FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005808300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005808300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144599
Sequences flagged as poor quality0
Sequence length150
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGCTTGGGTGCGAACATCAAGGAAGTTTCTGTGAGATCTATGAGA1595011.030505051902157No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGTAATAACCAGGCCCCTCTATGAG84835.866568925096301No Hit
CATAGACGGATTATTCTCTCCATGGTCTACCAGTTATTAGTTGAATGAGC79215.4779078693490275No Hit
CATAGAGAAGACTCCCTCAACCCACCCACAGCCAGGAAAGAATCTATGAG71204.923962129751935No Hit
CATAGATCATGGGACTTCAGCATGGCGGTGTTTGCAGATTTGGACCTGCG46373.2067994937724325No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGTTCTGGGCCCCAGTGTGAA43843.0318328619146744No Hit
CATAGAAATGATGCTGAAGGGCCTGCCCTCTCTGCTGTTCCTTCTATGAG42242.9211820275382263No Hit
CATAGACTCATTGCACTTTCCACAACTGTGTGCTTGAACCAGATTCTATG39262.715094848512092No Hit
CATAGATCATGGGACTTCAGCATGGCTCAGGTATGTCTTTATTAGCTCTA38192.6410971030228425No Hit
CATAGAAATGATGCTGAAGGGCCTGTTTCCCCACTCAAATACCATCTATG33552.3202096833311434No Hit
CATAGACTCATTGCACTTTCCACAACTGTCATTGTCGCTGTCCCATCTAT31782.197802197802198No Hit
CATAGATTGTGTGTCTCTTCTATATTAACAGTCCCAACTGTCTCTGAAAA30962.1410936451842684No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAGGAACCATGGGAGGTCTAT26301.8188230900628635No Hit
CATAGACCAGCAGACACTCCCATCATTTATTTTAAAGGACTGGAAGCCTC25161.7399843705696445No Hit
CATAGATGTTTTATCAAAGGCATGCTCATCAAGGAAGTTTCTGTGAGATC24961.7261530162725882No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTCTTCCTTTTCTGCCTTCATCTA24771.713013229690385No Hit
CATAGATCACGTAGAAACTGGAGAGGCTAATCTGAAATCTTCGTGTTCTA23661.6362492133417241No Hit
CATAGACTCATTGCACTTTCCACAACTGTCCCAACTGTCTCTGAAAAATT22231.537355030117774No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGAAGTGATGGGCTAGATCTATGA18001.2448218867350396No Hit
CATAGAAGCTGAGAAGACTTCCTAATGCAGAAGTAGGAAGTATTACCCCT16571.1459277035110893No Hit
CATAGAGGAGGTGAAAGAAATCTACAATGGGCCCCAGTGTGAATACTTCT15631.080920338314926No Hit
CATAGATCAAGGAAAACACCAGCCCACGCTGTCCTTCTCTGAATCTATGA13530.9356911181958382No Hit
CATAGATGTGTAGCCTGGGTCTGACATGCCAGGAATCAATGTTCTATGAG11590.8015269815143949No Hit
CATAGAGAAGACTCCCTCAACCCACGCTGTCCTTCTCTGAATCTATGAGA11180.7731727052054301No Hit
CATAGAGCTTTTTCTCCAGCCTCCAAGGGGTAAGGCGTACTGGTCTATGA11170.7724811374905773No Hit
CATAGATGTTTTATCAAAGGCATGCTCATTTATTTTAAAGGACTGGAAGC10640.7358280486033789No Hit
CATAGACGTGACTAAAAGCATGTTGGTGGAAGGGTAAGAGAGGGTCTATG8990.6217193756526671No Hit
CATAGACGTGACTAAAAGCATGTTGGTACACTGACGCTATACCACACTAC8640.597514505632819No Hit
CATAGAGTCTGAATGGTGGGAGAGTGAAAGAAATCTACAAAGGATTCTGG8260.5712349324684126No Hit
CATAGACTCATTGCACTTTCCACAACTGTCTGCCTCTAAGTAAACTGAAT8000.5532541718822398No Hit
CATAGACACAACAGAGAGAACGGGCTACACTGACGCTATACCACACTACA7580.5242083278584222No Hit
CATAGAGTGCCTGTTTCCTCATTCGTACACTGACGCTATACCACACTACA7250.5013865932682798No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGACGCTATACCACACTA7110.49170464526034063No Hit
CATAGATCAAGGAAAACACCAGCCCACACTGACGCTATACCACACTACAC7020.48548053582666545No Hit
CATAGACCAGCAGACACTCCCATCATCAGGAACCATGGGAGGTCTATGAG6700.4633503689513758No Hit
CATAGACCTCCGACAGTTTTTATGCACCTTCATTGTTGCTCTATGAGACC6050.4183984674859439No Hit
CATAGACCAACATTTCTTCTCCCAGGGTGGAAGGGTAAGAGAGGGTCTAT5390.3727549983056591No Hit
CATAGAAAATTTGTGATGGTAAATCATGGGACATGCCAGGAATCAATGTT5370.3713718628759535No Hit
CATAGAAGCCATGAACACAGGATTGAGGAGCTGAGTCATGGGGTCTATGA5110.3533911022897807No Hit
CATAGACCTCCGACAGTTTTTATGCACTGTCCCAACTGTCTCTGAAAAAT5060.3499332637155167No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACCAGTGAAGCAGAGCTTCCCA4860.3361019094184607No Hit
CATAGAGCTGTACTGCATCTTTTCTCAAACTGCTGGGGTTGCTCTATGAG4810.33264407084419667No Hit
CATAGACATTTAATCCTCACAATGACCCATACACTGGTGCATTACAGAGA4690.3243452582659631No Hit
CATAGATGTTTTATCAAAGGCATGCTCATACACCCACAGCCAGGAAAGAA4410.3049813622500847No Hit
CATAGATGGCCAAGATACGAGCACATACACTGACGCTATACCACACTACA4150.28700060166391195No Hit
CATAGATGGCCAAGATACGAGCACATAGAGATCCCACCATTTGCCATCTA4070.2814680599450895No Hit
CATAGACCTCCGACAGTTTTTATGCACCCACAGCCAGGAAAGAATCTATG3610.24965594506186073No Hit
CATAGATGTTTTATCAAAGGCATGCTCATACACTGGCTCAGGTATGTCTT3190.22061010103804315No Hit
CATAGAGTGCCTGTTTCCTCATTCGTCTGTTTCCCCACTCAAATACCATC3090.21369442388951515No Hit
CATAGATTGTGTGTCTCTTCTATATTAACAGTCATCTACAAAGGATTCTG3030.20954501760039834No Hit
CATAGAAGTCACGAGTTTTGTTGTTGTTGCATGACAGAGCGAGACTTCTA2960.20470404359642877No Hit
CATAGACTCATTGCACTTTCCACAACTGTACACTGCCCTCTCTGCTGTTC2840.19640523101819513No Hit
CATAGAAATGATGCTGAAGGGCCTGCACATAGAAATGATGCTGAAGTGAT2840.19640523101819513No Hit
CATAGAGTAGTTAAAACCCACAATGACAGTGTAGAGATCCCACCATTTGC2820.19502209558848954No Hit
CATAGAAATGATGCTGAAGTGATGGGCTAGATCTATGAGACCAAGTCTCT2810.19433052787363675No Hit
CATAGAAATGATGCTGAAGGGCCTGCTGCTGAAGTGATGGGCTAGATCTA2720.18810641843996156No Hit
CATAGACCAGCAGACACTCCCATCATCAAGGAAGTTTCTGTGAGATCTAT2660.18395701215084473No Hit
CATAGAAATGATGCTGAAGGGCCTGCACTGACGACATGGTTCTACACATA2620.18119074129143356No Hit
CATAGAACAGATGTTCTCCTTGTTTTCTATAACAAGGGGTAAGGCGTACT2620.18119074129143356No Hit
CATAGATGTGTAGCCTGGGTCTGACAAGGGGTAAGGCGTACTGGTCTATG2620.18119074129143356No Hit
CATAGAGATCCCACCATTTGCCATCTATGAGACCAAGTCTCTGCTACCGT2410.16666781927952476No Hit
CATAGATTGTGTGTCTCTTCTATATTAACAGTCTACAAAGGATTCTGGGC2280.15767743898643835No Hit
CATAGACCAACATTTCTTCTCCCAGGGTCTACCAGTTATTAGTTGAATGA2270.15698587127158556No Hit
CATAGAAATGATGCTGAAGGGTCTGTTTCCCCACTCAAATACCATCTATG2100.14522922011908798No Hit
CATAGACCCAAGACCCCAGCCTTTACCACATAGAGTGTCTGTTTCCCCAC1990.13762197525570716No Hit
CATAGACCTCCGACAGTTTTTATGCACTGTCATTGTCGCTGTCCCATCTA1990.13762197525570716No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACTGCTGGGGTTGCTCTATGAG1960.13554727211114878No Hit
CATAGAAGGACAAAGACATTTAGGACCCACCATTTGCCATAGAGATCCCA1950.13485570439629596No Hit
CATAGAAGCTGAGAAGACTTCCTAATGCACGACATGGTTCTACACATAGA1910.13208943353688476No Hit
CATAGAAATGATGCTGAAGGGCCTGCGATGCTGAAGTGATGGGCTAGATC1890.13070629810717915No Hit
CATAGAAAATTTGTGATGGTAAATCATGGGAGGTCTATCATCAGGAACCA1840.12724845953291516No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGACGATCATTGTTCACT1810.12517375638835676No Hit
CATAGACTCATTGCACTTTCCACAACTGTGTTCTACAAAGGATTCTGGGC1800.12448218867350397No Hit
CATAGAAAAAACAGTTGGCCAGGCATACACTGACGACATGGCTCAGGTAT1720.11894964695468156No Hit
CATAGAAATGATGCTGAAGGGCCTGCAGTCTTTGGAGGAATCTATGAGAC1620.11203396980615359No Hit
CATAGACCAACATTTCTTCTCCCAGGGTACACTGACGACATGGCTCAGGT1610.11134240209130077No Hit
CATAGAGCTGTACTGCATCTTTTCTCAGACACACTGTCATTGTCGCTGTC1610.11134240209130077No Hit
CATAGAGTCTGAATGGTGGGAGAGTGAAATGGTGGAAGGGTAAGAGAGGG1590.10995926666159517No Hit
CATAGAGTCTGAATGGTGGGAGAGTGCTGAAGTGATGGGCTAGATCTATG1580.10926769894674238No Hit
CATAGACGTGACTAAAAGCATGTTGGTGTTTGCAGATTTGGACCTGCGAG1500.10373515722791997No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)