024226768No Hit
NATAGAAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT800.2792711024226768No Hit
NNTAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT790.27578021364239336No Hit
NNTAGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT780.2722893248621099No Hit
NATAGATGGAGAGAGTGAGGGAAAGGACCAACTCAGATGGGATCCTCTAT780.2722893248621099No Hit
NNNAGNTTGCATAGCNATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA770.2687984360818264No Hit
NNNNGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA770.2687984360818264No Hit
NNNNGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA760.265307547301543No Hit
NNNNGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA760.265307547301543No Hit
NNNNGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT750.2618166585212595No Hit
NNTNGNGGTGAGCGGCTGTCTCCAACCCCTTAAGAAAATTTAATTGTCTA710.24785310340012567No Hit
NNNAGNTTGCATAGCNATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA700.24436221461984223No Hit
NNNAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG700.24436221461984223No Hit
NATAGAGGTGAGCGGCTGTCTCCAACCCCTTAAGAAAATTTAATTGTCTA680.23738043705927528No Hit
NNNAGNAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA670.23388954827899183No Hit
NNNNGNTGCATCAGCAACTCACAGATTACACTGGGGACTGTGACTCTGTT670.23388954827899183No Hit
NATAGAGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA660.23039865949870836No Hit
NNNAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA660.23039865949870836No Hit
NNNAGNCCAGACTTTNGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG650.2269077707184249No Hit
NNNNGNGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA650.2269077707184249No Hit
NNTAGNGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT650.2269077707184249No Hit
NNTNGNTGCATCAGCAACTCACAGATTACACTGGGGACTGTGACTCTGTT640.22341688193814147No Hit
NNNNGNAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA640.22341688193814147No Hit
NNNAGNGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA640.22341688193814147No Hit
NNTNGNGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT640.22341688193814147No Hit
NNNAGNGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT630.21992599315785796No Hit
NNTNGNTGGAGAGAGTGAGGGAAAGGACCAACTCAGATGGGATCCTCTAT620.21643510437757452No Hit
NATAGATGCATCAGCAACTCACAGATTACACTGGGGACTGTGACTCTGTT620.21643510437757452No Hit
NNNAGNATCCTTTCCNCATTGCTTGCTCATTATTATTGGATGGGATCTAT620.21643510437757452No Hit
NNNAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA610.2129442155972911No Hit
NNNAGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA610.2129442155972911No Hit
NNTAGNAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA600.2094533268170076No Hit
NNNNGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG590.20596243803672415No Hit
NNTNGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT590.20596243803672415No Hit
NNNAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT580.2024715492564407No Hit
NNNAGNCCTCCTGTTTGACCATTTTGAATGAGAGTCCCTGTTGCTCTATG550.1919988829155903No Hit
NNTAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA550.1919988829155903No Hit
NNNNGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA540.18850799413530683No Hit
NNTNGNGGTGAGCGGCTGTCTCCATGCTGAAGTCCCATGATCTATGTGTA540.18850799413530683No Hit
NNTAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA530.1850171053550234No Hit
NATAGAGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT530.1850171053550234No Hit
NNNNGNATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG530.1850171053550234No Hit
NNNNGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT530.1850171053550234No Hit
NNNAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT520.18152621657473994No Hit
NNNAGNGGTGAGCGGNTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA520.18152621657473994No Hit
NNNNGNATCCTTTCCCCATTGCTTGTGACTTCGTCTCAGCCCAAAATCTC510.17803532779445647No Hit
NNTAGNCCTCCTGTTTGACCATTTTGAATGAGAGTCCCTGTTGCTCTATG500.174544439014173No Hit
NATAGAATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG490.17105355023388955No Hit
NNTAGNATCCTTTCCCCATTGCTTGTGACTTCGTCTCAGCCCAAAATCTC490.17105355023388955No Hit
NATAGACCAGACTTTNGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG490.17105355023388955No Hit
NNNNGNGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT480.1675626614536061No Hit
NNTAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT480.1675626614536061No Hit
NNTAGNATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG480.1675626614536061No Hit
NNNAGNATCCTTTCCCCATTGCTTGTGACTTCGTCTCAGCCCAAAATCTC470.16407177267332262No Hit
NNNNGNCCTCCTGTTTGACCATTTTGAATGAGAGTCCCTGTTGCTCTATG470.16407177267332262No Hit
NATAGAAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA470.16407177267332262No Hit
NNTAGNTTTAAAATAAAATTCCTGGATAGATCAGTGTACTGGGGACTGTG450.1570899951127557No Hit
NNTAGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA440.15359910633247223No Hit
NATAGACCTCCTGTTTGACCATTTTGAATGAGAGTCCCTGTTGCTCTATG440.15359910633247223No Hit
NNTNGNATCCTTTCCCCATTGCTTGTGACTTCGTCTCAGCCCAAAATCTC430.1501082175521888No Hit
NNNAGNATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG420.14661732877190534No Hit
NNNAGNCTGTCATTGNTTGCCCATTGCTTGCTCATTATTATTGGATGGGA410.14312643999162186No Hit
NNNNGAATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT400.1396355512113384No Hit
NNTNGNTTTAAAATAAAATTCCTGGATAGATCAGTGTACTGGGGACTGTG400.1396355512113384No Hit
NNTAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG390.13614466243105494No Hit
NATAGATTGCATAGCNATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA390.13614466243105494No Hit
NATAGATTTAAAATAAAATTCCTGGATAGATCAGTGTACTGGGGACTGTG380.1326537736507715No Hit
NNTAGATTGAGCTTAAATGATCTGTGCAACCCCTTAAGAAAATTTAATTG380.1326537736507715No Hit
NNNNGNTTTAAAATAAAATTCCTGGATAGATCAGTGTACTGGGGACTGTG370.12916288487048802No Hit
NATAGATTGCATAGCNATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT360.12567199609020457No Hit
NNNAGNCATGCTAACATTTCTGGGGATAGCAGAGACTTGGTCTCATAGAC360.12567199609020457No Hit
NNTNGNAAGGCCTTCCTGTATCAGTCATCACTTATACGCCTCCCTGTCTA360.12567199609020457No Hit
NNNAGNTTTAAAATAAAATTCCTGGATAGATCAGTGTACTGGGGACTGTG360.12567199609020457No Hit
NNTNGNCCTCCTGTTTGACCATTTTGAATGAGAGTCCCTGTTGCTCTATG350.12218110730992111No Hit
NATAGACTGTCATTGCTTGCCCATTGCTCATTATTATTGGATGGGATCTA350.12218110730992111No Hit
NNTNGNCATGCTAACATTTCTGGGGATAGCAGAGACTTGGTCTCATAGAC340.11869021852963764No Hit
NATAGACTGTCATTGNTTGCCCATTGCTTGCTCATTATTATTGGATGGGA330.11519932974935418No Hit
NNNAGNTGGAGAGAGNGAGGGAAAGGACCAACTCAGATGGGATCCTCTAT330.11519932974935418No Hit
NNTAGNCCTCCTGTTTGACCATTTTGAACATAGCCTTTGGGGTTTCTATG330.11519932974935418No Hit
NNTNGNAAGGCCTTCCTGTATCAGTCAGTGTCATCACTTATACGCCTCCC330.11519932974935418No Hit
NNTAGNTGGAGAGAGTGAGGGAAAGGATTCTATGAGACCAGCCATGCTGA330.11519932974935418No Hit
NNTAGNTTTAAAATAAAATTCCTGGATAGATCAGTGTATGGAAGTACACA320.11170844096907073No Hit
NNTNGNATAGCCAGCAGAAGGAAGCAGGAATTAGACTCTTGACATCTATG320.11170844096907073No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCTAAAGCCCTTGTGAAGTTCTATG320.11170844096907073No Hit
NNNAGAATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT320.11170844096907073No Hit
NNTAGNCATGCTAACATTTCTGGGGATAGCAGAGACTTGGTCTCATAGAC320.11170844096907073No Hit
NNNAGNCATGCTAACATTTCTGGGGATATGCTAACATTACTGGGGACTGT310.10821755218878726No Hit
NNTAGNCCATCCAGAGGTAATTCTGCTCATTATTATTGGATGGGATCTAT310.10821755218878726No Hit
NNTNGNCCATCCAGAGGTAATTCTGCTCATTATTATTGGATGGGATCTAT310.10821755218878726No Hit
NNTAGNGTTCAAATGGCCAATCAGGTTGTCTGGGTGGTTTCTGTCTATGT310.10821755218878726No Hit
NNTAGNTTGCATAGCCATGTGTAGCCAGATGATATGAACCCAAAGGTCTA310.10821755218878726No Hit
NNNAGNTTGCATAGCCATGTGTAGCCAGATGATATGAACCCAAAGGTCTA310.10821755218878726No Hit
NNNNGNCCATCCAGAGGTAATTCTGCTCATTATTATTGGATGGGATCTAT300.1047266634085038No Hit
NNTAGNCTGTCATTGCTTGCCCATTGCTCATTATTATTGGATGGGATCTA300.1047266634085038No Hit
NATAGACATGCTAACATTTCTGGGGATAGCAGAGACTTGGTCTCATAGAC300.1047266634085038No Hit
NNNAGNTGGAGAGAGTGAGGGAAAGGATTCTATGAGACCAGCCATGCTGA300.1047266634085038No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTACGGGAGCAGGAATTAGACTCTT300.1047266634085038No Hit
NNNAGNTTGAGCTTANATGATCTGTGCAACCCCTTAAGAAAATTTAATTG300.1047266634085038No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTATGTGTAGAACCATGTCGTCAGTGT290.10123577462822035No Hit
NNNNGNAAGGCCTTCCTGTATCAGTCAGTGTCATCACTTATACGCCTCCC290.10123577462822035No Hit
NNNNGNTGGAGAGAGTGAGGGAAAGGATTCTATGAGACCAGCCATGCTGA290.10123577462822035No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)