FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005808297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005808297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73129
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNTAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG11421.5616239795429994No Hit
NNNAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG11151.5247029222333137No Hit
NNNNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG10281.4057350709021044No Hit
NATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG9341.2771950936017176No Hit
NNTAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG9081.2416414828590574No Hit
NNNAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG8461.1568597957034827No Hit
NNNNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG8061.1021619330224672No Hit
NNTNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG7641.0447291772074006No Hit
NATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG7050.9640498297529024No Hit
NNTNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG7040.9626823831858771No Hit
NNTAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT6970.9531102572166992No Hit
NNNAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT6810.9312311121442929No Hit
NNTAGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT6020.8232028333492869No Hit
NNNNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT5790.7917515623077027No Hit
NNNAGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT5760.7876492226066267No Hit
NNNNGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT5600.7657700775342203No Hit
NATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT5330.7288490202245347No Hit
NATAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT5230.7151745545542808No Hit
NNTNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT4990.6823558369456714No Hit
NNTNGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT4580.6262905276976303No Hit
NNTAGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT4500.615350955161427No Hit
NNNAGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT4200.5743275581506653No Hit
NNTAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT4200.5743275581506653No Hit
NNNAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT4130.5647554321814875No Hit
NNNNGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT3930.5374065008409796No Hit
NNTAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3830.5237320351707258No Hit
NATAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT3670.5018528900983193No Hit
NNNNGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT3660.500485443531294No Hit
NNNNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3480.4758714053248369No Hit
NNNAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3430.46903417248970997No Hit
NNTNGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT3390.4635643862216084No Hit
NATAGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT3380.46219693965458303No Hit
NNNNGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3140.4293782220459736No Hit
NNNAGNCCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3080.42117354264382123No Hit
NATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG2960.40476418383951646No Hit
NNTNGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT2940.4020292907054657No Hit
NNNAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2890.39519205787033873No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC2880.39382461130331337No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC2880.39382461130331337No Hit
NNNAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG2860.3910897181692625No Hit
NATAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC2750.3760478059319832No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC2680.36647567996280545No Hit
NNTNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG2670.3651082333957801No Hit
NNTAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG2660.36374078682875466No Hit
NNTAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2500.34186164175634837No Hit
NNTAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2430.33228951578717064No Hit
NATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG2420.3309220692201452No Hit
NNNNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG2360.32271738981799286No Hit
NNNNGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2340.3199824966839421No Hit
NNNAGNTACGATCATNAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2320.3172476035498913No Hit
NNNNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2300.3145127104158405No Hit
NNTNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG2270.31041037071476435No Hit
NNNAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2160.295368458477485No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC2150.2940010119104596No Hit
NNTAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2130.2912661187764088No Hit
NNNAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2090.2857963325083072No Hit
NATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1940.26528463400292634No Hit
NNNNGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1770.24203804236349463No Hit
NNNAGNCACCATTGGNGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1690.23109846982729151No Hit
NNTNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1660.22699613012621533No Hit
NNNAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1630.22289379042513915No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1600.21879145072406297No Hit
NNNAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1600.21879145072406297No Hit
NNTAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1580.21605655759001216No Hit
NNNAGNGTTTGCCCTNCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1540.21058677132191062No Hit
NNNAGNCCTGACCTCATGATCTGCCTTCAGAACGCGGCAAATGGTCTATG1520.2078518781878598No Hit
NNNNGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1510.20648443162083444No Hit
NNNAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1510.20648443162083444No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1500.20511698505380904No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1500.20511698505380904No Hit
NATAGACCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG1470.20101464535273286No Hit
NATAGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1440.19691230565165668No Hit
NNTAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1430.19554485908463126No Hit
NNTAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1420.19417741251760587No Hit
NNNNGNCCTGACCTCATGATCTGCCTTCAGAACGCGGCAAATGGTCTATG1390.19007507281652972No Hit
NNTAGNCCTGACCTCATGATCTGCCTTCAGAACGCGGCAAATGGTCTATG1360.1859727331154535No Hit
NATAGACCTGACCTCATGATCTGCCTTCAGAACGCGGCAAATGGTCTATG1220.166828481177098No Hit
NNNNGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT1200.16409358804304722No Hit
NNTNGNCCTGACCTCATGATCTGCCTTCAGAACGCGGCAAATGGTCTATG1190.16272614147602182No Hit
NNTAGNTGATGTGAAAAGGACCCATGGCCTCAGTTTCCCCTCCTTCTATG1180.16135869490899643No Hit
NNNAGNCTGGCGGTGNTTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT1140.15588890864089486No Hit
NNTAGNCCTGACCTCATGATCTGCCTCTTGGCCTCAGTTTCCCCTCCTTC1130.15452146207386946No Hit
NNTAGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT1120.15315401550684407No Hit
NNNNGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1120.15315401550684407No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1110.15178656893981868No Hit
NATAGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1110.15178656893981868No Hit
NNTAGNCCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG1110.15178656893981868No Hit
NNNNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1110.15178656893981868No Hit
NNTAGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1090.1490516758057679No Hit
NNNAGNTGATGTGAAAAGGACCCATGGCCTCAGTTTCCCCTCCTTCTATG1070.1463167826717171No Hit
NNTNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1040.14221444297064093No Hit
NNNNGNTGATGTGAAAAGGACCCATGGCCTCAGTTTCCCCTCCTTCTATG1040.14221444297064093No Hit
NATAGACACCATTGGNGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1030.14084699640361553No Hit
NNNNGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG1020.13947954983659014No Hit
NNNAGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1010.13811210326956475No Hit
NNNAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1010.13811210326956475No Hit
NNNAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG1000.13674465670253935No Hit
NNNAGNCCTGACCTCNTGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1000.13674465670253935No Hit
NATAGATACGATCATNAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG990.13537721013551396No Hit
NNTAGNTGTTTGAAACTCCTCTACCTAAGACTTGGCCTCAGTTTCCCCTC990.13537721013551396No Hit
NNNAGNCTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC960.13127487043443778No Hit
NNTAGNCTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC940.128539977300387No Hit
NNNAGNCCTGACCTCATGATCTGCCTCTTGGCCTCAGTTTCCCCTCCTTC920.1258050841663362No Hit
NNTAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT910.12443763759931081No Hit
NNTAGNGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT900.1230701910322854No Hit
NNTAGNCCAAACACAGAAGACATGGCAAAGCTGCTATGGGATCTTCTATG900.1230701910322854No Hit
NNTAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG900.1230701910322854No Hit
NATAGATGTTTGAAACTCCTCTACCTAAGACTTGGCCTCAGTTTCCCCTC880.12033529789823463No Hit
NATAGACACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG880.12033529789823463No Hit
NNNNGNCCAAACACAGAAGACATGGCAAAGCTGCTATGGGATCTTCTATG870.11896785133120923No Hit
NNNAGNTGTTTGAAACTCCTCTACCTAAGACTTGGCCTCAGTTTCCCCTC860.11760040476418385No Hit
NNNAGNCCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG850.11623295819715845No Hit
NNNAGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT850.11623295819715845No Hit
NNNAGNTGTGAGCACTACTGATAATCCCTCATTACTACCAGTGTAGCAGA840.11486551163013305No Hit
NNNAGNTGGTGGGTTNTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG840.11486551163013305No Hit
NNNAGNCACACCCAANTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG840.11486551163013305No Hit
NNNNGNGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT840.11486551163013305No Hit
NNNNGNCCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG830.11349806506310767No Hit
NNNAGNGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT830.11349806506310767No Hit
NNTNGNTGATGTGAAAAGGACCCATGGCCTCAGTTTCCCCTCCTTCTATG830.11349806506310767No Hit
NNTNGNGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT820.11213061849608226No Hit
NNNAGNGTCTCAATTATTCATCTCAACATGCTGATGTTTTAAAATGAGCA810.11076317192905687No Hit
NNTNGNCCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG800.10939572536203149No Hit
NNNAGNCCAAACACAGAAGACATGGCAAAGCTGCTATGGGATCTTCTATG790.10802827879500608No Hit
NNNNGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG790.10802827879500608No Hit
NNNNGNGTTTGCCCTTCCTTCCCTCCTACGGTAGCAAAGCTGCTATGGGA790.10802827879500608No Hit
NNNNGNGTCTCAATTATTCATCTCAACATGCTGATGTTTTAAAATGAGCA790.10802827879500608No Hit
NATAGATGATGTGAAAAGGACCCATGGCCTCAGTTTCCCCTCCTTCTATG790.10802827879500608No Hit
NNTAGNTGTGAGCACTACTGATAATCCCTCATTACTACCAGTGTAGCAGA780.10666083222798069No Hit
NNTNGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG770.10529338566095531No Hit
NNTAGNGTCTCAATTATTCATCTCAACATGCTGATGTTTTAAAATGAGCA770.10529338566095531No Hit
NNTAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC760.1039259390939299No Hit
NNTNGNCCAAACACAGAAGACATGGCAAAGCTGCTATGGGATCTTCTATG760.1039259390939299No Hit
NNNNGNTGTTTGAAACTCCTCTACCTAAGACTTGGCCTCAGTTTCCCCTC750.10255849252690452No Hit
NNNNGNCTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC740.10119104595987911No Hit
NNNNGNCCTGACCTCATGATCTGCCTCTTGGCCTCAGTTTCCCCTCCTTC740.10119104595987911No Hit
NATAGACCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG740.10119104595987911No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)